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The map label for this gene is purH [H]

Identifier: 57233807

GI number: 57233807

Start: 1287504

End: 1289045

Strand: Direct

Name: purH [H]

Synonym: DET1417

Alternate gene names: 57233807

Gene position: 1287504-1289045 (Clockwise)

Preceding gene: 57233808

Following gene: 57233798

Centisome position: 87.6

GC content: 53.96

Gene sequence:

>1542_bases
ATGAGGGCTATCCTGAGCGTCTCAGATAAAACCGGTCTTATCGAATTCGCCAAAGGCTTGTCAGAACTGGGTTTTGATAT
ATACAGCACCGGCGGAACCAAGAAATCACTCCAGCAGGCAAATGTAACCGTTCACGGCATTTCGGACATGACCGGTTCGC
CTGAAATACTGGACGGACGGGTCAAAACTTTGCATCCCAAGGTACACGGCGGCATACTGGCCCGGCGTGACCTGCCCGAA
CATATGGCCGAACTGGAAGAACACCATATCCAACCCATTGACATGGTGGTAGTCAATCTCTACCCCTTTGTCAAGACTGT
TTCCCGGCCGGATGTAAGCCTGACTGATGCACTGGAGAATATTGATATCGGCGGACCTACCATGATACGGGCTTCCGCCA
AGAACTTCCCCAGTGTGATTGTGGTGGTAGACCCTCAGGATTACTCCCGTGTACTGGAACACCTTCAGGCAGGCACTCTG
AGCCTTGACGAACGCAAGAAACTGGCCCAAAAGGCCTTCCAGCACGTAGCCATGTATGATACGGCCATCTCCCAGTACCT
CTGGCAGGGAGAAGAGGGTTTCCCCGAAAATATGACCATAGCCCTTTCCAAACGCTATGACCTGCGTTACGGTGAAAACC
CCCACCAGCCGGCTGTTTTCTATGCTGAAAACAGGGTTGGACAAGGGCAGGACAGCGGCATTACCTGGGCGCAGCAGGTC
TGGGGCAAACAGCTTTCCTTTAACAATATTCTGGACGCAGACGCCGCCTGGGGAGCCGCCACTGACTTTGCGGCTGCCAC
AGTAGCCATAGTCAAGCATACCAATACCTGCGGTCTGGCCAGTGACGAAAACATTGCCGAAGCCTACAAGAAGGCCTTTT
CGGGTGACCCCGTTTCGGCTTACGGCGGTATAGTAGCCTCCAACCGCAAAGTGACACTGTCCATGGCCGAAGCCATGAAG
GGTGTCTTTTATGAAATCATCATTGCCCCCGAATACGAACCGGAGGCACTGGAATTCCTTAAAACCCGCAAGGATTTGCG
TATACTCATAGCCGAACTGCCCAAACATGCGGAAAACAAGGCCGCTTCGCTGGATTACCGCCGGGTAAAAGGCGGGCTGC
TGGTGCAGGCGGCTGATGAACTGGCCGAAGAGGCCCTTCAGACCAAGGTAGCCACCAACCGGGCACCCACCGCTGAGGAA
ATGGCAGATTTGAAATTCGCCTGGCGGGCAGTCAAGCATATTAAATCAAACGCCATTGTCCTGGCTAAAAATAAAGTCCT
GCTGGGAATGGGCGCAGGGCAACCCAACCGAGTAGTCAGCGTAGACATTGCCAAGAGCAAGGCCGGTGAGGCCTCAAAGG
GCAGTGTCATGGCCTCAGATGCCATGTTTCCCTTCCCTGACAGCGTTGAACAGGCGGCTGCCGCCGGAGTAACCGCCATT
ATCCAGCCGGGCGGTTCTATCCGTGACCAGGAATCTATTGACGCTGCCAACAAGTACAATATAGCTATGGTATTTACCGG
TACCCGCCACTTCCGCCATTAG

Upstream 100 bases:

>100_bases
TTTCACAGACTATACGGGCATAGCTGCCAAGAGCAGCTTGCCGCAAATATATTTATGCACCTGGTAAAAATAAATTTATT
TAACATAAGGAGGGATTGTC

Downstream 100 bases:

>100_bases
AAAGAACCGGAGGAATTATGCCTGTTAAACCGAAGTTCAATATACCCGGGAACCTGCTCTCAGGTGATACCACTGATATT
TATTTTGCCCGTACGGTGGA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MRAILSVSDKTGLIEFAKGLSELGFDIYSTGGTKKSLQQANVTVHGISDMTGSPEILDGRVKTLHPKVHGGILARRDLPE
HMAELEEHHIQPIDMVVVNLYPFVKTVSRPDVSLTDALENIDIGGPTMIRASAKNFPSVIVVVDPQDYSRVLEHLQAGTL
SLDERKKLAQKAFQHVAMYDTAISQYLWQGEEGFPENMTIALSKRYDLRYGENPHQPAVFYAENRVGQGQDSGITWAQQV
WGKQLSFNNILDADAAWGAATDFAAATVAIVKHTNTCGLASDENIAEAYKKAFSGDPVSAYGGIVASNRKVTLSMAEAMK
GVFYEIIIAPEYEPEALEFLKTRKDLRILIAELPKHAENKAASLDYRRVKGGLLVQAADELAEEALQTKVATNRAPTAEE
MADLKFAWRAVKHIKSNAIVLAKNKVLLGMGAGQPNRVVSVDIAKSKAGEASKGSVMASDAMFPFPDSVEQAAAAGVTAI
IQPGGSIRDQESIDAANKYNIAMVFTGTRHFRH

Sequences:

>Translated_513_residues
MRAILSVSDKTGLIEFAKGLSELGFDIYSTGGTKKSLQQANVTVHGISDMTGSPEILDGRVKTLHPKVHGGILARRDLPE
HMAELEEHHIQPIDMVVVNLYPFVKTVSRPDVSLTDALENIDIGGPTMIRASAKNFPSVIVVVDPQDYSRVLEHLQAGTL
SLDERKKLAQKAFQHVAMYDTAISQYLWQGEEGFPENMTIALSKRYDLRYGENPHQPAVFYAENRVGQGQDSGITWAQQV
WGKQLSFNNILDADAAWGAATDFAAATVAIVKHTNTCGLASDENIAEAYKKAFSGDPVSAYGGIVASNRKVTLSMAEAMK
GVFYEIIIAPEYEPEALEFLKTRKDLRILIAELPKHAENKAASLDYRRVKGGLLVQAADELAEEALQTKVATNRAPTAEE
MADLKFAWRAVKHIKSNAIVLAKNKVLLGMGAGQPNRVVSVDIAKSKAGEASKGSVMASDAMFPFPDSVEQAAAAGVTAI
IQPGGSIRDQESIDAANKYNIAMVFTGTRHFRH
>Mature_513_residues
MRAILSVSDKTGLIEFAKGLSELGFDIYSTGGTKKSLQQANVTVHGISDMTGSPEILDGRVKTLHPKVHGGILARRDLPE
HMAELEEHHIQPIDMVVVNLYPFVKTVSRPDVSLTDALENIDIGGPTMIRASAKNFPSVIVVVDPQDYSRVLEHLQAGTL
SLDERKKLAQKAFQHVAMYDTAISQYLWQGEEGFPENMTIALSKRYDLRYGENPHQPAVFYAENRVGQGQDSGITWAQQV
WGKQLSFNNILDADAAWGAATDFAAATVAIVKHTNTCGLASDENIAEAYKKAFSGDPVSAYGGIVASNRKVTLSMAEAMK
GVFYEIIIAPEYEPEALEFLKTRKDLRILIAELPKHAENKAASLDYRRVKGGLLVQAADELAEEALQTKVATNRAPTAEE
MADLKFAWRAVKHIKSNAIVLAKNKVLLGMGAGQPNRVVSVDIAKSKAGEASKGSVMASDAMFPFPDSVEQAAAAGVTAI
IQPGGSIRDQESIDAANKYNIAMVFTGTRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=476, Percent_Identity=36.9747899159664, Blast_Score=248, Evalue=7e-66,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=48.9603024574669, Blast_Score=479, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI71985564, Length=486, Percent_Identity=33.3333333333333, Blast_Score=236, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI71985574, Length=319, Percent_Identity=24.4514106583072, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323768, Length=475, Percent_Identity=36.8421052631579, Blast_Score=255, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6323056, Length=478, Percent_Identity=36.4016736401674, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24649832, Length=470, Percent_Identity=37.6595744680851, Blast_Score=260, Evalue=2e-69,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55775; Mature: 55775

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAILSVSDKTGLIEFAKGLSELGFDIYSTGGTKKSLQQANVTVHGISDMTGSPEILDGR
CCCEEECCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCEEEEECCCCCCCCHHCCCC
VKTLHPKVHGGILARRDLPEHMAELEEHHIQPIDMVVVNLYPFVKTVSRPDVSLTDALEN
HHEECCHHHCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
IDIGGPTMIRASAKNFPSVIVVVDPQDYSRVLEHLQAGTLSLDERKKLAQKAFQHVAMYD
CCCCCCCEEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
TAISQYLWQGEEGFPENMTIALSKRYDLRYGENPHQPAVFYAENRVGQGQDSGITWAQQV
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHH
WGKQLSFNNILDADAAWGAATDFAAATVAIVKHTNTCGLASDENIAEAYKKAFSGDPVSA
CCCCCCCCCCCCCCCCCCCHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCHH
YGGIVASNRKVTLSMAEAMKGVFYEIIIAPEYEPEALEFLKTRKDLRILIAELPKHAENK
HCCEEECCCEEEEEHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCC
AASLDYRRVKGGLLVQAADELAEEALQTKVATNRAPTAEEMADLKFAWRAVKHIKSNAIV
HHCCCHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
LAKNKVLLGMGAGQPNRVVSVDIAKSKAGEASKGSVMASDAMFPFPDSVEQAAAAGVTAI
EEECEEEEEECCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHCCEEEE
IQPGGSIRDQESIDAANKYNIAMVFTGTRHFRH
ECCCCCCCCCHHCCCCCCEEEEEEEECCHHCCC
>Mature Secondary Structure
MRAILSVSDKTGLIEFAKGLSELGFDIYSTGGTKKSLQQANVTVHGISDMTGSPEILDGR
CCCEEECCCCCCHHHHHHHHHHHCCEEEECCCCHHHHHHCCEEEEECCCCCCCCHHCCCC
VKTLHPKVHGGILARRDLPEHMAELEEHHIQPIDMVVVNLYPFVKTVSRPDVSLTDALEN
HHEECCHHHCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
IDIGGPTMIRASAKNFPSVIVVVDPQDYSRVLEHLQAGTLSLDERKKLAQKAFQHVAMYD
CCCCCCCEEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
TAISQYLWQGEEGFPENMTIALSKRYDLRYGENPHQPAVFYAENRVGQGQDSGITWAQQV
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHH
WGKQLSFNNILDADAAWGAATDFAAATVAIVKHTNTCGLASDENIAEAYKKAFSGDPVSA
CCCCCCCCCCCCCCCCCCCHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCHH
YGGIVASNRKVTLSMAEAMKGVFYEIIIAPEYEPEALEFLKTRKDLRILIAELPKHAENK
HCCEEECCCEEEEEHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCC
AASLDYRRVKGGLLVQAADELAEEALQTKVATNRAPTAEEMADLKFAWRAVKHIKSNAIV
HHCCCHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEE
LAKNKVLLGMGAGQPNRVVSVDIAKSKAGEASKGSVMASDAMFPFPDSVEQAAAAGVTAI
EEECEEEEEECCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHCCEEEE
IQPGGSIRDQESIDAANKYNIAMVFTGTRHFRH
ECCCCCCCCCHHCCCCCCEEEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA