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The map label for this gene is tuf [H]

Identifier: 38233082

GI number: 38233082

Start: 440423

End: 441613

Strand: Direct

Name: tuf [H]

Synonym: DIP0470

Alternate gene names: 38233082

Gene position: 440423-441613 (Clockwise)

Preceding gene: 38233081

Following gene: 38233083

Centisome position: 17.7

GC content: 57.6

Gene sequence:

>1191_bases
GTGGCAAAGGCTAAGTTCGAGCGTACCAAGCCGCACGTCAACATCGGCACCATCGGTCACGTTGACCACGGTAAGACCAC
CACCACCGCTGCTATCACCAAGGTTTTGGCAGACGCTTACCCAGAGCTGAACGAAGCTTTCGCTTTCGATGCCATCGATA
AGGCACCGGAAGAGAAAGAGCGTGGTATTACCATCAACATCTCCCACGTGGAGTACCAGACCGAGAAGCGCCACTACGCA
CACGTTGACGCTCCAGGTCACGCTGACTACATCAAGAACATGATCACCGGTGCTGCTCAGATGGACGGCGCAATCCTCGT
TGTTGCTGCCACCGACGGCCCAATGCCTCAGACCCGTGAGCACGTTCTGCTCGCTCGCCAGGTCGGCGTTCCTTACATCC
TCGTTGCTCTGAACAAGTGCGACATGGTTGATGATGAGGAAATCATCGAGCTCGTCGAGATGGAGATCCGTGAGCTGCTC
GCTGAGCAGGATTACGACGAAGAGGCTCCAATCATCCACATCTCCGCACTGAAGGCTCTTGAGGGCGACGAGAAGTGGAC
CCAGTCCATCATCGACCTCATGCAGGCTTGCGATGATTCCATCCCAGACCCAGAGCGTGAGACCGACAAGCCATTCCTCA
TGCCTATCGAGGACATCTTCACCATCACCGGCCGCGGTACCGTTGTTACCGGCCGTGTTGAGCGTGGCTCCCTGAAGGTC
AACGAGGACGTCGAGATCATCGGTATCCGCGAGAAGGCTACCACCACCACCGTTACCGGTATCGAGATGTTCCGTAAGCT
TCTCGACTACACCGAGGCTGGCGACAACTGTGGTCTGCTTCTCCGTGGCGTTAAGCGCGAAGACGTTGAGCGTGGCCAGG
TTGTTGTTAAGCCAGGCGCTTACACCCCTCACACCGAGTTCGAGGGCTCTGTCTACGTTCTGTCCAAGGACGAGGGTGGC
CGCCACACCCCATTCTTCGACAACTACCGCCCACAGTTCTACTTCCGCACCACCGACGTTACCGGTGTTGTGAAGCTTCC
TGAGGGCACCGAGATGGTCATGCCTGGCGACAACGTCGACATGTCCGTCACCCTGATCCAGCCTGTCGCTATGGATGAGG
GCCTGCGCTTCGCTATCCGCGAGGGCTCCCGCACCGTCGGCGCTGGTCGCGTTACCAAGATCATCAAGTAA

Upstream 100 bases:

>100_bases
GCTGTACTGCCAGGCTGCCGAATTAGCAGTCAGAAATGTACAGCACTGTCAACTCGTGGCTGCGAAATCGTAGCCACCAC
GAAGTCCAGGAGGACACACA

Downstream 100 bases:

>100_bases
TGATCAACTGCGCCTAGGCGCATAGAATTGCCGCTTCCCCACAAAGGGGAGGCGGCTTTTCTCGTATTATCGAACCTATG
CTAAATCCCCACTTCCACGA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTTTAAITKVLADAYPELNEAFAFDAIDKAPEEKERGITINISHVEYQTEKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKCDMVDDEEIIELVEMEIRELL
AEQDYDEEAPIIHISALKALEGDEKWTQSIIDLMQACDDSIPDPERETDKPFLMPIEDIFTITGRGTVVTGRVERGSLKV
NEDVEIIGIREKATTTTVTGIEMFRKLLDYTEAGDNCGLLLRGVKREDVERGQVVVKPGAYTPHTEFEGSVYVLSKDEGG
RHTPFFDNYRPQFYFRTTDVTGVVKLPEGTEMVMPGDNVDMSVTLIQPVAMDEGLRFAIREGSRTVGAGRVTKIIK

Sequences:

>Translated_396_residues
MAKAKFERTKPHVNIGTIGHVDHGKTTTTAAITKVLADAYPELNEAFAFDAIDKAPEEKERGITINISHVEYQTEKRHYA
HVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKCDMVDDEEIIELVEMEIRELL
AEQDYDEEAPIIHISALKALEGDEKWTQSIIDLMQACDDSIPDPERETDKPFLMPIEDIFTITGRGTVVTGRVERGSLKV
NEDVEIIGIREKATTTTVTGIEMFRKLLDYTEAGDNCGLLLRGVKREDVERGQVVVKPGAYTPHTEFEGSVYVLSKDEGG
RHTPFFDNYRPQFYFRTTDVTGVVKLPEGTEMVMPGDNVDMSVTLIQPVAMDEGLRFAIREGSRTVGAGRVTKIIK
>Mature_395_residues
AKAKFERTKPHVNIGTIGHVDHGKTTTTAAITKVLADAYPELNEAFAFDAIDKAPEEKERGITINISHVEYQTEKRHYAH
VDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKCDMVDDEEIIELVEMEIRELLA
EQDYDEEAPIIHISALKALEGDEKWTQSIIDLMQACDDSIPDPERETDKPFLMPIEDIFTITGRGTVVTGRVERGSLKVN
EDVEIIGIREKATTTTVTGIEMFRKLLDYTEAGDNCGLLLRGVKREDVERGQVVVKPGAYTPHTEFEGSVYVLSKDEGGR
HTPFFDNYRPQFYFRTTDVTGVVKLPEGTEMVMPGDNVDMSVTLIQPVAMDEGLRFAIREGSRTVGAGRVTKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=398, Percent_Identity=55.0251256281407, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI4503471, Length=455, Percent_Identity=31.2087912087912, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4503475, Length=445, Percent_Identity=29.6629213483146, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI223555963, Length=445, Percent_Identity=28.314606741573, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI5729864, Length=445, Percent_Identity=28.314606741573, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI46094014, Length=332, Percent_Identity=29.5180722891566, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194018522, Length=327, Percent_Identity=29.3577981651376, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194097354, Length=331, Percent_Identity=28.7009063444109, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI194018520, Length=331, Percent_Identity=28.7009063444109, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI54607086, Length=325, Percent_Identity=27.6923076923077, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4503507, Length=375, Percent_Identity=28.2666666666667, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI53729339, Length=233, Percent_Identity=31.3304721030043, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI53729337, Length=233, Percent_Identity=31.3304721030043, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=72.4747474747475, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=72.4747474747475, Blast_Score=566, Evalue=1e-163,
Organism=Escherichia coli, GI2367247, Length=384, Percent_Identity=29.6875, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI48994988, Length=273, Percent_Identity=33.6996336996337, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789108, Length=262, Percent_Identity=27.0992366412214, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788922, Length=329, Percent_Identity=26.1398176291793, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=48.7562189054726, Blast_Score=357, Evalue=7e-99,
Organism=Caenorhabditis elegans, GI25141371, Length=376, Percent_Identity=44.6808510638298, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17552884, Length=446, Percent_Identity=29.8206278026906, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=446, Percent_Identity=29.8206278026906, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI115532067, Length=326, Percent_Identity=30.9815950920245, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=326, Percent_Identity=30.9815950920245, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI32566303, Length=318, Percent_Identity=32.0754716981132, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=274, Percent_Identity=31.3868613138686, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=335, Percent_Identity=25.6716417910448, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=148, Percent_Identity=43.2432432432432, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=232, Percent_Identity=28.448275862069, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17509919, Length=305, Percent_Identity=26.5573770491803, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=400, Percent_Identity=63, Blast_Score=493, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6325337, Length=443, Percent_Identity=30.2483069977427, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319594, Length=443, Percent_Identity=30.2483069977427, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6322937, Length=338, Percent_Identity=26.6272189349112, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=330, Percent_Identity=26.6666666666667, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=246, Percent_Identity=29.2682926829268, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320863, Length=289, Percent_Identity=26.2975778546713, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=56.3131313131313, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=56.3131313131313, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=49.1002570694087, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45553807, Length=447, Percent_Identity=30.2013422818792, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45553816, Length=447, Percent_Identity=30.2013422818792, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24651721, Length=447, Percent_Identity=30.2013422818792, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17864154, Length=447, Percent_Identity=30.2013422818792, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.2410714285714, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.2410714285714, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=29.8360655737705, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI45550900, Length=340, Percent_Identity=29.1176470588235, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI17137380, Length=317, Percent_Identity=28.391167192429, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24647046, Length=372, Percent_Identity=27.4193548387097, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24647048, Length=372, Percent_Identity=27.4193548387097, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI78706572, Length=315, Percent_Identity=26.031746031746, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28572034, Length=166, Percent_Identity=37.3493975903614, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43945; Mature: 43813

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHVNIGTIGHVDHGKTTTTAAITKVLADAYPELNEAFAFDAIDKAPEEKE
CCCCCCCCCCCCEEECEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHH
RGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKCDMVDDEEIIELVEMEIRELLAEQDYDEEAPIIHISALKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHH
EGDEKWTQSIIDLMQACDDSIPDPERETDKPFLMPIEDIFTITGRGTVVTGRVERGSLKV
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHEEEECCCEEEEEEEECCCEEE
NEDVEIIGIREKATTTTVTGIEMFRKLLDYTEAGDNCGLLLRGVKREDVERGQVVVKPGA
CCCEEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCC
YTPHTEFEGSVYVLSKDEGGRHTPFFDNYRPQFYFRTTDVTGVVKLPEGTEMVMPGDNVD
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCC
MSVTLIQPVAMDEGLRFAIREGSRTVGAGRVTKIIK
EEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHVNIGTIGHVDHGKTTTTAAITKVLADAYPELNEAFAFDAIDKAPEEKE
CCCCCCCCCCCEEECEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHH
RGITINISHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPYILVALNKCDMVDDEEIIELVEMEIRELLAEQDYDEEAPIIHISALKAL
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHH
EGDEKWTQSIIDLMQACDDSIPDPERETDKPFLMPIEDIFTITGRGTVVTGRVERGSLKV
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHEEEECCCEEEEEEEECCCEEE
NEDVEIIGIREKATTTTVTGIEMFRKLLDYTEAGDNCGLLLRGVKREDVERGQVVVKPGA
CCCEEEEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEECCCC
YTPHTEFEGSVYVLSKDEGGRHTPFFDNYRPQFYFRTTDVTGVVKLPEGTEMVMPGDNVD
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCC
MSVTLIQPVAMDEGLRFAIREGSRTVGAGRVTKIIK
EEEEEEEEEECCCCCEEEEECCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]