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The map label for this gene is radA [H]

Identifier: 57233577

GI number: 57233577

Start: 53762

End: 55150

Strand: Direct

Name: radA [H]

Synonym: DET0058

Alternate gene names: 57233577

Gene position: 53762-55150 (Clockwise)

Preceding gene: 57233578

Following gene: 57233576

Centisome position: 3.66

GC content: 52.84

Gene sequence:

>1389_bases
ATGGAAAAAAGCCGTAGAGTTTATGTTTGTTCCAACTGCGGGCGTGAGAGCCTGAAATGGCTGGGCCGCTGCCCCGCCTG
TCAGGAGTGGAATACTTTTGAAGAAAAAACGGTTGTTTCGCCCATAGGGCGTAAAAATGAACCTGCCCGGGTTATCAGCC
CGGCGGCAGAGCTTTCCAGTTTACAGGCATCCGAAACCACCCGGCGGAGTTTATCCATTTCGGAGTTTAACCGGGTTTTA
GGCGGCGGCATAGTGCCGGGTTCGCTTATGCTTTTAGGGGGTGAACCGGGCATCGGCAAATCCACTCTGCTTCTTCAGGT
GGCGGCTTCGGTAGCCCAAAGCGGCGGCAAGGTAGTATATGTATCCGGGGAAGAAAACCCCGCCCAGATAAAAATGCGCG
CCCAACGCCTGGGTATCAGCGGCGAAGGGCTTTTTCTTATGGCTGAGACTGACCTTAATGCCATTTTAGCCCAGCTTTCA
GTCCTCTGCCCGTTACTGGTCGTTATTGACTCTATCCAGACTGTATTTCTGCCGGAGCTTGAAGCCGCACCCGGTGCTAT
AAATCAGGTGCGTGAGTCAGCCCTCCGCCTGATGCAGTGGGCTAAAAGCAGCGGAGCCAGCGTATTTATAACCGCCCATG
TTACCAAAGAGGGAAACATAGCCGGGCCGCGTATACTGGAACATATAGTAGATGTGGTTATGTACTTTGAGGGAGAGTCC
CAGAGTGCTTACCGTTTAGTGCGTTCGGTAAAAAACCGTTTCGGTTCTACCAACGAAGTGGGTATATTTGAAATGAAAAG
TGAGGGTCTGGTAGAAGTAGCCAACCCTTCCCAGATATTTTTATCAAACCGCCAGGCAAATACGGTCGGCTCGGCTGTAA
CCGCTGTGCTGGAGGGCAGCCGCCCGCTGCTGGTTGAAGTGCAGGCACTTACCAATACCACCAGTTTCGGCCAGCCCCGC
CGCACCGCCAACGGGGTGGATTTTAACCGTACCCTTATGATAGCCGCTGTCCTCTCCAAACGCCTGTCCATGCGTTTAGG
TACCCAGGACATAATAGTCAACGCTACCGGCGGTATCCGTCTGGATGAGCCGGCCGCAGATTTAGCTATAGCTCTGGCCA
TTGCTTCCAGTTACCGGGATACCGGCGTCTGCCCAGAAACCATTGCTCTTGGGGAGATTGGTCTTTCTGGTGAACTGCGG
ACTATTCCCCATCTGGAAAGGCGTCTTTCCGAGGCCAGCCGTCTGGGTTTTAAGAGGGCTTTAGTACCGGCCGGTGCTAA
CTGCCACAATGTACATAATATCCAGATAATTCCGGTTTCAACTGTCAAAGAGGCGGTGAAGCTGGCACTTACCGGGGTAA
AAACGGAGACCGAAGATGTTTTTGAATGA

Upstream 100 bases:

>100_bases
GGCGTGTACTTTGAATCATATCTTAGTCGCAACATCTGACGCCGTTTTGGGCATCAGTATATCCTTGCGTTAGCGGATTA
AATTGTGTAGTCTTTGGTAT

Downstream 100 bases:

>100_bases
AAAAGTCGGGGCAGTTATTGTAGCTGCCGGCCAGAGCCGCCGGATGGGCGGGCAGGACAAAATATTTGCCCGGCTTTCGG
GTAAACCTGTTTTGGCTCAC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MEKSRRVYVCSNCGRESLKWLGRCPACQEWNTFEEKTVVSPIGRKNEPARVISPAAELSSLQASETTRRSLSISEFNRVL
GGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVYVSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLS
VLCPLLVVIDSIQTVFLPELEAAPGAINQVRESALRLMQWAKSSGASVFITAHVTKEGNIAGPRILEHIVDVVMYFEGES
QSAYRLVRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQANTVGSAVTAVLEGSRPLLVEVQALTNTTSFGQPR
RTANGVDFNRTLMIAAVLSKRLSMRLGTQDIIVNATGGIRLDEPAADLAIALAIASSYRDTGVCPETIALGEIGLSGELR
TIPHLERRLSEASRLGFKRALVPAGANCHNVHNIQIIPVSTVKEAVKLALTGVKTETEDVFE

Sequences:

>Translated_462_residues
MEKSRRVYVCSNCGRESLKWLGRCPACQEWNTFEEKTVVSPIGRKNEPARVISPAAELSSLQASETTRRSLSISEFNRVL
GGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVYVSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLS
VLCPLLVVIDSIQTVFLPELEAAPGAINQVRESALRLMQWAKSSGASVFITAHVTKEGNIAGPRILEHIVDVVMYFEGES
QSAYRLVRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQANTVGSAVTAVLEGSRPLLVEVQALTNTTSFGQPR
RTANGVDFNRTLMIAAVLSKRLSMRLGTQDIIVNATGGIRLDEPAADLAIALAIASSYRDTGVCPETIALGEIGLSGELR
TIPHLERRLSEASRLGFKRALVPAGANCHNVHNIQIIPVSTVKEAVKLALTGVKTETEDVFE
>Mature_462_residues
MEKSRRVYVCSNCGRESLKWLGRCPACQEWNTFEEKTVVSPIGRKNEPARVISPAAELSSLQASETTRRSLSISEFNRVL
GGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVYVSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLS
VLCPLLVVIDSIQTVFLPELEAAPGAINQVRESALRLMQWAKSSGASVFITAHVTKEGNIAGPRILEHIVDVVMYFEGES
QSAYRLVRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQANTVGSAVTAVLEGSRPLLVEVQALTNTTSFGQPR
RTANGVDFNRTLMIAAVLSKRLSMRLGTQDIIVNATGGIRLDEPAADLAIALAIASSYRDTGVCPETIALGEIGLSGELR
TIPHLERRLSEASRLGFKRALVPAGANCHNVHNIQIIPVSTVKEAVKLALTGVKTETEDVFE

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=427, Percent_Identity=43.559718969555, Blast_Score=377, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49547; Mature: 49547

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKSRRVYVCSNCGRESLKWLGRCPACQEWNTFEEKTVVSPIGRKNEPARVISPAAELSS
CCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCHHCCCHHHHHH
LQASETTRRSLSISEFNRVLGGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVY
HHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEE
VSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLSVLCPLLVVIDSIQTVFLPEL
EECCCCHHHHHEEHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EAAPGAINQVRESALRLMQWAKSSGASVFITAHVTKEGNIAGPRILEHIVDVVMYFEGES
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHEECCCC
QSAYRLVRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQANTVGSAVTAVLEGS
HHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCEEEEECCCCCHHHHHHHHHHCCC
RPLLVEVQALTNTTSFGQPRRTANGVDFNRTLMIAAVLSKRLSMRLGTQDIIVNATGGIR
CCEEEEEEEHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LDEPAADLAIALAIASSYRDTGVCPETIALGEIGLSGELRTIPHLERRLSEASRLGFKRA
ECCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
LVPAGANCHNVHNIQIIPVSTVKEAVKLALTGVKTETEDVFE
HCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCHHHHCC
>Mature Secondary Structure
MEKSRRVYVCSNCGRESLKWLGRCPACQEWNTFEEKTVVSPIGRKNEPARVISPAAELSS
CCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCHHCCCHHHHHH
LQASETTRRSLSISEFNRVLGGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVY
HHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEE
VSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLSVLCPLLVVIDSIQTVFLPEL
EECCCCHHHHHEEHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EAAPGAINQVRESALRLMQWAKSSGASVFITAHVTKEGNIAGPRILEHIVDVVMYFEGES
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHEECCCC
QSAYRLVRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQANTVGSAVTAVLEGS
HHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCCEEEEECCCCCHHHHHHHHHHCCC
RPLLVEVQALTNTTSFGQPRRTANGVDFNRTLMIAAVLSKRLSMRLGTQDIIVNATGGIR
CCEEEEEEEHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
LDEPAADLAIALAIASSYRDTGVCPETIALGEIGLSGELRTIPHLERRLSEASRLGFKRA
ECCCHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHH
LVPAGANCHNVHNIQIIPVSTVKEAVKLALTGVKTETEDVFE
HCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]