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The map label for this gene is gidB

Identifier: 38234911

GI number: 38234911

Start: 2485137

End: 2485808

Strand: Reverse

Name: gidB

Synonym: DIP2378

Alternate gene names: 38234911

Gene position: 2485808-2485137 (Counterclockwise)

Preceding gene: 38234912

Following gene: 38234910

Centisome position: 99.89

GC content: 55.36

Gene sequence:

>672_bases
GTGTGGGGATACACCGTAGAAAAGGGATGGTTTCACGTGAAACATGAAGAAATTGAGTCGATAGCGAGCCACGTGTTCGG
AGGAAACGTCGATAAGGCGTACGCCTACCATGAGCTTTTGGCTACCGACGGCTCCACGCGTGGTTTTATCGGCCCGCGTG
AGGTCCCCAAACTGTGGTCGCGCCACCTTCTTAACTGTGCAGTGATTGGCGAGGCCATGGATGAAAACATCCGCGTCGCC
GACATCGGATCTGGTGCTGGTTTGCCTGGTATCCCCCTTGCCATCGCGCGACCGGACCTGACCATCACCTTGATCGAGCC
GCTTTTGAAGCGATCGGTTTTCCTCGGCGAGGTCAAAGAAAAACTCGAGCTCGACAACGTTACGGTCATTCGTGGACGTG
CGGAGGAAAAAGCGGTGCGCGAGCAGCTCGGGCTTGTCGACGTCGCCACGTCTAGGGCGGTTGCGCCACTAGGAAAACTT
GCCGGCTGGTCACTCCCCCTCGTTCGTCTTGGCGGAAAAATGATCGCCATGAAGGGCTCCTCAGTCCATGAGGAACTTGA
GCGCGACGAAAAAATGATCTGGAAAGCCGGCGGCGGAAAAGTAAAAATCTTTAGTGTCGGTGAAGTTTTGGAAGAACCAA
CCACGCTCATTTCCATCCGCAAGGTGCGATAG

Upstream 100 bases:

>100_bases
GCCGCCTTCCAGATTATCTGGGGGCGGCTTTGCGCTGCTGGGGGTAGGTGTTGGGTGCTCATGCTAGCGTTATTGCGAAG
TATATGAACACGCGTGAGTA

Downstream 100 bases:

>100_bases
CAACTACATGTGGAACCCAGTGCGGTACACGCACTAAGGTAGAAACGTACACACTGTTGGGCTCTTTACTTATATAAAGA
CAGATAAAAAGAACCCTCTT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MWGYTVEKGWFHVKHEEIESIASHVFGGNVDKAYAYHELLATDGSTRGFIGPREVPKLWSRHLLNCAVIGEAMDENIRVA
DIGSGAGLPGIPLAIARPDLTITLIEPLLKRSVFLGEVKEKLELDNVTVIRGRAEEKAVREQLGLVDVATSRAVAPLGKL
AGWSLPLVRLGGKMIAMKGSSVHEELERDEKMIWKAGGGKVKIFSVGEVLEEPTTLISIRKVR

Sequences:

>Translated_223_residues
MWGYTVEKGWFHVKHEEIESIASHVFGGNVDKAYAYHELLATDGSTRGFIGPREVPKLWSRHLLNCAVIGEAMDENIRVA
DIGSGAGLPGIPLAIARPDLTITLIEPLLKRSVFLGEVKEKLELDNVTVIRGRAEEKAVREQLGLVDVATSRAVAPLGKL
AGWSLPLVRLGGKMIAMKGSSVHEELERDEKMIWKAGGGKVKIFSVGEVLEEPTTLISIRKVR
>Mature_223_residues
MWGYTVEKGWFHVKHEEIESIASHVFGGNVDKAYAYHELLATDGSTRGFIGPREVPKLWSRHLLNCAVIGEAMDENIRVA
DIGSGAGLPGIPLAIARPDLTITLIEPLLKRSVFLGEVKEKLELDNVTVIRGRAEEKAVREQLGLVDVATSRAVAPLGKL
AGWSLPLVRLGGKMIAMKGSSVHEELERDEKMIWKAGGGKVKIFSVGEVLEEPTTLISIRKVR

Specific function: Specifically methylates the N7 position of guanosine in position 518 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=156, Percent_Identity=31.4102564102564, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_CORDI (Q6NE98)

Other databases:

- EMBL:   BX248361
- RefSeq:   NP_940678.1
- ProteinModelPortal:   Q6NE98
- GeneID:   2649200
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP2378
- NMPDR:   fig|257309.1.peg.2269
- HOGENOM:   HBG686577
- OMA:   VPRLWER
- PhylomeDB:   Q6NE98
- ProtClustDB:   PRK00107
- BioCyc:   CDIP257309:DIP2378-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 24426; Mature: 24426

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWGYTVEKGWFHVKHEEIESIASHVFGGNVDKAYAYHELLATDGSTRGFIGPREVPKLWS
CCCCEECCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RHLLNCAVIGEAMDENIRVADIGSGAGLPGIPLAIARPDLTITLIEPLLKRSVFLGEVKE
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHH
KLELDNVTVIRGRAEEKAVREQLGLVDVATSRAVAPLGKLAGWSLPLVRLGGKMIAMKGS
HHCCCCEEEEECCHHHHHHHHHHCEEEHHHHHHHHHHHHHCCCCCHHHHHCCEEEEEECC
SVHEELERDEKMIWKAGGGKVKIFSVGEVLEEPTTLISIRKVR
HHHHHHHHHHHEEEECCCCEEEEEEHHHHHHCCHHEEEEEECC
>Mature Secondary Structure
MWGYTVEKGWFHVKHEEIESIASHVFGGNVDKAYAYHELLATDGSTRGFIGPREVPKLWS
CCCCEECCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
RHLLNCAVIGEAMDENIRVADIGSGAGLPGIPLAIARPDLTITLIEPLLKRSVFLGEVKE
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECCCEEEHHHHHHHHHHHHHHHHHH
KLELDNVTVIRGRAEEKAVREQLGLVDVATSRAVAPLGKLAGWSLPLVRLGGKMIAMKGS
HHCCCCEEEEECCHHHHHHHHHHCEEEHHHHHHHHHHHHHCCCCCHHHHHCCEEEEEECC
SVHEELERDEKMIWKAGGGKVKIFSVGEVLEEPTTLISIRKVR
HHHHHHHHHHHEEEECCCCEEEEEEHHHHHHCCHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910