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The map label for this gene is mscL [H]

Identifier: 38233456

GI number: 38233456

Start: 832222

End: 832626

Strand: Reverse

Name: mscL [H]

Synonym: DIP0859

Alternate gene names: 38233456

Gene position: 832626-832222 (Counterclockwise)

Preceding gene: 38233457

Following gene: 38233448

Centisome position: 33.46

GC content: 52.35

Gene sequence:

>405_bases
ATGCTCAAAGGGTTTAAAGACTTTATCCTACGCGGCAACGTTGTCGAACTCGCCGTCGCGGTCGTTATCGGTACTGCTTT
CACCGCTATCGTGACCGCATTTTCCGAGCACCTAATTAACCCAATGATCGCCGCACTCGGCGGTGCTGATGTTTCCGGCC
TAGGCTTTTATCTACGCTCCGGCAACGATGCTACCTTCATGGACTTCGGTGCCGTTATCACCGCTGCACTGAACTTCCTG
ATCATCGCAGCAGTTGTGTACTTTATCCTTGTTGCTCCTATGAACAAGCTCAATGAGATGGCAGCACGCCGCAAGGGTGT
TGTTGAGGAAGAAGAAGCACCAGCCTCCATCGAAGCTGAGCTGCTCGAAGAAATCCGCGACCTGCTCAAGAACCGCAACC
TGTAA

Upstream 100 bases:

>100_bases
ACCCCTGTTGAAAAGCATAACTAAATAGTTTTGCACTGGAACTGTAAAGATAATTCCGGTAGCGTAATTGGCGCACATTT
ATAGAAAGAGGTTGTAAACC

Downstream 100 bases:

>100_bases
TTATTCTGCGGACTATCTCAACCGCCTTTTTTCCTTAAACTCTCCGACACGCTCGTGAGTTTAAGGAAAGAAGGCGGTTG
TATTTTTCAGGAGCCGTAAT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MLKGFKDFILRGNVVELAVAVVIGTAFTAIVTAFSEHLINPMIAALGGADVSGLGFYLRSGNDATFMDFGAVITAALNFL
IIAAVVYFILVAPMNKLNEMAARRKGVVEEEEAPASIEAELLEEIRDLLKNRNL

Sequences:

>Translated_134_residues
MLKGFKDFILRGNVVELAVAVVIGTAFTAIVTAFSEHLINPMIAALGGADVSGLGFYLRSGNDATFMDFGAVITAALNFL
IIAAVVYFILVAPMNKLNEMAARRKGVVEEEEAPASIEAELLEEIRDLLKNRNL
>Mature_134_residues
MLKGFKDFILRGNVVELAVAVVIGTAFTAIVTAFSEHLINPMIAALGGADVSGLGFYLRSGNDATFMDFGAVITAALNFL
IIAAVVYFILVAPMNKLNEMAARRKGVVEEEEAPASIEAELLEEIRDLLKNRNL

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=138, Percent_Identity=40.5797101449275, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14430; Mature: 14430

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGFKDFILRGNVVELAVAVVIGTAFTAIVTAFSEHLINPMIAALGGADVSGLGFYLRS
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEC
GNDATFMDFGAVITAALNFLIIAAVVYFILVAPMNKLNEMAARRKGVVEEEEAPASIEAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LLEEIRDLLKNRNL
HHHHHHHHHHCCCC
>Mature Secondary Structure
MLKGFKDFILRGNVVELAVAVVIGTAFTAIVTAFSEHLINPMIAALGGADVSGLGFYLRS
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEEC
GNDATFMDFGAVITAALNFLIIAAVVYFILVAPMNKLNEMAARRKGVVEEEEAPASIEAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
LLEEIRDLLKNRNL
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA