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The map label for this gene is pyrB

Identifier: 57234050

GI number: 57234050

Start: 1094092

End: 1095087

Strand: Direct

Name: pyrB

Synonym: DET1199

Alternate gene names: 57234050

Gene position: 1094092-1095087 (Clockwise)

Preceding gene: 57234051

Following gene: 57234049

Centisome position: 74.44

GC content: 53.21

Gene sequence:

>996_bases
ATGACGAACACCCCCAAAACCCCCGCAGAAACTGAAACCCTGCTTTCCGAGGTACAGGGCTGGAAACACCGCCATGTGCT
GGATTTAGATAACTTCAGCCGCGAAGAACTGGACATGGTTATGCAGACAGCCGGCGTCATGCTGGATATTTTATCCCGCC
CGGTAAAGAAAGTGCCGGCCCTGAAAGGCAAGACTATAGCCACCCTCTTTTACGAACCCTCCACCCGCACCCGTTCATCA
TTTGAGCTGGCGGCCAAAAGTTTATCTGCTGATGTATTAAACCTGAATGTCTCCCAGAGCAGCGTTTCCAAGGGGGAAAG
CCTGCTGGATACGCTGGATACACTGGAAGCACTGGGGGCGGATATGGTAGTTATGCGCCACCCCCTGTCAGGAGCGCCTT
ATCTGGCGGCAAACCACTGCCGGGCAAACATTATAAACGCCGGTGACGGCTGGCATGCCCACCCCAGCCAGGCCCTGCTG
GATATATTTACCATACTAAGGCACAAACCAGCCCTGGAAGGGCTGAAAATAACCCTGATAGGGGATATAAAACACAGCCG
GGTAGCCCACTCCAATATCTGGGGGCTAAGCAAAATGGGGGCTGAAATCACCCTGTGCGCCCCCTATACCCTGCTGCCGG
AGGGCTTAAATACCGACAGCCAAATATTCCCCGAAGTAACGGTAAAAACAGATATAAAACAGGCAGTCAGCGGGGCTGAC
GTGGTAATGGGGCTGCGTCTTCAGCGTGAACGCCAGCAAAGCGGGCTTCTGCCGGGAATACGGGAATACGCCCAGTACTT
TCAGCTGAATGCAGAAATACTCAAACTGGCTAAACCGGATGCGCTGGTGATGCACCCCGGCCCGGTAAATGAAGATATAG
AACTTTCGCAATCTGTGGTGCACGGAGAGCAGTCTGTCATAAACGAACAGGTAAAAAACGGGGTAGCCATCCGCATGGCC
CTCTTTTACCTGTGCAGCGGAAGCAAGGAAATATAG

Upstream 100 bases:

>100_bases
TACCCAGCTCACGTGACGAGAAGATAAAAGTACGCCTGACCGAAACAGACGGACGGGACGAAATACTTATACTGGATAAT
GAAGCAGGTGAGGTATAAAC

Downstream 100 bases:

>100_bases
ATATGAAAATACTGATTAAAAACGGGCGCATCATAGACCCTGCCGGAGGCACAGATAAAGTGGCAGACCTGCTGCTGGAA
AACGGGCTGGTTGCCGGAAT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTNTPKTPAETETLLSEVQGWKHRHVLDLDNFSREELDMVMQTAGVMLDILSRPVKKVPALKGKTIATLFYEPSTRTRSS
FELAAKSLSADVLNLNVSQSSVSKGESLLDTLDTLEALGADMVVMRHPLSGAPYLAANHCRANIINAGDGWHAHPSQALL
DIFTILRHKPALEGLKITLIGDIKHSRVAHSNIWGLSKMGAEITLCAPYTLLPEGLNTDSQIFPEVTVKTDIKQAVSGAD
VVMGLRLQRERQQSGLLPGIREYAQYFQLNAEILKLAKPDALVMHPGPVNEDIELSQSVVHGEQSVINEQVKNGVAIRMA
LFYLCSGSKEI

Sequences:

>Translated_331_residues
MTNTPKTPAETETLLSEVQGWKHRHVLDLDNFSREELDMVMQTAGVMLDILSRPVKKVPALKGKTIATLFYEPSTRTRSS
FELAAKSLSADVLNLNVSQSSVSKGESLLDTLDTLEALGADMVVMRHPLSGAPYLAANHCRANIINAGDGWHAHPSQALL
DIFTILRHKPALEGLKITLIGDIKHSRVAHSNIWGLSKMGAEITLCAPYTLLPEGLNTDSQIFPEVTVKTDIKQAVSGAD
VVMGLRLQRERQQSGLLPGIREYAQYFQLNAEILKLAKPDALVMHPGPVNEDIELSQSVVHGEQSVINEQVKNGVAIRMA
LFYLCSGSKEI
>Mature_330_residues
TNTPKTPAETETLLSEVQGWKHRHVLDLDNFSREELDMVMQTAGVMLDILSRPVKKVPALKGKTIATLFYEPSTRTRSSF
ELAAKSLSADVLNLNVSQSSVSKGESLLDTLDTLEALGADMVVMRHPLSGAPYLAANHCRANIINAGDGWHAHPSQALLD
IFTILRHKPALEGLKITLIGDIKHSRVAHSNIWGLSKMGAEITLCAPYTLLPEGLNTDSQIFPEVTVKTDIKQAVSGADV
VMGLRLQRERQQSGLLPGIREYAQYFQLNAEILKLAKPDALVMHPGPVNEDIELSQSVVHGEQSVINEQVKNGVAIRMAL
FYLCSGSKEI

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=325, Percent_Identity=37.2307692307692, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI38788445, Length=309, Percent_Identity=25.2427184466019, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=33.1189710610932, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790703, Length=323, Percent_Identity=27.5541795665635, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786469, Length=317, Percent_Identity=26.4984227129338, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=290, Percent_Identity=36.2068965517241, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=327, Percent_Identity=33.9449541284404, Blast_Score=160, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24642586, Length=317, Percent_Identity=34.7003154574132, Blast_Score=154, Evalue=6e-38,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_DEHE1 (Q3Z787)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181912.1
- HSSP:   P0A786
- ProteinModelPortal:   Q3Z787
- SMR:   Q3Z787
- STRING:   Q3Z787
- GeneID:   3229516
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET1199
- NMPDR:   fig|243164.3.peg.1091
- TIGR:   DET1199
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   DETH243164:DET_1199-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 36249; Mature: 36118

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTPKTPAETETLLSEVQGWKHRHVLDLDNFSREELDMVMQTAGVMLDILSRPVKKVPA
CCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
LKGKTIATLFYEPSTRTRSSFELAAKSLSADVLNLNVSQSSVSKGESLLDTLDTLEALGA
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHHHHHHHHCCC
DMVVMRHPLSGAPYLAANHCRANIINAGDGWHAHPSQALLDIFTILRHKPALEGLKITLI
CEEEEECCCCCCCCEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
GDIKHSRVAHSNIWGLSKMGAEITLCAPYTLLPEGLNTDSQIFPEVTVKTDIKQAVSGAD
ECCCHHHHHHHHCCCHHHCCCEEEEECCCEECCCCCCCCCHHCCCEEEHHHHHHHHCCHH
VVMGLRLQRERQQSGLLPGIREYAQYFQLNAEILKLAKPDALVMHPGPVNEDIELSQSVV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
HGEQSVINEQVKNGVAIRMALFYLCSGSKEI
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TNTPKTPAETETLLSEVQGWKHRHVLDLDNFSREELDMVMQTAGVMLDILSRPVKKVPA
CCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
LKGKTIATLFYEPSTRTRSSFELAAKSLSADVLNLNVSQSSVSKGESLLDTLDTLEALGA
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCHHEEECCCHHHHHHHHHHHHHHHHHHHCCC
DMVVMRHPLSGAPYLAANHCRANIINAGDGWHAHPSQALLDIFTILRHKPALEGLKITLI
CEEEEECCCCCCCCEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
GDIKHSRVAHSNIWGLSKMGAEITLCAPYTLLPEGLNTDSQIFPEVTVKTDIKQAVSGAD
ECCCHHHHHHHHCCCHHHCCCEEEEECCCEECCCCCCCCCHHCCCEEEHHHHHHHHCCHH
VVMGLRLQRERQQSGLLPGIREYAQYFQLNAEILKLAKPDALVMHPGPVNEDIELSQSVV
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHH
HGEQSVINEQVKNGVAIRMALFYLCSGSKEI
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA