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The map label for this gene is pdxT

Identifier: 38232854

GI number: 38232854

Start: 194965

End: 195522

Strand: Direct

Name: pdxT

Synonym: DIP0228

Alternate gene names: 38232854

Gene position: 194965-195522 (Clockwise)

Preceding gene: 38232853

Following gene: 38232858

Centisome position: 7.83

GC content: 60.93

Gene sequence:

>558_bases
ATGGTAATCGGAGTACTCACCCTCCAGGGTGGGTTTGCAGAACACATTGCGATCCTAGAGTCCTTGGGTGTGGAGCACCG
TCGGGTGCGCGTGCCCAATGACCTGCTGGGGCTCGACGGCCTGATTATCCCAGGTGGCGAGTCCACTGTGATGGATAAGC
TGGCACGTGCTTTCGACCTTGCGGAGCCCCTGCGTGCTGCGATCAACAATGGTCTGCCGGTGTTTGCTACCTGTGCTGGG
TTGATTTACTTGGGCACGGTGGAAAATCCGGCGAAGGGCCAGCAGACGTTGGGCTGCTTGGACGTGGTGGTGCGCCGTAA
TGCTTTTGGCCGCCAGGTGGATTCCTTTGACGCGGTAGTTGACGTGGAAGGAATCGACGCGAATGTGGCCTTTATCCGCG
CCCCCGAGGTTATTTCCTGCGGGGCGGGGGTTACGGTGACCGCGCGTGTTGGCGACCATGTGGTGGGCGTGCGCCAGGGC
AAGATCCACGCCTATGCGTTCCACCCAGAATCCGCTGGTGAGGTCCGCTTGCACCAGGCGTGGCTCGCCAGCATCTAG

Upstream 100 bases:

>100_bases
ATCCTGCTGCTATCGCAGAGGTGTCTCGCGGCCTAGGCGAGGCAATGGTCGGCATTAACGTTGCCGATGTTCCTGCACCT
CACCGCTTGGCTGAGCGCGG

Downstream 100 bases:

>100_bases
TTGCTGCGCTGTGTCAAGTTGCTGCGCTGTGCTTTGCGACTGTTCTTGCGTAGCCACCAAGCAAGCAGGATCAGCACGAG
CGCGGCTGCGGCGACGATGG

Product: glutamine amidotransferase subunit PdxT

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MVIGVLTLQGGFAEHIAILESLGVEHRRVRVPNDLLGLDGLIIPGGESTVMDKLARAFDLAEPLRAAINNGLPVFATCAG
LIYLGTVENPAKGQQTLGCLDVVVRRNAFGRQVDSFDAVVDVEGIDANVAFIRAPEVISCGAGVTVTARVGDHVVGVRQG
KIHAYAFHPESAGEVRLHQAWLASI

Sequences:

>Translated_185_residues
MVIGVLTLQGGFAEHIAILESLGVEHRRVRVPNDLLGLDGLIIPGGESTVMDKLARAFDLAEPLRAAINNGLPVFATCAG
LIYLGTVENPAKGQQTLGCLDVVVRRNAFGRQVDSFDAVVDVEGIDANVAFIRAPEVISCGAGVTVTARVGDHVVGVRQG
KIHAYAFHPESAGEVRLHQAWLASI
>Mature_185_residues
MVIGVLTLQGGFAEHIAILESLGVEHRRVRVPNDLLGLDGLIIPGGESTVMDKLARAFDLAEPLRAAINNGLPVFATCAG
LIYLGTVENPAKGQQTLGCLDVVVRRNAFGRQVDSFDAVVDVEGIDANVAFIRAPEVISCGAGVTVTARVGDHVVGVRQG
KIHAYAFHPESAGEVRLHQAWLASI

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=204, Percent_Identity=39.7058823529412, Blast_Score=101, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6321048, Length=210, Percent_Identity=37.6190476190476, Blast_Score=100, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323995, Length=210, Percent_Identity=36.6666666666667, Blast_Score=99, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_CORDI (Q6NK10)

Other databases:

- EMBL:   BX248354
- RefSeq:   NP_938621.1
- ProteinModelPortal:   Q6NK10
- SMR:   Q6NK10
- GeneID:   2648728
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0228
- NMPDR:   fig|257309.1.peg.212
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- PhylomeDB:   Q6NK10
- ProtClustDB:   PRK13525
- BioCyc:   CDIP257309:DIP0228-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 19556; Mature: 19556

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 78-78 ACT_SITE 168-168 ACT_SITE 170-170 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIGVLTLQGGFAEHIAILESLGVEHRRVRVPNDLLGLDGLIIPGGESTVMDKLARAFDL
CEEEEEEECCCHHHHHHHHHHCCCCCEEEECCCHHCCCCCEEECCCCHHHHHHHHHHHHH
AEPLRAAINNGLPVFATCAGLIYLGTVENPAKGQQTLGCLDVVVRRNAFGRQVDSFDAVV
HHHHHHHHHCCCCHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEE
DVEGIDANVAFIRAPEVISCGAGVTVTARVGDHVVGVRQGKIHAYAFHPESAGEVRLHQA
EECCCCCCEEEEECCCEEECCCCCEEEEECCCEEEEEECCEEEEEEECCCCCCCEEEHHH
WLASI
HHHCC
>Mature Secondary Structure
MVIGVLTLQGGFAEHIAILESLGVEHRRVRVPNDLLGLDGLIIPGGESTVMDKLARAFDL
CEEEEEEECCCHHHHHHHHHHCCCCCEEEECCCHHCCCCCEEECCCCHHHHHHHHHHHHH
AEPLRAAINNGLPVFATCAGLIYLGTVENPAKGQQTLGCLDVVVRRNAFGRQVDSFDAVV
HHHHHHHHHCCCCHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEE
DVEGIDANVAFIRAPEVISCGAGVTVTARVGDHVVGVRQGKIHAYAFHPESAGEVRLHQA
EECCCCCCEEEEECCCEEECCCCCEEEEECCCEEEEEECCEEEEEEECCCCCCCEEEHHH
WLASI
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910