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The map label for this gene is pit [H]

Identifier: 57233623

GI number: 57233623

Start: 30503

End: 31504

Strand: Direct

Name: pit [H]

Synonym: DET0034

Alternate gene names: 57233623

Gene position: 30503-31504 (Clockwise)

Preceding gene: 57233624

Following gene: 57233613

Centisome position: 2.08

GC content: 53.59

Gene sequence:

>1002_bases
ATGCCTGATGCCTCGTTTATCCCTTATCTGATTATTATTCTTTTAGCTATAGGTTTTGCCTTTGTAAACGGTACCAATGA
CACGGCCAATGCCATTGCCACGGTGGTGGGTACGCGGGTTTTGTCTCCCCGCAAGGCTATAATCATGGCGGCAGCGGCTA
ACCTGGTGGGTGTTTTTACCGGCACGGCTGTTGCCCGGACTATCGGCAAAGGCATACTGGGGGCTGAATCACTTACCTAT
GAAACAGTTATTGCCGGGCTGATAGCCGCGGTTATTTGGTCTATGGTGGCTACCCGTAAGGGTCTGCCGGTCAGCCTGAC
ACACTGTCTGGTGGCGGGCTTAGCTATGGCCGGAGTAGCTATGGCCGGGGTTGGGGCAGTAGACTGGGGAGTTCTTTTAA
AGATTGTGGCGGCGGTAGGTATTGCCCCCATTATGGGCATGGCGGGCGGTTATCTGCTTATGGTTGCCCTGCTCTGGATA
TTTCGGCGGAGTGTTCCTTCCAAGACTCACGGCCTTTTTTCCCACCTGCAGATATTTTCAGCCGCTTTTATGGCTTTCAG
CCACGGGCGGAGTGACGGGCAGATGCCTATGGGTATTATTGCTCTGGGCACAGTCCTTTATACCGGCAATGCCGGTCTCT
GGGATGCTATACCCATCTGGATTATTATACTTTCGGCTTTGTCCATCAGCGCCGGGACGGCTATTGGCGGCTGGAGGGTT
ATTCAGACAGTGGGTGTGCGAATGACCAATTTGCGGCCTATAAACGGTTTTGCGGCTGAAACAGCGGCTGCCACGGTTAT
CCAGATAGCCGCTTCCATCGGTATACCTGTTTCCAGCACCCATGCCATTTCTTCGGCCATTATGGGGGTGGGGGCTACCC
GCAGGCTCTCGGCGGTGCGCTGGGGCGTGGCAGGCAATATTGTGAGTGCCTGGCTGCTTACCTTTCCCATTTGCGGTGCT
TTAGGTTTTCTGGTAGCCGGTTTGTTTAAGCTTATATTCTAA

Upstream 100 bases:

>100_bases
GCAAACATTATCAAGTGGCGTGAAATATATGAACATATGGAAACGGCCACAGACCGGTGCGAAGACGTGGCCAATGTTCT
TGAGGGGGTTGCCCTGAAGA

Downstream 100 bases:

>100_bases
AGTTCAATAGAACGCGGGTTTATGCGGCAGTGTTCAGCCGAGATTATAGACATAATCTCGCTGACTGCATTATCAATCTC
CCCCTCAGGGTTGACTACCA

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MPDASFIPYLIIILLAIGFAFVNGTNDTANAIATVVGTRVLSPRKAIIMAAAANLVGVFTGTAVARTIGKGILGAESLTY
ETVIAGLIAAVIWSMVATRKGLPVSLTHCLVAGLAMAGVAMAGVGAVDWGVLLKIVAAVGIAPIMGMAGGYLLMVALLWI
FRRSVPSKTHGLFSHLQIFSAAFMAFSHGRSDGQMPMGIIALGTVLYTGNAGLWDAIPIWIIILSALSISAGTAIGGWRV
IQTVGVRMTNLRPINGFAAETAAATVIQIAASIGIPVSSTHAISSAIMGVGATRRLSAVRWGVAGNIVSAWLLTFPICGA
LGFLVAGLFKLIF

Sequences:

>Translated_333_residues
MPDASFIPYLIIILLAIGFAFVNGTNDTANAIATVVGTRVLSPRKAIIMAAAANLVGVFTGTAVARTIGKGILGAESLTY
ETVIAGLIAAVIWSMVATRKGLPVSLTHCLVAGLAMAGVAMAGVGAVDWGVLLKIVAAVGIAPIMGMAGGYLLMVALLWI
FRRSVPSKTHGLFSHLQIFSAAFMAFSHGRSDGQMPMGIIALGTVLYTGNAGLWDAIPIWIIILSALSISAGTAIGGWRV
IQTVGVRMTNLRPINGFAAETAAATVIQIAASIGIPVSSTHAISSAIMGVGATRRLSAVRWGVAGNIVSAWLLTFPICGA
LGFLVAGLFKLIF
>Mature_332_residues
PDASFIPYLIIILLAIGFAFVNGTNDTANAIATVVGTRVLSPRKAIIMAAAANLVGVFTGTAVARTIGKGILGAESLTYE
TVIAGLIAAVIWSMVATRKGLPVSLTHCLVAGLAMAGVAMAGVGAVDWGVLLKIVAAVGIAPIMGMAGGYLLMVALLWIF
RRSVPSKTHGLFSHLQIFSAAFMAFSHGRSDGQMPMGIIALGTVLYTGNAGLWDAIPIWIIILSALSISAGTAIGGWRVI
QTVGVRMTNLRPINGFAAETAAATVIQIAASIGIPVSSTHAISSAIMGVGATRRLSAVRWGVAGNIVSAWLLTFPICGAL
GFLVAGLFKLIF

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=162, Percent_Identity=37.037037037037, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI5803173, Length=151, Percent_Identity=37.0860927152318, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI1789907, Length=214, Percent_Identity=29.9065420560748, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789360, Length=215, Percent_Identity=28.8372093023256, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25146401, Length=158, Percent_Identity=33.5443037974684, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71981576, Length=149, Percent_Identity=30.8724832214765, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17539280, Length=167, Percent_Identity=33.5329341317365, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32566716, Length=165, Percent_Identity=28.4848484848485, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536725, Length=171, Percent_Identity=30.4093567251462, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17557328, Length=169, Percent_Identity=31.3609467455621, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319773, Length=173, Percent_Identity=28.3236994219653, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21356511, Length=176, Percent_Identity=31.25, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 34227; Mature: 34096

Theoretical pI: Translated: 11.08; Mature: 11.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
PDASFIPYLIIILLAIGFAFVNGTNDTANAIATVVGTRVLSPRKAIIMAAAANLVGVFT
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]