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The map label for this gene is argG

Identifier: 57233965

GI number: 57233965

Start: 1146867

End: 1148087

Strand: Direct

Name: argG

Synonym: DET1260

Alternate gene names: 57233965

Gene position: 1146867-1148087 (Clockwise)

Preceding gene: 57233966

Following gene: 57233964

Centisome position: 78.03

GC content: 52.91

Gene sequence:

>1221_bases
ATGTCTGAAAAAGTTGTACTGGCATATTCCGGCGGGCTGGATACATCGGCCGCCGTCAAATGGCTTCAGGAAAAATACGG
CATGGACGTTATTGCCGTTACCATAGACGTGGGTAATGAGAAAGACTTTACCCTGATAAAAGAAAAAGCCCTGAAGGTAG
GGGCTAAAAAGGCCTACGTACGGGATGTACGCAAGGAATTTGCCGAGGATTATATCTGGAAGGCTATCAAGGCCAATTCC
ATGTACGAGGGTGTCTATCCGCTGGCTACCGCCCTTGCCCGCCCCCTGATTGCCAAGGTTATGGTAGACATAGCCATGGA
AGAAGGGGCTACCGCCATTGCCCACGGCTGCACCGGCAAAGGCAATGACCAGGTACGTTTTGACGTGGGCATAAACACCC
TTGCCCCCCATTTGAAGATTATTGCTCCCGCCCGCCAGTGGGGCATGACCCGTGAGCAAACTATGGAATACGCCCAGAAA
TGGGGCATTCCCGTACCTATTTCAGTCAAAAACCCTTTCTCCATAGATGAAAATCTGTGGGGGCGGAGTATAGAGTGCGG
TCTGCTGGAAGACCCCTGGAACGAGCCTATTCCCGAAGTATTTGCCTGGACCCGCCCGGTGGAAGCAACCCCGGACGCAC
CTGAGTACCTGGAAGTAGAGTTTGAACAGGGCGTGCCGGTAGCTGTAAACGGGGAAAAACTGTCTCCTTTAGCCCTTATA
CAGAAAGTGCATGATATTGCCGGACTGCATGGGGTAGGCCGTATTGACCACGTGGAAAACCGTCTGGTAGGCATTAAATC
CCGCGAGATTTATGAAGCCCCGGCGGCGGTGGTGCTGATTGCCGCCCACCAGGCTCTGGAAGCCATGACCCTTTCCAAGA
GCCAGTTACGCTTTAAGCAGATGGTGGAAGCCACCTATTCGGATATTATTTATAACGGGCTGTGGTTCTCTGCCCTGCGG
CAGGATTTGGACGCTTTTATAGAATCCAGCCAGCGCTTTGTCAGCGGCACAGTCCGCTTAAAGCTTTCCAAGGGCAGCTT
CCGGGTAGTGGGACGCAAATCCCCCTATTCCCTGTACCACAAGGGTATGGCCACCTATGATAAGGGAGACCAGTTTGACC
CGTCTTCGGCAGTGGGTTTCATAACCCTGTGGGGACTTCAGGCCAAACTGCAGGCCCAGCTCCAGCCTATTCTGGAAGAA
GAAAAGGGGAATAAATCCTAG

Upstream 100 bases:

>100_bases
GATAACTGATTTTTTAACAATAAACTTAAAAACGTAAAAAATGACTGGACTTCAAACCAAGGTTAGTAGATAATAAAACA
ATTGCTTAAGGAAGGTTTTT

Downstream 100 bases:

>100_bases
ATGAGCCATATCCGCAGCCGCTTTTCAAAACCGGCAGATGAACTTGTGGTCAGATATACCACCTCGCTGCCGTTTGACTG
GCGCTTATACCAGGAAGATA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSEKVVLAYSGGLDTSAAVKWLQEKYGMDVIAVTIDVGNEKDFTLIKEKALKVGAKKAYVRDVRKEFAEDYIWKAIKANS
MYEGVYPLATALARPLIAKVMVDIAMEEGATAIAHGCTGKGNDQVRFDVGINTLAPHLKIIAPARQWGMTREQTMEYAQK
WGIPVPISVKNPFSIDENLWGRSIECGLLEDPWNEPIPEVFAWTRPVEATPDAPEYLEVEFEQGVPVAVNGEKLSPLALI
QKVHDIAGLHGVGRIDHVENRLVGIKSREIYEAPAAVVLIAAHQALEAMTLSKSQLRFKQMVEATYSDIIYNGLWFSALR
QDLDAFIESSQRFVSGTVRLKLSKGSFRVVGRKSPYSLYHKGMATYDKGDQFDPSSAVGFITLWGLQAKLQAQLQPILEE
EKGNKS

Sequences:

>Translated_406_residues
MSEKVVLAYSGGLDTSAAVKWLQEKYGMDVIAVTIDVGNEKDFTLIKEKALKVGAKKAYVRDVRKEFAEDYIWKAIKANS
MYEGVYPLATALARPLIAKVMVDIAMEEGATAIAHGCTGKGNDQVRFDVGINTLAPHLKIIAPARQWGMTREQTMEYAQK
WGIPVPISVKNPFSIDENLWGRSIECGLLEDPWNEPIPEVFAWTRPVEATPDAPEYLEVEFEQGVPVAVNGEKLSPLALI
QKVHDIAGLHGVGRIDHVENRLVGIKSREIYEAPAAVVLIAAHQALEAMTLSKSQLRFKQMVEATYSDIIYNGLWFSALR
QDLDAFIESSQRFVSGTVRLKLSKGSFRVVGRKSPYSLYHKGMATYDKGDQFDPSSAVGFITLWGLQAKLQAQLQPILEE
EKGNKS
>Mature_405_residues
SEKVVLAYSGGLDTSAAVKWLQEKYGMDVIAVTIDVGNEKDFTLIKEKALKVGAKKAYVRDVRKEFAEDYIWKAIKANSM
YEGVYPLATALARPLIAKVMVDIAMEEGATAIAHGCTGKGNDQVRFDVGINTLAPHLKIIAPARQWGMTREQTMEYAQKW
GIPVPISVKNPFSIDENLWGRSIECGLLEDPWNEPIPEVFAWTRPVEATPDAPEYLEVEFEQGVPVAVNGEKLSPLALIQ
KVHDIAGLHGVGRIDHVENRLVGIKSREIYEAPAAVVLIAAHQALEAMTLSKSQLRFKQMVEATYSDIIYNGLWFSALRQ
DLDAFIESSQRFVSGTVRLKLSKGSFRVVGRKSPYSLYHKGMATYDKGDQFDPSSAVGFITLWGLQAKLQAQLQPILEEE
KGNKS

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=410, Percent_Identity=43.6585365853659, Blast_Score=325, Evalue=4e-89,
Organism=Homo sapiens, GI16950633, Length=410, Percent_Identity=43.6585365853659, Blast_Score=325, Evalue=4e-89,
Organism=Escherichia coli, GI1789563, Length=413, Percent_Identity=30.2663438256659, Blast_Score=145, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6324514, Length=407, Percent_Identity=40.5405405405405, Blast_Score=280, Evalue=4e-76,
Organism=Drosophila melanogaster, GI21358151, Length=396, Percent_Identity=42.4242424242424, Blast_Score=291, Evalue=4e-79,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_DEHE1 (Q3Z727)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_181972.1
- HSSP:   Q9X2A1
- ProteinModelPortal:   Q3Z727
- SMR:   Q3Z727
- STRING:   Q3Z727
- GeneID:   3229430
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET1260
- NMPDR:   fig|243164.3.peg.1253
- TIGR:   DET1260
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- PhylomeDB:   Q3Z727
- ProtClustDB:   PRK00509
- BioCyc:   DETH243164:DET_1260-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45044; Mature: 44913

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 86-86 BINDING 116-116 BINDING 118-118 BINDING 122-122 BINDING 122-122 BINDING 123-123 BINDING 126-126 BINDING 174-174 BINDING 183-183 BINDING 259-259 BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKVVLAYSGGLDTSAAVKWLQEKYGMDVIAVTIDVGNEKDFTLIKEKALKVGAKKAYV
CCCCEEEEECCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHCHHHHHH
RDVRKEFAEDYIWKAIKANSMYEGVYPLATALARPLIAKVMVDIAMEEGATAIAHGCTGK
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
GNDQVRFDVGINTLAPHLKIIAPARQWGMTREQTMEYAQKWGIPVPISVKNPFSIDENLW
CCCEEEEEECCHHHCCCCEEEECHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
GRSIECGLLEDPWNEPIPEVFAWTRPVEATPDAPEYLEVEFEQGVPVAVNGEKLSPLALI
CCCEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCHHHHH
QKVHDIAGLHGVGRIDHVENRLVGIKSREIYEAPAAVVLIAAHQALEAMTLSKSQLRFKQ
HHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHCCHHEEEEHHHHHHHHHHHHHHHHHHHH
MVEATYSDIIYNGLWFSALRQDLDAFIESSQRFVSGTVRLKLSKGSFRVVGRKSPYSLYH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEEEEEEEECCCEEEEECCCCHHHHH
KGMATYDKGDQFDPSSAVGFITLWGLQAKLQAQLQPILEEEKGNKS
CCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SEKVVLAYSGGLDTSAAVKWLQEKYGMDVIAVTIDVGNEKDFTLIKEKALKVGAKKAYV
CCCEEEEECCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHCHHHHHH
RDVRKEFAEDYIWKAIKANSMYEGVYPLATALARPLIAKVMVDIAMEEGATAIAHGCTGK
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
GNDQVRFDVGINTLAPHLKIIAPARQWGMTREQTMEYAQKWGIPVPISVKNPFSIDENLW
CCCEEEEEECCHHHCCCCEEEECHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCC
GRSIECGLLEDPWNEPIPEVFAWTRPVEATPDAPEYLEVEFEQGVPVAVNGEKLSPLALI
CCCEECCCCCCCCCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEECCCCCCHHHHH
QKVHDIAGLHGVGRIDHVENRLVGIKSREIYEAPAAVVLIAAHQALEAMTLSKSQLRFKQ
HHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHCCHHEEEEHHHHHHHHHHHHHHHHHHHH
MVEATYSDIIYNGLWFSALRQDLDAFIESSQRFVSGTVRLKLSKGSFRVVGRKSPYSLYH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEEEEEEEEECCCEEEEECCCCHHHHH
KGMATYDKGDQFDPSSAVGFITLWGLQAKLQAQLQPILEEEKGNKS
CCCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA