Click here to switch to the map view.

The map label for this gene is holA [C]

Identifier: 57233595

GI number: 57233595

Start: 38985

End: 40184

Strand: Direct

Name: holA [C]

Synonym: DET0047

Alternate gene names: 57233595

Gene position: 38985-40184 (Clockwise)

Preceding gene: 57233597

Following gene: 57233594

Centisome position: 2.65

GC content: 51.42

Gene sequence:

>1200_bases
ATGTTTGCGGAGTTCGTAGGGCATTACAACTTCTTCGGGCATTGTTTCAAATCCTCCAAGTGCAGCTTACGGGCAGATTG
TAACATACTGCCAGGCATTTTTGCACTAAACTTCAAAGGTTACGCGCCCGTTTCGTTATGCTACAATATCAGCCGAAGGT
GGCTGTTAAATTTGCTGTATATTTTTACCGGCAGTGATGATTTTTCCCTGAAAGAACGCCTGAAGCAGATAAAACTGGCT
TTGGGTGATGAGTCTTTGTCCTCTGCCAATACCACCATGCTGGAGGGCAAAAACCTGACGGCTGCCGAACTTCAAAACTA
TGTCCAGACTATTCCCTTTTTAGCCCCTGCCCGGCTGGTTATGGTAAACGGCCTGCTGGAGAGGTTTGAAGGCTCAAAAG
GTAAAGCCGCCGCCAAAGATAAGGCAGACAAAAACAGCCCGGATGAATTTGCCCGGGCGCTGTCTAATCTGCCTCCCACA
ACCCTGGCGGTGCTTCTGGACTATACCACCGCTAAAGACGGTGATTACCGTGAAAAAGAAAAAGTATCCGCTTTGGCCTC
AAACCCCCTTTTTAAGCTGGTAAGCCCCGGTGCTGAGGTAAAGCACTTTGCACCCCTTTCTGCCCAGAACCTGCCCGGCT
GGGTAATGCAGCGTGCCAAACAGTCTTCTATCCAGATGTCTGTTCCGGCAGCCCGTCTGCTGGCCCGTTTTGTGGGGGCG
GACTTGTGGGCGCTTTCCTCCGAGATTGAGAAACTGGGCTTGTATGCCCAGGGGCGGGCGGTGGAGGAAGCCGATGTTGA
AAATATGGTGGGTTATTCCCAGGAAGTAAATATATTCGGTTTGGTGGATGCCGTACTGGACCGCAAGGTTAAAGACGCCC
AGCAGTCTCTGGCGGCTTTTACTGCCGGCGGCGGGTCACCCGGTTTTTTGCTGTTTATGCTGGTACGCCAGCTGCGGCTG
ATGGTGCGTTATACCGCCCTTAAAAAGCGGGGGCTGAAAGAGGCCGAGATTTTTAAAGCTATGGGCGGCTCTGAGTACGC
CCTTAGGAAAACAGCCTCTCAGGCGGCCTGCCAGAACATGGACAGCCTTAAAGCTATTTATTCCAAACTGCTGGAAACAG
ATTTTAATATAAAAACCGGCCGCTTTGAACCGGAGCTGGCCATGGGTTTGCTGGTTGCCGAACTTTGCGGGAGAAAATAG

Upstream 100 bases:

>100_bases
GCAGGGCGCAGAGAGCCTCGTAGGAACCAAAGCGGGCAATATGTTTAATGGTCTGTTCCCTTATTTCAGGGGCTATTTCC
ATATCATTGGCAATGGCAAT

Downstream 100 bases:

>100_bases
CTTGGAAGAAAAACGAAAACAGTCACTGAAAGAACAGTTGAAAGCCGTAAACTGGGGCAGTCTGCTGCTGACCTTCGGGG
CTCTTATAGCACTCAGCTTT

Product: DNA polymerase III delta subunit-related protein

Products: Pyrophosphate; DNA [C]

Alternate protein names: DNA Polymerase III Subunit Delta; DNA Polymerase III Delta Subunit-Related Protein; DNA Polymerase III Delta Subunit-Like Protein

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MFAEFVGHYNFFGHCFKSSKCSLRADCNILPGIFALNFKGYAPVSLCYNISRRWLLNLLYIFTGSDDFSLKERLKQIKLA
LGDESLSSANTTMLEGKNLTAAELQNYVQTIPFLAPARLVMVNGLLERFEGSKGKAAAKDKADKNSPDEFARALSNLPPT
TLAVLLDYTTAKDGDYREKEKVSALASNPLFKLVSPGAEVKHFAPLSAQNLPGWVMQRAKQSSIQMSVPAARLLARFVGA
DLWALSSEIEKLGLYAQGRAVEEADVENMVGYSQEVNIFGLVDAVLDRKVKDAQQSLAAFTAGGGSPGFLLFMLVRQLRL
MVRYTALKKRGLKEAEIFKAMGGSEYALRKTASQAACQNMDSLKAIYSKLLETDFNIKTGRFEPELAMGLLVAELCGRK

Sequences:

>Translated_399_residues
MFAEFVGHYNFFGHCFKSSKCSLRADCNILPGIFALNFKGYAPVSLCYNISRRWLLNLLYIFTGSDDFSLKERLKQIKLA
LGDESLSSANTTMLEGKNLTAAELQNYVQTIPFLAPARLVMVNGLLERFEGSKGKAAAKDKADKNSPDEFARALSNLPPT
TLAVLLDYTTAKDGDYREKEKVSALASNPLFKLVSPGAEVKHFAPLSAQNLPGWVMQRAKQSSIQMSVPAARLLARFVGA
DLWALSSEIEKLGLYAQGRAVEEADVENMVGYSQEVNIFGLVDAVLDRKVKDAQQSLAAFTAGGGSPGFLLFMLVRQLRL
MVRYTALKKRGLKEAEIFKAMGGSEYALRKTASQAACQNMDSLKAIYSKLLETDFNIKTGRFEPELAMGLLVAELCGRK
>Mature_399_residues
MFAEFVGHYNFFGHCFKSSKCSLRADCNILPGIFALNFKGYAPVSLCYNISRRWLLNLLYIFTGSDDFSLKERLKQIKLA
LGDESLSSANTTMLEGKNLTAAELQNYVQTIPFLAPARLVMVNGLLERFEGSKGKAAAKDKADKNSPDEFARALSNLPPT
TLAVLLDYTTAKDGDYREKEKVSALASNPLFKLVSPGAEVKHFAPLSAQNLPGWVMQRAKQSSIQMSVPAARLLARFVGA
DLWALSSEIEKLGLYAQGRAVEEADVENMVGYSQEVNIFGLVDAVLDRKVKDAQQSLAAFTAGGGSPGFLLFMLVRQLRL
MVRYTALKKRGLKEAEIFKAMGGSEYALRKTASQAACQNMDSLKAIYSKLLETDFNIKTGRFEPELAMGLLVAELCGRK

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Delta Subunit Seems To Interact With The Gamma Subunit To Transfer The B

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 43892; Mature: 43892

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAEFVGHYNFFGHCFKSSKCSLRADCNILPGIFALNFKGYAPVSLCYNISRRWLLNLLY
CCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
IFTGSDDFSLKERLKQIKLALGDESLSSANTTMLEGKNLTAAELQNYVQTIPFLAPARLV
HHCCCCCHHHHHHHHHHHHHHCCCHHCCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHHH
MVNGLLERFEGSKGKAAAKDKADKNSPDEFARALSNLPPTTLAVLLDYTTAKDGDYREKE
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHH
KVSALASNPLFKLVSPGAEVKHFAPLSAQNLPGWVMQRAKQSSIQMSVPAARLLARFVGA
HHHHHHCCCHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCEECCCHHHHHHHHHCC
DLWALSSEIEKLGLYAQGRAVEEADVENMVGYSQEVNIFGLVDAVLDRKVKDAQQSLAAF
HHHHHHHHHHHHCCHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAGGGSPGFLLFMLVRQLRLMVRYTALKKRGLKEAEIFKAMGGSEYALRKTASQAACQNM
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCH
DSLKAIYSKLLETDFNIKTGRFEPELAMGLLVAELCGRK
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFAEFVGHYNFFGHCFKSSKCSLRADCNILPGIFALNFKGYAPVSLCYNISRRWLLNLLY
CCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHH
IFTGSDDFSLKERLKQIKLALGDESLSSANTTMLEGKNLTAAELQNYVQTIPFLAPARLV
HHCCCCCHHHHHHHHHHHHHHCCCHHCCCCCEEECCCCCCHHHHHHHHHHCCCHHHHHHH
MVNGLLERFEGSKGKAAAKDKADKNSPDEFARALSNLPPTTLAVLLDYTTAKDGDYREKE
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHH
KVSALASNPLFKLVSPGAEVKHFAPLSAQNLPGWVMQRAKQSSIQMSVPAARLLARFVGA
HHHHHHCCCHHHHHCCCCCHHHCCCCCCCCCCHHHHHHHHHHCCEECCCHHHHHHHHHCC
DLWALSSEIEKLGLYAQGRAVEEADVENMVGYSQEVNIFGLVDAVLDRKVKDAQQSLAAF
HHHHHHHHHHHHCCHHCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAGGGSPGFLLFMLVRQLRLMVRYTALKKRGLKEAEIFKAMGGSEYALRKTASQAACQNM
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCH
DSLKAIYSKLLETDFNIKTGRFEPELAMGLLVAELCGRK
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: Deoxynucleoside Triphosphate [C]

Specific reaction: (n)Deoxynucleoside Triphosphate = (n)Pyrophosphate + DNA(n) [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10 -Phenanthroline; 2', 3'-Dideoxythymidine 5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA