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The map label for this gene is cobD [H]

Identifier: 38234256

GI number: 38234256

Start: 1721486

End: 1722388

Strand: Reverse

Name: cobD [H]

Synonym: DIP1685

Alternate gene names: 38234256

Gene position: 1722388-1721486 (Counterclockwise)

Preceding gene: 38234257

Following gene: 38234252

Centisome position: 69.21

GC content: 57.59

Gene sequence:

>903_bases
ATGCTCGCAGGTCTTGTATCCGGAGTAGTACTCGATCGTATCGTTGGTGATCCAGGGGGATCGCTGCATCCTGTTGCGGT
GTTTGGCCGATGGGCTACGTGGGTGGAAAAGAAAATCTATGCGCCATCGAAACTCCGTGGTGCGATCTACGTTGCCGTTA
CTGTGGCGCCACCGACCGCTGCCGCTGTGATGATAGGCAAGAAATATCCCAATGCGGCTTTAGCTGCAAGCTTGGTGGCA
GCTATTGGCGGCAGCACACTGGAAAATACCGGAATCCGAATGGCACGTGCCTTAGAACGCGATGATATAGAAGCTGCGAG
GGATCTGGTGCCGTGGTTGTGCTCACGGGACCCAGAGTATTTGGATGAATCAGGCATAGTGCGTGCCACCGTCGAATCAT
TGGCAGAAAATACGTCAGACTCCACGATCGCTCCCGTCGTGTGGGCGGCGTTAGGTGCTGGTGGGGTAGTGGCACATCGT
TGTGTCAACACCCTTGACGCCATGGTTGGATACAAAAACGACCGTTATTCTGAATTCGGGTGGGCTGCTGCCAAACTTGA
TGACGCCATGGCGTGGGTGCCAGCACGGCTGACTGCGGTGGCGCATGTGGGTGCGGCAGCAATGTGGGGGCGTGAGCGCG
CCGCTCTACGCGCTTGGCGTGTCGACGCACCCCATCACCCGAGCCCGAACGCAGGACCAGTAGAAGCGACGGCAGCTGCT
GCACTAGGGGTCACATTAGGTGGCGAGACTCGCTACTCCTATGGCATAGAAAAACGACCGACACTAGGTGTCGGCCCGTC
GCCAAGCGTGGCGACGATACATCAGGCAGTTCGGTTATCGCGCGGTGTACAAGCTGCAGCGACAGTTGCTATCGCCGGAT
TGATGCTGTGGCGACGTTACTGA

Upstream 100 bases:

>100_bases
CAGCAGGTCGGCGGTTCGATCCCGTCAGGGCGCACAATGGTTACAAGAGCACCTCCTCACACATTGTGGGGAGGTGCTTT
TCGTATTTGGGAGAGTGATT

Downstream 100 bases:

>100_bases
TTATGCCGTGAGCTACCGTAACGATTACGCATTTTTGTTGCCACTCTATCTTCTGGAGTTTCCGGTTCAGCCGCAGCGGC
AAGCTGGGCCATGTGCTCTT

Product: putative cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MLAGLVSGVVLDRIVGDPGGSLHPVAVFGRWATWVEKKIYAPSKLRGAIYVAVTVAPPTAAAVMIGKKYPNAALAASLVA
AIGGSTLENTGIRMARALERDDIEAARDLVPWLCSRDPEYLDESGIVRATVESLAENTSDSTIAPVVWAALGAGGVVAHR
CVNTLDAMVGYKNDRYSEFGWAAAKLDDAMAWVPARLTAVAHVGAAAMWGRERAALRAWRVDAPHHPSPNAGPVEATAAA
ALGVTLGGETRYSYGIEKRPTLGVGPSPSVATIHQAVRLSRGVQAAATVAIAGLMLWRRY

Sequences:

>Translated_300_residues
MLAGLVSGVVLDRIVGDPGGSLHPVAVFGRWATWVEKKIYAPSKLRGAIYVAVTVAPPTAAAVMIGKKYPNAALAASLVA
AIGGSTLENTGIRMARALERDDIEAARDLVPWLCSRDPEYLDESGIVRATVESLAENTSDSTIAPVVWAALGAGGVVAHR
CVNTLDAMVGYKNDRYSEFGWAAAKLDDAMAWVPARLTAVAHVGAAAMWGRERAALRAWRVDAPHHPSPNAGPVEATAAA
ALGVTLGGETRYSYGIEKRPTLGVGPSPSVATIHQAVRLSRGVQAAATVAIAGLMLWRRY
>Mature_300_residues
MLAGLVSGVVLDRIVGDPGGSLHPVAVFGRWATWVEKKIYAPSKLRGAIYVAVTVAPPTAAAVMIGKKYPNAALAASLVA
AIGGSTLENTGIRMARALERDDIEAARDLVPWLCSRDPEYLDESGIVRATVESLAENTSDSTIAPVVWAALGAGGVVAHR
CVNTLDAMVGYKNDRYSEFGWAAAKLDDAMAWVPARLTAVAHVGAAAMWGRERAALRAWRVDAPHHPSPNAGPVEATAAA
ALGVTLGGETRYSYGIEKRPTLGVGPSPSVATIHQAVRLSRGVQAAATVAIAGLMLWRRY

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 31467; Mature: 31467

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAGLVSGVVLDRIVGDPGGSLHPVAVFGRWATWVEKKIYAPSKLRGAIYVAVTVAPPTA
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEEECCCCE
AAVMIGKKYPNAALAASLVAAIGGSTLENTGIRMARALERDDIEAARDLVPWLCSRDPEY
EEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LDESGIVRATVESLAENTSDSTIAPVVWAALGAGGVVAHRCVNTLDAMVGYKNDRYSEFG
HCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
WAAAKLDDAMAWVPARLTAVAHVGAAAMWGRERAALRAWRVDAPHHPSPNAGPVEATAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHH
ALGVTLGGETRYSYGIEKRPTLGVGPSPSVATIHQAVRLSRGVQAAATVAIAGLMLWRRY
HHHEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLAGLVSGVVLDRIVGDPGGSLHPVAVFGRWATWVEKKIYAPSKLRGAIYVAVTVAPPTA
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEEECCCCE
AAVMIGKKYPNAALAASLVAAIGGSTLENTGIRMARALERDDIEAARDLVPWLCSRDPEY
EEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LDESGIVRATVESLAENTSDSTIAPVVWAALGAGGVVAHRCVNTLDAMVGYKNDRYSEFG
HCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
WAAAKLDDAMAWVPARLTAVAHVGAAAMWGRERAALRAWRVDAPHHPSPNAGPVEATAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHH
ALGVTLGGETRYSYGIEKRPTLGVGPSPSVATIHQAVRLSRGVQAAATVAIAGLMLWRRY
HHHEEECCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]