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The map label for this gene is pnp

Identifier: 57234211

GI number: 57234211

Start: 889292

End: 891454

Strand: Direct

Name: pnp

Synonym: DET0970

Alternate gene names: 57234211

Gene position: 889292-891454 (Clockwise)

Preceding gene: 57234220

Following gene: 57234210

Centisome position: 60.51

GC content: 50.35

Gene sequence:

>2163_bases
TTGATAACTGCCAACTCATTTGAACGAACTATTGGCGGCAGAAAACTTACTATAGAGAGCGGGAAAATGGCCCGCCTTGC
AGATGCCGCAGTAACTATCCGCTATGCGGATACCGAATTACTGGTAACACTTTGTGCTGCCAAAAAACCGCGCGAAGGGG
TGGATTTCCTCCCCCTGACTATAGACTACGAAGAGCGGATGTATGCCGCAGGCAAAATCCCCGGCGGGTTTATCCGCCGC
GAAGGACGCCCCAGTGAACAGGCTATTTTGGCCGGACGTCTGACAGACAGACCTCTCCGCCCCCTTCTCCCCAAGGAGTG
GCGGAATGAACTTCAGATTATTATCACCGTAATTGCCTCAGACAAGGAAAATGACGCTGATATCTGGGGTGTGGTAGGTG
CTTCAACCGTTCTTACCATGTCACAAATACCCTACGAAGGCCCGGTGGGAGCCAGCCGCGTAGGTTATACAAACGGCGAA
TTCGTGCTTAACCCCACCTTTGCCCAGCTGGAACAAAGCCAGATGGATTTGGTGGTAGTCAGCACCCGTAAAGCCGTAGT
TATGATTGAAGCCGGGTCTAAGGAAATTCCCGAAGACCTTATGATTAAAGCGATTGAGTTTGCCCATCAGGCAAATCAGG
AGCTTATTGACCTGCAGGATGAAATCCGCGCCAAGCTGGGCAAGGAAAAACTGCCGGTGCCGGTGCTGGAAATACCCGAA
GAAGTCAAAGCAGCGGTAGCCGCCTTTGTAAAAGGGCGAGTAAACGAAGCCCTGAGCCATCAGGATAAAACCCTCCGTGA
AAATGCGGTTGAAGCCCTTCAGAGCGAACTGGTTGAAGCTTTGGCAGAGACATATGCCGAAGGCGATATTCTGGCCGCTT
ATGATAAAGAAATCAAAAAGGCTATCCGCTCAACTATACTTGAAAAAGATATCAGGGTAAACGGACGGGGTATTAAGCAG
CTCCGCCAGCTGGACGCTGAAACAGGTATTCTGCCCCGCGTTCACGGTTCAGCTTTGTTCACCCGGGGTGATACCCAGGT
TATGGCCATTACCACACTGGGTTCTTTGCAGGAAGCCCAGCAGCTGGACGGCTTGAGTGCCGAAGATACCAAGCGTTTTA
TGCTTCACTATAATTTTGCCCCCTTCTCTACCGGTGAAGTTAAGCGTTCCGGCTCCCCCGGACGGCGTGAAATCGGTCAT
GGTGCTTTAGCTGAAAGGGCTTTGGTGCCTGTCCTGCCCACTCCCGAAGAATTTCCCTATACCATTCGTATGGTAGCTGA
CGTAGTCGGTTCATCAGGCTCTACTTCTATGGGCAGTGTCTGTTCGTCCAGTTTGTCACTTATGGATGCAGGTGTACCAG
TTAAAAAAGCTGTTGCCGGTATTTCCATCGGCCTTATTACCGGTGAAAATGATACCTACTGCACCATTACTGATATTGAA
GGTATTGAAGATAACTACGGCGATATGGATTTCAAAGTTGCCGGTACCCGTGACGGCATAACCGCCATACAGGTGGATAT
GAAAGTCAAGGGTATCAGCTTTGACGTTATCCGTGACGCTATCTACCAGGCCAAAGAAGCCCGCTATACCATACTGGAAG
TAATGGACAAGGCTCTTGCCCAGCCCAAGACCGAGCTTAGCCCTTACGCCCCCAGAATGTACAAGATAAGCATTGACCCC
TCCAAGATTGGCAGTGTTATCGGCAGCGGCGGCAAGACCATCCGCTCTATTATTGAGCAGACCAATACCACCGTTGATAT
TGAAAATGACGGCACAGTGGTCATCGGTGCTACTGACGAAGCTTCGGCCCAAAAAGCTATAAAGATAATTGAAGACCTGA
CCAAAGATGTAGAAGCCGGCAGTGTTTATACCGGTAAAGTTACCCGGATAATGACCTTTGGTGCTTTTGTTGAAATCCTG
CCCGGCAAAGAGGGCATGGTACACATAAGCGAACTGGCCGACCACAGAGTGGAAAAAGTGGAAGACGTAGTTAAAGTGGG
TGACGAAATAACAGTAAAGGTAACTGAGATTGATTCACAGGGCAGAATAAACCTGTCACGCCGGGTCATTCTCAATCCCA
ATGCTGTTCCCATCTCCCGCAACCGTGATTCCCAGCCGCGCCGTTCCGGGCCTTTCCGCCCTCAAGACCGGTCTAACTCA
TAA

Upstream 100 bases:

>100_bases
GCAACAATAACGGGGCCGGTTATCAGGAGCTTATTGCCAATCTTGGCCTTAGAAAATAAATATTGCCAAAGGCAAATAAC
CCTGTAAGGAGATTACGTTA

Downstream 100 bases:

>100_bases
TATATTAGGAGGCGCTATGACACCTATAAAGGTTGTAGTTCACGGTGCCTCAGGCAAAATGGGCCAGGAGGTCTTAAAGA
CCCTGTGCCAGGAAAATAAC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MITANSFERTIGGRKLTIESGKMARLADAAVTIRYADTELLVTLCAAKKPREGVDFLPLTIDYEERMYAAGKIPGGFIRR
EGRPSEQAILAGRLTDRPLRPLLPKEWRNELQIIITVIASDKENDADIWGVVGASTVLTMSQIPYEGPVGASRVGYTNGE
FVLNPTFAQLEQSQMDLVVVSTRKAVVMIEAGSKEIPEDLMIKAIEFAHQANQELIDLQDEIRAKLGKEKLPVPVLEIPE
EVKAAVAAFVKGRVNEALSHQDKTLRENAVEALQSELVEALAETYAEGDILAAYDKEIKKAIRSTILEKDIRVNGRGIKQ
LRQLDAETGILPRVHGSALFTRGDTQVMAITTLGSLQEAQQLDGLSAEDTKRFMLHYNFAPFSTGEVKRSGSPGRREIGH
GALAERALVPVLPTPEEFPYTIRMVADVVGSSGSTSMGSVCSSSLSLMDAGVPVKKAVAGISIGLITGENDTYCTITDIE
GIEDNYGDMDFKVAGTRDGITAIQVDMKVKGISFDVIRDAIYQAKEARYTILEVMDKALAQPKTELSPYAPRMYKISIDP
SKIGSVIGSGGKTIRSIIEQTNTTVDIENDGTVVIGATDEASAQKAIKIIEDLTKDVEAGSVYTGKVTRIMTFGAFVEIL
PGKEGMVHISELADHRVEKVEDVVKVGDEITVKVTEIDSQGRINLSRRVILNPNAVPISRNRDSQPRRSGPFRPQDRSNS

Sequences:

>Translated_720_residues
MITANSFERTIGGRKLTIESGKMARLADAAVTIRYADTELLVTLCAAKKPREGVDFLPLTIDYEERMYAAGKIPGGFIRR
EGRPSEQAILAGRLTDRPLRPLLPKEWRNELQIIITVIASDKENDADIWGVVGASTVLTMSQIPYEGPVGASRVGYTNGE
FVLNPTFAQLEQSQMDLVVVSTRKAVVMIEAGSKEIPEDLMIKAIEFAHQANQELIDLQDEIRAKLGKEKLPVPVLEIPE
EVKAAVAAFVKGRVNEALSHQDKTLRENAVEALQSELVEALAETYAEGDILAAYDKEIKKAIRSTILEKDIRVNGRGIKQ
LRQLDAETGILPRVHGSALFTRGDTQVMAITTLGSLQEAQQLDGLSAEDTKRFMLHYNFAPFSTGEVKRSGSPGRREIGH
GALAERALVPVLPTPEEFPYTIRMVADVVGSSGSTSMGSVCSSSLSLMDAGVPVKKAVAGISIGLITGENDTYCTITDIE
GIEDNYGDMDFKVAGTRDGITAIQVDMKVKGISFDVIRDAIYQAKEARYTILEVMDKALAQPKTELSPYAPRMYKISIDP
SKIGSVIGSGGKTIRSIIEQTNTTVDIENDGTVVIGATDEASAQKAIKIIEDLTKDVEAGSVYTGKVTRIMTFGAFVEIL
PGKEGMVHISELADHRVEKVEDVVKVGDEITVKVTEIDSQGRINLSRRVILNPNAVPISRNRDSQPRRSGPFRPQDRSNS
>Mature_720_residues
MITANSFERTIGGRKLTIESGKMARLADAAVTIRYADTELLVTLCAAKKPREGVDFLPLTIDYEERMYAAGKIPGGFIRR
EGRPSEQAILAGRLTDRPLRPLLPKEWRNELQIIITVIASDKENDADIWGVVGASTVLTMSQIPYEGPVGASRVGYTNGE
FVLNPTFAQLEQSQMDLVVVSTRKAVVMIEAGSKEIPEDLMIKAIEFAHQANQELIDLQDEIRAKLGKEKLPVPVLEIPE
EVKAAVAAFVKGRVNEALSHQDKTLRENAVEALQSELVEALAETYAEGDILAAYDKEIKKAIRSTILEKDIRVNGRGIKQ
LRQLDAETGILPRVHGSALFTRGDTQVMAITTLGSLQEAQQLDGLSAEDTKRFMLHYNFAPFSTGEVKRSGSPGRREIGH
GALAERALVPVLPTPEEFPYTIRMVADVVGSSGSTSMGSVCSSSLSLMDAGVPVKKAVAGISIGLITGENDTYCTITDIE
GIEDNYGDMDFKVAGTRDGITAIQVDMKVKGISFDVIRDAIYQAKEARYTILEVMDKALAQPKTELSPYAPRMYKISIDP
SKIGSVIGSGGKTIRSIIEQTNTTVDIENDGTVVIGATDEASAQKAIKIIEDLTKDVEAGSVYTGKVTRIMTFGAFVEIL
PGKEGMVHISELADHRVEKVEDVVKVGDEITVKVTEIDSQGRINLSRRVILNPNAVPISRNRDSQPRRSGPFRPQDRSNS

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=708, Percent_Identity=35.5932203389831, Blast_Score=439, Evalue=1e-123,
Organism=Escherichia coli, GI145693187, Length=681, Percent_Identity=49.3392070484581, Blast_Score=664, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=104, Percent_Identity=39.4230769230769, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI115534063, Length=704, Percent_Identity=32.2443181818182, Blast_Score=340, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6320850, Length=100, Percent_Identity=40, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI281362905, Length=720, Percent_Identity=37.3611111111111, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24651641, Length=720, Percent_Identity=37.3611111111111, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24651643, Length=720, Percent_Identity=37.3611111111111, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI161079377, Length=665, Percent_Identity=37.1428571428571, Blast_Score=428, Evalue=1e-120,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_DEHE1 (Q3Z7V6)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181693.1
- HSSP:   Q91WJ8
- ProteinModelPortal:   Q3Z7V6
- STRING:   Q3Z7V6
- GeneID:   3229678
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0970
- NMPDR:   fig|243164.3.peg.384
- TIGR:   DET0970
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q3Z7V6
- ProtClustDB:   PRK11824
- BioCyc:   DETH243164:DET_0970-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 78469; Mature: 78469

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITANSFERTIGGRKLTIESGKMARLADAAVTIRYADTELLVTLCAAKKPREGVDFLPLT
CCCCCCHHHHCCCEEEEECCCCCEEEECEEEEEEECCHHHHHHHHHCCCCCCCCCEEEEE
IDYEERMYAAGKIPGGFIRREGRPSEQAILAGRLTDRPLRPLLPKEWRNELQIIITVIAS
EEHHHHEEECCCCCCHHHCCCCCCCHHHEEECCCCCCCCCCCCCHHHHCCEEEEEEEEEC
DKENDADIWGVVGASTVLTMSQIPYEGPVGASRVGYTNGEFVLNPTFAQLEQSQMDLVVV
CCCCCCCEEEECCHHHHEEHHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHCCCCEEEE
STRKAVVMIEAGSKEIPEDLMIKAIEFAHQANQELIDLQDEIRAKLGKEKLPVPVLEIPE
ECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHCCCH
EVKAAVAAFVKGRVNEALSHQDKTLRENAVEALQSELVEALAETYAEGDILAAYDKEIKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
AIRSTILEKDIRVNGRGIKQLRQLDAETGILPRVHGSALFTRGDTQVMAITTLGSLQEAQ
HHHHHHHHHHCEECCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEEEHHHHHHHH
QLDGLSAEDTKRFMLHYNFAPFSTGEVKRSGSPGRREIGHGALAERALVPVLPTPEEFPY
HHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHCCEECCCCCCCCCCH
TIRMVADVVGSSGSTSMGSVCSSSLSLMDAGVPVKKAVAGISIGLITGENDTYCTITDIE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCEEEEEEECCCCCEEEEEECC
GIEDNYGDMDFKVAGTRDGITAIQVDMKVKGISFDVIRDAIYQAKEARYTILEVMDKALA
CCCCCCCCEEEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
QPKTELSPYAPRMYKISIDPSKIGSVIGSGGKTIRSIIEQTNTTVDIENDGTVVIGATDE
CCCCCCCCCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCC
ASAQKAIKIIEDLTKDVEAGSVYTGKVTRIMTFGAFVEILPGKEGMVHISELADHRVEKV
HHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHEECCCCCCCEEHHHHHHHHHHHH
EDVVKVGDEITVKVTEIDSQGRINLSRRVILNPNAVPISRNRDSQPRRSGPFRPQDRSNS
HHHHHCCCCEEEEEEEECCCCCEEEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MITANSFERTIGGRKLTIESGKMARLADAAVTIRYADTELLVTLCAAKKPREGVDFLPLT
CCCCCCHHHHCCCEEEEECCCCCEEEECEEEEEEECCHHHHHHHHHCCCCCCCCCEEEEE
IDYEERMYAAGKIPGGFIRREGRPSEQAILAGRLTDRPLRPLLPKEWRNELQIIITVIAS
EEHHHHEEECCCCCCHHHCCCCCCCHHHEEECCCCCCCCCCCCCHHHHCCEEEEEEEEEC
DKENDADIWGVVGASTVLTMSQIPYEGPVGASRVGYTNGEFVLNPTFAQLEQSQMDLVVV
CCCCCCCEEEECCHHHHEEHHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHCCCCEEEE
STRKAVVMIEAGSKEIPEDLMIKAIEFAHQANQELIDLQDEIRAKLGKEKLPVPVLEIPE
ECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHCCCH
EVKAAVAAFVKGRVNEALSHQDKTLRENAVEALQSELVEALAETYAEGDILAAYDKEIKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
AIRSTILEKDIRVNGRGIKQLRQLDAETGILPRVHGSALFTRGDTQVMAITTLGSLQEAQ
HHHHHHHHHHCEECCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEEEHHHHHHHH
QLDGLSAEDTKRFMLHYNFAPFSTGEVKRSGSPGRREIGHGALAERALVPVLPTPEEFPY
HHCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHCCEECCCCCCCCCCH
TIRMVADVVGSSGSTSMGSVCSSSLSLMDAGVPVKKAVAGISIGLITGENDTYCTITDIE
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCEEEEEEECCCCCEEEEEECC
GIEDNYGDMDFKVAGTRDGITAIQVDMKVKGISFDVIRDAIYQAKEARYTILEVMDKALA
CCCCCCCCEEEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
QPKTELSPYAPRMYKISIDPSKIGSVIGSGGKTIRSIIEQTNTTVDIENDGTVVIGATDE
CCCCCCCCCCCEEEEEEECHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCC
ASAQKAIKIIEDLTKDVEAGSVYTGKVTRIMTFGAFVEILPGKEGMVHISELADHRVEKV
HHHHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHEECCCCCCCEEHHHHHHHHHHHH
EDVVKVGDEITVKVTEIDSQGRINLSRRVILNPNAVPISRNRDSQPRRSGPFRPQDRSNS
HHHHHCCCCEEEEEEEECCCCCEEEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA