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The map label for this gene is birA [H]

Identifier: 38233268

GI number: 38233268

Start: 638727

End: 639527

Strand: Direct

Name: birA [H]

Synonym: DIP0661

Alternate gene names: 38233268

Gene position: 638727-639527 (Clockwise)

Preceding gene: 38233266

Following gene: 38233269

Centisome position: 25.67

GC content: 56.3

Gene sequence:

>801_bases
GTGTCTTCTGATTCCCGCGCCCCCTTGGACATTGCTTGGCTTCACGAACAGCTCGTTGAAAACGGGCCCTACAACCGTCT
CGAGCACACGATGGCAACGGGATCGACAAACACGGATCTCGTGGCGGCAGCACATGCTGGGGCACCGGAATGGACAGCAT
TTCTTACCGAACACCAGTCACAAGGTCGCGGGCGTATGGGTCGCAAATATGAATCACCTGTGGGCGCACAGCTCACCCTT
TCCGTTCTTATCCGTCCCCGCAAAGAATCGGTTTCTCGGTTAGGTACCATGCCACTTGCCACAGGTCTCGCGCTTGTCGA
CGTCCTCGGCTCCGATCCCCGTATCGGCCTCAAATGGCCCAACGACCTGCTATGCGACGGCCGCAAGCTCTGCGGCATTT
TGGCAGAAGCTGTAAGCCTTGGTGATCACCCTGCCGTCGTAATTGGTTTAGGGCTTAATACCGCGCTGACCAAAGAAGAA
CTCCCTGTCGCCCACGCCACCAGCCTTGATCTTGAGGGCATTGCTTATGAGCGCAACCAGCTGGCCGTGGATGTGCTCAA
CGCCTTGTATAAGCGCCTAAACCAATGGATTGCGGGCGATCCCTCGCTGATGGGGGACTATCGACGCGTGTGTACGTCGA
TAGGCGCGGATGTGCGCGTCGTGTTGTCAGAAGATAAGGAACTGCTTGGAGTGGCTGAGTCTGTCACCGATGAAGGCCTT
ATTTGCGTGCGCGATGCTAGCGGTGAACTCCATGAATTTATGGTCGGTGACGTGACCCATCTGCGCCTTCAGGATAAATA
A

Upstream 100 bases:

>100_bases
GCGTGTGGAAAAATTAAAAAATTTCTCCCCAAAGGTCACCGGCAGCCGGCATACCCACCTTTTTGCGGCAACGACTATTA
TCTAAGGAATGACTTCCCCC

Downstream 100 bases:

>100_bases
GTGGCAGGAATTCGATTGCAGCAGGGGGAAGAAGTTCGCGTCGACGTCACCGCACCGCTGACACGTTTGATGCTTCCCGT
ATGTGAATTGCTCCTTATTA

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MSSDSRAPLDIAWLHEQLVENGPYNRLEHTMATGSTNTDLVAAAHAGAPEWTAFLTEHQSQGRGRMGRKYESPVGAQLTL
SVLIRPRKESVSRLGTMPLATGLALVDVLGSDPRIGLKWPNDLLCDGRKLCGILAEAVSLGDHPAVVIGLGLNTALTKEE
LPVAHATSLDLEGIAYERNQLAVDVLNALYKRLNQWIAGDPSLMGDYRRVCTSIGADVRVVLSEDKELLGVAESVTDEGL
ICVRDASGELHEFMVGDVTHLRLQDK

Sequences:

>Translated_266_residues
MSSDSRAPLDIAWLHEQLVENGPYNRLEHTMATGSTNTDLVAAAHAGAPEWTAFLTEHQSQGRGRMGRKYESPVGAQLTL
SVLIRPRKESVSRLGTMPLATGLALVDVLGSDPRIGLKWPNDLLCDGRKLCGILAEAVSLGDHPAVVIGLGLNTALTKEE
LPVAHATSLDLEGIAYERNQLAVDVLNALYKRLNQWIAGDPSLMGDYRRVCTSIGADVRVVLSEDKELLGVAESVTDEGL
ICVRDASGELHEFMVGDVTHLRLQDK
>Mature_265_residues
SSDSRAPLDIAWLHEQLVENGPYNRLEHTMATGSTNTDLVAAAHAGAPEWTAFLTEHQSQGRGRMGRKYESPVGAQLTLS
VLIRPRKESVSRLGTMPLATGLALVDVLGSDPRIGLKWPNDLLCDGRKLCGILAEAVSLGDHPAVVIGLGLNTALTKEEL
PVAHATSLDLEGIAYERNQLAVDVLNALYKRLNQWIAGDPSLMGDYRRVCTSIGADVRVVLSEDKELLGVAESVTDEGLI
CVRDASGELHEFMVGDVTHLRLQDK

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=214, Percent_Identity=31.7757009345794, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28767; Mature: 28636

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDSRAPLDIAWLHEQLVENGPYNRLEHTMATGSTNTDLVAAAHAGAPEWTAFLTEHQS
CCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
QGRGRMGRKYESPVGAQLTLSVLIRPRKESVSRLGTMPLATGLALVDVLGSDPRIGLKWP
CCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCC
NDLLCDGRKLCGILAEAVSLGDHPAVVIGLGLNTALTKEELPVAHATSLDLEGIAYERNQ
CHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCHHCCCCCCCCEEECCHH
LAVDVLNALYKRLNQWIAGDPSLMGDYRRVCTSIGADVRVVLSEDKELLGVAESVTDEGL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCE
ICVRDASGELHEFMVGDVTHLRLQDK
EEEECCCCCHHHHHHCCEEEEEEECC
>Mature Secondary Structure 
SSDSRAPLDIAWLHEQLVENGPYNRLEHTMATGSTNTDLVAAAHAGAPEWTAFLTEHQS
CCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHH
QGRGRMGRKYESPVGAQLTLSVLIRPRKESVSRLGTMPLATGLALVDVLGSDPRIGLKWP
CCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCC
NDLLCDGRKLCGILAEAVSLGDHPAVVIGLGLNTALTKEELPVAHATSLDLEGIAYERNQ
CHHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCHHCCCCCCCCEEECCHH
LAVDVLNALYKRLNQWIAGDPSLMGDYRRVCTSIGADVRVVLSEDKELLGVAESVTDEGL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCCE
ICVRDASGELHEFMVGDVTHLRLQDK
EEEECCCCCHHHHHHCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]