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The map label for this gene is proB

Identifier: 38234348

GI number: 38234348

Start: 1820793

End: 1821923

Strand: Reverse

Name: proB

Synonym: DIP1777

Alternate gene names: 38234348

Gene position: 1821923-1820793 (Counterclockwise)

Preceding gene: 38234349

Following gene: 38234347

Centisome position: 73.21

GC content: 58.0

Gene sequence:

>1131_bases
ATGAATGCCCAGCACAGCAGCGCCGATCTGCGCGACGTGATTAGAAACGCTAAGCGGATTGTGGTCAAGATTGGTTCTAG
TTCACTGACCGGCGAAAATTTTGAGGTCTCTCCGGGTCGTATCGACCGCATTGTGGAGGCTCTAGAAGATCGTATGCATC
GCGGTTCCGACGTGATTGTTGTATCTTCTGGTGCTGTGGCTGCTGGTATGGCTCCATTGGGGTTGACTTCGCGGCCGAAG
GATTTGGCAACAAAGCAAGCTGCTGCCTCGGTGGGGCAGGTGCATTTGGCTAATGCGTGGGGTAATAGTTTTGCCCGTTA
TAACCGCACGATTGGCCAGGTGCTGTTAACGGCCTCCGACGCAGGCCAGCGGGATCGTGCGCGTAATGCGCAGCGCACTA
TTGATCGCTTGCGGTTGCTACACGCAGTGCCGATTATTAATGAGAATGACACGGTTGCAACCTCTGAGATGCACTTTGGA
GACAACGACCGCCTTGCCGCGATTGTGTCGCACCTAGTGTCCGCGGACGCATTGGTGCTGCTCTCGGACGTAGATGGGTT
GTATGACAAAAACCCTGCGGATCCTTCGGCACGATTTGTGTCTGAAGTCAGAGACGGCAACGACCTGAAAGGTGTGATCG
CAGGCGATGGTGGGGCCGTGGGGACCGGTGGCATGGCGTCGAAGGTGTCGGCTGCGCGGTTGGCGTCGCGAAGCGGCGTG
CCCGTTTTGTTGACCTCCGCTGATTATGTGGCCGAGGCGCTGGGCGATGCTGAGGTGGGTACGGTGTTTTACCCCAAGGA
TGATCGCCTATCAGCGTGGAAGTTTTGGGCGCTGTACGCTGCAGATACTGGCGGCGCATTGCGTATCGACGCCGGCGCGG
TCGCCGCAGTCACCGCCGGCGGAAACTCCCTGCTGGCCGTGGGAATTACCGAGGTCATTGGTGATTTCCACGCTGGTGAG
ATCGTGGAGATCCTCGGCCCTGAGGGTGAGATCATTGGCCGAGGCGAGGTGGCTTATGATTCCGCAGTGCTCATCACCAT
GCTGGGCAAACAAACCGCGCAGCTGCCCGAGGGTCTGCAACGTCCCGTCGTCCATGCTGATTATCTTTCCGATTACGCGT
CACGTGCGTAG

Upstream 100 bases:

>100_bases
TGTGGATCGTGGGGCTCGTGCTCTTTTGGGTGTGGATTCCCTTGGTCTTTGGGCTAGGAACATTGTTGCTGGGGCCTTAA
AGCCTCTACCATGGCAAGAT

Downstream 100 bases:

>100_bases
AGCTTTAGGTACGGTAAATGCTTATGAATAACGCCGATGTACGGTCCCAAGAACGCAAGCAGGTCATGGTATGTGCACGT
GCCGCCCATCGTGTAGCGCC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MNAQHSSADLRDVIRNAKRIVVKIGSSSLTGENFEVSPGRIDRIVEALEDRMHRGSDVIVVSSGAVAAGMAPLGLTSRPK
DLATKQAAASVGQVHLANAWGNSFARYNRTIGQVLLTASDAGQRDRARNAQRTIDRLRLLHAVPIINENDTVATSEMHFG
DNDRLAAIVSHLVSADALVLLSDVDGLYDKNPADPSARFVSEVRDGNDLKGVIAGDGGAVGTGGMASKVSAARLASRSGV
PVLLTSADYVAEALGDAEVGTVFYPKDDRLSAWKFWALYAADTGGALRIDAGAVAAVTAGGNSLLAVGITEVIGDFHAGE
IVEILGPEGEIIGRGEVAYDSAVLITMLGKQTAQLPEGLQRPVVHADYLSDYASRA

Sequences:

>Translated_376_residues
MNAQHSSADLRDVIRNAKRIVVKIGSSSLTGENFEVSPGRIDRIVEALEDRMHRGSDVIVVSSGAVAAGMAPLGLTSRPK
DLATKQAAASVGQVHLANAWGNSFARYNRTIGQVLLTASDAGQRDRARNAQRTIDRLRLLHAVPIINENDTVATSEMHFG
DNDRLAAIVSHLVSADALVLLSDVDGLYDKNPADPSARFVSEVRDGNDLKGVIAGDGGAVGTGGMASKVSAARLASRSGV
PVLLTSADYVAEALGDAEVGTVFYPKDDRLSAWKFWALYAADTGGALRIDAGAVAAVTAGGNSLLAVGITEVIGDFHAGE
IVEILGPEGEIIGRGEVAYDSAVLITMLGKQTAQLPEGLQRPVVHADYLSDYASRA
>Mature_376_residues
MNAQHSSADLRDVIRNAKRIVVKIGSSSLTGENFEVSPGRIDRIVEALEDRMHRGSDVIVVSSGAVAAGMAPLGLTSRPK
DLATKQAAASVGQVHLANAWGNSFARYNRTIGQVLLTASDAGQRDRARNAQRTIDRLRLLHAVPIINENDTVATSEMHFG
DNDRLAAIVSHLVSADALVLLSDVDGLYDKNPADPSARFVSEVRDGNDLKGVIAGDGGAVGTGGMASKVSAARLASRSGV
PVLLTSADYVAEALGDAEVGTVFYPKDDRLSAWKFWALYAADTGGALRIDAGAVAAVTAGGNSLLAVGITEVIGDFHAGE
IVEILGPEGEIIGRGEVAYDSAVLITMLGKQTAQLPEGLQRPVVHADYLSDYASRA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=289, Percent_Identity=31.4878892733564, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI21361368, Length=291, Percent_Identity=30.9278350515464, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786437, Length=347, Percent_Identity=36.0230547550432, Blast_Score=203, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17569967, Length=279, Percent_Identity=34.4086021505376, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17569969, Length=279, Percent_Identity=34.4086021505376, Blast_Score=136, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320506, Length=398, Percent_Identity=35.1758793969849, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6321822, Length=366, Percent_Identity=35.5191256830601, Blast_Score=178, Evalue=1e-45,
Organism=Drosophila melanogaster, GI21357643, Length=356, Percent_Identity=31.7415730337079, Blast_Score=128, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_CORDI (Q6NFV9)

Other databases:

- EMBL:   BX248359
- RefSeq:   NP_940115.1
- ProteinModelPortal:   Q6NFV9
- SMR:   Q6NFV9
- GeneID:   2649402
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1777
- NMPDR:   fig|257309.1.peg.1706
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q6NFV9
- ProtClustDB:   PRK05429
- BioCyc:   CDIP257309:DIP1777-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39362; Mature: 39362

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAQHSSADLRDVIRNAKRIVVKIGSSSLTGENFEVSPGRIDRIVEALEDRMHRGSDVIV
CCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEE
VSSGAVAAGMAPLGLTSRPKDLATKQAAASVGQVHLANAWGNSFARYNRTIGQVLLTASD
EECCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHEEEECCC
AGQRDRARNAQRTIDRLRLLHAVPIINENDTVATSEMHFGDNDRLAAIVSHLVSADALVL
CCCHHHHHHHHHHHHHHHHHHHCCEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEE
LSDVDGLYDKNPADPSARFVSEVRDGNDLKGVIAGDGGAVGTGGMASKVSAARLASRSGV
EECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
PVLLTSADYVAEALGDAEVGTVFYPKDDRLSAWKFWALYAADTGGALRIDAGAVAAVTAG
EEEEECHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEEEECCCCEEEECCCCEEEEECC
GNSLLAVGITEVIGDFHAGEIVEILGPEGEIIGRGEVAYDSAVLITMLGKQTAQLPEGLQ
CCCEEEEEHHHHHCCCCCCCEEEEECCCCCEEECCCCCCCCEEEEHHHCCHHHHHHHHHH
RPVVHADYLSDYASRA
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNAQHSSADLRDVIRNAKRIVVKIGSSSLTGENFEVSPGRIDRIVEALEDRMHRGSDVIV
CCCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEE
VSSGAVAAGMAPLGLTSRPKDLATKQAAASVGQVHLANAWGNSFARYNRTIGQVLLTASD
EECCCHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHEEEECCC
AGQRDRARNAQRTIDRLRLLHAVPIINENDTVATSEMHFGDNDRLAAIVSHLVSADALVL
CCCHHHHHHHHHHHHHHHHHHHCCEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEE
LSDVDGLYDKNPADPSARFVSEVRDGNDLKGVIAGDGGAVGTGGMASKVSAARLASRSGV
EECCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCC
PVLLTSADYVAEALGDAEVGTVFYPKDDRLSAWKFWALYAADTGGALRIDAGAVAAVTAG
EEEEECHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEEEECCCCEEEECCCCEEEEECC
GNSLLAVGITEVIGDFHAGEIVEILGPEGEIIGRGEVAYDSAVLITMLGKQTAQLPEGLQ
CCCEEEEEHHHHHCCCCCCCEEEEECCCCCEEECCCCCCCCEEEEHHHCCHHHHHHHHHH
RPVVHADYLSDYASRA
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910