Click here to switch to the map view.

The map label for this gene is purU [H]

Identifier: 57234014

GI number: 57234014

Start: 1132917

End: 1133771

Strand: Direct

Name: purU [H]

Synonym: DET1237

Alternate gene names: 57234014

Gene position: 1132917-1133771 (Clockwise)

Preceding gene: 57234016

Following gene: 57234013

Centisome position: 77.08

GC content: 44.91

Gene sequence:

>855_bases
ATGCCCAGCGCTATACTTAAAATTCACTGCACAGATAAAAAAGGCATTATTTCCAGTATTTCCAGCTTTATCTGCCGCAA
TAACGGCAATATTATTACCCTGGACGAATTTGTAGACCACCCGTCCAATACTTTTTTTATGCGCCTGGAATGGGATATTT
CTGCTTTTGCACTTAGCCGTGAACAGATGGAAGCCGAGATTGCACTAATGGGGCAGGAATACTGCTACGCAGACAACTGG
CAGATATTCTACTCTGACCGCAAACCCCGGCTGGCAGTATTTGTATCTAAATATGACCACTGTCTGTGGGATATAATGCT
GAGGTATAAAGCCGGAGAACTTAAGTGTGATATTCCCCTGATTATCAGCAACCACCCTAATTTAAAACCGGTAGCCGACT
TATTCGGCATTGATTACAAAGTAGTCAAAGTCACCCCGGATAACAAGCTTGAAGCCGAAAATGAGCAGACCTGTCTGATC
AATGAGTACAGTATAGACTTCATGATACTGGCCAGATATATGCAGGTGCTTTCACCCGAATTTGTAGCCCGCTTTGAAAA
CAGGATAATAAATATCCACCATTCGTTCCTGCCGGCTTTTGAGGGGGCGCGCCCCTACCACCAGGCGATTGAACGCGGGG
TAAAACTGGTGGGTGCTACCGCCCATTTTGTAAATAACAATCTGGACAAAGGCCCTATAATCTGCCAGTCCACCATGCCC
ATAAGCCATGAGGATAGCGTAGAAGACCTTATGGTAAAGGGCAGAGATATTGAAAAACTGGTGCTTTCACAGGCAATGAA
GGTATTTTTAGACCACCACATCTTCGTGCACAACAACCGCACTATTATCCTCTAG

Upstream 100 bases:

>100_bases
CCATTTCAAGTTTTAGCCGCCCCAAAATGCGTAGCTGAAAAGATTGTCTTGACGATAAATAACTGCTAGAGTATGTAATA
ACTTTTTTAAAGAGAAGAAT

Downstream 100 bases:

>100_bases
GTATTGCCAGCCGGAGAAAATCTGAAACCGGCTTAAGGTATACCCCAAAATGCCGCCTGATGCTTTATATTTATGGTAAG
GTTTTGTTATACTTACTTAG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MPSAILKIHCTDKKGIISSISSFICRNNGNIITLDEFVDHPSNTFFMRLEWDISAFALSREQMEAEIALMGQEYCYADNW
QIFYSDRKPRLAVFVSKYDHCLWDIMLRYKAGELKCDIPLIISNHPNLKPVADLFGIDYKVVKVTPDNKLEAENEQTCLI
NEYSIDFMILARYMQVLSPEFVARFENRIINIHHSFLPAFEGARPYHQAIERGVKLVGATAHFVNNNLDKGPIICQSTMP
ISHEDSVEDLMVKGRDIEKLVLSQAMKVFLDHHIFVHNNRTIIL

Sequences:

>Translated_284_residues
MPSAILKIHCTDKKGIISSISSFICRNNGNIITLDEFVDHPSNTFFMRLEWDISAFALSREQMEAEIALMGQEYCYADNW
QIFYSDRKPRLAVFVSKYDHCLWDIMLRYKAGELKCDIPLIISNHPNLKPVADLFGIDYKVVKVTPDNKLEAENEQTCLI
NEYSIDFMILARYMQVLSPEFVARFENRIINIHHSFLPAFEGARPYHQAIERGVKLVGATAHFVNNNLDKGPIICQSTMP
ISHEDSVEDLMVKGRDIEKLVLSQAMKVFLDHHIFVHNNRTIIL
>Mature_283_residues
PSAILKIHCTDKKGIISSISSFICRNNGNIITLDEFVDHPSNTFFMRLEWDISAFALSREQMEAEIALMGQEYCYADNWQ
IFYSDRKPRLAVFVSKYDHCLWDIMLRYKAGELKCDIPLIISNHPNLKPVADLFGIDYKVVKVTPDNKLEAENEQTCLIN
EYSIDFMILARYMQVLSPEFVARFENRIINIHHSFLPAFEGARPYHQAIERGVKLVGATAHFVNNNLDKGPIICQSTMPI
SHEDSVEDLMVKGRDIEKLVLSQAMKVFLDHHIFVHNNRTIIL

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=188, Percent_Identity=29.7872340425532, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI209869995, Length=188, Percent_Identity=29.7872340425532, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI209869993, Length=188, Percent_Identity=29.7872340425532, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1787483, Length=280, Percent_Identity=43.2142857142857, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1788846, Length=198, Percent_Identity=28.2828282828283, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=169, Percent_Identity=34.3195266272189, Blast_Score=111, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24582400, Length=196, Percent_Identity=30.6122448979592, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32709; Mature: 32578

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSAILKIHCTDKKGIISSISSFICRNNGNIITLDEFVDHPSNTFFMRLEWDISAFALSR
CCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEHHHHCCCCCEEEEEEEECHHHHHHHH
EQMEAEIALMGQEYCYADNWQIFYSDRKPRLAVFVSKYDHCLWDIMLRYKAGELKCDIPL
HHHHHHEEEECCCCEEECCEEEEEECCCCEEEEEEEHHHHHHHHHHHHCCCCCEEECEEE
IISNHPNLKPVADLFGIDYKVVKVTPDNKLEAENEQTCLINEYSIDFMILARYMQVLSPE
EECCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCHH
FVARFENRIINIHHSFLPAFEGARPYHQAIERGVKLVGATAHFVNNNLDKGPIICQSTMP
HHHHHHHHEEEEEHHHCCCCCCCCHHHHHHHHCCEEEEHHHHHHCCCCCCCCEEEECCCC
ISHEDSVEDLMVKGRDIEKLVLSQAMKVFLDHHIFVHNNRTIIL
CCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCEEEEECCEEEEC
>Mature Secondary Structure 
PSAILKIHCTDKKGIISSISSFICRNNGNIITLDEFVDHPSNTFFMRLEWDISAFALSR
CCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEHHHHCCCCCEEEEEEEECHHHHHHHH
EQMEAEIALMGQEYCYADNWQIFYSDRKPRLAVFVSKYDHCLWDIMLRYKAGELKCDIPL
HHHHHHEEEECCCCEEECCEEEEEECCCCEEEEEEEHHHHHHHHHHHHCCCCCEEECEEE
IISNHPNLKPVADLFGIDYKVVKVTPDNKLEAENEQTCLINEYSIDFMILARYMQVLSPE
EECCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCHH
FVARFENRIINIHHSFLPAFEGARPYHQAIERGVKLVGATAHFVNNNLDKGPIICQSTMP
HHHHHHHHEEEEEHHHCCCCCCCCHHHHHHHHCCEEEEHHHHHHCCCCCCCCEEEECCCC
ISHEDSVEDLMVKGRDIEKLVLSQAMKVFLDHHIFVHNNRTIIL
CCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]