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The map label for this gene is ogt [H]

Identifier: 57233730

GI number: 57233730

Start: 1317526

End: 1318086

Strand: Direct

Name: ogt [H]

Synonym: DET1460

Alternate gene names: 57233730

Gene position: 1317526-1318086 (Clockwise)

Preceding gene: 57233731

Following gene: 57233854

Centisome position: 89.64

GC content: 54.9

Gene sequence:

>561_bases
TTGAGTAGAGTTACACCAAACGGCCCAAAACCCGTCTCCAGCAGGGTTAAGGCTGCCGCCGGTATAAGCTGGGGTATTCT
TGAAATCCAACCGGGTTGGGTTGGGGTAATGGCATCTGCACAGGGAATCTGCCGCCTTACCCTGCCCCAGCCCGGTCAAA
CCGAAGCACTGGCAGAGCTGAACCCGCCGGCGGAGACTATCCGTACCGACAGCCTTTTCCACCCCCTAGCAACGAAACTT
GAAAGCTATTTTCAGGGAAACAGCACTGATTTCCCGTTCAGCCTTGACCTTAGCGGGTACACCCCGTTTGAATCACAGGT
TTTTAAGACCTGCGCCCTTATACCCTACGGTCAAACTGCCAGTTACGGTCAGATAGCCCGCCGGATAGGCCGCCCCAAAG
CCGCCAGAGCGGTAGGGCAGGCTCTCCATAAAAACCCCGTACCTATACTGATACCCTGCCACAGGGTGATTGCCGCAAAC
GGCAAAACAGGCGGTTTTAACGGGGGCACCAATATAAAATGCTGGCTGCTTACCCTGGAGACTAGGAAACCTTCAGGTTG
A

Upstream 100 bases:

>100_bases
GCTGGTTGAACCCGTACCGGAGTAGTTTTTGATGTCCGGCCTTCCTCCTCAACATACCCAGGAGACCGCAGGCTCAAAAA
TCAGGGGTGGATAACCGCTT

Downstream 100 bases:

>100_bases
TAACCAGCGTATCAGCCGGTACGGAATCCCACGGGCGGGAATAATAAAACCAAAGCTCGGTCTCATCTGTATCCAAGGCC
CTCAGACGGAAATATTCGGT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MSRVTPNGPKPVSSRVKAAAGISWGILEIQPGWVGVMASAQGICRLTLPQPGQTEALAELNPPAETIRTDSLFHPLATKL
ESYFQGNSTDFPFSLDLSGYTPFESQVFKTCALIPYGQTASYGQIARRIGRPKAARAVGQALHKNPVPILIPCHRVIAAN
GKTGGFNGGTNIKCWLLTLETRKPSG

Sequences:

>Translated_186_residues
MSRVTPNGPKPVSSRVKAAAGISWGILEIQPGWVGVMASAQGICRLTLPQPGQTEALAELNPPAETIRTDSLFHPLATKL
ESYFQGNSTDFPFSLDLSGYTPFESQVFKTCALIPYGQTASYGQIARRIGRPKAARAVGQALHKNPVPILIPCHRVIAAN
GKTGGFNGGTNIKCWLLTLETRKPSG
>Mature_185_residues
SRVTPNGPKPVSSRVKAAAGISWGILEIQPGWVGVMASAQGICRLTLPQPGQTEALAELNPPAETIRTDSLFHPLATKLE
SYFQGNSTDFPFSLDLSGYTPFESQVFKTCALIPYGQTASYGQIARRIGRPKAARAVGQALHKNPVPILIPCHRVIAANG
KTGGFNGGTNIKCWLLTLETRKPSG

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=150, Percent_Identity=34.6666666666667, Blast_Score=85, Evalue=5e-17,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=48.5714285714286, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1788542, Length=162, Percent_Identity=32.7160493827161, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=43.9024390243902, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI115533068, Length=82, Percent_Identity=43.9024390243902, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320001, Length=80, Percent_Identity=38.75, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17137554, Length=123, Percent_Identity=36.5853658536585, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19885; Mature: 19754

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVTPNGPKPVSSRVKAAAGISWGILEIQPGWVGVMASAQGICRLTLPQPGQTEALAEL
CCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCEEEEEECCCCEEEEECCCCCCCCHHHHC
NPPAETIRTDSLFHPLATKLESYFQGNSTDFPFSLDLSGYTPFESQVFKTCALIPYGQTA
CCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCC
SYGQIARRIGRPKAARAVGQALHKNPVPILIPCHRVIAANGKTGGFNGGTNIKCWLLTLE
CHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCCCCCCEEEEEEEEE
TRKPSG
CCCCCC
>Mature Secondary Structure 
SRVTPNGPKPVSSRVKAAAGISWGILEIQPGWVGVMASAQGICRLTLPQPGQTEALAEL
CCCCCCCCCCHHHHHHHHCCCEEEEEEECCCEEEEEECCCCEEEEECCCCCCCCHHHHC
NPPAETIRTDSLFHPLATKLESYFQGNSTDFPFSLDLSGYTPFESQVFKTCALIPYGQTA
CCCHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCC
SYGQIARRIGRPKAARAVGQALHKNPVPILIPCHRVIAANGKTGGFNGGTNIKCWLLTLE
CHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCCCCCCEEEEEEEEE
TRKPSG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]