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The map label for this gene is folC [H]

Identifier: 38234356

GI number: 38234356

Start: 1828855

End: 1830351

Strand: Reverse

Name: folC [H]

Synonym: DIP1785

Alternate gene names: 38234356

Gene position: 1830351-1828855 (Counterclockwise)

Preceding gene: 38234357

Following gene: 38234355

Centisome position: 73.55

GC content: 59.52

Gene sequence:

>1497_bases
GTGAAAGACGAGGAGCTCCACCTAGGAGAAGTCTCCCTGAACGAGACTGGCCTATCGCTGCCTATCGACGTCAACGCGGA
CACCGAAGCACCCGCCCCGCGCGAGATCACGGCCGATGACCTCATGGATTTGGCCACCGTCGAAGCGGAGCTGGATCAGC
GCTGGTCCGAGGTCAAAATTGACCCCACGCTCGAGCGTGTGGAGATGTTGATGGATCTGCTGGGTCACCCAGAACATGCG
TTTCCGGCTATTCACGTAGCCGGGACCAACGGCAAAACGTCGACGGTACGCATGATTGAATCCCTCATGCGTGCTTTCCA
CCGCCGCACGGGACGCACCACCAGCCCGCACCTTCAGCTGGTCACCGAGCGCATTGCTATCGACGGCCAGCCGATCCACC
CTCGCGACTATGTACGCACGTGGAACGAGATCAAACCCTATGTGGAGATGGTCGACGCTCATTCTGAGGCCAACGGTGGC
CCCAAGATGAGCAAGTTCGAAGTGCTCACGGCCATGGCCTACGCTGCGTTTGCAGACGCACCAGTGGACGTTGCAGTGGT
GGAAGTGGGCATGGGTGGTCGCTGGGATGCCACCAATGTGATCAATTCTGATGTTGCTGTGGTTATGCCCGTGGGGCTGG
ACCACACTGATTTTCTTGGAGATACCATCGAGAAAATCGCTGCTGAAAAAGCTGGCATCATCAAGTCCCGTTGGGATGCC
TCCGACCTGCTGACCCCGCCGGATAACGTCGCTATCGTTGCGGAGCAAGATCCCGCGGCGATGAACGTGATCCTTGAACA
GGCCGTGAGCGTGGATGCTTCTGTGGCGCGCGCCGGTGTGGAATTTGGGGTGATGGATGCTGCTATCGCGGTTGGTGGTC
AACAGATCAGTATTCGTGGCCTAGGTGGCGAGTACCCCGACATCTTCTTGCCACTTTCTGGCGAGCACCAAGCCCGTAAC
GCAGCGACCGCCTTGGCTGCTGTGGAGGCCTTCTTTGGTGCGGGTGCTGGCCGCCAGCTAGACCTTGATACGGTTCGCGA
AGGTTTTGCTACCGTTCAGTCCCCAGGTCGTCTCGAGCGGGTGCGCACTGCTCCCACGGTATTTATCGACGCTGCCCACA
ACCCTCACGGTGCTGCGGCACTGGGTAAGGCCCTAGAGCGCGACTTCGATTTCTCGCGCCTCATTGGCGTGGTAGCAGTG
CTTGGCGACAAAGATTACGTGGGCGTGTTGCGTGCCCTGGAACCTCATCTATCCGAGATCGTGGTGACCACGAACTCTTC
GCCGCGCGCTCTTGCTGCCGATGACCTTGCCGAGGAAGCACGCAACGTCTTTGGCGAAGAACGCGTGCACGTGGCGTACT
CGCTGCCGTCGGCAGTCGAACTGGCAGTGGAACTTGCCGAGGATACCGAGGTGCAATCCGGTTCCGGTGTAATCGTCACA
GGCTCTGTGGTCACTGCGGGTGAGGCCCGTACCCTGTTTGGAAAGGACCCCGTATGA

Upstream 100 bases:

>100_bases
TGGCCAAGGCACCAGAGGCCGTGGTGGCAAAGATTCGCGAGCGTCAGCAGATCGCCCAAGAAGAGGTGGCCCGCATTAGC
GCTCGCCTTGAGGAGCTGAA

Downstream 100 bases:

>100_bases
GTGATGAGCAATATGGACCGTTAGGTCCAGGACATGCGCCGGTGAAAGATCCGATGAAAGGTCTTCGCGGAGTCATGGCT
GGAACGATGATGATGGAGGC

Product: Mur ligase family protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MKDEELHLGEVSLNETGLSLPIDVNADTEAPAPREITADDLMDLATVEAELDQRWSEVKIDPTLERVEMLMDLLGHPEHA
FPAIHVAGTNGKTSTVRMIESLMRAFHRRTGRTTSPHLQLVTERIAIDGQPIHPRDYVRTWNEIKPYVEMVDAHSEANGG
PKMSKFEVLTAMAYAAFADAPVDVAVVEVGMGGRWDATNVINSDVAVVMPVGLDHTDFLGDTIEKIAAEKAGIIKSRWDA
SDLLTPPDNVAIVAEQDPAAMNVILEQAVSVDASVARAGVEFGVMDAAIAVGGQQISIRGLGGEYPDIFLPLSGEHQARN
AATALAAVEAFFGAGAGRQLDLDTVREGFATVQSPGRLERVRTAPTVFIDAAHNPHGAAALGKALERDFDFSRLIGVVAV
LGDKDYVGVLRALEPHLSEIVVTTNSSPRALAADDLAEEARNVFGEERVHVAYSLPSAVELAVELAEDTEVQSGSGVIVT
GSVVTAGEARTLFGKDPV

Sequences:

>Translated_498_residues
MKDEELHLGEVSLNETGLSLPIDVNADTEAPAPREITADDLMDLATVEAELDQRWSEVKIDPTLERVEMLMDLLGHPEHA
FPAIHVAGTNGKTSTVRMIESLMRAFHRRTGRTTSPHLQLVTERIAIDGQPIHPRDYVRTWNEIKPYVEMVDAHSEANGG
PKMSKFEVLTAMAYAAFADAPVDVAVVEVGMGGRWDATNVINSDVAVVMPVGLDHTDFLGDTIEKIAAEKAGIIKSRWDA
SDLLTPPDNVAIVAEQDPAAMNVILEQAVSVDASVARAGVEFGVMDAAIAVGGQQISIRGLGGEYPDIFLPLSGEHQARN
AATALAAVEAFFGAGAGRQLDLDTVREGFATVQSPGRLERVRTAPTVFIDAAHNPHGAAALGKALERDFDFSRLIGVVAV
LGDKDYVGVLRALEPHLSEIVVTTNSSPRALAADDLAEEARNVFGEERVHVAYSLPSAVELAVELAEDTEVQSGSGVIVT
GSVVTAGEARTLFGKDPV
>Mature_498_residues
MKDEELHLGEVSLNETGLSLPIDVNADTEAPAPREITADDLMDLATVEAELDQRWSEVKIDPTLERVEMLMDLLGHPEHA
FPAIHVAGTNGKTSTVRMIESLMRAFHRRTGRTTSPHLQLVTERIAIDGQPIHPRDYVRTWNEIKPYVEMVDAHSEANGG
PKMSKFEVLTAMAYAAFADAPVDVAVVEVGMGGRWDATNVINSDVAVVMPVGLDHTDFLGDTIEKIAAEKAGIIKSRWDA
SDLLTPPDNVAIVAEQDPAAMNVILEQAVSVDASVARAGVEFGVMDAAIAVGGQQISIRGLGGEYPDIFLPLSGEHQARN
AATALAAVEAFFGAGAGRQLDLDTVREGFATVQSPGRLERVRTAPTVFIDAAHNPHGAAALGKALERDFDFSRLIGVVAV
LGDKDYVGVLRALEPHLSEIVVTTNSSPRALAADDLAEEARNVFGEERVHVAYSLPSAVELAVELAEDTEVQSGSGVIVT
GSVVTAGEARTLFGKDPV

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=363, Percent_Identity=32.5068870523416, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI66932990, Length=363, Percent_Identity=32.5068870523416, Blast_Score=149, Evalue=7e-36,
Organism=Escherichia coli, GI1788654, Length=419, Percent_Identity=33.890214797136, Blast_Score=167, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71984923, Length=320, Percent_Identity=29.0625, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17553148, Length=320, Percent_Identity=29.0625, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17553150, Length=320, Percent_Identity=29.0625, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323760, Length=377, Percent_Identity=30.7692307692308, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324815, Length=219, Percent_Identity=32.4200913242009, Blast_Score=106, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24641571, Length=237, Percent_Identity=32.9113924050633, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24581568, Length=200, Percent_Identity=31.5, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 53164; Mature: 53164

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDEELHLGEVSLNETGLSLPIDVNADTEAPAPREITADDLMDLATVEAELDQRWSEVKI
CCCCCEEECEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHEEC
DPTLERVEMLMDLLGHPEHAFPAIHVAGTNGKTSTVRMIESLMRAFHRRTGRTTSPHLQL
CCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VTERIAIDGQPIHPRDYVRTWNEIKPYVEMVDAHSEANGGPKMSKFEVLTAMAYAAFADA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PVDVAVVEVGMGGRWDATNVINSDVAVVMPVGLDHTDFLGDTIEKIAAEKAGIIKSRWDA
CCEEEEEEECCCCCCCCCHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHCCCCC
SDLLTPPDNVAIVAEQDPAAMNVILEQAVSVDASVARAGVEFGVMDAAIAVGGQQISIRG
CCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCEEEEEE
LGGEYPDIFLPLSGEHQARNAATALAAVEAFFGAGAGRQLDLDTVREGFATVQSPGRLER
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHCCCCHHHH
VRTAPTVFIDAAHNPHGAAALGKALERDFDFSRLIGVVAVLGDKDYVGVLRALEPHLSEI
HHCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEE
VVTTNSSPRALAADDLAEEARNVFGEERVHVAYSLPSAVELAVELAEDTEVQSGSGVIVT
EEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEE
GSVVTAGEARTLFGKDPV
CCEEECCCCEECCCCCCC
>Mature Secondary Structure
MKDEELHLGEVSLNETGLSLPIDVNADTEAPAPREITADDLMDLATVEAELDQRWSEVKI
CCCCCEEECEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHEEC
DPTLERVEMLMDLLGHPEHAFPAIHVAGTNGKTSTVRMIESLMRAFHRRTGRTTSPHLQL
CCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHH
VTERIAIDGQPIHPRDYVRTWNEIKPYVEMVDAHSEANGGPKMSKFEVLTAMAYAAFADA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PVDVAVVEVGMGGRWDATNVINSDVAVVMPVGLDHTDFLGDTIEKIAAEKAGIIKSRWDA
CCEEEEEEECCCCCCCCCHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHCCCCC
SDLLTPPDNVAIVAEQDPAAMNVILEQAVSVDASVARAGVEFGVMDAAIAVGGQQISIRG
CCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCEEEEEE
LGGEYPDIFLPLSGEHQARNAATALAAVEAFFGAGAGRQLDLDTVREGFATVQSPGRLER
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHHCCCCHHHH
VRTAPTVFIDAAHNPHGAAALGKALERDFDFSRLIGVVAVLGDKDYVGVLRALEPHLSEI
HHCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCEEE
VVTTNSSPRALAADDLAEEARNVFGEERVHVAYSLPSAVELAVELAEDTEVQSGSGVIVT
EEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEE
GSVVTAGEARTLFGKDPV
CCEEECCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]