Click here to switch to the map view.

The map label for this gene is hybD [H]

Identifier: 38233282

GI number: 38233282

Start: 652547

End: 653059

Strand: Direct

Name: hybD [H]

Synonym: DIP0675

Alternate gene names: 38233282

Gene position: 652547-653059 (Clockwise)

Preceding gene: 38233281

Following gene: 38233284

Centisome position: 26.22

GC content: 59.84

Gene sequence:

>513_bases
GTGATCACAGTAATCGGAGTAGGAAACCCAGTCATGGGAGACGACGGCGCAGGCCTCGCAGTTTTGCGCCGTCTCCAAGG
CATTACCAATGACCTTGACTGGGTTCCGCGACTATCCACATCCGACCCCGCCGCCCCCAACTACATCGACGGCGGCACCT
CCGGCATGGAATTGCTCCCCATAGTCCAAGACGCAGACAAACTTCTGCTTCTCGACGCCGTCACAGGTCCAGGCGCGCCA
GGCGATGTTGTTCGCCTCCACGGCGACCAGATCCCACGTCTGCTGAGCTCACGGCTATCCCCACACCAAGTAGGTCTGCT
CGACCTGCTTTCTGCAGCACGCCTTCTTGGCCGTGAACCCAACGAGGTAGCAGTAGTGGGAATCGTCGTCGAGCACTGCG
ACCTTCACGTAGGCCTCAGCCAAGCAGTAACAGACGCTATCGAAACCGCCACCGACGCAGCCCGAGACGTCATCGCTGAA
TGGCAACGTCTCGAAGCCGCCCACGCGAATTAA

Upstream 100 bases:

>100_bases
GAACGCCATGGAGGTGTCTCCTCTATGTTCAACGGTGCCGTATGGATGCGTCGAGGTGCGCGCCCAGTCGACGCCCCAGA
GATCGGATAACGAAAACAGC

Downstream 100 bases:

>100_bases
GGTTCTTCGTCCTTGGGATCTTCAATATCCACCCAAGCAATCTCAAACTCCCGCCCCGACCGCAAATCAGCCGTCGGGGT
TCCACACACCGGACAACACA

Product: putative hydrogenase maturation protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MITVIGVGNPVMGDDGAGLAVLRRLQGITNDLDWVPRLSTSDPAAPNYIDGGTSGMELLPIVQDADKLLLLDAVTGPGAP
GDVVRLHGDQIPRLLSSRLSPHQVGLLDLLSAARLLGREPNEVAVVGIVVEHCDLHVGLSQAVTDAIETATDAARDVIAE
WQRLEAAHAN

Sequences:

>Translated_170_residues
MITVIGVGNPVMGDDGAGLAVLRRLQGITNDLDWVPRLSTSDPAAPNYIDGGTSGMELLPIVQDADKLLLLDAVTGPGAP
GDVVRLHGDQIPRLLSSRLSPHQVGLLDLLSAARLLGREPNEVAVVGIVVEHCDLHVGLSQAVTDAIETATDAARDVIAE
WQRLEAAHAN
>Mature_170_residues
MITVIGVGNPVMGDDGAGLAVLRRLQGITNDLDWVPRLSTSDPAAPNYIDGGTSGMELLPIVQDADKLLLLDAVTGPGAP
GDVVRLHGDQIPRLLSSRLSPHQVGLLDLLSAARLLGREPNEVAVVGIVVEHCDLHVGLSQAVTDAIETATDAARDVIAE
WQRLEAAHAN

Specific function: Protease involved in the C-terminal processing of hybC, the large subunit of hydrogenase 2. Specifically cleaves off a 15 amino acid peptide from the C-terminus of the precursor of hybC [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789367, Length=152, Percent_Identity=35.5263157894737, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787209, Length=128, Percent_Identity=33.59375, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 17827; Mature: 17827

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITVIGVGNPVMGDDGAGLAVLRRLQGITNDLDWVPRLSTSDPAAPNYIDGGTSGMELLP
CEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEH
IVQDADKLLLLDAVTGPGAPGDVVRLHGDQIPRLLSSRLSPHQVGLLDLLSAARLLGREP
HHCCCCEEEEEEECCCCCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
NEVAVVGIVVEHCDLHVGLSQAVTDAIETATDAARDVIAEWQRLEAAHAN
CCEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITVIGVGNPVMGDDGAGLAVLRRLQGITNDLDWVPRLSTSDPAAPNYIDGGTSGMELLP
CEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEH
IVQDADKLLLLDAVTGPGAPGDVVRLHGDQIPRLLSSRLSPHQVGLLDLLSAARLLGREP
HHCCCCEEEEEEECCCCCCCCCEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
NEVAVVGIVVEHCDLHVGLSQAVTDAIETATDAARDVIAEWQRLEAAHAN
CCEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8021226; 9278503; 10331925 [H]