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The map label for this gene is guaA

Identifier: 38233202

GI number: 38233202

Start: 562428

End: 564005

Strand: Direct

Name: guaA

Synonym: DIP0595

Alternate gene names: 38233202

Gene position: 562428-564005 (Clockwise)

Preceding gene: 38233201

Following gene: 38233204

Centisome position: 22.6

GC content: 60.96

Gene sequence:

>1578_bases
GTGACGCAGCAAACTTCCCAAAAACACAATCCAGTCTTGGTTGTAGACTTTGGCGCGCAATACGCCCAGCTGATCGCTCG
GCGCGTGCGCGAAGCCAACATCTACTCCGAGGTCGTCCCGCATACCGCCACGGTTGAAGAGATCAAGGCCAAAAACCCGG
CGGCGTTAATCCTGTCCGGTGGCCCATCATCGGTTTACGCCGACGGCGCACCATCGCTTGACCCACAGGTTCTCGAACTC
GGCATCCCCGTCTTCGGCATCTGCTACGGCTTCCAAGCTATGACCCACGCCCTAGGCGGCACCGTGGCCAACACCGGCAA
CCGTGAATACGGTCGCACCGAAATGACCGTCGATGGCGGCATTCTCCACGCTGACCTCGAAGAAACCCACAAAGTATGGA
TGAGCCACGGTGACGCTGTCTCCGTTGCACCAGAGGGATTCGTTGTCACCGCCCACTCCGAAGGTGCACCCGTCGCAGCC
TTCGAATGCGCCGAAAAGCGCATGGCAGGCGTGCAATACCACCCAGAGGTTCTGCACTCGCCACACGGCCAAGAAGTCAT
GGTGCGCTTCCTCACCGAGATCGCCGGCCTTGAGCAAAACTGGACCGCAGCCAACATCGCCGAAGAGCTTATCGACGCAG
TCTGCGCACAGGTCGGACCAGAAGGCCGCGCTATCTGTGGTCTTTCCGGTGGTGTTGACTCTGCAGTCGCAGCAGCACTC
GTCCAGCGCGCCATCGGCGACCGCCTCACCTGCGTATTCGTCGACCATGGCTTGCTGCGTGCAGGCGAACGCGAACAGGT
CCAAACCGATTTCGTCGCCGCAACCGGCGCAAAACTCGTCACCGTTGACGAGCGCGAAGCATTCCTGAACAAGCTCGCAG
GTGTCACCGAACCAGAAGCTAAGCGCAAAGCCATCGGCGCAGAATTCATCCGCTCCTTCGAACGCGCAGTCGCCGGCATT
CTCGACGACGCCCCCGAAGGCTCCACCGTGGACTTTCTCGTCCAAGGCACCCTCTACCCAGACGTTGTAGAATCCGGCGG
CGGCTCTGGTACCGCCAACATCAAGAGCCACCACAACGTGGGCGGCCTGCCCGACGACGTAGAATTCCAACTCGTCGAAC
CACTACGCTTACTGTTCAAAGACGAAGTACGCGCAGTGGGTCGCGAACTCGGTCTGCCTGAAGAAATCGTCAACCGCCAG
CCCTTCCCAGGCCCCGGCCTCGGCATCCGCATCATCGGCGAAGTCACCGAAGAGCGCCTGGAAACACTACGCGCAGCCGA
CCTCATCGCCCGCACCGAGCTGACCGCAGCTGGCCTCGACAGCGAAATCTGGCAGTGCCCAGTAGTTTTGCTTGCCGACG
TCCGCTCCGTCGGCGTCCAAGGCGACGGCCGCACTTACGGACATCCCATCGTCCTACGCCCCGTCTCCTCCGAAGACGCC
ATGACCGCAGACTGGACCCGACTGCCCTACGACGTGCTCGAGAAAATCTCCACACGTATCACCAACGAGGTCTCAGACGT
CAACCGCGTCGTCCTCGACTGCACCTCCAAGCCACCAGGAACCATCGAGTGGGAATAA

Upstream 100 bases:

>100_bases
TACCGGCCATGTTGGGTAGACGGGGTGCTGGGGGCGTCGATACGCAGGAAAATGACAGACAGTGAGGGATTATGCGGGCA
CGTCAGGTAAGATTTTGGCC

Downstream 100 bases:

>100_bases
TCCCCACACAGTTCCAACACCAAGCCCCCACATATGCAGCAATTCCCACTGCACAGCGTGGGGGCTTCGTGCTTGTTAAC
CCACCGCCATTACTGACACC

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MTQQTSQKHNPVLVVDFGAQYAQLIARRVREANIYSEVVPHTATVEEIKAKNPAALILSGGPSSVYADGAPSLDPQVLEL
GIPVFGICYGFQAMTHALGGTVANTGNREYGRTEMTVDGGILHADLEETHKVWMSHGDAVSVAPEGFVVTAHSEGAPVAA
FECAEKRMAGVQYHPEVLHSPHGQEVMVRFLTEIAGLEQNWTAANIAEELIDAVCAQVGPEGRAICGLSGGVDSAVAAAL
VQRAIGDRLTCVFVDHGLLRAGEREQVQTDFVAATGAKLVTVDEREAFLNKLAGVTEPEAKRKAIGAEFIRSFERAVAGI
LDDAPEGSTVDFLVQGTLYPDVVESGGGSGTANIKSHHNVGGLPDDVEFQLVEPLRLLFKDEVRAVGRELGLPEEIVNRQ
PFPGPGLGIRIIGEVTEERLETLRAADLIARTELTAAGLDSEIWQCPVVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDA
MTADWTRLPYDVLEKISTRITNEVSDVNRVVLDCTSKPPGTIEWE

Sequences:

>Translated_525_residues
MTQQTSQKHNPVLVVDFGAQYAQLIARRVREANIYSEVVPHTATVEEIKAKNPAALILSGGPSSVYADGAPSLDPQVLEL
GIPVFGICYGFQAMTHALGGTVANTGNREYGRTEMTVDGGILHADLEETHKVWMSHGDAVSVAPEGFVVTAHSEGAPVAA
FECAEKRMAGVQYHPEVLHSPHGQEVMVRFLTEIAGLEQNWTAANIAEELIDAVCAQVGPEGRAICGLSGGVDSAVAAAL
VQRAIGDRLTCVFVDHGLLRAGEREQVQTDFVAATGAKLVTVDEREAFLNKLAGVTEPEAKRKAIGAEFIRSFERAVAGI
LDDAPEGSTVDFLVQGTLYPDVVESGGGSGTANIKSHHNVGGLPDDVEFQLVEPLRLLFKDEVRAVGRELGLPEEIVNRQ
PFPGPGLGIRIIGEVTEERLETLRAADLIARTELTAAGLDSEIWQCPVVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDA
MTADWTRLPYDVLEKISTRITNEVSDVNRVVLDCTSKPPGTIEWE
>Mature_524_residues
TQQTSQKHNPVLVVDFGAQYAQLIARRVREANIYSEVVPHTATVEEIKAKNPAALILSGGPSSVYADGAPSLDPQVLELG
IPVFGICYGFQAMTHALGGTVANTGNREYGRTEMTVDGGILHADLEETHKVWMSHGDAVSVAPEGFVVTAHSEGAPVAAF
ECAEKRMAGVQYHPEVLHSPHGQEVMVRFLTEIAGLEQNWTAANIAEELIDAVCAQVGPEGRAICGLSGGVDSAVAAALV
QRAIGDRLTCVFVDHGLLRAGEREQVQTDFVAATGAKLVTVDEREAFLNKLAGVTEPEAKRKAIGAEFIRSFERAVAGIL
DDAPEGSTVDFLVQGTLYPDVVESGGGSGTANIKSHHNVGGLPDDVEFQLVEPLRLLFKDEVRAVGRELGLPEEIVNRQP
FPGPGLGIRIIGEVTEERLETLRAADLIARTELTAAGLDSEIWQCPVVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDAM
TADWTRLPYDVLEKISTRITNEVSDVNRVVLDCTSKPPGTIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=551, Percent_Identity=35.5716878402904, Blast_Score=276, Evalue=3e-74,
Organism=Escherichia coli, GI1788854, Length=536, Percent_Identity=47.3880597014925, Blast_Score=463, Evalue=1e-131,
Organism=Escherichia coli, GI1787517, Length=214, Percent_Identity=26.6355140186916, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71992710, Length=577, Percent_Identity=35.3552859618718, Blast_Score=275, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI71992717, Length=577, Percent_Identity=35.3552859618718, Blast_Score=275, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI133901714, Length=577, Percent_Identity=35.3552859618718, Blast_Score=274, Evalue=8e-74,
Organism=Saccharomyces cerevisiae, GI6323873, Length=526, Percent_Identity=45.4372623574144, Blast_Score=462, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322638, Length=178, Percent_Identity=30.3370786516854, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281365319, Length=560, Percent_Identity=34.4642857142857, Blast_Score=266, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_CORDI (P60499)

Other databases:

- EMBL:   BX248355
- RefSeq:   NP_938969.1
- ProteinModelPortal:   P60499
- SMR:   P60499
- GeneID:   2649349
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0595
- NMPDR:   fig|257309.1.peg.560
- HOGENOM:   HBG391775
- OMA:   PSTIEPC
- ProtClustDB:   PRK00074
- BioCyc:   CDIP257309:DIP0595-MONOMER
- BRENDA:   6.3.5.2
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase

EC number: =6.3.5.2

Molecular weight: Translated: 56255; Mature: 56124

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 88-88 ACT_SITE 174-174 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQTSQKHNPVLVVDFGAQYAQLIARRVREANIYSEVVPHTATVEEIKAKNPAALILSG
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEC
GPSSVYADGAPSLDPQVLELGIPVFGICYGFQAMTHALGGTVANTGNREYGRTEMTVDGG
CCCCEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEECCC
ILHADLEETHKVWMSHGDAVSVAPEGFVVTAHSEGAPVAAFECAEKRMAGVQYHPEVLHS
EEECCHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHCC
PHGQEVMVRFLTEIAGLEQNWTAANIAEELIDAVCAQVGPEGRAICGLSGGVDSAVAAAL
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHH
VQRAIGDRLTCVFVDHGLLRAGEREQVQTDFVAATGAKLVTVDEREAFLNKLAGVTEPEA
HHHHHCCCEEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCHH
KRKAIGAEFIRSFERAVAGILDDAPEGSTVDFLVQGTLYPDVVESGGGSGTANIKSHHNV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCC
GGLPDDVEFQLVEPLRLLFKDEVRAVGRELGLPEEIVNRQPFPGPGLGIRIIGEVTEERL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCEEEHHHHHHHHH
ETLRAADLIARTELTAAGLDSEIWQCPVVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
MTADWTRLPYDVLEKISTRITNEVSDVNRVVLDCTSKPPGTIEWE
CCCCHHCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
TQQTSQKHNPVLVVDFGAQYAQLIARRVREANIYSEVVPHTATVEEIKAKNPAALILSG
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEC
GPSSVYADGAPSLDPQVLELGIPVFGICYGFQAMTHALGGTVANTGNREYGRTEMTVDGG
CCCCEECCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEECCC
ILHADLEETHKVWMSHGDAVSVAPEGFVVTAHSEGAPVAAFECAEKRMAGVQYHPEVLHS
EEECCHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHCC
PHGQEVMVRFLTEIAGLEQNWTAANIAEELIDAVCAQVGPEGRAICGLSGGVDSAVAAAL
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHH
VQRAIGDRLTCVFVDHGLLRAGEREQVQTDFVAATGAKLVTVDEREAFLNKLAGVTEPEA
HHHHHCCCEEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCHH
KRKAIGAEFIRSFERAVAGILDDAPEGSTVDFLVQGTLYPDVVESGGGSGTANIKSHHNV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCC
GGLPDDVEFQLVEPLRLLFKDEVRAVGRELGLPEEIVNRQPFPGPGLGIRIIGEVTEERL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCEEEHHHHHHHHH
ETLRAADLIARTELTAAGLDSEIWQCPVVLLADVRSVGVQGDGRTYGHPIVLRPVSSEDA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCC
MTADWTRLPYDVLEKISTRITNEVSDVNRVVLDCTSKPPGTIEWE
CCCCHHCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910