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The map label for this gene is narH [H]

Identifier: 38233111

GI number: 38233111

Start: 465007

End: 466608

Strand: Reverse

Name: narH [H]

Synonym: DIP0499

Alternate gene names: 38233111

Gene position: 466608-465007 (Counterclockwise)

Preceding gene: 38233112

Following gene: 38233110

Centisome position: 18.75

GC content: 52.87

Gene sequence:

>1602_bases
ATGAAGGTCATGGCTCAGATCGCAATGATCATGAACCTTGATAAATGTATTGGTTGCCACACTTGTTCTGTGACGTGCAA
GCAGGCATGGACTAACCGTGAAGGCACCGAATACATTTGGTTCAATAACGTCGAGACGCGCCCCGGCGTCGGCTACCCCT
ATGGTTGGGAAAACCAAGACAAATGGGAAGGTGGCTGGGTATTGGACAAGAAAGGCAAGCTCAAGCCACGTTCCGGTGGT
CGCCTGAAAAAGCTCAGCACACTATTCCATAACCCTAAGCTGCCTACGATTCAGGATTACTACGAGCCGTGGACCTATGA
GTATGAAAAGCTGCTGTCTACTCCAGCAGGGCAAAAAACTCAGCCCACTGCTCGCCCGATTTCACAGGTCGACGGCCGCC
CCATCGATAAGATCTCATGGTCTTCTAACTGGGACGATAACTTGGGTGGCTCCCAAGAGACTCTCGACGATGATCCTGTG
TTGAAGCAGATGAATCTTGAGGTCAAAAAGGAGATCGAAGATGCCTTCATGTTCTATCTCCCACGCATCTGCGAGCACTG
CCTCAACCCTACGTGTGTGTCATCGTGCCCTTCAGGTGCGATGTATAAACGTGCTGAAGACGGCATTGTCCTTGTTGACC
AAGATCGTTGCCGTGGTTGGCGCATGTGTGTTTCCGGCTGCCCGTACAAGAAGGTCTACTTCAACCACAAGACTGGTAAA
GCTGAGAAATGTACGCTTTGCTACCCACGCATCGAGGTCGGTCAGCCGACTGTTTGCTCCGAGACATGCGTTGGCCGTAT
GCGTTATCTCGGTGTTGTGCTTTACGACGCTGACAAAGTAGCCAGTGCCGCACGTACCTCAGATGTTAAGGATCTCTATC
AGGCTCAGAAAAATCTGCTGCTTGATCCGCACGATCCACAGGTGATCGAGGCTGCGATGAAGGAAAATATTCCTCATGCG
TGGATCGAGGCGGCACAAAAGTCTCCCATCTGGGATTTGATCGCACGCTACGAAGTCGCGCTCCCCCTGCACCCTGAGTA
CCGCACACTACCGATGGTGTGGTATATCCCGCCGCTAAGCCCAGTAGTTGATCGCGTCACCGCCACCGGCAACGATGGCG
AGGACCATAAGATTTTGCTCACAGCGATCTCTACTATGCGTATCCCTCTGGATTATCTAGCCGGCATTTTTACCGCTGGG
GATACCGTTCCCGTGGAAAAGGTGCTGCGTAAACTTGCGGCGATGCGCTCGTATATGCGTGATATTTCTCTGGGCAAAAC
AGCAGATGAGTCGATTCCCGCTGCTGTGGGCATGACGGGCGCTGACATTCGTAACATGTACCACCTGCTCGGTGTGTCCA
AATACGATGACCGCTACGTTATTCCTACGGCTTCTCCAGAAACACCACGTGGTATTGCTTCCTTGCCGGAATATGCTGGT
ATCAATCCGGCACAGTCTGTTGAACAGTTCCACGGCCTAGGTCCCGGCGCGCCGGAGGCTTGCCACCATGAGGCTCAAAG
TTCTGGTGGCGGTAAGGTGTCGTTGGCTTCGTGGAATGTGGGAAGTAGCCCAAGTAGCATGTTCCCGCCGAGAAAGAGCT
AG

Upstream 100 bases:

>100_bases
ACGGCCAGCTGACCTACCATTTCAATTACATTGGTCCGACCGGTAACAACCGCGACGAAGTCACACGTATTCGTCGCCGT
TCCCAGGAGGTGCAGTACTA

Downstream 100 bases:

>100_bases
ATCATGCGTACTCATACGGGGGCCGTTCCGGCCCCTACCTCTCGGGTGGCGATGACTGATCAGCAGCGCAGGGTTGTCTT
TATGGCGACGTCGTTGTTGT

Product: nitrate reductase beta chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKVMAQIAMIMNLDKCIGCHTCSVTCKQAWTNREGTEYIWFNNVETRPGVGYPYGWENQDKWEGGWVLDKKGKLKPRSGG
RLKKLSTLFHNPKLPTIQDYYEPWTYEYEKLLSTPAGQKTQPTARPISQVDGRPIDKISWSSNWDDNLGGSQETLDDDPV
LKQMNLEVKKEIEDAFMFYLPRICEHCLNPTCVSSCPSGAMYKRAEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGK
AEKCTLCYPRIEVGQPTVCSETCVGRMRYLGVVLYDADKVASAARTSDVKDLYQAQKNLLLDPHDPQVIEAAMKENIPHA
WIEAAQKSPIWDLIARYEVALPLHPEYRTLPMVWYIPPLSPVVDRVTATGNDGEDHKILLTAISTMRIPLDYLAGIFTAG
DTVPVEKVLRKLAAMRSYMRDISLGKTADESIPAAVGMTGADIRNMYHLLGVSKYDDRYVIPTASPETPRGIASLPEYAG
INPAQSVEQFHGLGPGAPEACHHEAQSSGGGKVSLASWNVGSSPSSMFPPRKS

Sequences:

>Translated_533_residues
MKVMAQIAMIMNLDKCIGCHTCSVTCKQAWTNREGTEYIWFNNVETRPGVGYPYGWENQDKWEGGWVLDKKGKLKPRSGG
RLKKLSTLFHNPKLPTIQDYYEPWTYEYEKLLSTPAGQKTQPTARPISQVDGRPIDKISWSSNWDDNLGGSQETLDDDPV
LKQMNLEVKKEIEDAFMFYLPRICEHCLNPTCVSSCPSGAMYKRAEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGK
AEKCTLCYPRIEVGQPTVCSETCVGRMRYLGVVLYDADKVASAARTSDVKDLYQAQKNLLLDPHDPQVIEAAMKENIPHA
WIEAAQKSPIWDLIARYEVALPLHPEYRTLPMVWYIPPLSPVVDRVTATGNDGEDHKILLTAISTMRIPLDYLAGIFTAG
DTVPVEKVLRKLAAMRSYMRDISLGKTADESIPAAVGMTGADIRNMYHLLGVSKYDDRYVIPTASPETPRGIASLPEYAG
INPAQSVEQFHGLGPGAPEACHHEAQSSGGGKVSLASWNVGSSPSSMFPPRKS
>Mature_533_residues
MKVMAQIAMIMNLDKCIGCHTCSVTCKQAWTNREGTEYIWFNNVETRPGVGYPYGWENQDKWEGGWVLDKKGKLKPRSGG
RLKKLSTLFHNPKLPTIQDYYEPWTYEYEKLLSTPAGQKTQPTARPISQVDGRPIDKISWSSNWDDNLGGSQETLDDDPV
LKQMNLEVKKEIEDAFMFYLPRICEHCLNPTCVSSCPSGAMYKRAEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGK
AEKCTLCYPRIEVGQPTVCSETCVGRMRYLGVVLYDADKVASAARTSDVKDLYQAQKNLLLDPHDPQVIEAAMKENIPHA
WIEAAQKSPIWDLIARYEVALPLHPEYRTLPMVWYIPPLSPVVDRVTATGNDGEDHKILLTAISTMRIPLDYLAGIFTAG
DTVPVEKVLRKLAAMRSYMRDISLGKTADESIPAAVGMTGADIRNMYHLLGVSKYDDRYVIPTASPETPRGIASLPEYAG
INPAQSVEQFHGLGPGAPEACHHEAQSSGGGKVSLASWNVGSSPSSMFPPRKS

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E. coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Beta Chain Is An Electron Transfer Unit Containing Four Cysteine Clusters Involved In

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=467, Percent_Identity=55.8886509635974, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1787478, Length=482, Percent_Identity=54.149377593361, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1787872, Length=132, Percent_Identity=35.6060606060606, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=35.6060606060606, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789370, Length=102, Percent_Identity=38.2352941176471, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787749, Length=80, Percent_Identity=37.5, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI2367345, Length=104, Percent_Identity=32.6923076923077, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1790326, Length=107, Percent_Identity=30.8411214953271, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=37.2093023255814, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 59435; Mature: 59435

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVMAQIAMIMNLDKCIGCHTCSVTCKQAWTNREGTEYIWFNNVETRPGVGYPYGWENQD
CHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWEGGWVLDKKGKLKPRSGGRLKKLSTLFHNPKLPTIQDYYEPWTYEYEKLLSTPAGQKT
CCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCC
QPTARPISQVDGRPIDKISWSSNWDDNLGGSQETLDDDPVLKQMNLEVKKEIEDAFMFYL
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHH
PRICEHCLNPTCVSSCPSGAMYKRAEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGK
HHHHHHHCCCHHHHCCCCCCHHHCCCCCEEEEECHHCCHHHHHHCCCCCEEEEEECCCCC
AEKCTLCYPRIEVGQPTVCSETCVGRMRYLGVVLYDADKVASAARTSDVKDLYQAQKNLL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
LDPHDPQVIEAAMKENIPHAWIEAAQKSPIWDLIARYEVALPLHPEYRTLPMVWYIPPLS
CCCCCHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCEEEEECCCC
PVVDRVTATGNDGEDHKILLTAISTMRIPLDYLAGIFTAGDTVPVEKVLRKLAAMRSYMR
HHHHHHCCCCCCCCCCEEEEEEHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
DISLGKTADESIPAAVGMTGADIRNMYHLLGVSKYDDRYVIPTASPETPRGIASLPEYAG
HHCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHCCCHHCC
INPAQSVEQFHGLGPGAPEACHHEAQSSGGGKVSLASWNVGSSPSSMFPPRKS
CCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVMAQIAMIMNLDKCIGCHTCSVTCKQAWTNREGTEYIWFNNVETRPGVGYPYGWENQD
CHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWEGGWVLDKKGKLKPRSGGRLKKLSTLFHNPKLPTIQDYYEPWTYEYEKLLSTPAGQKT
CCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHCCCCCCCC
QPTARPISQVDGRPIDKISWSSNWDDNLGGSQETLDDDPVLKQMNLEVKKEIEDAFMFYL
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHHHHHHH
PRICEHCLNPTCVSSCPSGAMYKRAEDGIVLVDQDRCRGWRMCVSGCPYKKVYFNHKTGK
HHHHHHHCCCHHHHCCCCCCHHHCCCCCEEEEECHHCCHHHHHHCCCCCEEEEEECCCCC
AEKCTLCYPRIEVGQPTVCSETCVGRMRYLGVVLYDADKVASAARTSDVKDLYQAQKNLL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
LDPHDPQVIEAAMKENIPHAWIEAAQKSPIWDLIARYEVALPLHPEYRTLPMVWYIPPLS
CCCCCHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHEEECCCCCCCCCCEEEEECCCC
PVVDRVTATGNDGEDHKILLTAISTMRIPLDYLAGIFTAGDTVPVEKVLRKLAAMRSYMR
HHHHHHCCCCCCCCCCEEEEEEHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
DISLGKTADESIPAAVGMTGADIRNMYHLLGVSKYDDRYVIPTASPETPRGIASLPEYAG
HHCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHCCCHHCC
INPAQSVEQFHGLGPGAPEACHHEAQSSGGGKVSLASWNVGSSPSSMFPPRKS
CCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 7557333; 9384377 [H]