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The map label for this gene is myrA [H]

Identifier: 38233583

GI number: 38233583

Start: 957691

End: 958560

Strand: Direct

Name: myrA [H]

Synonym: DIP0988

Alternate gene names: 38233583

Gene position: 957691-958560 (Clockwise)

Preceding gene: 38233582

Following gene: 161598676

Centisome position: 38.48

GC content: 55.06

Gene sequence:

>870_bases
GTGCTGTCGCATGTTGTAGATGTTCTTGCGGATCCCATTGATGGTTCTGCGTTGACGCTTGTCGACGATAACCGCCGTCT
GGTGTCTGAATCCGGCCATAGTTTTGATATTGCACGTCAGGGGTATGTGACCCTCGCTGGCGGTGCAGGTATTCGATACG
TTGGCGATGATTCTTCTATGATCCACGCCCGCGAGACGTTCCTTTCGGGTGGCCATTTCGCGCCATTCGTCGAGGCCGTG
AGTAATTCCGTTCACCTCGCCTTGGATGAGGCTAATGTTCCGGATGATGCCTCTCCTGTGATTTGTGAGGTCGGTGCCGG
CACTGGCTACTACTTGGCACATGCACTGGATGACATTGAGAACTCTCGCGGCATTGGCATTGACGTTTCTGTGCCAGCGG
CGAAGATGTTGAGCAAGTGCCATCCACGCGTGGGCGCTGTCGTAGCAGATGCATGGTCGCGATTGCCGCTTCGCGACGCC
TCTATCGATGCCATTACCGTTGTTTTTGCACCTCGTAACGCCTCCGAGTTCGCCCGTGTGCTCAAACCTGGCGGCCAAGT
TATCGTATTGACAGCCGACGCAGGTCACCTCGAAGAACTGCGCGAGCCTCTGGGCATTATCGGCGTGGAAAAGGGCAAAG
TCCAACGCATGATCGATCAAGCAGCAGGCAACCTTGTGCCTGTTTCTGATCCAGAACCCATTGAGTTCACCATGCACTTA
GACCAAGACTCCATCGCCTCCCAGATCGGTATGAGCCCCTCAGCTCGCCATATTCACCCCGATGTACTAGGAGAGAGGAT
TGCCACCCTTCCTAGTGTCATGGACGTGACCGCGCGTGCGATGATCACACGTTTTGCCAAGATCGATTAG

Upstream 100 bases:

>100_bases
GCCGCTTGGTCATCGAGATGAATAAAGAAGAGGCAGCAGAGCTGGGTGCTTTGCTGACAGAAGTTTCGCAGTAGTTTTGC
ACAATAAGGAGTAGCCCTAG

Downstream 100 bases:

>100_bases
TTCTTAACCAAAAAGATGGCGACGGGAATTATTCCCGTCGCCATCTTTTATGTTCGCTTAGCCAAGTGGCTTTGTCACGT
CGCTGCGGAGGATATCAGCG

Product: putative macrolide resistance protein

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MLSHVVDVLADPIDGSALTLVDDNRRLVSESGHSFDIARQGYVTLAGGAGIRYVGDDSSMIHARETFLSGGHFAPFVEAV
SNSVHLALDEANVPDDASPVICEVGAGTGYYLAHALDDIENSRGIGIDVSVPAAKMLSKCHPRVGAVVADAWSRLPLRDA
SIDAITVVFAPRNASEFARVLKPGGQVIVLTADAGHLEELREPLGIIGVEKGKVQRMIDQAAGNLVPVSDPEPIEFTMHL
DQDSIASQIGMSPSARHIHPDVLGERIATLPSVMDVTARAMITRFAKID

Sequences:

>Translated_289_residues
MLSHVVDVLADPIDGSALTLVDDNRRLVSESGHSFDIARQGYVTLAGGAGIRYVGDDSSMIHARETFLSGGHFAPFVEAV
SNSVHLALDEANVPDDASPVICEVGAGTGYYLAHALDDIENSRGIGIDVSVPAAKMLSKCHPRVGAVVADAWSRLPLRDA
SIDAITVVFAPRNASEFARVLKPGGQVIVLTADAGHLEELREPLGIIGVEKGKVQRMIDQAAGNLVPVSDPEPIEFTMHL
DQDSIASQIGMSPSARHIHPDVLGERIATLPSVMDVTARAMITRFAKID
>Mature_289_residues
MLSHVVDVLADPIDGSALTLVDDNRRLVSESGHSFDIARQGYVTLAGGAGIRYVGDDSSMIHARETFLSGGHFAPFVEAV
SNSVHLALDEANVPDDASPVICEVGAGTGYYLAHALDDIENSRGIGIDVSVPAAKMLSKCHPRVGAVVADAWSRLPLRDA
SIDAITVVFAPRNASEFARVLKPGGQVIVLTADAGHLEELREPLGIIGVEKGKVQRMIDQAAGNLVPVSDPEPIEFTMHL
DQDSIASQIGMSPSARHIHPDVLGERIATLPSVMDVTARAMITRFAKID

Specific function: Confers strong resistance to mycinamicin (MM) and tylosin (TY). May function as methyltransferase [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=246, Percent_Identity=32.9268292682927, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 30668; Mature: 30668

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSHVVDVLADPIDGSALTLVDDNRRLVSESGHSFDIARQGYVTLAGGAGIRYVGDDSSM
CHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCCEEEEECCCEEEEECCCCEEEECCCCCE
IHARETFLSGGHFAPFVEAVSNSVHLALDEANVPDDASPVICEVGAGTGYYLAHALDDIE
EHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHC
NSRGIGIDVSVPAAKMLSKCHPRVGAVVADAWSRLPLRDASIDAITVVFAPRNASEFARV
CCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHH
LKPGGQVIVLTADAGHLEELREPLGIIGVEKGKVQRMIDQAAGNLVPVSDPEPIEFTMHL
HCCCCCEEEEEECCCHHHHHHCCCCEEECCHHHHHHHHHHHCCCEEECCCCCCEEEEEEC
DQDSIASQIGMSPSARHIHPDVLGERIATLPSVMDVTARAMITRFAKID
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSHVVDVLADPIDGSALTLVDDNRRLVSESGHSFDIARQGYVTLAGGAGIRYVGDDSSM
CHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCCEEEEECCCEEEEECCCCEEEECCCCCE
IHARETFLSGGHFAPFVEAVSNSVHLALDEANVPDDASPVICEVGAGTGYYLAHALDDIE
EHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHC
NSRGIGIDVSVPAAKMLSKCHPRVGAVVADAWSRLPLRDASIDAITVVFAPRNASEFARV
CCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHH
LKPGGQVIVLTADAGHLEELREPLGIIGVEKGKVQRMIDQAAGNLVPVSDPEPIEFTMHL
HCCCCCEEEEEECCCHHHHHHCCCCEEECCHHHHHHHHHHHCCCEEECCCCCCEEEEEEC
DQDSIASQIGMSPSARHIHPDVLGERIATLPSVMDVTARAMITRFAKID
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8163173 [H]