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The map label for this gene is gatA

Identifier: 57233937

GI number: 57233937

Start: 1207733

End: 1209193

Strand: Direct

Name: gatA

Synonym: DET1335

Alternate gene names: 57233937

Gene position: 1207733-1209193 (Clockwise)

Preceding gene: 57233938

Following gene: 57233936

Centisome position: 82.17

GC content: 51.95

Gene sequence:

>1461_bases
ATGACAGATTTAATCAAGCTGACCATTGCCCAGTCCCACAAACTGCTGAAAGAACGGAAACTGTCATCCGCCGAGCTTAC
CCGAGCCCATCTGGACAGGATAGAAAAACTTGAGCCTGAGATAAAGGCTTTTATGACTGTCTGCCCGGAAAGTGCTTTAG
CCCAGGCTAAAGCGGCTGACGAAGCTATAAAACAGGGGCATATCCGCCCGCTGACAGGTATCCCCATGGCCCTTAAAGAT
GTATTATGCACCAAAGGTATCCGCACCACCTGTTCCTCCAGGATGCTGGAAAACTTTGTGCCGCCGTATAATGCCCACGT
GGTGGACAAACTGGCCGAAGAAGGGGCGGTGCTGCTGGGTAAAACCAACATGGACGAGTTTGCCATGGGTTCTTCCACCG
AAAACTCGGCCTTCTTTACCACCCATAATCCCTGGAATACCGCCAAAGTTCCCGGCGGCTCTTCCGGCGGTTCGGCTGCC
TGTGTGGCTGCTTCGGAAGCAGTCTTTTCACTGGGTTCGGATACCGGCGGTTCTATCCGCCAGCCGGCCAGCTTCTGCAG
TGTTACCGGACTGAAACCCAGCTACGGTATGGTAAGCCGCTACGGGCTGGTAGCCTTTGCCAGTTCGCTTGACCAGATAG
GCCCTTTTACCAAAGATGTTCTGGACTGTGCTCTGGTAATGAATGCCATTGCCGGCTTTGATGACCGTGACTCCACCTCC
GTACCCCAGACAGCGCCTGATTTCAGCAGCTGCCTTGACGGGGATATAAAGGGGTTTAAACTGGGTGTACCCAAAGAATA
TTTCAGCCATAATATGCGGGCAGATATAGCCGAGAAGATAAATGACGCCTTGGGAGTACTTTCGGGTCTGGGTGCAAGCG
TAGACCGGGAGGTATCCCTGCCCCATACTCCATACGCACTGGCGGTTTATTACATACTGGCACCCTCCGAGGCTTCGGCC
AATTTGTCACGGTATGACGGGGTAAAATACGGTTATTCTTACAACCAGACCGAAAACATGTGGGAAGCTATGGAAAAGAC
CCGCGCCAAGGGATTCGGTCCGGAAGTAAAACGGCGTATCATGATAGGCACTTACGCCCTTTCAGCCGGCTATTATGACG
CCTGGTATGTCAAAGCCCAGAAAGTGCGCACTCTGATAAGCCAGGAGTTTAACAACGCCTTTGAAAAATATGACGCCCTT
ATAACACCCACCACCCCCAACTTGCCTTTCAGTATCGGTGAAAAGCTGAGTGACCCCTTTGAGATGTATATGTGTGACAC
CTGCACTATCCCCATAAATATTGCCGGATTGCCCGCCATAAGCATACCGGCCGGGTTTGTAGATGGTTTACCAGTGGGTT
TACAAATTATAGGCAAGCCCTTTGCTGACCAGACTATTATGCGCATAGCCCATGCTTTCCAGTGCGCCACCGCCTGGCAC
AAGGAAACTCCCCGGTTATAG

Upstream 100 bases:

>100_bases
AAGAGGATATTATAAAGCCCTCTTATGACCGTGAGGAGATACTTTCAAATGCCCCCAGACGCGAGGGTGATTACGTGCGG
ATACGGGCGGTGATGGAATA

Downstream 100 bases:

>100_bases
AGAGGTGTGGAAAATCCATGATTAAGCAGCCAAGTGATTGGTATCAGGCCAGTATTGAAACTGTTACCAACCTGGAAACG
ACCCTTAGCCCCAAAGAGGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTDLIKLTIAQSHKLLKERKLSSAELTRAHLDRIEKLEPEIKAFMTVCPESALAQAKAADEAIKQGHIRPLTGIPMALKD
VLCTKGIRTTCSSRMLENFVPPYNAHVVDKLAEEGAVLLGKTNMDEFAMGSSTENSAFFTTHNPWNTAKVPGGSSGGSAA
CVAASEAVFSLGSDTGGSIRQPASFCSVTGLKPSYGMVSRYGLVAFASSLDQIGPFTKDVLDCALVMNAIAGFDDRDSTS
VPQTAPDFSSCLDGDIKGFKLGVPKEYFSHNMRADIAEKINDALGVLSGLGASVDREVSLPHTPYALAVYYILAPSEASA
NLSRYDGVKYGYSYNQTENMWEAMEKTRAKGFGPEVKRRIMIGTYALSAGYYDAWYVKAQKVRTLISQEFNNAFEKYDAL
ITPTTPNLPFSIGEKLSDPFEMYMCDTCTIPINIAGLPAISIPAGFVDGLPVGLQIIGKPFADQTIMRIAHAFQCATAWH
KETPRL

Sequences:

>Translated_486_residues
MTDLIKLTIAQSHKLLKERKLSSAELTRAHLDRIEKLEPEIKAFMTVCPESALAQAKAADEAIKQGHIRPLTGIPMALKD
VLCTKGIRTTCSSRMLENFVPPYNAHVVDKLAEEGAVLLGKTNMDEFAMGSSTENSAFFTTHNPWNTAKVPGGSSGGSAA
CVAASEAVFSLGSDTGGSIRQPASFCSVTGLKPSYGMVSRYGLVAFASSLDQIGPFTKDVLDCALVMNAIAGFDDRDSTS
VPQTAPDFSSCLDGDIKGFKLGVPKEYFSHNMRADIAEKINDALGVLSGLGASVDREVSLPHTPYALAVYYILAPSEASA
NLSRYDGVKYGYSYNQTENMWEAMEKTRAKGFGPEVKRRIMIGTYALSAGYYDAWYVKAQKVRTLISQEFNNAFEKYDAL
ITPTTPNLPFSIGEKLSDPFEMYMCDTCTIPINIAGLPAISIPAGFVDGLPVGLQIIGKPFADQTIMRIAHAFQCATAWH
KETPRL
>Mature_485_residues
TDLIKLTIAQSHKLLKERKLSSAELTRAHLDRIEKLEPEIKAFMTVCPESALAQAKAADEAIKQGHIRPLTGIPMALKDV
LCTKGIRTTCSSRMLENFVPPYNAHVVDKLAEEGAVLLGKTNMDEFAMGSSTENSAFFTTHNPWNTAKVPGGSSGGSAAC
VAASEAVFSLGSDTGGSIRQPASFCSVTGLKPSYGMVSRYGLVAFASSLDQIGPFTKDVLDCALVMNAIAGFDDRDSTSV
PQTAPDFSSCLDGDIKGFKLGVPKEYFSHNMRADIAEKINDALGVLSGLGASVDREVSLPHTPYALAVYYILAPSEASAN
LSRYDGVKYGYSYNQTENMWEAMEKTRAKGFGPEVKRRIMIGTYALSAGYYDAWYVKAQKVRTLISQEFNNAFEKYDALI
TPTTPNLPFSIGEKLSDPFEMYMCDTCTIPINIAGLPAISIPAGFVDGLPVGLQIIGKPFADQTIMRIAHAFQCATAWHK
ETPRL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=489, Percent_Identity=39.4683026584867, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI195972892, Length=502, Percent_Identity=24.5019920318725, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI166795287, Length=180, Percent_Identity=35, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=457, Percent_Identity=36.1050328227571, Blast_Score=273, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=242, Percent_Identity=30.1652892561983, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=221, Percent_Identity=30.316742081448, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=159, Percent_Identity=35.2201257861635, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=247, Percent_Identity=28.3400809716599, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=195, Percent_Identity=31.2820512820513, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556278, Length=195, Percent_Identity=31.2820512820513, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=433, Percent_Identity=36.7205542725173, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6319685, Length=469, Percent_Identity=27.9317697228145, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6320448, Length=233, Percent_Identity=29.6137339055794, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648113, Length=449, Percent_Identity=40.9799554565702, Blast_Score=311, Evalue=8e-85,
Organism=Drosophila melanogaster, GI24644968, Length=515, Percent_Identity=25.4368932038835, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652985, Length=515, Percent_Identity=24.6601941747573, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922090, Length=515, Percent_Identity=24.6601941747573, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24652981, Length=515, Percent_Identity=24.6601941747573, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24652983, Length=515, Percent_Identity=24.6601941747573, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078093, Length=522, Percent_Identity=26.4367816091954, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI45550774, Length=209, Percent_Identity=31.1004784688995, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24648435, Length=209, Percent_Identity=31.1004784688995, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648437, Length=209, Percent_Identity=31.1004784688995, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI21356731, Length=194, Percent_Identity=30.4123711340206, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648441, Length=135, Percent_Identity=34.0740740740741, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648439, Length=135, Percent_Identity=34.0740740740741, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_DEHE1 (Q3Z6V3)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_182046.1
- ProteinModelPortal:   Q3Z6V3
- SMR:   Q3Z6V3
- STRING:   Q3Z6V3
- GeneID:   3229402
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET1335
- NMPDR:   fig|243164.3.peg.1290
- TIGR:   DET1335
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q3Z6V3
- ProtClustDB:   PRK00012
- BioCyc:   DETH243164:DET_1335-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52630; Mature: 52498

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 79-79 ACT_SITE 154-154 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLIKLTIAQSHKLLKERKLSSAELTRAHLDRIEKLEPEIKAFMTVCPESALAQAKAAD
CCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHH
EAIKQGHIRPLTGIPMALKDVLCTKGIRTTCSSRMLENFVPPYNAHVVDKLAEEGAVLLG
HHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEE
KTNMDEFAMGSSTENSAFFTTHNPWNTAKVPGGSSGGSAACVAASEAVFSLGSDTGGSIR
CCCCHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCC
QPASFCSVTGLKPSYGMVSRYGLVAFASSLDQIGPFTKDVLDCALVMNAIAGFDDRDSTS
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCC
VPQTAPDFSSCLDGDIKGFKLGVPKEYFSHNMRADIAEKINDALGVLSGLGASVDREVSL
CCCCCCCHHHHHCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PHTPYALAVYYILAPSEASANLSRYDGVKYGYSYNQTENMWEAMEKTRAKGFGPEVKRRI
CCCCCEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHEE
MIGTYALSAGYYDAWYVKAQKVRTLISQEFNNAFEKYDALITPTTPNLPFSIGEKLSDPF
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHCCCCH
EMYMCDTCTIPINIAGLPAISIPAGFVDGLPVGLQIIGKPFADQTIMRIAHAFQCATAWH
HEEEECCEEEEEEECCCCCCCCCCHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHH
KETPRL
CCCCCC
>Mature Secondary Structure 
TDLIKLTIAQSHKLLKERKLSSAELTRAHLDRIEKLEPEIKAFMTVCPESALAQAKAAD
CCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHH
EAIKQGHIRPLTGIPMALKDVLCTKGIRTTCSSRMLENFVPPYNAHVVDKLAEEGAVLLG
HHHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEE
KTNMDEFAMGSSTENSAFFTTHNPWNTAKVPGGSSGGSAACVAASEAVFSLGSDTGGSIR
CCCCHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCC
QPASFCSVTGLKPSYGMVSRYGLVAFASSLDQIGPFTKDVLDCALVMNAIAGFDDRDSTS
CCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCC
VPQTAPDFSSCLDGDIKGFKLGVPKEYFSHNMRADIAEKINDALGVLSGLGASVDREVSL
CCCCCCCHHHHHCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PHTPYALAVYYILAPSEASANLSRYDGVKYGYSYNQTENMWEAMEKTRAKGFGPEVKRRI
CCCCCEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHEE
MIGTYALSAGYYDAWYVKAQKVRTLISQEFNNAFEKYDALITPTTPNLPFSIGEKLSDPF
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHCCCCH
EMYMCDTCTIPINIAGLPAISIPAGFVDGLPVGLQIIGKPFADQTIMRIAHAFQCATAWH
HEEEECCEEEEEEECCCCCCCCCCHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHH
KETPRL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA