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The map label for this gene is carA [H]

Identifier: 57234040

GI number: 57234040

Start: 1096412

End: 1097506

Strand: Direct

Name: carA [H]

Synonym: DET1201

Alternate gene names: 57234040

Gene position: 1096412-1097506 (Clockwise)

Preceding gene: 57234049

Following gene: 57234039

Centisome position: 74.6

GC content: 51.87

Gene sequence:

>1095_bases
ATGACAAATACAGCATACCTGGTACTGGAGGACGGAACAGTATTCAGCGGCAAAAGCTTCGGGGCGGAAACTGAAGCCAT
AGGCGAAGTGGTCTTTAATACCAGCATGAACGGATATCAGGAAATGCTGACTGACCCTTCGTATACCGGCCAGATTATTG
TCCCCACTTACCCTCTTATCGGCAACTACGGGGTAAACCCCTTTGATAATGAATCCGCCTGCATCAGGGCAACCGCCTTT
GGGGTACATGAGGAATGCCTGCTGCCAAACCACTACCAGAATAACCAAACCATTCACTCCTTTCTGGAAGAATCCGGCAT
ACCCGGCATAAGCGGCATAGATACCCGCGCCATTACCCGTAAGCTGCGCTCTGCCGGGGTAATGAGGGGCATGATAACCA
CTACCAAAACCCCTGAAGAAGCCCTTAAAACTATCCGCAGCGCACCTGACTACGGGCAGATTGACTTTGTACGCAAGATA
AGCACTCCTTCAGCCTACGAATGGCAAAAGGAAAAACCCTCTTTTGCCGGGTTTCATATAGCGGTGCTGGACTGCGGGCT
TAAGTACTCTATACTTAACCAGCTGAAATCCCACGGCTGCAAAGTAACTGTTTTGCCCTGCACTGCCAGCCCGCAAGACA
TAGATGCCCTTAATCCAGACGGGGTGCTTCTTTCACCCGGCCCCGGCAACCCGGAACTGCTGGACTATCTGGTAAATACA
GTCAGATACGCCTGTGAAAAATACCCGGTCATGGGCATCTGTCTGGGAAACCAGCTGATAGGCAAAGCCTTCGGGGCTAA
AACATTCAAGCTGAAGTTCGGCCACCGGGGCGGAAACCACCCGGTTAAAGACCTTGCCAGCGGTCGGGTATATATAACCT
CGCAAAATCACGGCTACTCCCTTGACCCCGCCACCCTGAAAGAAGGGCTGGAGGTCAGCCACATAAACCTGAATGACGGC
ACGGTGGAAGGGCTTCGCCACCGCGAACTGCCGGTGTTCTCCATACAGTACCACTCCGAAGCTTCACCCGGACCGATGGA
TTCCACCTACCTGTTCAAACAGTTTGTGGAAATGATAAAACAAACCAAAAAGTAG

Upstream 100 bases:

>100_bases
GCAGGCTGAAAGGCAGGGTAAAAACCACCATTGCCTGCGGCCGGATAGCATACCGGGAATAACAGACTTGCCAGTTTAGA
AAGTGAGAAGGATAGATTAA

Downstream 100 bases:

>100_bases
CTGACACCACTCAGAGATGGATTAGTTGAGAAAGCAAAAAGGAATATGCGAAGTGATGAATAAACCCAAAAAAGTTCTTA
TTATCGGCTCCGGCCCGATT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MTNTAYLVLEDGTVFSGKSFGAETEAIGEVVFNTSMNGYQEMLTDPSYTGQIIVPTYPLIGNYGVNPFDNESACIRATAF
GVHEECLLPNHYQNNQTIHSFLEESGIPGISGIDTRAITRKLRSAGVMRGMITTTKTPEEALKTIRSAPDYGQIDFVRKI
STPSAYEWQKEKPSFAGFHIAVLDCGLKYSILNQLKSHGCKVTVLPCTASPQDIDALNPDGVLLSPGPGNPELLDYLVNT
VRYACEKYPVMGICLGNQLIGKAFGAKTFKLKFGHRGGNHPVKDLASGRVYITSQNHGYSLDPATLKEGLEVSHINLNDG
TVEGLRHRELPVFSIQYHSEASPGPMDSTYLFKQFVEMIKQTKK

Sequences:

>Translated_364_residues
MTNTAYLVLEDGTVFSGKSFGAETEAIGEVVFNTSMNGYQEMLTDPSYTGQIIVPTYPLIGNYGVNPFDNESACIRATAF
GVHEECLLPNHYQNNQTIHSFLEESGIPGISGIDTRAITRKLRSAGVMRGMITTTKTPEEALKTIRSAPDYGQIDFVRKI
STPSAYEWQKEKPSFAGFHIAVLDCGLKYSILNQLKSHGCKVTVLPCTASPQDIDALNPDGVLLSPGPGNPELLDYLVNT
VRYACEKYPVMGICLGNQLIGKAFGAKTFKLKFGHRGGNHPVKDLASGRVYITSQNHGYSLDPATLKEGLEVSHINLNDG
TVEGLRHRELPVFSIQYHSEASPGPMDSTYLFKQFVEMIKQTKK
>Mature_363_residues
TNTAYLVLEDGTVFSGKSFGAETEAIGEVVFNTSMNGYQEMLTDPSYTGQIIVPTYPLIGNYGVNPFDNESACIRATAFG
VHEECLLPNHYQNNQTIHSFLEESGIPGISGIDTRAITRKLRSAGVMRGMITTTKTPEEALKTIRSAPDYGQIDFVRKIS
TPSAYEWQKEKPSFAGFHIAVLDCGLKYSILNQLKSHGCKVTVLPCTASPQDIDALNPDGVLLSPGPGNPELLDYLVNTV
RYACEKYPVMGICLGNQLIGKAFGAKTFKLKFGHRGGNHPVKDLASGRVYITSQNHGYSLDPATLKEGLEVSHINLNDGT
VEGLRHRELPVFSIQYHSEASPGPMDSTYLFKQFVEMIKQTKK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=370, Percent_Identity=40.2702702702703, Blast_Score=266, Evalue=2e-71,
Organism=Homo sapiens, GI21361331, Length=373, Percent_Identity=36.4611260053619, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI169790915, Length=373, Percent_Identity=36.4611260053619, Blast_Score=242, Evalue=5e-64,
Organism=Escherichia coli, GI1786215, Length=379, Percent_Identity=44.0633245382586, Blast_Score=334, Evalue=4e-93,
Organism=Escherichia coli, GI1789760, Length=135, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=373, Percent_Identity=38.0697050938338, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=39.8477157360406, Blast_Score=256, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6324878, Length=376, Percent_Identity=38.2978723404255, Blast_Score=240, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6322638, Length=175, Percent_Identity=28, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24642586, Length=379, Percent_Identity=43.5356200527704, Blast_Score=274, Evalue=9e-74,
Organism=Drosophila melanogaster, GI45555749, Length=383, Percent_Identity=43.0809399477807, Blast_Score=273, Evalue=9e-74,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39923; Mature: 39792

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTAYLVLEDGTVFSGKSFGAETEAIGEVVFNTSMNGYQEMLTDPSYTGQIIVPTYPLI
CCCEEEEEEECCEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEECCCCC
GNYGVNPFDNESACIRATAFGVHEECLLPNHYQNNQTIHSFLEESGIPGISGIDTRAITR
CCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHH
KLRSAGVMRGMITTTKTPEEALKTIRSAPDYGQIDFVRKISTPSAYEWQKEKPSFAGFHI
HHHHHHHHHHHEECCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
AVLDCGLKYSILNQLKSHGCKVTVLPCTASPQDIDALNPDGVLLSPGPGNPELLDYLVNT
EEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
VRYACEKYPVMGICLGNQLIGKAFGAKTFKLKFGHRGGNHPVKDLASGRVYITSQNHGYS
HHHHHHHCCEEEEEECCHHHHHHHCCEEEEEEECCCCCCCCHHHHCCCEEEEEECCCCCC
LDPATLKEGLEVSHINLNDGTVEGLRHRELPVFSIQYHSEASPGPMDSTYLFKQFVEMIK
CCHHHHHCCCEEEEEECCCCHHHCHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
QTKK
HHCC
>Mature Secondary Structure 
TNTAYLVLEDGTVFSGKSFGAETEAIGEVVFNTSMNGYQEMLTDPSYTGQIIVPTYPLI
CCEEEEEEECCEEECCCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEEECCCCC
GNYGVNPFDNESACIRATAFGVHEECLLPNHYQNNQTIHSFLEESGIPGISGIDTRAITR
CCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHH
KLRSAGVMRGMITTTKTPEEALKTIRSAPDYGQIDFVRKISTPSAYEWQKEKPSFAGFHI
HHHHHHHHHHHEECCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEE
AVLDCGLKYSILNQLKSHGCKVTVLPCTASPQDIDALNPDGVLLSPGPGNPELLDYLVNT
EEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
VRYACEKYPVMGICLGNQLIGKAFGAKTFKLKFGHRGGNHPVKDLASGRVYITSQNHGYS
HHHHHHHCCEEEEEECCHHHHHHHCCEEEEEEECCCCCCCCHHHHCCCEEEEEECCCCCC
LDPATLKEGLEVSHINLNDGTVEGLRHRELPVFSIQYHSEASPGPMDSTYLFKQFVEMIK
CCHHHHHCCCEEEEEECCCCHHHCHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
QTKK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA