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The map label for this gene is paaI [H]

Identifier: 57234285

GI number: 57234285

Start: 858550

End: 858960

Strand: Direct

Name: paaI [H]

Synonym: DET0937

Alternate gene names: 57234285

Gene position: 858550-858960 (Clockwise)

Preceding gene: 57234286

Following gene: 57234258

Centisome position: 58.42

GC content: 46.23

Gene sequence:

>411_bases
ATGACACACCCTGATGCTGAAGAAAATATTAAGCTTCTGCATGAAAAAAGCCGCACTGAACCTGCCCTGAATTTTCTGGG
CATAAAAATACTGGAATTGAAACCCGGTTATTCCAAGCTGAGTATCAAACTCAAACCGGAGTTTATAAATGCCTACGGCA
TAATTTTTGGCGGAATCACCATGTCTTTGGCAGATGAGGCATTCGGTTATGCAGTGAACAGTCTGAAACTGCCTACGGTA
GCCGCCCAGTTCAATATCCACTTTCTGTCAGCCCCGGATAATGATGACGAACTAACTGCGGAAGCCAAAGTAGTCAAATC
AGGCCGCCGTCTGGCTGTTGCCGAAGTAGAGGTGACCAATGCCAAAGGCAAACTGATAGCTAAAGTAAGTGCCAGCGGCG
TTCCGCTTTAA

Upstream 100 bases:

>100_bases
GCTATTTTGACCGTGACAAAGAATATGATAAACTGGCAGATATAGTCAGGGCAAGCATAGACATGAACAAGATTTATGAT
ATTTTGAACGAGGGAATAAG

Downstream 100 bases:

>100_bases
ACCCGCCCGCCAGGTAGTTTATTCATCACCCAGATAGGCCGCTATCACGTGCTCATCATTCTTTATTTCTTCAGGCCTGC
CCTCAGCTATTTTGCTGCCA

Product: thioesterase family protein

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MTHPDAEENIKLLHEKSRTEPALNFLGIKILELKPGYSKLSIKLKPEFINAYGIIFGGITMSLADEAFGYAVNSLKLPTV
AAQFNIHFLSAPDNDDELTAEAKVVKSGRRLAVAEVEVTNAKGKLIAKVSASGVPL

Sequences:

>Translated_136_residues
MTHPDAEENIKLLHEKSRTEPALNFLGIKILELKPGYSKLSIKLKPEFINAYGIIFGGITMSLADEAFGYAVNSLKLPTV
AAQFNIHFLSAPDNDDELTAEAKVVKSGRRLAVAEVEVTNAKGKLIAKVSASGVPL
>Mature_135_residues
THPDAEENIKLLHEKSRTEPALNFLGIKILELKPGYSKLSIKLKPEFINAYGIIFGGITMSLADEAFGYAVNSLKLPTVA
AQFNIHFLSAPDNDDELTAEAKVVKSGRRLAVAEVEVTNAKGKLIAKVSASGVPL

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=127, Percent_Identity=33.0708661417323, Blast_Score=74, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 14677; Mature: 14546

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPDAEENIKLLHEKSRTEPALNFLGIKILELKPGYSKLSIKLKPEFINAYGIIFGGIT
CCCCCCCHHHHHHHHCCCCCCCCHHHCEEEEEECCCCEEEEEEECCCCCCEEEEEECCEE
MSLADEAFGYAVNSLKLPTVAAQFNIHFLSAPDNDDELTAEAKVVKSGRRLAVAEVEVTN
EEHHHHHHHHEECCEECCEEEEEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEEEC
AKGKLIAKVSASGVPL
CCCEEEEEEECCCCCC
>Mature Secondary Structure 
THPDAEENIKLLHEKSRTEPALNFLGIKILELKPGYSKLSIKLKPEFINAYGIIFGGIT
CCCCCCHHHHHHHHCCCCCCCCHHHCEEEEEECCCCEEEEEEECCCCCCEEEEEECCEE
MSLADEAFGYAVNSLKLPTVAAQFNIHFLSAPDNDDELTAEAKVVKSGRRLAVAEVEVTN
EEHHHHHHHHEECCEECCEEEEEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEEEEC
AKGKLIAKVSASGVPL
CCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]