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The map label for this gene is truA [H]

Identifier: 38233160

GI number: 38233160

Start: 519770

End: 520657

Strand: Direct

Name: truA [H]

Synonym: DIP0551

Alternate gene names: 38233160

Gene position: 519770-520657 (Clockwise)

Preceding gene: 38233159

Following gene: 38233161

Centisome position: 20.89

GC content: 58.22

Gene sequence:

>888_bases
TTGCTGCTAGCATTAATCGCTATGATTAACGGCACGGTACGCATCCGCCTCGATCTCGCATACGACGGCACCGACTTCCA
CGGCTGGGCGCGACAAGGCGACTCAGATCTACGCACCGTACAAAAAATAATCGAAGACACCCTTACACTCGTCCTCCAAC
GCCCCATCGACCTCACCGTCGCAGGACGCACCGACGCAGGCGTACACGCGACCGGACAAGTAGCACACTTCGACGTCGAC
CCAGCAGCCCTAGAAACCCGCAGTATTGCAGGCGACCCCGCTAGGTTGATACGTCGATTAGCACGATTGATGCCAGACGA
TGTCCGTATTCACAATATGAGCTTCGTACCAGCAGAATTCGACGCCCGATTCTCAGCACTACGCCGGCATTACGTCTACC
GCGTCACCACCCACCCCAGAGGCGCACTACCCACCCGCGCACGCGACACCGCCCACTGGCCACGCAGCGTCGATATTTCT
GCAATGCAGGCCGCCGCCGACGCATTGATCGGCATGCATGACTTTGCAGCGTTTTGCAAGTATCGCGAAGGCGCTAGCAC
CATCCGCGACCTACAAGAATTCACCTGGCATGACGTTTCAACTCCGCAAGAGCCCCAGCTCTACGAAGCCCACGTCACCG
CCGATGCCTTCTGCTGGTCGATGGTTCGCTCCCTTGTCGGAGCATGCCTAGTCGTGGGGGAGGGCAAGCGTGCTGATGGT
TTCACCGAACACCTTCTCGGAGAAACCAAGCGCTCATCCTCAATCCCCGTAGCAGCGGCGTGCGGTCTCAGTTTGGTCGG
CGTAGATTACCCCAGCGACGATCAATTAGGTGCACGTGCTGAGCAAGCACGTGCCTATCGGACCCACGACGAATTGCCTT
CCCTATAA

Upstream 100 bases:

>100_bases
AAGTAAAATTCTCTTATGGAGCGCACCAAATGCGCCCCATAGCCTACAACCGCACCACACAACTCCCCCGCACGCGAGGG
ACACGTGGTGCGGTTTTTCG

Downstream 100 bases:

>100_bases
AAATTGCGTTATCTGGGAACCTTCCTAGGTGTTGATTACGTCTAAACACATGGGGGGAAGTTCACACATCGGCTATCTCC
TTGCTCATCGCAGTGCGACC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MLLALIAMINGTVRIRLDLAYDGTDFHGWARQGDSDLRTVQKIIEDTLTLVLQRPIDLTVAGRTDAGVHATGQVAHFDVD
PAALETRSIAGDPARLIRRLARLMPDDVRIHNMSFVPAEFDARFSALRRHYVYRVTTHPRGALPTRARDTAHWPRSVDIS
AMQAAADALIGMHDFAAFCKYREGASTIRDLQEFTWHDVSTPQEPQLYEAHVTADAFCWSMVRSLVGACLVVGEGKRADG
FTEHLLGETKRSSSIPVAAACGLSLVGVDYPSDDQLGARAEQARAYRTHDELPSL

Sequences:

>Translated_295_residues
MLLALIAMINGTVRIRLDLAYDGTDFHGWARQGDSDLRTVQKIIEDTLTLVLQRPIDLTVAGRTDAGVHATGQVAHFDVD
PAALETRSIAGDPARLIRRLARLMPDDVRIHNMSFVPAEFDARFSALRRHYVYRVTTHPRGALPTRARDTAHWPRSVDIS
AMQAAADALIGMHDFAAFCKYREGASTIRDLQEFTWHDVSTPQEPQLYEAHVTADAFCWSMVRSLVGACLVVGEGKRADG
FTEHLLGETKRSSSIPVAAACGLSLVGVDYPSDDQLGARAEQARAYRTHDELPSL
>Mature_295_residues
MLLALIAMINGTVRIRLDLAYDGTDFHGWARQGDSDLRTVQKIIEDTLTLVLQRPIDLTVAGRTDAGVHATGQVAHFDVD
PAALETRSIAGDPARLIRRLARLMPDDVRIHNMSFVPAEFDARFSALRRHYVYRVTTHPRGALPTRARDTAHWPRSVDIS
AMQAAADALIGMHDFAAFCKYREGASTIRDLQEFTWHDVSTPQEPQLYEAHVTADAFCWSMVRSLVGACLVVGEGKRADG
FTEHLLGETKRSSSIPVAAACGLSLVGVDYPSDDQLGARAEQARAYRTHDELPSL

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=289, Percent_Identity=31.4878892733564, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI199559785, Length=284, Percent_Identity=27.8169014084507, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1788657, Length=258, Percent_Identity=35.2713178294574, Blast_Score=132, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321112, Length=234, Percent_Identity=28.6324786324786, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI116007752, Length=309, Percent_Identity=24.9190938511327, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 32546; Mature: 32546

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLALIAMINGTVRIRLDLAYDGTDFHGWARQGDSDLRTVQKIIEDTLTLVLQRPIDLTV
CHHHHEEHHCCEEEEEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
AGRTDAGVHATGQVAHFDVDPAALETRSIAGDPARLIRRLARLMPDDVRIHNMSFVPAEF
ECCCCCCCCCCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEEECCCHH
DARFSALRRHYVYRVTTHPRGALPTRARDTAHWPRSVDISAMQAAADALIGMHDFAAFCK
HHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YREGASTIRDLQEFTWHDVSTPQEPQLYEAHVTADAFCWSMVRSLVGACLVVGEGKRADG
HHCCHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
FTEHLLGETKRSSSIPVAAACGLSLVGVDYPSDDQLGARAEQARAYRTHDELPSL
HHHHHHCCCCCCCCCCCHHHCCEEEEECCCCCCCCCCCHHHHHHHHHCHHCCCCC
>Mature Secondary Structure
MLLALIAMINGTVRIRLDLAYDGTDFHGWARQGDSDLRTVQKIIEDTLTLVLQRPIDLTV
CHHHHEEHHCCEEEEEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
AGRTDAGVHATGQVAHFDVDPAALETRSIAGDPARLIRRLARLMPDDVRIHNMSFVPAEF
ECCCCCCCCCCCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEEEECCCHH
DARFSALRRHYVYRVTTHPRGALPTRARDTAHWPRSVDISAMQAAADALIGMHDFAAFCK
HHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
YREGASTIRDLQEFTWHDVSTPQEPQLYEAHVTADAFCWSMVRSLVGACLVVGEGKRADG
HHCCHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHEEEECCCCCCCC
FTEHLLGETKRSSSIPVAAACGLSLVGVDYPSDDQLGARAEQARAYRTHDELPSL
HHHHHHCCCCCCCCCCCHHHCCEEEEECCCCCCCCCCCHHHHHHHHHCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910 [H]