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The map label for this gene is ispE

Identifier: 38233473

GI number: 38233473

Start: 850987

End: 851922

Strand: Direct

Name: ispE

Synonym: DIP0876

Alternate gene names: 38233473

Gene position: 850987-851922 (Clockwise)

Preceding gene: 38233472

Following gene: 38233474

Centisome position: 34.19

GC content: 58.87

Gene sequence:

>936_bases
ATGACAAGTTACGCCATCACAGCGGTCGCCTCGTCAAAAGTCAACCTTCATCTTGGAGTCGGCAACGCGCGTGACGACGG
CTACCATGAGCTCGTCACCGTATTTCAATCACTGAGCCTGCACGACACCATCACGGTGACACCAGCCGCAGATGATATGC
ACGATGCCGAGGACCAAGGAATCGTCGCAACGCTGAGCGTGAGCGGCGACAGTGCCCAAGCCGTGCCTACCGACGCCACC
AACCTCGCATGGCAGGCAGCCGAGAAAATGTATCAGGCACATCGTCGCAATGGTGGAGCCCCCGCCAAGCGCGTGCACAT
CGCCATCGACAAAGGGATTCCGGTCGCAGGCGGAATGGCCGGTGGATCGGCCGATGCCGCTGCCACGTTAGCAGCCATGG
CACAGTACTTGGGCAACACCAACACCGAGCAAGAAATCTTGAGCATTGCAGCCGAGCTGGGCTCCGACGTGCCCTTTACC
TACCTCGGTGATACTCGGCTAGGAACAGGCCGCGGTGAACAACTCGTCCCAGTCTTATCGCGGGGAACCTACCATTGGGC
ACTGGCGTTTTCTGCGCAAGGCCTATCTACACCCGAGGTATTCCGCAAAATCGATCAGATGGACCGCCAACCGCATCTCG
ATGTCACCGCGCTGAACGCAGCGCTGATTACGGGAGATCCCCATAAAGTAGCAGCGCATTTGCACAATGATCTCCAAGCA
GCGGCGCTTTCACTACGCCCCGAATTACGCACCATCCTAGAACAAGGTCGTGCCGCTGGAGCACTGGCCGGAATCGTATC
GGGATCCGGACCCACCTGTGCCTTCCTTTGCGAAGATGCCGAAACTGCCCAAGCAGTAGCCGCGGAATTAAGCGTGCACT
ACCGAACCGCTGTCGCTACCGGACCTGTCCGCGGCGCCCATGTGAAAGGACACTAG

Upstream 100 bases:

>100_bases
CGCTGCGCACCGCGGGAATCGACCCCATCCTCCGTGGGGAAAAACTCGATATTGCTGACTTCGTTCGTCTTGCCCGCGTC
ACCGCCGCCCAGGAGCAGGC

Downstream 100 bases:

>100_bases
TGGCCAACCTCATCAACCTAGAAAACGTCTCCAAGTCTTTCGGCCTGACAACGCTTCTCGACTCGGTGAGCTTAGGTGTT
CAAACCGGCGACCGCATCGG

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MTSYAITAVASSKVNLHLGVGNARDDGYHELVTVFQSLSLHDTITVTPAADDMHDAEDQGIVATLSVSGDSAQAVPTDAT
NLAWQAAEKMYQAHRRNGGAPAKRVHIAIDKGIPVAGGMAGGSADAAATLAAMAQYLGNTNTEQEILSIAAELGSDVPFT
YLGDTRLGTGRGEQLVPVLSRGTYHWALAFSAQGLSTPEVFRKIDQMDRQPHLDVTALNAALITGDPHKVAAHLHNDLQA
AALSLRPELRTILEQGRAAGALAGIVSGSGPTCAFLCEDAETAQAVAAELSVHYRTAVATGPVRGAHVKGH

Sequences:

>Translated_311_residues
MTSYAITAVASSKVNLHLGVGNARDDGYHELVTVFQSLSLHDTITVTPAADDMHDAEDQGIVATLSVSGDSAQAVPTDAT
NLAWQAAEKMYQAHRRNGGAPAKRVHIAIDKGIPVAGGMAGGSADAAATLAAMAQYLGNTNTEQEILSIAAELGSDVPFT
YLGDTRLGTGRGEQLVPVLSRGTYHWALAFSAQGLSTPEVFRKIDQMDRQPHLDVTALNAALITGDPHKVAAHLHNDLQA
AALSLRPELRTILEQGRAAGALAGIVSGSGPTCAFLCEDAETAQAVAAELSVHYRTAVATGPVRGAHVKGH
>Mature_310_residues
TSYAITAVASSKVNLHLGVGNARDDGYHELVTVFQSLSLHDTITVTPAADDMHDAEDQGIVATLSVSGDSAQAVPTDATN
LAWQAAEKMYQAHRRNGGAPAKRVHIAIDKGIPVAGGMAGGSADAAATLAAMAQYLGNTNTEQEILSIAAELGSDVPFTY
LGDTRLGTGRGEQLVPVLSRGTYHWALAFSAQGLSTPEVFRKIDQMDRQPHLDVTALNAALITGDPHKVAAHLHNDLQAA
ALSLRPELRTILEQGRAAGALAGIVSGSGPTCAFLCEDAETAQAVAAELSVHYRTAVATGPVRGAHVKGH

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=233, Percent_Identity=27.4678111587983, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_CORDI (Q6NIA1)

Other databases:

- EMBL:   BX248356
- RefSeq:   NP_939240.1
- ProteinModelPortal:   Q6NIA1
- SMR:   Q6NIA1
- GeneID:   2648490
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0876
- NMPDR:   fig|257309.1.peg.831
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- ProtClustDB:   PRK03188
- BioCyc:   CDIP257309:DIP0876-MONOMER
- BRENDA:   2.7.1.148
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32339; Mature: 32207

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: ACT_SITE 13-13 ACT_SITE 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSYAITAVASSKVNLHLGVGNARDDGYHELVTVFQSLSLHDTITVTPAADDMHDAEDQG
CCCEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
IVATLSVSGDSAQAVPTDATNLAWQAAEKMYQAHRRNGGAPAKRVHIAIDKGIPVAGGMA
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCC
GGSADAAATLAAMAQYLGNTNTEQEILSIAAELGSDVPFTYLGDTRLGTGRGEQLVPVLS
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHH
RGTYHWALAFSAQGLSTPEVFRKIDQMDRQPHLDVTALNAALITGDPHKVAAHLHNDLQA
CCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHH
AALSLRPELRTILEQGRAAGALAGIVSGSGPTCAFLCEDAETAQAVAAELSVHYRTAVAT
HHHHCCHHHHHHHHCCCCCHHHHEEECCCCCEEEEEECCCHHHHHHHHHHHHHEEEEEEC
GPVRGAHVKGH
CCCCCCCCCCC
>Mature Secondary Structure 
TSYAITAVASSKVNLHLGVGNARDDGYHELVTVFQSLSLHDTITVTPAADDMHDAEDQG
CCEEEEEEECCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
IVATLSVSGDSAQAVPTDATNLAWQAAEKMYQAHRRNGGAPAKRVHIAIDKGIPVAGGMA
EEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCC
GGSADAAATLAAMAQYLGNTNTEQEILSIAAELGSDVPFTYLGDTRLGTGRGEQLVPVLS
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHHH
RGTYHWALAFSAQGLSTPEVFRKIDQMDRQPHLDVTALNAALITGDPHKVAAHLHNDLQA
CCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHH
AALSLRPELRTILEQGRAAGALAGIVSGSGPTCAFLCEDAETAQAVAAELSVHYRTAVAT
HHHHCCHHHHHHHHCCCCCHHHHEEECCCCCEEEEEECCCHHHHHHHHHHHHHEEEEEEC
GPVRGAHVKGH
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910