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The map label for this gene is smpB

Identifier: 57233786

GI number: 57233786

Start: 1361164

End: 1361682

Strand: Direct

Name: smpB

Synonym: DET1506

Alternate gene names: 57233786

Gene position: 1361164-1361682 (Clockwise)

Preceding gene: 57233799

Following gene: 57233785

Centisome position: 92.61

GC content: 48.94

Gene sequence:

>519_bases
ATGCATTATGCCCGGATTGTAAGCAATCTGCATCTGGGGTATGATATAATACATATGGCAGAATACGTAACCCTGACAAC
TAACCGCAAGGCATTTCACAACTATTTCCTGGAAGAAAAATACGAAGCCGGGATAATGCTGCTGGGCACGGAGATAAAAT
CCCTCCGAAGCGGCAGAGTAAATATGGGAGATGCCTATGTAAAGCCCCAGAGGGGCGAACTCTGGCTGGTAAATGCCCAT
ATTTCAGCCTACGAGTGCAGCGGACATACCAGCCATGAACCAATGCGGGAACGCAAACTGCTGATGCACCGCAAGGAAAT
AGCCCTGCTCATGTCAAAGGTCAAAGAAAAAGGGCTGACACTGATACCGGTTCGTATTTACCTAAAAAACGATATCGCCA
AGGTAGAGCTGTCACTGGGGCGGGGTAAGAAGCTCTATGACAAAAGGGATACCATAACCAAGCGGGACACCGAACGCGAA
CTTGAGCGGGAGGTCAAATACCGCAATTTCCGCCGCTAG

Upstream 100 bases:

>100_bases
ATATTCCCGGGTAGAGAACTAGTCATCTCTCTGCTCCCTTGTTATCAGTAATGATTACTGTTATTAGTATATGACAGGGG
ATACTCTCTGTCAAGCGCCA

Downstream 100 bases:

>100_bases
TTACCAATTTTTATGGGGACGCGTGGTTTCGACAGGGAAGGTAGGCTACTGGATTGCAGGCTGAGGTGCCATCCACCTCA
TTAAACAGGTGGCAAAAAAC

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MHYARIVSNLHLGYDIIHMAEYVTLTTNRKAFHNYFLEEKYEAGIMLLGTEIKSLRSGRVNMGDAYVKPQRGELWLVNAH
ISAYECSGHTSHEPMRERKLLMHRKEIALLMSKVKEKGLTLIPVRIYLKNDIAKVELSLGRGKKLYDKRDTITKRDTERE
LEREVKYRNFRR

Sequences:

>Translated_172_residues
MHYARIVSNLHLGYDIIHMAEYVTLTTNRKAFHNYFLEEKYEAGIMLLGTEIKSLRSGRVNMGDAYVKPQRGELWLVNAH
ISAYECSGHTSHEPMRERKLLMHRKEIALLMSKVKEKGLTLIPVRIYLKNDIAKVELSLGRGKKLYDKRDTITKRDTERE
LEREVKYRNFRR
>Mature_172_residues
MHYARIVSNLHLGYDIIHMAEYVTLTTNRKAFHNYFLEEKYEAGIMLLGTEIKSLRSGRVNMGDAYVKPQRGELWLVNAH
ISAYECSGHTSHEPMRERKLLMHRKEIALLMSKVKEKGLTLIPVRIYLKNDIAKVELSLGRGKKLYDKRDTITKRDTERE
LEREVKYRNFRR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=150, Percent_Identity=38, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_DEHE1 (Q3Z6E3)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_182206.1
- ProteinModelPortal:   Q3Z6E3
- SMR:   Q3Z6E3
- STRING:   Q3Z6E3
- GeneID:   3229249
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET1506
- NMPDR:   fig|243164.3.peg.1431
- TIGR:   DET1506
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   DETH243164:DET_1506-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 20294; Mature: 20294

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYARIVSNLHLGYDIIHMAEYVTLTTNRKAFHNYFLEEKYEAGIMLLGTEIKSLRSGRV
CCHHHHHHHHCCCHHHHHHHHEEEEECCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCC
NMGDAYVKPQRGELWLVNAHISAYECSGHTSHEPMRERKLLMHRKEIALLMSKVKEKGLT
CCCCEEECCCCCCEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
LIPVRIYLKNDIAKVELSLGRGKKLYDKRDTITKRDTERELEREVKYRNFRR
EEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHYARIVSNLHLGYDIIHMAEYVTLTTNRKAFHNYFLEEKYEAGIMLLGTEIKSLRSGRV
CCHHHHHHHHCCCHHHHHHHHEEEEECCCHHHHHHHHHHHCCCCEEEEECHHHHHHCCCC
NMGDAYVKPQRGELWLVNAHISAYECSGHTSHEPMRERKLLMHRKEIALLMSKVKEKGLT
CCCCEEECCCCCCEEEEECCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCE
LIPVRIYLKNDIAKVELSLGRGKKLYDKRDTITKRDTERELEREVKYRNFRR
EEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA