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The map label for this gene is fadD26 [H]

Identifier: 38234731

GI number: 38234731

Start: 2272766

End: 2274580

Strand: Reverse

Name: fadD26 [H]

Synonym: DIP2190

Alternate gene names: 38234731

Gene position: 2274580-2272766 (Counterclockwise)

Preceding gene: 38234732

Following gene: 38234730

Centisome position: 91.4

GC content: 56.58

Gene sequence:

>1815_bases
ATGGATCTGCATAAAGCCATGGGCCAGTTCTTTGACGCCAAGGGTAATATTGCGCTACCGCCACAAATTACGCTCGCAGG
TCTGAGCGAATTGTTCTACCAGTCGGATGTTGATGGCGGCGATCGTCATTGCATGCGCTACTGGGATTACAGTACTGAAG
GTGGTGTGGCTCGTGACTACAACCGCCGCGAGATCAACACTCGCATTAAGTCGGTGGCAGCACGCCTCCAGCAGGTGGCA
CAGCCTGGCGACCGTGTAGCTATTTTGGCTAACAACAGCCCTGAGTACCTCTTTGGCTTTATCGGCGCTTTGTATGCGGG
TCTGGTGCCTGTTCCGCTGTATGATCCTTCGGAGCCAGGGCATGCCGATCACCTCACCGCTGTGATGGCTGACGCGCAGC
CAGCTATCGTGCTGACGAACAATGCGTCGGCTGCGGCGGTTCGTCGTTTCTTCTCTGCGCTGCCAGGTGCACAGCGCCCT
CGTATTATCTCCATTGATTCGCTGCCGGATACGCTTGCGCAAAGCTATCAGATCCCTACGCCGTCGATGGCTGCTGCTGC
GATCAACCCTGTTGATCTGCCAGCATTCTTGCAGTACACCTCAGGTTCTACCCGCACCCCTGCCGGTGTGGTACTGACCA
ACCGTTCGATCGTGACCAACGTGCTGCAGATTTTCGCCGCGGCTCAGTTGAAGACTCCGCTGCGTTTGGTGTCGTGGCTG
CCGCTGCACCATGACATGGGCATCATTTTGGCTGTGTTTGTTACGTTGTTGGGCTTGGAGTTCGAGATGATGTCGCCACG
CGACTTTATCCAGCAGCCAAAGCGTTGGATTGACCAGCTGGATCGTCGTGAGGGCGATAACGCGATCTACGCTGTGGTTC
CGAACTTCGCACTCGAGCTTGCTGCACGTTATGCAACGCCTTCTGAGGGCTTGGATCTTTCTGCTGTTGAGGGCCTAATT
ATTGGTTCGGAGCCGGTCACAGAGAAGGCTGTGGAGGCATTCGCTGAGAGGTTCGGGCCTTATGGTTTGAACAGGCAGAA
CATGCGCCCGTCGTATGGTCTTGCTGAGGCATCACTGCTGGTAACTACCCCGCAGACCCCGCAGCGCCCTGTGATTTCTT
ACTTTGATCGTGAGCAGTTGGCTGCAAACCGTGCGGTGATCGTCGACAAGGGCGATAACGCTGTGGCGTTGACCGGTGTG
GGGCAGGTTGTGCGCCCACAGAACTTGGTCATCGTGGATCCTGAGACGCGCACCGAGGTTGCAGATGGCACTATCGGTGA
GCTGTGGGCGCATGGTGAGAACATGGCTGCTGGCTACTTGGATCGTCCAGAAGATACCGCAGAGACGTTCCACAACACCT
TGGCTGGTCGTTTGGAGAACTCCCGTGCGGCAGGTGTGCCGGAGGAGTCCACGAAGTGGATGGCGACCGGTGACCTTGGT
GTGATTGTGGACGGTGAACTCTACATCACGGGTCGTTTGAAGGATCTTGTGATTATTGCGGGTCGTAACCACTATCCGCA
GGATATTGAGTACACGGTGGATCATGCTTCGGAGCACATCCGCCCTGCTGCAGTGGCTGCCTTTGCTATTGAGGGCGACG
CCGTGGAGCAGCTGATCATCTTGGCGGAGCGCGATCTGGATCGTGATCCATCTGGTGATGCGGAGGCTATCGACGCTATC
CGTGCTGCCGTCACGGAGGCTCATGGTGTGGTTCCTGCGGATATCCGTATCGTGGCCCCAGATGAGATCTTGCGTTCTTC
CTCCGGCAAGATTGCTCGCCGCGTGAATAAGAAGGCGTATCAAGAATCCCACTAG

Upstream 100 bases:

>100_bases
GGATCAAGGGACTTATCGACGGATAACCCAACGTCCATGTAACATTTTTGTCATCGAAAACAATATGTTGCATGGACGTT
GTTTACATTGAAGGAGACGT

Downstream 100 bases:

>100_bases
ATTTTTTGGGATATTGCCCCGCGCGTGAAACATTTGTGACGCGTGAGACGATATAGAATGGAACGACTGTCTCGTCTGCA
ATATTCTGAGGTTAAAGTAT

Product: acyl-CoA synthase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MDLHKAMGQFFDAKGNIALPPQITLAGLSELFYQSDVDGGDRHCMRYWDYSTEGGVARDYNRREINTRIKSVAARLQQVA
QPGDRVAILANNSPEYLFGFIGALYAGLVPVPLYDPSEPGHADHLTAVMADAQPAIVLTNNASAAAVRRFFSALPGAQRP
RIISIDSLPDTLAQSYQIPTPSMAAAAINPVDLPAFLQYTSGSTRTPAGVVLTNRSIVTNVLQIFAAAQLKTPLRLVSWL
PLHHDMGIILAVFVTLLGLEFEMMSPRDFIQQPKRWIDQLDRREGDNAIYAVVPNFALELAARYATPSEGLDLSAVEGLI
IGSEPVTEKAVEAFAERFGPYGLNRQNMRPSYGLAEASLLVTTPQTPQRPVISYFDREQLAANRAVIVDKGDNAVALTGV
GQVVRPQNLVIVDPETRTEVADGTIGELWAHGENMAAGYLDRPEDTAETFHNTLAGRLENSRAAGVPEESTKWMATGDLG
VIVDGELYITGRLKDLVIIAGRNHYPQDIEYTVDHASEHIRPAAVAAFAIEGDAVEQLIILAERDLDRDPSGDAEAIDAI
RAAVTEAHGVVPADIRIVAPDEILRSSSGKIARRVNKKAYQESH

Sequences:

>Translated_604_residues
MDLHKAMGQFFDAKGNIALPPQITLAGLSELFYQSDVDGGDRHCMRYWDYSTEGGVARDYNRREINTRIKSVAARLQQVA
QPGDRVAILANNSPEYLFGFIGALYAGLVPVPLYDPSEPGHADHLTAVMADAQPAIVLTNNASAAAVRRFFSALPGAQRP
RIISIDSLPDTLAQSYQIPTPSMAAAAINPVDLPAFLQYTSGSTRTPAGVVLTNRSIVTNVLQIFAAAQLKTPLRLVSWL
PLHHDMGIILAVFVTLLGLEFEMMSPRDFIQQPKRWIDQLDRREGDNAIYAVVPNFALELAARYATPSEGLDLSAVEGLI
IGSEPVTEKAVEAFAERFGPYGLNRQNMRPSYGLAEASLLVTTPQTPQRPVISYFDREQLAANRAVIVDKGDNAVALTGV
GQVVRPQNLVIVDPETRTEVADGTIGELWAHGENMAAGYLDRPEDTAETFHNTLAGRLENSRAAGVPEESTKWMATGDLG
VIVDGELYITGRLKDLVIIAGRNHYPQDIEYTVDHASEHIRPAAVAAFAIEGDAVEQLIILAERDLDRDPSGDAEAIDAI
RAAVTEAHGVVPADIRIVAPDEILRSSSGKIARRVNKKAYQESH
>Mature_604_residues
MDLHKAMGQFFDAKGNIALPPQITLAGLSELFYQSDVDGGDRHCMRYWDYSTEGGVARDYNRREINTRIKSVAARLQQVA
QPGDRVAILANNSPEYLFGFIGALYAGLVPVPLYDPSEPGHADHLTAVMADAQPAIVLTNNASAAAVRRFFSALPGAQRP
RIISIDSLPDTLAQSYQIPTPSMAAAAINPVDLPAFLQYTSGSTRTPAGVVLTNRSIVTNVLQIFAAAQLKTPLRLVSWL
PLHHDMGIILAVFVTLLGLEFEMMSPRDFIQQPKRWIDQLDRREGDNAIYAVVPNFALELAARYATPSEGLDLSAVEGLI
IGSEPVTEKAVEAFAERFGPYGLNRQNMRPSYGLAEASLLVTTPQTPQRPVISYFDREQLAANRAVIVDKGDNAVALTGV
GQVVRPQNLVIVDPETRTEVADGTIGELWAHGENMAAGYLDRPEDTAETFHNTLAGRLENSRAAGVPEESTKWMATGDLG
VIVDGELYITGRLKDLVIIAGRNHYPQDIEYTVDHASEHIRPAAVAAFAIEGDAVEQLIILAERDLDRDPSGDAEAIDAI
RAAVTEAHGVVPADIRIVAPDEILRSSSGKIARRVNKKAYQESH

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=538, Percent_Identity=22.6765799256506, Blast_Score=110, Evalue=6e-24,
Organism=Homo sapiens, GI55749758, Length=477, Percent_Identity=23.4800838574423, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI225735629, Length=517, Percent_Identity=22.2437137330754, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI45827692, Length=517, Percent_Identity=22.2437137330754, Blast_Score=91, Evalue=4e-18,
Organism=Escherichia coli, GI1788107, Length=602, Percent_Identity=26.578073089701, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI193202679, Length=544, Percent_Identity=21.6911764705882, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17559526, Length=553, Percent_Identity=22.9656419529837, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI18859661, Length=572, Percent_Identity=25.5244755244755, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24654656, Length=582, Percent_Identity=20.9621993127148, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 65668; Mature: 65668

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLHKAMGQFFDAKGNIALPPQITLAGLSELFYQSDVDGGDRHCMRYWDYSTEGGVARDY
CCHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCC
NRREINTRIKSVAARLQQVAQPGDRVAILANNSPEYLFGFIGALYAGLVPVPLYDPSEPG
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
HADHLTAVMADAQPAIVLTNNASAAAVRRFFSALPGAQRPRIISIDSLPDTLAQSYQIPT
CCHHHHHHEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCC
PSMAAAAINPVDLPAFLQYTSGSTRTPAGVVLTNRSIVTNVLQIFAAAQLKTPLRLVSWL
CCHHHHHCCCCCCHHHEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHC
PLHHDMGIILAVFVTLLGLEFEMMSPRDFIQQPKRWIDQLDRREGDNAIYAVVPNFALEL
CCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHH
AARYATPSEGLDLSAVEGLIIGSEPVTEKAVEAFAERFGPYGLNRQNMRPSYGLAEASLL
HHHHCCCCCCCCHHHHCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
VTTPQTPQRPVISYFDREQLAANRAVIVDKGDNAVALTGVGQVVRPQNLVIVDPETRTEV
EECCCCCCCHHHHHHCHHHHCCCCEEEEECCCCEEEEECCCCEECCCCEEEECCCCCCHH
ADGTIGELWAHGENMAAGYLDRPEDTAETFHNTLAGRLENSRAAGVPEESTKWMATGDLG
CCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEE
VIVDGELYITGRLKDLVIIAGRNHYPQDIEYTVDHASEHIRPAAVAAFAIEGDAVEQLII
EEECCEEEEEECCEEEEEEECCCCCCCCCCEEHHHHHHCCCHHHHEEEEECCHHHHHHHH
LAERDLDRDPSGDAEAIDAIRAAVTEAHGVVPADIRIVAPDEILRSSSGKIARRVNKKAY
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCCHHHHHHHHHHH
QESH
CCCC
>Mature Secondary Structure
MDLHKAMGQFFDAKGNIALPPQITLAGLSELFYQSDVDGGDRHCMRYWDYSTEGGVARDY
CCHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCC
NRREINTRIKSVAARLQQVAQPGDRVAILANNSPEYLFGFIGALYAGLVPVPLYDPSEPG
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
HADHLTAVMADAQPAIVLTNNASAAAVRRFFSALPGAQRPRIISIDSLPDTLAQSYQIPT
CCHHHHHHEECCCCEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCC
PSMAAAAINPVDLPAFLQYTSGSTRTPAGVVLTNRSIVTNVLQIFAAAQLKTPLRLVSWL
CCHHHHHCCCCCCHHHEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHCCHHHHHHHC
PLHHDMGIILAVFVTLLGLEFEMMSPRDFIQQPKRWIDQLDRREGDNAIYAVVPNFALEL
CCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHH
AARYATPSEGLDLSAVEGLIIGSEPVTEKAVEAFAERFGPYGLNRQNMRPSYGLAEASLL
HHHHCCCCCCCCHHHHCEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
VTTPQTPQRPVISYFDREQLAANRAVIVDKGDNAVALTGVGQVVRPQNLVIVDPETRTEV
EECCCCCCCHHHHHHCHHHHCCCCEEEEECCCCEEEEECCCCEECCCCEEEECCCCCCHH
ADGTIGELWAHGENMAAGYLDRPEDTAETFHNTLAGRLENSRAAGVPEESTKWMATGDLG
CCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEE
VIVDGELYITGRLKDLVIIAGRNHYPQDIEYTVDHASEHIRPAAVAAFAIEGDAVEQLII
EEECCEEEEEECCEEEEEEECCCCCCCCCCEEHHHHHHCCCHHHHEEEEECCHHHHHHHH
LAERDLDRDPSGDAEAIDAIRAAVTEAHGVVPADIRIVAPDEILRSSSGKIARRVNKKAY
HHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHCCCCHHHHHHHHHHH
QESH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]