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The map label for this gene is menD

Identifier: 38233037

GI number: 38233037

Start: 387917

End: 389446

Strand: Direct

Name: menD

Synonym: DIP0423

Alternate gene names: 38233037

Gene position: 387917-389446 (Clockwise)

Preceding gene: 38233036

Following gene: 38233038

Centisome position: 15.59

GC content: 54.38

Gene sequence:

>1530_bases
ATGACTTCATCCCCTGAGCTTGCCCGCGCAGTCGCAGCGACCCTTGCCCGCCACGTCACTGACGTTGTTATTTGTCCTGG
GTCGCGGAATTCTCCGCTGTCTTTGGAACTAATTGCTCGCGATGATATTCGTGTGCACACGCGTATCGACGAACGCTCGG
CGGCGTTTTTGGCTCTCGGACTTGCACGTTCTAGCAAGCGTCACGTTGCTGTGGTGACTACTTCTGGTACCGCTGTGGCT
AACTGTGCTCCTGCGATGATTGAGGCGGCGTATTCTCATACTCCGTTGATTATGGTGTCGGCGGATCGTCCTGAGCGTCT
GCATGGTACGGGGGCAAATCAGACGATTGAGCAGCGTGGCATTTTTAATGTGGTGGATACGGATCATGTGGTTGATATTA
CTGACCTTAACTCCATTTCCTTTGCGCGCAATGTAATCCATATCAATGTTGCATTGGATGTGCCACTTGTGGATGTGTTG
CCTGAAGTAGGTGATCCGGTGGAACGCGTTCGTGAAGTGATTGAGGTTGATCATGGCGAAGTTGTACTCAATCTAAATGA
AAGAATTCTCGTGGTAGCTGGCGATGAGGCGTGGGAAGTTCCTGGATTGGAGGATGTTCCTACGATTGCAGAACCATCGG
CTCCGGCGCCGTATCATCCGGTACATCCGCTGGCGGCTCAAATCTTTGCGCACAAGGAAATTAAACCCGAACATATTGTG
GTGGTGGGGCACCCTACGCTGCACCGTGCGGTGCTGTCATTGTTGGCCGATGTTCCGGTAACGGTGCTGACTAAGACCGA
TACAATCACTGGCAATCCCCAAGCGGTAGGCAGTCGCGTGAAGGTGACGGGGGAGCTAGATAAGCGTTGGTTGCAGATCT
GTGAGGATGCATCGACGCTGTGTGCGGATCGGGTAAAGGAAGTGCTGGAGGGGCATGGTTTTACTGGCCTGCATGTTGCG
GCTGCGGTTGCAGACACGATGGCTGTGGGCGACACACTATTTTTGGGTGCGTCAAATCCGGTTCGCGATGCCTCCTTGGT
GGGAATGCCACTGGGTGGAGTGGATATTTTCGCAGCTCGTGGGGCTGCGGGTATCGACGGCACCGTGTCGCAGGCGGTCG
GCGTGGCGCTGGATGTGCAGCACCGTGACGCGACGGCAATTCGTGCTGATCGCACGGTGGCGCTGATGGGTGATGTGACA
TTCTTGCATGATGTTGGTGGCTTGTTGGGGGCACGTCCAGAGAATCTGACTATCGTGGTGGCAAACGATGATGGCTGCGG
CATTTTTGAAACACTTGAGATTGGGGCTCCAGAGTTCCGCCCCAGTTTTGAACAGGCCTTTGGTACTCCGCATGGTGTGC
ACATTGAGGCAATTGCGCAGGCCTATGGGGTGAACTACCTGCGTGCGGAAACACTGCCGGAGTTGATTGAGGCGTTGATT
GATACGACGGATAGTGCCGGATTCAATATTATTGAGGCTGTGACTACGCGTTCGACGCGTCGCGATATTGATAAGGCTCT
CACGCTGTGA

Upstream 100 bases:

>100_bases
ATGCTGGCTCCTGATCCGGCGCGCTTGGCGGAATTCGAGGTCACTGGCCCGCGCCGGGATTGGTGGCTGGATCGAGTTCG
GGGCGCGTTAGAATGTCTGC

Downstream 100 bases:

>100_bases
AACGCCGCCTCCATCAACTTGTCCTAGCTTTGTATGCGGCAGCCGTCTTGGGATGCGCCAGCATGGTTATTGGTCCTGCG
ATCAATGACTATCACATTGC

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTSSPELARAVAATLARHVTDVVICPGSRNSPLSLELIARDDIRVHTRIDERSAAFLALGLARSSKRHVAVVTTSGTAVA
NCAPAMIEAAYSHTPLIMVSADRPERLHGTGANQTIEQRGIFNVVDTDHVVDITDLNSISFARNVIHINVALDVPLVDVL
PEVGDPVERVREVIEVDHGEVVLNLNERILVVAGDEAWEVPGLEDVPTIAEPSAPAPYHPVHPLAAQIFAHKEIKPEHIV
VVGHPTLHRAVLSLLADVPVTVLTKTDTITGNPQAVGSRVKVTGELDKRWLQICEDASTLCADRVKEVLEGHGFTGLHVA
AAVADTMAVGDTLFLGASNPVRDASLVGMPLGGVDIFAARGAAGIDGTVSQAVGVALDVQHRDATAIRADRTVALMGDVT
FLHDVGGLLGARPENLTIVVANDDGCGIFETLEIGAPEFRPSFEQAFGTPHGVHIEAIAQAYGVNYLRAETLPELIEALI
DTTDSAGFNIIEAVTTRSTRRDIDKALTL

Sequences:

>Translated_509_residues
MTSSPELARAVAATLARHVTDVVICPGSRNSPLSLELIARDDIRVHTRIDERSAAFLALGLARSSKRHVAVVTTSGTAVA
NCAPAMIEAAYSHTPLIMVSADRPERLHGTGANQTIEQRGIFNVVDTDHVVDITDLNSISFARNVIHINVALDVPLVDVL
PEVGDPVERVREVIEVDHGEVVLNLNERILVVAGDEAWEVPGLEDVPTIAEPSAPAPYHPVHPLAAQIFAHKEIKPEHIV
VVGHPTLHRAVLSLLADVPVTVLTKTDTITGNPQAVGSRVKVTGELDKRWLQICEDASTLCADRVKEVLEGHGFTGLHVA
AAVADTMAVGDTLFLGASNPVRDASLVGMPLGGVDIFAARGAAGIDGTVSQAVGVALDVQHRDATAIRADRTVALMGDVT
FLHDVGGLLGARPENLTIVVANDDGCGIFETLEIGAPEFRPSFEQAFGTPHGVHIEAIAQAYGVNYLRAETLPELIEALI
DTTDSAGFNIIEAVTTRSTRRDIDKALTL
>Mature_508_residues
TSSPELARAVAATLARHVTDVVICPGSRNSPLSLELIARDDIRVHTRIDERSAAFLALGLARSSKRHVAVVTTSGTAVAN
CAPAMIEAAYSHTPLIMVSADRPERLHGTGANQTIEQRGIFNVVDTDHVVDITDLNSISFARNVIHINVALDVPLVDVLP
EVGDPVERVREVIEVDHGEVVLNLNERILVVAGDEAWEVPGLEDVPTIAEPSAPAPYHPVHPLAAQIFAHKEIKPEHIVV
VGHPTLHRAVLSLLADVPVTVLTKTDTITGNPQAVGSRVKVTGELDKRWLQICEDASTLCADRVKEVLEGHGFTGLHVAA
AVADTMAVGDTLFLGASNPVRDASLVGMPLGGVDIFAARGAAGIDGTVSQAVGVALDVQHRDATAIRADRTVALMGDVTF
LHDVGGLLGARPENLTIVVANDDGCGIFETLEIGAPEFRPSFEQAFGTPHGVHIEAIAQAYGVNYLRAETLPELIEALID
TTDSAGFNIIEAVTTRSTRRDIDKALTL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=561, Percent_Identity=27.9857397504456, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_CORDI (Q6NJH8)

Other databases:

- EMBL:   BX248355
- RefSeq:   NP_938804.1
- ProteinModelPortal:   Q6NJH8
- GeneID:   2649096
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0423
- NMPDR:   fig|257309.1.peg.395
- HOGENOM:   HBG634405
- OMA:   IAEPTAP
- PhylomeDB:   Q6NJH8
- ProtClustDB:   PRK07449
- BioCyc:   CDIP257309:DIP0423-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 54052; Mature: 53921

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSPELARAVAATLARHVTDVVICPGSRNSPLSLELIARDDIRVHTRIDERSAAFLALG
CCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEECCCEEEEEEECCCCCEEEEEE
LARSSKRHVAVVTTSGTAVANCAPAMIEAAYSHTPLIMVSADRPERLHGTGANQTIEQRG
HHCCCCCEEEEEEECCCCHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHCC
IFNVVDTDHVVDITDLNSISFARNVIHINVALDVPLVDVLPEVGDPVERVREVIEVDHGE
CEEEECCCCEEEEECCCCHHEECEEEEEEEEECCCHHHHHHCCCCHHHHHHHHHCCCCCE
VVLNLNERILVVAGDEAWEVPGLEDVPTIAEPSAPAPYHPVHPLAAQIFAHKEIKPEHIV
EEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEE
VVGHPTLHRAVLSLLADVPVTVLTKTDTITGNPQAVGSRVKVTGELDKRWLQICEDASTL
EECCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHCCCEEEEECHHHHHHHHHHHHHHHH
CADRVKEVLEGHGFTGLHVAAAVADTMAVGDTLFLGASNPVRDASLVGMPLGGVDIFAAR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEECCCCCCEEEEEC
GAAGIDGTVSQAVGVALDVQHRDATAIRADRTVALMGDVTFLHDVGGLLGARPENLTIVV
CCCCCCCHHHHHCCEEEEECCCCCCEEECCCEEEEECCHHHHHHHHHHHCCCCCCEEEEE
ANDDGCGIFETLEIGAPEFRPSFEQAFGTPHGVHIEAIAQAYGVNYLRAETLPELIEALI
ECCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
DTTDSAGFNIIEAVTTRSTRRDIDKALTL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSSPELARAVAATLARHVTDVVICPGSRNSPLSLELIARDDIRVHTRIDERSAAFLALG
CCCHHHHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEECCCEEEEEEECCCCCEEEEEE
LARSSKRHVAVVTTSGTAVANCAPAMIEAAYSHTPLIMVSADRPERLHGTGANQTIEQRG
HHCCCCCEEEEEEECCCCHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHCC
IFNVVDTDHVVDITDLNSISFARNVIHINVALDVPLVDVLPEVGDPVERVREVIEVDHGE
CEEEECCCCEEEEECCCCHHEECEEEEEEEEECCCHHHHHHCCCCHHHHHHHHHCCCCCE
VVLNLNERILVVAGDEAWEVPGLEDVPTIAEPSAPAPYHPVHPLAAQIFAHKEIKPEHIV
EEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEE
VVGHPTLHRAVLSLLADVPVTVLTKTDTITGNPQAVGSRVKVTGELDKRWLQICEDASTL
EECCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHCCCEEEEECHHHHHHHHHHHHHHHH
CADRVKEVLEGHGFTGLHVAAAVADTMAVGDTLFLGASNPVRDASLVGMPLGGVDIFAAR
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEECCCCCCEEEEEC
GAAGIDGTVSQAVGVALDVQHRDATAIRADRTVALMGDVTFLHDVGGLLGARPENLTIVV
CCCCCCCHHHHHCCEEEEECCCCCCEEECCCEEEEECCHHHHHHHHHHHCCCCCCEEEEE
ANDDGCGIFETLEIGAPEFRPSFEQAFGTPHGVHIEAIAQAYGVNYLRAETLPELIEALI
ECCCCCCEEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
DTTDSAGFNIIEAVTTRSTRRDIDKALTL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910