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The map label for this gene is ispD

Identifier: 57233576

GI number: 57233576

Start: 55137

End: 55820

Strand: Direct

Name: ispD

Synonym: DET0059

Alternate gene names: 57233576

Gene position: 55137-55820 (Clockwise)

Preceding gene: 57233577

Following gene: 57233568

Centisome position: 3.75

GC content: 51.61

Gene sequence:

>684_bases
ATGTTTTTGAATGAAAAAGTCGGGGCAGTTATTGTAGCTGCCGGCCAGAGCCGCCGGATGGGCGGGCAGGACAAAATATT
TGCCCGGCTTTCGGGTAAACCTGTTTTGGCTCACACCCTTTCGGTTTTTCAGCAGTCCCCGGAGATAGATGATATTGCCC
TGGTGGTATCCGAACACAATCTTAAAAAAGCTAAAGAACTGGTCAAAGAATATAATTTCAGCAAGGTAATTGCTATATGC
TCCGGCGGCGAACTCCGTCAGGACTCCGTTTCTTCAGGGCTGACTGCCTTGTGTGATTGCGGCTGGGTGCTTATTCATGA
CGGGGCGCGCCCTCTGCTTGACCCTGTCTCCATACCCGAAGGGCTGGAAGCGGCCAAACTCTGCGGTTCGGCCGTTGCGG
CCGTACCCCTCAAAGACACCGTCAAGGAAATATCCCCAGAAGGGCTGGTGGAAAAGACCCTGCCTAGGGAAAAACTGATA
TCTGTCCAGACGCCTCAGGTATTTAGGGCAGACATTATCCAGAAAGCCTACCGCAGGGTGGGCATAACCGCCACTGATGA
TGCCCAACTGGTAGAAAAGCTGAAGCTCCCGGTCAAGATATTCTCCGGCGCATATGCTAATATAAAGATAACCACACCCG
AAGACCTGCTTATGGCAGAAATACTGCTGAAGAAAGGACGGTGA

Upstream 100 bases:

>100_bases
GCTAACTGCCACAATGTACATAATATCCAGATAATTCCGGTTTCAACTGTCAAAGAGGCGGTGAAGCTGGCACTTACCGG
GGTAAAAACGGAGACCGAAG

Downstream 100 bases:

>100_bases
GCATAATGCGTATTGGAAACGGTTATGATGTACATCGTCTGGCACCGGGGCAGAAGCTGGTGCTGGGCGGGGTGGAAATA
CCCTTTGAGTGCGGGCTTAT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MFLNEKVGAVIVAAGQSRRMGGQDKIFARLSGKPVLAHTLSVFQQSPEIDDIALVVSEHNLKKAKELVKEYNFSKVIAIC
SGGELRQDSVSSGLTALCDCGWVLIHDGARPLLDPVSIPEGLEAAKLCGSAVAAVPLKDTVKEISPEGLVEKTLPREKLI
SVQTPQVFRADIIQKAYRRVGITATDDAQLVEKLKLPVKIFSGAYANIKITTPEDLLMAEILLKKGR

Sequences:

>Translated_227_residues
MFLNEKVGAVIVAAGQSRRMGGQDKIFARLSGKPVLAHTLSVFQQSPEIDDIALVVSEHNLKKAKELVKEYNFSKVIAIC
SGGELRQDSVSSGLTALCDCGWVLIHDGARPLLDPVSIPEGLEAAKLCGSAVAAVPLKDTVKEISPEGLVEKTLPREKLI
SVQTPQVFRADIIQKAYRRVGITATDDAQLVEKLKLPVKIFSGAYANIKITTPEDLLMAEILLKKGR
>Mature_227_residues
MFLNEKVGAVIVAAGQSRRMGGQDKIFARLSGKPVLAHTLSVFQQSPEIDDIALVVSEHNLKKAKELVKEYNFSKVIAIC
SGGELRQDSVSSGLTALCDCGWVLIHDGARPLLDPVSIPEGLEAAKLCGSAVAAVPLKDTVKEISPEGLVEKTLPREKLI
SVQTPQVFRADIIQKAYRRVGITATDDAQLVEKLKLPVKIFSGAYANIKITTPEDLLMAEILLKKGR

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=236, Percent_Identity=29.2372881355932, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1789104, Length=226, Percent_Identity=31.4159292035398, Blast_Score=100, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_DEHE1 (Q3ZAD7)

Other databases:

- EMBL:   CP000027
- RefSeq:   YP_180812.1
- ProteinModelPortal:   Q3ZAD7
- SMR:   Q3ZAD7
- STRING:   Q3ZAD7
- GeneID:   3229038
- GenomeReviews:   CP000027_GR
- KEGG:   det:DET0059
- NMPDR:   fig|243164.3.peg.69
- TIGR:   DET0059
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   PSNIKVT
- PhylomeDB:   Q3ZAD7
- ProtClustDB:   PRK00155
- BioCyc:   DETH243164:DET_0059-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 24556; Mature: 24556

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLNEKVGAVIVAAGQSRRMGGQDKIFARLSGKPVLAHTLSVFQQSPEIDDIALVVSEHN
CCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCC
LKKAKELVKEYNFSKVIAICSGGELRQDSVSSGLTALCDCGWVLIHDGARPLLDPVSIPE
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCHHHHHHCCEEEEECCCCCCCCCCCCCC
GLEAAKLCGSAVAAVPLKDTVKEISPEGLVEKTLPREKLISVQTPQVFRADIIQKAYRRV
CHHHHHHHHHHHEECCHHHHHHHCCCCCHHHHHCCHHHHEECCCCHHHHHHHHHHHHHHC
GITATDDAQLVEKLKLPVKIFSGAYANIKITTPEDLLMAEILLKKGR
CCCCCCHHHHHHHHCCCHHHHCCCEEEEEEECHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFLNEKVGAVIVAAGQSRRMGGQDKIFARLSGKPVLAHTLSVFQQSPEIDDIALVVSEHN
CCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCC
LKKAKELVKEYNFSKVIAICSGGELRQDSVSSGLTALCDCGWVLIHDGARPLLDPVSIPE
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCHHHHHHCCEEEEECCCCCCCCCCCCCC
GLEAAKLCGSAVAAVPLKDTVKEISPEGLVEKTLPREKLISVQTPQVFRADIIQKAYRRV
CHHHHHHHHHHHEECCHHHHHHHCCCCCHHHHHCCHHHHEECCCCHHHHHHHHHHHHHHC
GITATDDAQLVEKLKLPVKIFSGAYANIKITTPEDLLMAEILLKKGR
CCCCCCHHHHHHHHCCCHHHHCCCEEEEEEECHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA