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The map label for this gene is pbpG [H]

Identifier: 38234829

GI number: 38234829

Start: 2390445

End: 2392625

Strand: Reverse

Name: pbpG [H]

Synonym: DIP2294

Alternate gene names: 38234829

Gene position: 2392625-2390445 (Counterclockwise)

Preceding gene: 38234830

Following gene: 38234828

Centisome position: 96.14

GC content: 54.97

Gene sequence:

>2181_bases
TTGGCAGATTCAGGTAAAGGCAAGCCATCTCCCAAGCGGAAGAAGGCCAGTGCCAAAAGTAGCGCGAAAAGTCGTCCGGC
ACGGGCACCGCAGGCGCGTCGCCCGAAAAAGAAGTGGGTTAAGCCAGTTATTGGCGGCAGTATTGCTGCTGTACTGCTGG
CCCCCTTCCTTGCGTTCATGGTCGCATATATTGTGACCGATGTTCCCGAGCCTAGCGAGCTGGTGACCAAACAGGTTTCA
CAGATTTTTGCCTCCGACGGTAAAACGCAGCTGGCGCGTATCGTGCCACCGGACGGAAACCGCACCGAGGTGCCTATCGA
CAAGATCCCCGAGGTTACCCGCAACGCAGTCCTCGCAGCCGAAGACCGTGAGTTCTACACCAACTCCGGTTTCTCCTTTA
CTGGCTTTGGTCGTGCTATCTTGGGCCAGCTCACCGGTGACGCTTCCGCCGGTGGTGGTTCGACAATTACCCAGCAGTAT
GTGAAGAACATGGTGGTGGGCAATGACTACTCCTACATGCGTAAAGCAAAGGAACTGGTGTACTCCGTCAAGATGGCCAA
TGAATGGTCCAAAGACGAAGTTCTCGGTGCATATCTCAATACCATTTACTTCGGCCGCAATGCCTACGGTATCGACGCTG
CCGCCCATGCCTACTTTGGTGTTCCAGCTTCCGAACTCAACGTAGGACAGTCCGCTATGTTGGCCGCCACAATTCAGCGC
CCCAGCCAGCTGGATCCATGGAGCAACCGCGTCGAAGCAGAGCAACGTTGGAACTATGTGCTTGACGGCATGGTGGGCGA
AGGCTGGCTCGATCCCAATGACCGCGCTGGTGTTGTATTCCCCGAGGTCACTGACCCTGCTCAGAACCAGACTTACACCG
AAGCCACAGGTGCAAACGGCCTGATTAAAAACCAGGTCATGAACGAGCTGACCACCCTAGGTATCACTGAGTCCGATGTG
CAGACCCTTGGCCTGCGCATCACCACCACTATCGACGACGCCACCCAGAAGTCCATGGTGGAGGCCGTCGACCGCAACAT
GGCCGACGAGCAATCCAACACCCGTGTCGCCGCCGTCAGTGTGGACCCTAAGACCGGTGCCGTACGTGCCTACTACGGCG
GCGACGATGCCACCGGCTACGACTTTGCCAATGCACCGCTGCAAACCGGTTCGACTTTCAAGATCTTTGGTCTTGCTGCA
GCTGTTCAACAGGGCATTCCAACTTCTACTAACTACAGCTCTGATCCTTACCAACTTCGTGGCGGTATCACTGTTACTAA
CGTTGATAAAGGTTGTGGCGTCTGCTCTCTTAAAGAAGCTTTGAAAAATTCCTACAACACCAGTTTCCTGCGACTGCAAG
ATGATCTGAAACATGGCAGCCAAGACACAGCTGACATGGCGCACTCCCTTGGTGTTGCTCGTTCCTTGCCCGGAATTGAA
AAAACCCTGACCGAAAATGGTCAGCAGCCCTATGAAGGCGTCATCCTTGGCCAGTACCTTTCTCGTCCGCTGGATATGGC
TCATGCTATGGCCACTTTGGCTAATTATGGTGTTTGGCACCAAGAGTACTTTGTAGAAAAGATTGAAACTGCCGAAGGTA
AACTGCTCTACCAGCATGAAGGTACCCAAGGTGAACGGCGTGTTTCCGAGAAGGTAGCCACTAACGTTCTTGATGCGATG
GCTCCTATTGCAGCATGGTCCGGTGGAAACGGTCTTGCAGGTGGGCGTCCATCGGCAAGCAAAACCGGAACGACTCAGCT
TGGCGATACCGGCGCTAATAAAGATGCTTGGATGATCGGTGCCACCCCGCAACTTGCAACCGCGGTCTGGGCGGGTACCG
CCGATAACTCGCCATTGCTGAATTCGTGGGGTGGGCCTATGTACGGCTCCAACCTCCCTGCACAGTTATGGAAGGATATC
AGTGACTCTGCTCTTTCAGGTAAAGATTGGGAGCAATTCAAACCCGCACAAAATATTGGCTGGGCGAAATATGGCTACGG
AACTGCTCCTCGTGCGACCACTGAAAAGATTCAAACTGAGACCTCGACCAGCTCGGCGGAGGCTACCAGCCCTGCAGCAT
CTGGAACACCGGGTGCACCGCCAGTACCAAGTGCCCCAGAGCCGGCGCCAGCTCCGGGAGGCATTGAGATTCTGCCAGGC
GTAGTCCTGCCGGTCCCATAA

Upstream 100 bases:

>100_bases
TATGCCACAGTAACGGTTGTGTGCATCTCCCGTTGGACTATTTTGGTATGTAAAGTGTGGGTGTTGAGCTCAACCGCAGA
TCAAGGAAGCATGAACAAAG

Downstream 100 bases:

>100_bases
CCTAGTCCTCCCGAAGTGCCTCCTGATTCTTACAGGGGGCACTTTTGTATGCCATGCGCCAGCCCGTGACGAATTTTTGT
ACCCTAAGAAAATGTGCAGC

Product: putative penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 726; Mature: 725

Protein sequence:

>726_residues
MADSGKGKPSPKRKKASAKSSAKSRPARAPQARRPKKKWVKPVIGGSIAAVLLAPFLAFMVAYIVTDVPEPSELVTKQVS
QIFASDGKTQLARIVPPDGNRTEVPIDKIPEVTRNAVLAAEDREFYTNSGFSFTGFGRAILGQLTGDASAGGGSTITQQY
VKNMVVGNDYSYMRKAKELVYSVKMANEWSKDEVLGAYLNTIYFGRNAYGIDAAAHAYFGVPASELNVGQSAMLAATIQR
PSQLDPWSNRVEAEQRWNYVLDGMVGEGWLDPNDRAGVVFPEVTDPAQNQTYTEATGANGLIKNQVMNELTTLGITESDV
QTLGLRITTTIDDATQKSMVEAVDRNMADEQSNTRVAAVSVDPKTGAVRAYYGGDDATGYDFANAPLQTGSTFKIFGLAA
AVQQGIPTSTNYSSDPYQLRGGITVTNVDKGCGVCSLKEALKNSYNTSFLRLQDDLKHGSQDTADMAHSLGVARSLPGIE
KTLTENGQQPYEGVILGQYLSRPLDMAHAMATLANYGVWHQEYFVEKIETAEGKLLYQHEGTQGERRVSEKVATNVLDAM
APIAAWSGGNGLAGGRPSASKTGTTQLGDTGANKDAWMIGATPQLATAVWAGTADNSPLLNSWGGPMYGSNLPAQLWKDI
SDSALSGKDWEQFKPAQNIGWAKYGYGTAPRATTEKIQTETSTSSAEATSPAASGTPGAPPVPSAPEPAPAPGGIEILPG
VVLPVP

Sequences:

>Translated_726_residues
MADSGKGKPSPKRKKASAKSSAKSRPARAPQARRPKKKWVKPVIGGSIAAVLLAPFLAFMVAYIVTDVPEPSELVTKQVS
QIFASDGKTQLARIVPPDGNRTEVPIDKIPEVTRNAVLAAEDREFYTNSGFSFTGFGRAILGQLTGDASAGGGSTITQQY
VKNMVVGNDYSYMRKAKELVYSVKMANEWSKDEVLGAYLNTIYFGRNAYGIDAAAHAYFGVPASELNVGQSAMLAATIQR
PSQLDPWSNRVEAEQRWNYVLDGMVGEGWLDPNDRAGVVFPEVTDPAQNQTYTEATGANGLIKNQVMNELTTLGITESDV
QTLGLRITTTIDDATQKSMVEAVDRNMADEQSNTRVAAVSVDPKTGAVRAYYGGDDATGYDFANAPLQTGSTFKIFGLAA
AVQQGIPTSTNYSSDPYQLRGGITVTNVDKGCGVCSLKEALKNSYNTSFLRLQDDLKHGSQDTADMAHSLGVARSLPGIE
KTLTENGQQPYEGVILGQYLSRPLDMAHAMATLANYGVWHQEYFVEKIETAEGKLLYQHEGTQGERRVSEKVATNVLDAM
APIAAWSGGNGLAGGRPSASKTGTTQLGDTGANKDAWMIGATPQLATAVWAGTADNSPLLNSWGGPMYGSNLPAQLWKDI
SDSALSGKDWEQFKPAQNIGWAKYGYGTAPRATTEKIQTETSTSSAEATSPAASGTPGAPPVPSAPEPAPAPGGIEILPG
VVLPVP
>Mature_725_residues
ADSGKGKPSPKRKKASAKSSAKSRPARAPQARRPKKKWVKPVIGGSIAAVLLAPFLAFMVAYIVTDVPEPSELVTKQVSQ
IFASDGKTQLARIVPPDGNRTEVPIDKIPEVTRNAVLAAEDREFYTNSGFSFTGFGRAILGQLTGDASAGGGSTITQQYV
KNMVVGNDYSYMRKAKELVYSVKMANEWSKDEVLGAYLNTIYFGRNAYGIDAAAHAYFGVPASELNVGQSAMLAATIQRP
SQLDPWSNRVEAEQRWNYVLDGMVGEGWLDPNDRAGVVFPEVTDPAQNQTYTEATGANGLIKNQVMNELTTLGITESDVQ
TLGLRITTTIDDATQKSMVEAVDRNMADEQSNTRVAAVSVDPKTGAVRAYYGGDDATGYDFANAPLQTGSTFKIFGLAAA
VQQGIPTSTNYSSDPYQLRGGITVTNVDKGCGVCSLKEALKNSYNTSFLRLQDDLKHGSQDTADMAHSLGVARSLPGIEK
TLTENGQQPYEGVILGQYLSRPLDMAHAMATLANYGVWHQEYFVEKIETAEGKLLYQHEGTQGERRVSEKVATNVLDAMA
PIAAWSGGNGLAGGRPSASKTGTTQLGDTGANKDAWMIGATPQLATAVWAGTADNSPLLNSWGGPMYGSNLPAQLWKDIS
DSALSGKDWEQFKPAQNIGWAKYGYGTAPRATTEKIQTETSTSSAEATSPAASGTPGAPPVPSAPEPAPAPGGIEILPGV
VLPVP

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=572, Percent_Identity=29.5454545454545, Blast_Score=161, Evalue=2e-40,
Organism=Escherichia coli, GI1788867, Length=583, Percent_Identity=27.4442538593482, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI87082258, Length=332, Percent_Identity=29.5180722891566, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1789601, Length=211, Percent_Identity=31.2796208530806, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 77241; Mature: 77110

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSGKGKPSPKRKKASAKSSAKSRPARAPQARRPKKKWVKPVIGGSIAAVLLAPFLAFM
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
VAYIVTDVPEPSELVTKQVSQIFASDGKTQLARIVPPDGNRTEVPIDKIPEVTRNAVLAA
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHCCHHHHCCEEEE
EDREFYTNSGFSFTGFGRAILGQLTGDASAGGGSTITQQYVKNMVVGNDYSYMRKAKELV
CCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
YSVKMANEWSKDEVLGAYLNTIYFGRNAYGIDAAAHAYFGVPASELNVGQSAMLAATIQR
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHCCCHHHHHHHHHCC
PSQLDPWSNRVEAEQRWNYVLDGMVGEGWLDPNDRAGVVFPEVTDPAQNQTYTEATGANG
CCCCCCHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCC
LIKNQVMNELTTLGITESDVQTLGLRITTTIDDATQKSMVEAVDRNMADEQSNTRVAAVS
HHHHHHHHHHHHCCCCHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEE
VDPKTGAVRAYYGGDDATGYDFANAPLQTGSTFKIFGLAAAVQQGIPTSTNYSSDPYQLR
ECCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEEE
GGITVTNVDKGCGVCSLKEALKNSYNTSFLRLQDDLKHGSQDTADMAHSLGVARSLPGIE
CCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHCCCCHH
KTLTENGQQPYEGVILGQYLSRPLDMAHAMATLANYGVWHQEYFVEKIETAEGKLLYQHE
HHHHHCCCCCCCCEEEHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEC
GTQGERRVSEKVATNVLDAMAPIAAWSGGNGLAGGRPSASKTGTTQLGDTGANKDAWMIG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
ATPQLATAVWAGTADNSPLLNSWGGPMYGSNLPAQLWKDISDSALSGKDWEQFKPAQNIG
CCHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCHHCCC
WAKYGYGTAPRATTEKIQTETSTSSAEATSPAASGTPGAPPVPSAPEPAPAPGGIEILPG
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCC
VVLPVP
EEECCC
>Mature Secondary Structure 
ADSGKGKPSPKRKKASAKSSAKSRPARAPQARRPKKKWVKPVIGGSIAAVLLAPFLAFM
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
VAYIVTDVPEPSELVTKQVSQIFASDGKTQLARIVPPDGNRTEVPIDKIPEVTRNAVLAA
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHCCHHHHCCEEEE
EDREFYTNSGFSFTGFGRAILGQLTGDASAGGGSTITQQYVKNMVVGNDYSYMRKAKELV
CCCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHH
YSVKMANEWSKDEVLGAYLNTIYFGRNAYGIDAAAHAYFGVPASELNVGQSAMLAATIQR
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHHHHCCCHHHHHHHHHCC
PSQLDPWSNRVEAEQRWNYVLDGMVGEGWLDPNDRAGVVFPEVTDPAQNQTYTEATGANG
CCCCCCHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCC
LIKNQVMNELTTLGITESDVQTLGLRITTTIDDATQKSMVEAVDRNMADEQSNTRVAAVS
HHHHHHHHHHHHCCCCHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEE
VDPKTGAVRAYYGGDDATGYDFANAPLQTGSTFKIFGLAAAVQQGIPTSTNYSSDPYQLR
ECCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEEE
GGITVTNVDKGCGVCSLKEALKNSYNTSFLRLQDDLKHGSQDTADMAHSLGVARSLPGIE
CCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHCCCCHH
KTLTENGQQPYEGVILGQYLSRPLDMAHAMATLANYGVWHQEYFVEKIETAEGKLLYQHE
HHHHHCCCCCCCCEEEHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEC
GTQGERRVSEKVATNVLDAMAPIAAWSGGNGLAGGRPSASKTGTTQLGDTGANKDAWMIG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
ATPQLATAVWAGTADNSPLLNSWGGPMYGSNLPAQLWKDISDSALSGKDWEQFKPAQNIG
CCHHHHHHHCCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCHHCCC
WAKYGYGTAPRATTEKIQTETSTSSAEATSPAASGTPGAPPVPSAPEPAPAPGGIEILPG
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCC
VVLPVP
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]