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The map label for this gene is ksgA

Identifier: 38233472

GI number: 38233472

Start: 850103

End: 850990

Strand: Direct

Name: ksgA

Synonym: DIP0875

Alternate gene names: 38233472

Gene position: 850103-850990 (Clockwise)

Preceding gene: 38233471

Following gene: 38233473

Centisome position: 34.16

GC content: 55.86

Gene sequence:

>888_bases
ATGGAAGATCAGGCATCTGCGCAGTTATTGGGACCGGTGGAAATTCGTCAGTTGGCGGAAAAGCTCGACGTTACTCCCAC
CAAAAAACTGGGACAAAACTTTGTCCACGATCCAAACACGGTCAGAATGATCGTTTCTGCAGCCGACCTTAACTCCGATG
ATCATGTCATCGAAGTGGGGCCAGGACTTGGATCTTTAACTCTTGCCTTGCTCGATACGGCTCAAAAAGTCACCGCGGTC
GAAATTGATCCCCGCCTTGCACAGCAGCTACCACTGACTGTCGCAGAACGCGCCGGCCAGTTTGCTGACCGACTCAATCT
GATCCACAAAGACGCCCTTACTGTTGCGCCGGATGACATCGATCACCCGACAGCCCTAGTGGCAAACCTGCCCTATAACG
TGTCGGTGCCGGTGCTGCTTCACCTATTGCAGATTTTCCCTACCATTCGCCGCGTGCTGGTGATGGTGCAAGCGGAAGTG
GCAGATCGTCTTGCCGCAGACCCCGGCAACAAGGTTTATGGCGTTCCGAGCGTAAAAGCCTCCTTCTACGGAAACGTCCG
TCGCGCTGGTTCCATCGGAAAGAACGTCTTTTGGCCAGCACCCAAGATCGAATCCGGCCTAGTTCGCATCGATGTCTTTG
ATCCTGAACACCAACCGTGGCCAGTGACCGATGACATGCGTAAAGCTGTGTTCCCGCTGATCGACTCAGCCTTTGCACAA
CGACGCAAGACTCTTCGTGCAGCACTCAGCGGGCACTTTGGCTCAGGGCCGGCTGCAGAGGAAGCGCTGCGCACCGCGGG
AATCGACCCCATCCTCCGTGGGGAAAAACTCGATATTGCTGACTTCGTTCGTCTTGCCCGCGTCACCGCCGCCCAGGAGC
AGGCATGA

Upstream 100 bases:

>100_bases
GGGCTGGGGTGCTTGGCCGGCATGTACAGCAAAGCTCGGATTGCGCTAGTTTCGCCCTCAACTACTGTCGCCATGCTTGC
GCAGGGTAGATTTATTTCTC

Downstream 100 bases:

>100_bases
CAAGTTACGCCATCACAGCGGTCGCCTCGTCAAAAGTCAACCTTCATCTTGGAGTCGGCAACGCGCGTGACGACGGCTAC
CATGAGCTCGTCACCGTATT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MEDQASAQLLGPVEIRQLAEKLDVTPTKKLGQNFVHDPNTVRMIVSAADLNSDDHVIEVGPGLGSLTLALLDTAQKVTAV
EIDPRLAQQLPLTVAERAGQFADRLNLIHKDALTVAPDDIDHPTALVANLPYNVSVPVLLHLLQIFPTIRRVLVMVQAEV
ADRLAADPGNKVYGVPSVKASFYGNVRRAGSIGKNVFWPAPKIESGLVRIDVFDPEHQPWPVTDDMRKAVFPLIDSAFAQ
RRKTLRAALSGHFGSGPAAEEALRTAGIDPILRGEKLDIADFVRLARVTAAQEQA

Sequences:

>Translated_295_residues
MEDQASAQLLGPVEIRQLAEKLDVTPTKKLGQNFVHDPNTVRMIVSAADLNSDDHVIEVGPGLGSLTLALLDTAQKVTAV
EIDPRLAQQLPLTVAERAGQFADRLNLIHKDALTVAPDDIDHPTALVANLPYNVSVPVLLHLLQIFPTIRRVLVMVQAEV
ADRLAADPGNKVYGVPSVKASFYGNVRRAGSIGKNVFWPAPKIESGLVRIDVFDPEHQPWPVTDDMRKAVFPLIDSAFAQ
RRKTLRAALSGHFGSGPAAEEALRTAGIDPILRGEKLDIADFVRLARVTAAQEQA
>Mature_295_residues
MEDQASAQLLGPVEIRQLAEKLDVTPTKKLGQNFVHDPNTVRMIVSAADLNSDDHVIEVGPGLGSLTLALLDTAQKVTAV
EIDPRLAQQLPLTVAERAGQFADRLNLIHKDALTVAPDDIDHPTALVANLPYNVSVPVLLHLLQIFPTIRRVLVMVQAEV
ADRLAADPGNKVYGVPSVKASFYGNVRRAGSIGKNVFWPAPKIESGLVRIDVFDPEHQPWPVTDDMRKAVFPLIDSAFAQ
RRKTLRAALSGHFGSGPAAEEALRTAGIDPILRGEKLDIADFVRLARVTAAQEQA

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=280, Percent_Identity=32.1428571428571, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI156415992, Length=299, Percent_Identity=30.1003344481605, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI1786236, Length=267, Percent_Identity=32.5842696629214, Blast_Score=137, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI25146882, Length=217, Percent_Identity=35.0230414746544, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI25141369, Length=313, Percent_Identity=26.517571884984, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324989, Length=206, Percent_Identity=32.5242718446602, Blast_Score=109, Evalue=6e-25,
Organism=Drosophila melanogaster, GI21358017, Length=231, Percent_Identity=33.7662337662338, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21357273, Length=295, Percent_Identity=26.1016949152542, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_CORDI (Q6NIA2)

Other databases:

- EMBL:   BX248356
- RefSeq:   NP_939239.1
- ProteinModelPortal:   Q6NIA2
- SMR:   Q6NIA2
- GeneID:   2648489
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0875
- NMPDR:   fig|257309.1.peg.830
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- PhylomeDB:   Q6NIA2
- ProtClustDB:   PRK00274
- BioCyc:   CDIP257309:DIP0875-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31994; Mature: 31994

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 33-33 BINDING 35-35 BINDING 60-60 BINDING 81-81 BINDING 111-111 BINDING 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDQASAQLLGPVEIRQLAEKLDVTPTKKLGQNFVHDPNTVRMIVSAADLNSDDHVIEVG
CCCCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEC
PGLGSLTLALLDTAQKVTAVEIDPRLAQQLPLTVAERAGQFADRLNLIHKDALTVAPDDI
CCCHHHHHHHHHHHHHHEEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEECCCCC
DHPTALVANLPYNVSVPVLLHLLQIFPTIRRVLVMVQAEVADRLAADPGNKVYGVPSVKA
CCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHH
SFYGNVRRAGSIGKNVFWPAPKIESGLVRIDVFDPEHQPWPVTDDMRKAVFPLIDSAFAQ
HHHCCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
RRKTLRAALSGHFGSGPAAEEALRTAGIDPILRGEKLDIADFVRLARVTAAQEQA
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEDQASAQLLGPVEIRQLAEKLDVTPTKKLGQNFVHDPNTVRMIVSAADLNSDDHVIEVG
CCCCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEC
PGLGSLTLALLDTAQKVTAVEIDPRLAQQLPLTVAERAGQFADRLNLIHKDALTVAPDDI
CCCHHHHHHHHHHHHHHEEEEECHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEECCCCC
DHPTALVANLPYNVSVPVLLHLLQIFPTIRRVLVMVQAEVADRLAADPGNKVYGVPSVKA
CCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHH
SFYGNVRRAGSIGKNVFWPAPKIESGLVRIDVFDPEHQPWPVTDDMRKAVFPLIDSAFAQ
HHHCCHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
RRKTLRAALSGHFGSGPAAEEALRTAGIDPILRGEKLDIADFVRLARVTAAQEQA
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910