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The map label for this gene is hemL

Identifier: 38233023

GI number: 38233023

Start: 376342

End: 377634

Strand: Direct

Name: hemL

Synonym: DIP0409

Alternate gene names: 38233023

Gene position: 376342-377634 (Clockwise)

Preceding gene: 38233022

Following gene: 38233024

Centisome position: 15.12

GC content: 56.84

Gene sequence:

>1293_bases
ATGACTTCTACGCAACGATCTCAGGAACTTTTCGCGCGCGCTCAGCAGTTCACTCCCGGTGGTGTGAATTCCCCTGTGCG
CGCTTTCGGCTCAGTGGGCGGCCACGCCCGTTTCATCCAGTCGGCGAAAGGGTCCAAGCTTTACGACGTAGACGGCAACG
AGTACGTCGATTTGGTCTGTTCGTGGGGCCCCATGTTGTTGGGTAATGCGCATCCGGAGATCGTTGAGGCTGTGCAGAAG
GCCGCAACCAACGGTTTGTCTTTCGGTGCTCCGACGGAGGCCGAGGTCAAGATCGCGGAGATGATTGTGGATCGTACTTC
CGTGGAGGAAGTCCGCATGGTCAATTCTGGTACTGAGGCGACGATGTCGGCGGTGCGGTTGGCTCGTGGCTTTACTGGCC
GTCCGAAGGTCATCAAGTTTGATGGCTGCTACCACGGTCATGTGGATGCGTTGTTAGCTTCTGCGGGTTCGGGCGTTGCG
ACGTTCGCGTTGCCGGATTCCCCTGGTGTGACGGGCGCGTCGGCTGCCGATACCATCGTGGTTCCTTATAACGATAGTGA
GGCAGTCCGTGCCGCTTTTGAGGCGCATCCTGATCAGATCGCTTGTGTGATTGCCGAGGCCGCCGCCGGAAACATGGGCA
CGGTGGCACCGCAGGATAACTTCAATGCCAAGCTCAAAGAGGTAGCGCATGAGCATGGTGCGCTGCTGATCTTGGATGAG
GTGATGACGGGCTTCCGTACCTCTTATAACGGTTGGTATGGCGTCGATGGTGTTGCAGGTGACCTCACCACGTTTGGCAA
GGTGATTTCCGGTGGTCTTCCTGCTGCTGCTTTTGGTGGTCGTGCGGACATTATGCGCTACCTTGCTCCTGAGGGTCCTG
TGTACCAAGCGGGTACGTTGTCGGGTAATCCGGTGGCGATGGCGGCTGGTATGAAGTCGTTGGAGCTTGCTACCCCTGAG
GTGTATGACATGGTGCGCGCTAATGCGGACCGCTTGGCTGGGTTGTTGCATGAGGCGTTGGATCGTGAGGGTGTTGCGCA
CCATATTCAGCGTGCTGCCACGATGTTGTCGGTTCGTTTCGCTGAGGGCGAGGGCCACAACTTTGATGATATGAAGGCTG
CGGATACGTTCCGGTTCCCTGCGTTTTTCCATGCGCTGTTGGATAACGGAATTTATGCGTCACCTAGTGTTTTTGAGACG
TGGTTTGTCTCTGCGGCGTTGACCGACGATGATTTTGACAAGATCGAAAAGGCGCTTCGCCCCGCAGCTCAAGCCGCAGG
AAAGGCAAAATGA

Upstream 100 bases:

>100_bases
ATTGATTCTGCAACTAAGGTGGCAAAGCTACTGGAAGATCACTGAGACGTGGCCGGCGTCGATAAGCGTTGTGATCTTCA
TGTCGGATAGGATGGTCGGC

Downstream 100 bases:

>100_bases
CAACTACTATTGTTCACCTCGTCCGCCACGGAGAGGTTCATAATCCTGAGCGTATTTTGTACGGCCGGATCCCTGATTAT
CACTTGTCCTCACGTGGCCG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MTSTQRSQELFARAQQFTPGGVNSPVRAFGSVGGHARFIQSAKGSKLYDVDGNEYVDLVCSWGPMLLGNAHPEIVEAVQK
AATNGLSFGAPTEAEVKIAEMIVDRTSVEEVRMVNSGTEATMSAVRLARGFTGRPKVIKFDGCYHGHVDALLASAGSGVA
TFALPDSPGVTGASAADTIVVPYNDSEAVRAAFEAHPDQIACVIAEAAAGNMGTVAPQDNFNAKLKEVAHEHGALLILDE
VMTGFRTSYNGWYGVDGVAGDLTTFGKVISGGLPAAAFGGRADIMRYLAPEGPVYQAGTLSGNPVAMAAGMKSLELATPE
VYDMVRANADRLAGLLHEALDREGVAHHIQRAATMLSVRFAEGEGHNFDDMKAADTFRFPAFFHALLDNGIYASPSVFET
WFVSAALTDDDFDKIEKALRPAAQAAGKAK

Sequences:

>Translated_430_residues
MTSTQRSQELFARAQQFTPGGVNSPVRAFGSVGGHARFIQSAKGSKLYDVDGNEYVDLVCSWGPMLLGNAHPEIVEAVQK
AATNGLSFGAPTEAEVKIAEMIVDRTSVEEVRMVNSGTEATMSAVRLARGFTGRPKVIKFDGCYHGHVDALLASAGSGVA
TFALPDSPGVTGASAADTIVVPYNDSEAVRAAFEAHPDQIACVIAEAAAGNMGTVAPQDNFNAKLKEVAHEHGALLILDE
VMTGFRTSYNGWYGVDGVAGDLTTFGKVISGGLPAAAFGGRADIMRYLAPEGPVYQAGTLSGNPVAMAAGMKSLELATPE
VYDMVRANADRLAGLLHEALDREGVAHHIQRAATMLSVRFAEGEGHNFDDMKAADTFRFPAFFHALLDNGIYASPSVFET
WFVSAALTDDDFDKIEKALRPAAQAAGKAK
>Mature_429_residues
TSTQRSQELFARAQQFTPGGVNSPVRAFGSVGGHARFIQSAKGSKLYDVDGNEYVDLVCSWGPMLLGNAHPEIVEAVQKA
ATNGLSFGAPTEAEVKIAEMIVDRTSVEEVRMVNSGTEATMSAVRLARGFTGRPKVIKFDGCYHGHVDALLASAGSGVAT
FALPDSPGVTGASAADTIVVPYNDSEAVRAAFEAHPDQIACVIAEAAAGNMGTVAPQDNFNAKLKEVAHEHGALLILDEV
MTGFRTSYNGWYGVDGVAGDLTTFGKVISGGLPAAAFGGRADIMRYLAPEGPVYQAGTLSGNPVAMAAGMKSLELATPEV
YDMVRANADRLAGLLHEALDREGVAHHIQRAATMLSVRFAEGEGHNFDDMKAADTFRFPAFFHALLDNGIYASPSVFETW
FVSAALTDDDFDKIEKALRPAAQAAGKAK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI37574042, Length=331, Percent_Identity=29.3051359516616, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI4557809, Length=376, Percent_Identity=26.5957446808511, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI24119277, Length=337, Percent_Identity=26.4094955489614, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI226442709, Length=303, Percent_Identity=29.7029702970297, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI226442705, Length=327, Percent_Identity=27.82874617737, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI13994255, Length=310, Percent_Identity=26.4516129032258, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI284507298, Length=298, Percent_Identity=26.8456375838926, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1786349, Length=419, Percent_Identity=52.2673031026253, Blast_Score=423, Evalue=1e-120,
Organism=Escherichia coli, GI1789016, Length=390, Percent_Identity=30.5128205128205, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1789759, Length=319, Percent_Identity=30.0940438871473, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788044, Length=318, Percent_Identity=30.5031446540881, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787560, Length=347, Percent_Identity=30.5475504322767, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI145693181, Length=369, Percent_Identity=26.2872628726287, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71992977, Length=355, Percent_Identity=27.887323943662, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25144271, Length=327, Percent_Identity=26.605504587156, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323470, Length=300, Percent_Identity=27, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324432, Length=325, Percent_Identity=25.2307692307692, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161085790, Length=350, Percent_Identity=25.1428571428571, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28574759, Length=350, Percent_Identity=25.1428571428571, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_CORDI (Q6NJJ2)

Other databases:

- EMBL:   BX248355
- RefSeq:   NP_938790.1
- ProteinModelPortal:   Q6NJJ2
- SMR:   Q6NJJ2
- GeneID:   2649362
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0409
- NMPDR:   fig|257309.1.peg.381
- HOGENOM:   HBG725944
- OMA:   DKVIKFE
- PhylomeDB:   Q6NJJ2
- ProtClustDB:   PRK00062
- BioCyc:   CDIP257309:DIP0409-MONOMER
- BRENDA:   5.4.3.8
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45362; Mature: 45231

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTQRSQELFARAQQFTPGGVNSPVRAFGSVGGHARFIQSAKGSKLYDVDGNEYVDLVC
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHCCCCCEEEECCCCCEEEEEE
SWGPMLLGNAHPEIVEAVQKAATNGLSFGAPTEAEVKIAEMIVDRTSVEEVRMVNSGTEA
CCCCCEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCHHH
TMSAVRLARGFTGRPKVIKFDGCYHGHVDALLASAGSGVATFALPDSPGVTGASAADTIV
HHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEE
VPYNDSEAVRAAFEAHPDQIACVIAEAAAGNMGTVAPQDNFNAKLKEVAHEHGALLILDE
EECCCCHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEEHHH
VMTGFRTSYNGWYGVDGVAGDLTTFGKVISGGLPAAAFGGRADIMRYLAPEGPVYQAGTL
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCHHHHHHHHCCCCCCEECCCC
SGNPVAMAAGMKSLELATPEVYDMVRANADRLAGLLHEALDREGVAHHIQRAATMLSVRF
CCCCEEEECCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEE
AEGEGHNFDDMKAADTFRFPAFFHALLDNGIYASPSVFETWFVSAALTDDDFDKIEKALR
ECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCHHHHHHHHHH
PAAQAAGKAK
HHHHHCCCCC
>Mature Secondary Structure 
TSTQRSQELFARAQQFTPGGVNSPVRAFGSVGGHARFIQSAKGSKLYDVDGNEYVDLVC
CCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHCCCCCEEEECCCCCEEEEEE
SWGPMLLGNAHPEIVEAVQKAATNGLSFGAPTEAEVKIAEMIVDRTSVEEVRMVNSGTEA
CCCCCEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCCHHH
TMSAVRLARGFTGRPKVIKFDGCYHGHVDALLASAGSGVATFALPDSPGVTGASAADTIV
HHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEE
VPYNDSEAVRAAFEAHPDQIACVIAEAAAGNMGTVAPQDNFNAKLKEVAHEHGALLILDE
EECCCCHHHHHHHHCCCCCEEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEEHHH
VMTGFRTSYNGWYGVDGVAGDLTTFGKVISGGLPAAAFGGRADIMRYLAPEGPVYQAGTL
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCCHHHHHHHHCCCCCCEECCCC
SGNPVAMAAGMKSLELATPEVYDMVRANADRLAGLLHEALDREGVAHHIQRAATMLSVRF
CCCCEEEECCCCHHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEE
AEGEGHNFDDMKAADTFRFPAFFHALLDNGIYASPSVFETWFVSAALTDDDFDKIEKALR
ECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHEECCCCHHHHHHHHHH
PAAQAAGKAK
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910