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The map label for this gene is lacA [C]

Identifier: 38232760

GI number: 38232760

Start: 112573

End: 113142

Strand: Reverse

Name: lacA [C]

Synonym: DIP0132

Alternate gene names: 38232760

Gene position: 113142-112573 (Counterclockwise)

Preceding gene: 38232765

Following gene: 38232759

Centisome position: 4.55

GC content: 58.07

Gene sequence:

>570_bases
ATGGCCGACCAGCGCGCCCAACGATGGCATATCCCGGTATCTCCGGAGATCACGCAGGCTATGGCTCGTACCGCCGAGTT
GGTTTTCGATTACAACGCCACTCACCCAGCGCATACCGAGGAGCTTAGTCGCCTCCGCCGGTTGATCCTCAGCCCCGCCA
GTAAGGACTGCACGATCAGACAGCCTCTGACCATTGAATATGGTGTTAACACGACCATTGGCAAAGACACTTTTATCAAT
TACGGCGTGACCATCCTCGACACCGCAGAGGTCACCATTGGCAGCCAGGTTCTTATCGGACCGAACTGCCAGCTCATCAC
GGTCACCCACCCCGTTGACAACGCCGATATGCGCACCGCCGGCTGGGAAATTGCCCACCCGATTGTCGTCGGCAAGCAGG
CATGGCTAGGGGCTGGCGTGATCGTTTTACCTGGGGTCACCATCGGCGAGCGTGCTGTTATCGGCGCTGGCAGTGTGGTC
ACCCACGATATTCCCGACGACACCATCGCCTACGGCAACCCCGCCCGAGTTATACGCACCACCAATCCTCACACCACTTA
TGAGCTTTAA

Upstream 100 bases:

>100_bases
CCGTGGCAGCTCACCACTACCCCAACCGCTATCACTGAAAAGATCTGGCATACCCACTAACCTAGCCATCATGAGCTTAG
ACCCGCATACCCCAGCCACG

Downstream 100 bases:

>100_bases
TCTTTCTGTTATCGGCGCCGGCCTGCCCGTCGCACAGTCCACCACGACGCTTATCGACGCCCTGCGCACCCATCGCATGG
CGGTGGTACAAGCCCCACCT

Product: putative sugar acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MADQRAQRWHIPVSPEITQAMARTAELVFDYNATHPAHTEELSRLRRLILSPASKDCTIRQPLTIEYGVNTTIGKDTFIN
YGVTILDTAEVTIGSQVLIGPNCQLITVTHPVDNADMRTAGWEIAHPIVVGKQAWLGAGVIVLPGVTIGERAVIGAGSVV
THDIPDDTIAYGNPARVIRTTNPHTTYEL

Sequences:

>Translated_189_residues
MADQRAQRWHIPVSPEITQAMARTAELVFDYNATHPAHTEELSRLRRLILSPASKDCTIRQPLTIEYGVNTTIGKDTFIN
YGVTILDTAEVTIGSQVLIGPNCQLITVTHPVDNADMRTAGWEIAHPIVVGKQAWLGAGVIVLPGVTIGERAVIGAGSVV
THDIPDDTIAYGNPARVIRTTNPHTTYEL
>Mature_188_residues
ADQRAQRWHIPVSPEITQAMARTAELVFDYNATHPAHTEELSRLRRLILSPASKDCTIRQPLTIEYGVNTTIGKDTFINY
GVTILDTAEVTIGSQVLIGPNCQLITVTHPVDNADMRTAGWEIAHPIVVGKQAWLGAGVIVLPGVTIGERAVIGAGSVVT
HDIPDDTIAYGNPARVIRTTNPHTTYEL

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=178, Percent_Identity=36.5168539325843, Blast_Score=131, Evalue=2e-32,
Organism=Escherichia coli, GI1786664, Length=166, Percent_Identity=32.5301204819277, Blast_Score=106, Evalue=9e-25,
Organism=Escherichia coli, GI1788367, Length=135, Percent_Identity=36.2962962962963, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788345, Length=174, Percent_Identity=32.7586206896552, Blast_Score=65, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322243, Length=166, Percent_Identity=38.5542168674699, Blast_Score=131, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 20542; Mature: 20410

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQRAQRWHIPVSPEITQAMARTAELVFDYNATHPAHTEELSRLRRLILSPASKDCTIR
CCCCCCCEEECCCCHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEC
QPLTIEYGVNTTIGKDTFINYGVTILDTAEVTIGSQVLIGPNCQLITVTHPVDNADMRTA
CCEEEEECCCCCCCCCCEEECCEEEEEECEEEECCEEEECCCCEEEEEECCCCCCCCCCC
GWEIAHPIVVGKQAWLGAGVIVLPGVTIGERAVIGAGSVVTHDIPDDTIAYGNPARVIRT
CCEEECEEEECCCHHHCCCEEEECCCEECCEEEEECCCEEEECCCCCCEECCCCCEEEEE
TNPHTTYEL
CCCCEEECC
>Mature Secondary Structure 
ADQRAQRWHIPVSPEITQAMARTAELVFDYNATHPAHTEELSRLRRLILSPASKDCTIR
CCCCCCEEECCCCHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEC
QPLTIEYGVNTTIGKDTFINYGVTILDTAEVTIGSQVLIGPNCQLITVTHPVDNADMRTA
CCEEEEECCCCCCCCCCEEECCEEEEEECEEEECCEEEECCCCEEEEEECCCCCCCCCCC
GWEIAHPIVVGKQAWLGAGVIVLPGVTIGERAVIGAGSVVTHDIPDDTIAYGNPARVIRT
CCEEECEEEECCCHHHCCCEEEECCCEECCEEEEECCCEEEECCCCCCEECCCCCEEEEE
TNPHTTYEL
CCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]