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The map label for this gene is vhuA [H]

Identifier: 38233284

GI number: 38233284

Start: 653448

End: 654491

Strand: Direct

Name: vhuA [H]

Synonym: DIP0677

Alternate gene names: 38233284

Gene position: 653448-654491 (Clockwise)

Preceding gene: 38233282

Following gene: 38233285

Centisome position: 26.26

GC content: 55.27

Gene sequence:

>1044_bases
GTGAGTACAACACTGCGCTTGGATCAGTTCGTTGATCCCTTCGAAGCCAAGGTGGTGTACACCGATTCGGGTGGATACTT
TGATCTTTCTGGCTTGCCGCGTCTCGACCCCATGCTCGTCGGCCGTAATGTTGCTGAAGTACCAGACATCGTCAAACGAC
TCTGCGGACTATGCCCAGTCGCTCATCACCTCGCGGGGGTACGAGCACTCGACGCCCTCTGTGGTGTAGAAGTTCCCGAA
TCCGCACAACTTGTTCGATTGCTGCTTCACCACGGGTCGATTCTTTATGCTTCCCGCGATATGGACATCAAGCGCCTTGG
CAAGGCAGTAATGGCTGCTGCAGGTTCGCCTGGCCATTTCCCTGACGTTGCGATCCCCGGTGGTGTGCGTGCACTTCCAG
ATCCGCAGGCACTCGCAGAGCTGCGCCTACCGGAAAATTACGAGGAGTCATTTGAACCAGTTCCTTACGACGGTTTCGAC
ATGATGCTCACCAGTCATGGAACCCTCGACCCCCTTGGCGATCATGTCACCGCTCGTTCTGGTGAAGAACGCATCACCTT
TGATCTGCAAACCTGGGCTGACCATGTAGCAGAATCACGCCCTGGCGATCCTGCGCCTCGACCACTGGTCCATGGTCATC
CTTATCGCGTAGGCCCCTACGCTCACGGAGAAGCGATGGTGCCGCGGTCACTGGCTGAGATCCGACGTATCATCGCCGAT
CCGCGGTTGTGCGAAGGAGAATTCCGGAAAGAATCGTCGATTCTTTCCGGAATTGGAGTTGGTGCGGTTGAAGGTCCACG
GGGATTGCTAGTGCATCGTTACGTCGCTAATGAGGATGGTGTGTTGGTGGATTGTCAGATTTTGACTCCGACTGCTCAAA
ATGAGGCGTGGTTGGCGTCGATGTTGGAGGCGTCGTTGCAACAGGATTCGGATAGGGGAGTAGAACTGAGCGTGGAGAAT
TCGATTAGGACGGCGAATCCATGTTTGCCGTGTAGTTCGGCTCCGGAGGGGCATATGGGCGTTGTGATTGAGAAAGGTAA
CTAG

Upstream 100 bases:

>100_bases
CGTCGATTCTTTCCGGAAGGAATCGACGATTCCGTCGCAGACACCACACCAGATAACAGTCCAAGTTCATGCATATAACC
ATTATGAGGAGCTATAACCA

Downstream 100 bases:

>100_bases
AGCAGATGTGTCTTGGTATTCCAGCGCGTGTTGTTGAGGTTCTTGATGGCCCATTGCCGATGGCGGTGGTGGATATGGCT
GGTGAGCAGCGGCCGTGTTC

Product: putative reducing hydrogenase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSTTLRLDQFVDPFEAKVVYTDSGGYFDLSGLPRLDPMLVGRNVAEVPDIVKRLCGLCPVAHHLAGVRALDALCGVEVPE
SAQLVRLLLHHGSILYASRDMDIKRLGKAVMAAAGSPGHFPDVAIPGGVRALPDPQALAELRLPENYEESFEPVPYDGFD
MMLTSHGTLDPLGDHVTARSGEERITFDLQTWADHVAESRPGDPAPRPLVHGHPYRVGPYAHGEAMVPRSLAEIRRIIAD
PRLCEGEFRKESSILSGIGVGAVEGPRGLLVHRYVANEDGVLVDCQILTPTAQNEAWLASMLEASLQQDSDRGVELSVEN
SIRTANPCLPCSSAPEGHMGVVIEKGN

Sequences:

>Translated_347_residues
MSTTLRLDQFVDPFEAKVVYTDSGGYFDLSGLPRLDPMLVGRNVAEVPDIVKRLCGLCPVAHHLAGVRALDALCGVEVPE
SAQLVRLLLHHGSILYASRDMDIKRLGKAVMAAAGSPGHFPDVAIPGGVRALPDPQALAELRLPENYEESFEPVPYDGFD
MMLTSHGTLDPLGDHVTARSGEERITFDLQTWADHVAESRPGDPAPRPLVHGHPYRVGPYAHGEAMVPRSLAEIRRIIAD
PRLCEGEFRKESSILSGIGVGAVEGPRGLLVHRYVANEDGVLVDCQILTPTAQNEAWLASMLEASLQQDSDRGVELSVEN
SIRTANPCLPCSSAPEGHMGVVIEKGN
>Mature_346_residues
STTLRLDQFVDPFEAKVVYTDSGGYFDLSGLPRLDPMLVGRNVAEVPDIVKRLCGLCPVAHHLAGVRALDALCGVEVPES
AQLVRLLLHHGSILYASRDMDIKRLGKAVMAAAGSPGHFPDVAIPGGVRALPDPQALAELRLPENYEESFEPVPYDGFDM
MLTSHGTLDPLGDHVTARSGEERITFDLQTWADHVAESRPGDPAPRPLVHGHPYRVGPYAHGEAMVPRSLAEIRRIIADP
RLCEGEFRKESSILSGIGVGAVEGPRGLLVHRYVANEDGVLVDCQILTPTAQNEAWLASMLEASLQQDSDRGVELSVENS
IRTANPCLPCSSAPEGHMGVVIEKGN

Specific function: Unknown

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 37467; Mature: 37336

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTLRLDQFVDPFEAKVVYTDSGGYFDLSGLPRLDPMLVGRNVAEVPDIVKRLCGLCPV
CCCCCCHHHHCCCCCEEEEEECCCCEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHH
AHHLAGVRALDALCGVEVPESAQLVRLLLHHGSILYASRDMDIKRLGKAVMAAAGSPGHF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCC
PDVAIPGGVRALPDPQALAELRLPENYEESFEPVPYDGFDMMLTSHGTLDPLGDHVTARS
CCCCCCCCCCCCCCHHHHHHCCCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
GEERITFDLQTWADHVAESRPGDPAPRPLVHGHPYRVGPYAHGEAMVPRSLAEIRRIIAD
CCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCC
PRLCEGEFRKESSILSGIGVGAVEGPRGLLVHRYVANEDGVLVDCQILTPTAQNEAWLAS
CCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHHHHH
MLEASLQQDSDRGVELSVENSIRTANPCLPCSSAPEGHMGVVIEKGN
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
STTLRLDQFVDPFEAKVVYTDSGGYFDLSGLPRLDPMLVGRNVAEVPDIVKRLCGLCPV
CCCCCHHHHCCCCCEEEEEECCCCEEECCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHH
AHHLAGVRALDALCGVEVPESAQLVRLLLHHGSILYASRDMDIKRLGKAVMAAAGSPGHF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCC
PDVAIPGGVRALPDPQALAELRLPENYEESFEPVPYDGFDMMLTSHGTLDPLGDHVTARS
CCCCCCCCCCCCCCHHHHHHCCCCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
GEERITFDLQTWADHVAESRPGDPAPRPLVHGHPYRVGPYAHGEAMVPRSLAEIRRIIAD
CCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHCC
PRLCEGEFRKESSILSGIGVGAVEGPRGLLVHRYVANEDGVLVDCQILTPTAQNEAWLAS
CCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHHHHH
MLEASLQQDSDRGVELSVENSIRTANPCLPCSSAPEGHMGVVIEKGN
HHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]