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The map label for this gene is purK [H]

Identifier: 38233270

GI number: 38233270

Start: 640046

End: 641251

Strand: Direct

Name: purK [H]

Synonym: DIP0663

Alternate gene names: 38233270

Gene position: 640046-641251 (Clockwise)

Preceding gene: 38233269

Following gene: 38233271

Centisome position: 25.72

GC content: 57.71

Gene sequence:

>1206_bases
TTGTTACCCGTGACTCACAGCAACGGTAATCCAGCAGCCCACGCGACAAACATGCCAATTCTGTGCGTGATCGGCGACGG
TCAATTGGCACGAATGATGCATACCGCAGCTATCGAGTTAGGGCAATCGGTGCGTATTCTCGCAGGAGCTCCTAATGCCT
CCGCAGCGCAGGTAGCAGCCGACGTTGTGCTGGGGGACTACACCAACCTCGACGATCTGCGCCGCGTCACCGAAGGCGCA
CACGCAGTCACCTTCGATCACGAACACGTGCCCAACCAGCACCTAGAAGCACTGATCGCAGAAGGAATCAACGTCCAACC
GCGGCCAGCTGCATTGGTCAATGCACAAGACAAACTCGTGATGCGCCAGCGGCTAACAGAACTCGGTGCCCCAGTTCCCG
CATTCGCAGCCATCGACAGCGTTGAAGACGCCACCGCATTCTGGAACACCGTTGACGGTGCCGTCTGCTTGAAAGCCCGC
CGCGGCGGCTACGACGGCAAAGGCGTGTGGTTCCCCGACACCCTCGAGGAACTTACCGAGCTCGTCTCCGAACTACTAGC
AGCCGGAACCCCACTAATGGGCGAACACAAAGTGACCCTTGTTCGTGAACTATCCGCCATGGTCGCTCGCACCCCATCCG
GAGAAGTCAAAGGCTGGCCAGTCGTAGAATCCATCCAAAAAGATGGCATCTGCTGGTTCGCTATCGCACCTGCCCCCGAG
CTCTCCCCAGAGCTGAAAGAACAAGCCGAAAACGTTGCCATCACCATTGCCACCGAACTCGGCGTGACCGGCAACCTCGC
CGTTGAACTGTTTGAAACTCCCGACGGCATCTTTGTCAACGAGCTAGCCATGCGCCCCCACAACACAGGCCACTGGACCC
AAGATGGCTGCGTGACCGACCAATTCGAGCAACACATCCGTGCTGTACTCGACCTACCGCTAGGATCGACCGCACCGCTG
CAAAACTACACCGTCATGGCAAACGTTCTCGGTGCTGCCGAAGACCCCGAAACCCCCATGCCACAGCGCATGATCGACGT
GTGGAAGCGCTACCCAGAAGCGAAAATCCACATGTATGGCAAAGACCATCGCCTAGGCCGCAAAATCGGTCACGTCAACA
TGGCCGGCACGGATGCAGAAGAACTGAAAACCGCAGCTGAAAACGCCGCCTACTACCTCGTTCACGCCGAATGGAAGGAA
CAATAA

Upstream 100 bases:

>100_bases
CTAAATATGTAGTCAACGAAATAGAGCAACATATTGGCTATCGGTAACCCCCGTATCTGGTTATAACCGGCATGGGGTGG
TGCAGGGGTGCACGCTATAG

Downstream 100 bases:

>100_bases
TGACACCACTCGTAGGCCTTATCATGGGCTCCGACTCCGACTGGCCCACCGTTGAACCAGCCGCCGAGGTACTCGCCGAA
TTCGGCATCCCCTTCGAAGT

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MLPVTHSNGNPAAHATNMPILCVIGDGQLARMMHTAAIELGQSVRILAGAPNASAAQVAADVVLGDYTNLDDLRRVTEGA
HAVTFDHEHVPNQHLEALIAEGINVQPRPAALVNAQDKLVMRQRLTELGAPVPAFAAIDSVEDATAFWNTVDGAVCLKAR
RGGYDGKGVWFPDTLEELTELVSELLAAGTPLMGEHKVTLVRELSAMVARTPSGEVKGWPVVESIQKDGICWFAIAPAPE
LSPELKEQAENVAITIATELGVTGNLAVELFETPDGIFVNELAMRPHNTGHWTQDGCVTDQFEQHIRAVLDLPLGSTAPL
QNYTVMANVLGAAEDPETPMPQRMIDVWKRYPEAKIHMYGKDHRLGRKIGHVNMAGTDAEELKTAAENAAYYLVHAEWKE
Q

Sequences:

>Translated_401_residues
MLPVTHSNGNPAAHATNMPILCVIGDGQLARMMHTAAIELGQSVRILAGAPNASAAQVAADVVLGDYTNLDDLRRVTEGA
HAVTFDHEHVPNQHLEALIAEGINVQPRPAALVNAQDKLVMRQRLTELGAPVPAFAAIDSVEDATAFWNTVDGAVCLKAR
RGGYDGKGVWFPDTLEELTELVSELLAAGTPLMGEHKVTLVRELSAMVARTPSGEVKGWPVVESIQKDGICWFAIAPAPE
LSPELKEQAENVAITIATELGVTGNLAVELFETPDGIFVNELAMRPHNTGHWTQDGCVTDQFEQHIRAVLDLPLGSTAPL
QNYTVMANVLGAAEDPETPMPQRMIDVWKRYPEAKIHMYGKDHRLGRKIGHVNMAGTDAEELKTAAENAAYYLVHAEWKE
Q
>Mature_401_residues
MLPVTHSNGNPAAHATNMPILCVIGDGQLARMMHTAAIELGQSVRILAGAPNASAAQVAADVVLGDYTNLDDLRRVTEGA
HAVTFDHEHVPNQHLEALIAEGINVQPRPAALVNAQDKLVMRQRLTELGAPVPAFAAIDSVEDATAFWNTVDGAVCLKAR
RGGYDGKGVWFPDTLEELTELVSELLAAGTPLMGEHKVTLVRELSAMVARTPSGEVKGWPVVESIQKDGICWFAIAPAPE
LSPELKEQAENVAITIATELGVTGNLAVELFETPDGIFVNELAMRPHNTGHWTQDGCVTDQFEQHIRAVLDLPLGSTAPL
QNYTVMANVLGAAEDPETPMPQRMIDVWKRYPEAKIHMYGKDHRLGRKIGHVNMAGTDAEELKTAAENAAYYLVHAEWKE
Q

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=370, Percent_Identity=28.3783783783784, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1788155, Length=302, Percent_Identity=26.8211920529801, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324702, Length=377, Percent_Identity=33.1564986737401, Blast_Score=172, Evalue=9e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 43438; Mature: 43438

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPVTHSNGNPAAHATNMPILCVIGDGQLARMMHTAAIELGQSVRILAGAPNASAAQVAA
CCCCCCCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
DVVLGDYTNLDDLRRVTEGAHAVTFDHEHVPNQHLEALIAEGINVQPRPAALVNAQDKLV
HHHCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEECCHHHHH
MRQRLTELGAPVPAFAAIDSVEDATAFWNTVDGAVCLKARRGGYDGKGVWFPDTLEELTE
HHHHHHHHCCCCCHHHHHHCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
LVSELLAAGTPLMGEHKVTLVRELSAMVARTPSGEVKGWPVVESIQKDGICWFAIAPAPE
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCEEEEEECCCCC
LSPELKEQAENVAITIATELGVTGNLAVELFETPDGIFVNELAMRPHNTGHWTQDGCVTD
CCHHHHHCCCCEEEEEEEECCCCCCEEEEEEECCCCEEEHHHHCCCCCCCCCCCCCCCHH
QFEQHIRAVLDLPLGSTAPLQNYTVMANVLGAAEDPETPMPQRMIDVWKRYPEAKIHMYG
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEC
KDHRLGRKIGHVNMAGTDAEELKTAAENAAYYLVHAEWKEQ
CCCHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCC
>Mature Secondary Structure
MLPVTHSNGNPAAHATNMPILCVIGDGQLARMMHTAAIELGQSVRILAGAPNASAAQVAA
CCCCCCCCCCCCCEECCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
DVVLGDYTNLDDLRRVTEGAHAVTFDHEHVPNQHLEALIAEGINVQPRPAALVNAQDKLV
HHHCCCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCEECCHHHHH
MRQRLTELGAPVPAFAAIDSVEDATAFWNTVDGAVCLKARRGGYDGKGVWFPDTLEELTE
HHHHHHHHCCCCCHHHHHHCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
LVSELLAAGTPLMGEHKVTLVRELSAMVARTPSGEVKGWPVVESIQKDGICWFAIAPAPE
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCEEEEEECCCCC
LSPELKEQAENVAITIATELGVTGNLAVELFETPDGIFVNELAMRPHNTGHWTQDGCVTD
CCHHHHHCCCCEEEEEEEECCCCCCEEEEEEECCCCEEEHHHHCCCCCCCCCCCCCCCHH
QFEQHIRAVLDLPLGSTAPLQNYTVMANVLGAAEDPETPMPQRMIDVWKRYPEAKIHMYG
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEC
KDHRLGRKIGHVNMAGTDAEELKTAAENAAYYLVHAEWKEQ
CCCHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8998996 [H]