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The map label for this gene is bioB

Identifier: 38232735

GI number: 38232735

Start: 86342

End: 87394

Strand: Direct

Name: bioB

Synonym: DIP0105

Alternate gene names: 38232735

Gene position: 86342-87394 (Clockwise)

Preceding gene: 38232731

Following gene: 38232736

Centisome position: 3.47

GC content: 57.93

Gene sequence:

>1053_bases
ATGATTATCGCTAGGATTTTTGGACATCTACCAATTGCCACAACATGGGAGCATCACGTGACTGACATTCTAGAACTCGC
CCGCACCAAAGTGCTCAACAACGGCGAAGGCCTGAACAAAGAAGAAGTCCTCCAAGTACTACAACTCGACGAAGCACGCA
TCCCAGAGCTGCTCGAACTCGCCCACGAAGTACGCCTCAAATGGTGCGGCGAAGAAGTAGAAGTAGAAGGAATCATCTCC
CTCAAAACCGGCGGCTGCCCAGAAGACTGCCACTTCTGCTCCCAATCCGGACTCTTCGAATCCCCCGTGCGCTCCGCATG
GCTCGACATCGCAGGCCTCGTAGAGGCAGCAAAGCAAACCCAAAAATCCGGTGCCACCGAATTCTGCATCGTGGCAGCCG
TCAAAGGCCCCGACGAGCGTCTGATGAGCCAGCTTGAAGAAGCCGTCGCCGCTATCAAATCGGAAGTCGACATCGAAGTC
GCAGCCTCCATCGGCATCCTCACCCAAGAACAAGTCGACCGCCTCAAAGCCGCCGGCGTGCACCGCTACAACCACAACCT
AGAAACCGCACGCTCCTACTTCCCCAACGTAGTGACCACCCACTCCTGGGAATCCCGCCGCGAAACCCTACGCATGGTCG
GCGAAGCCGGAATGGAAGTCTGCTCCGGTGGCATCATCGGCATGGGTGAAACCCTAGAGCAGCGCGCCGAATTTGCCTGT
GACCTCGCAGAACTCAATCCCACCGAAGTTCCCATGAACTTCCTCGACCCACGCCCAGGAACCCCATTCGCGGACTATGA
AGTCCTCGACACCACAGATGCATTGCGCGCAATTGGAGCATTCCGACTTGCACTTCCTAAAACCATCCTGCGCTTCGCCG
GCGGCCGCGAACTCACCCTCGGCGACCTCGGCACCGAGCAAGGCCTCCTCGGTGGCATTAACGCCGTGATCGTGGGCAAC
TACCTGACCACCCTCGGCCGCCCCATGGAACAAGACCTCGACATGCTCGGCAAACTCCGCCTGCCTATCAAAGCCCTGAA
CGCGAGCGTCTAA

Upstream 100 bases:

>100_bases
ATGAGTTCGGTGAGCAGGGCGAGGGTGTCGTCGTAAAGCGTCATGTGTACCACATTAAGGGCAATGGGGGTGATTGACAT
CCAAGATTAAACACCGTTCA

Downstream 100 bases:

>100_bases
TGGCCACACACGCACAATCCACAGAGCTCCTCGAAGCCCTCCTCGCCGGCGAAGCACCTCGCTTTCAACCCAACACCGGC
CAAGAGCTTGTCGACGACAT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MIIARIFGHLPIATTWEHHVTDILELARTKVLNNGEGLNKEEVLQVLQLDEARIPELLELAHEVRLKWCGEEVEVEGIIS
LKTGGCPEDCHFCSQSGLFESPVRSAWLDIAGLVEAAKQTQKSGATEFCIVAAVKGPDERLMSQLEEAVAAIKSEVDIEV
AASIGILTQEQVDRLKAAGVHRYNHNLETARSYFPNVVTTHSWESRRETLRMVGEAGMEVCSGGIIGMGETLEQRAEFAC
DLAELNPTEVPMNFLDPRPGTPFADYEVLDTTDALRAIGAFRLALPKTILRFAGGRELTLGDLGTEQGLLGGINAVIVGN
YLTTLGRPMEQDLDMLGKLRLPIKALNASV

Sequences:

>Translated_350_residues
MIIARIFGHLPIATTWEHHVTDILELARTKVLNNGEGLNKEEVLQVLQLDEARIPELLELAHEVRLKWCGEEVEVEGIIS
LKTGGCPEDCHFCSQSGLFESPVRSAWLDIAGLVEAAKQTQKSGATEFCIVAAVKGPDERLMSQLEEAVAAIKSEVDIEV
AASIGILTQEQVDRLKAAGVHRYNHNLETARSYFPNVVTTHSWESRRETLRMVGEAGMEVCSGGIIGMGETLEQRAEFAC
DLAELNPTEVPMNFLDPRPGTPFADYEVLDTTDALRAIGAFRLALPKTILRFAGGRELTLGDLGTEQGLLGGINAVIVGN
YLTTLGRPMEQDLDMLGKLRLPIKALNASV
>Mature_350_residues
MIIARIFGHLPIATTWEHHVTDILELARTKVLNNGEGLNKEEVLQVLQLDEARIPELLELAHEVRLKWCGEEVEVEGIIS
LKTGGCPEDCHFCSQSGLFESPVRSAWLDIAGLVEAAKQTQKSGATEFCIVAAVKGPDERLMSQLEEAVAAIKSEVDIEV
AASIGILTQEQVDRLKAAGVHRYNHNLETARSYFPNVVTTHSWESRRETLRMVGEAGMEVCSGGIIGMGETLEQRAEFAC
DLAELNPTEVPMNFLDPRPGTPFADYEVLDTTDALRAIGAFRLALPKTILRFAGGRELTLGDLGTEQGLLGGINAVIVGN
YLTTLGRPMEQDLDMLGKLRLPIKALNASV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=302, Percent_Identity=36.7549668874172, Blast_Score=200, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6321725, Length=317, Percent_Identity=30.9148264984227, Blast_Score=165, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB1_CORDI (Q6NKC7)

Other databases:

- EMBL:   BX248354
- RefSeq:   NP_938502.1
- HSSP:   P12996
- ProteinModelPortal:   Q6NKC7
- SMR:   Q6NKC7
- GeneID:   2649072
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0105
- NMPDR:   fig|257309.1.peg.93
- HOGENOM:   HBG405569
- OMA:   ATGATEF
- PhylomeDB:   Q6NKC7
- ProtClustDB:   PRK06256
- BioCyc:   CDIP257309:DIP0105-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 38224; Mature: 38224

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIARIFGHLPIATTWEHHVTDILELARTKVLNNGEGLNKEEVLQVLQLDEARIPELLEL
CCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHH
AHEVRLKWCGEEVEVEGIISLKTGGCPEDCHFCSQSGLFESPVRSAWLDIAGLVEAAKQT
HHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
QKSGATEFCIVAAVKGPDERLMSQLEEAVAAIKSEVDIEVAASIGILTQEQVDRLKAAGV
HHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEHHHHCHHHHHHHHHHHHHHH
HRYNHNLETARSYFPNVVTTHSWESRRETLRMVGEAGMEVCSGGIIGMGETLEQRAEFAC
HHHCCCHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
DLAELNPTEVPMNFLDPRPGTPFADYEVLDTTDALRAIGAFRLALPKTILRFAGGRELTL
HHHHCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GDLGTEQGLLGGINAVIVGNYLTTLGRPMEQDLDMLGKLRLPIKALNASV
CCCCCCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure
MIIARIFGHLPIATTWEHHVTDILELARTKVLNNGEGLNKEEVLQVLQLDEARIPELLEL
CCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHH
AHEVRLKWCGEEVEVEGIISLKTGGCPEDCHFCSQSGLFESPVRSAWLDIAGLVEAAKQT
HHHHHHHHCCCEEEEEEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
QKSGATEFCIVAAVKGPDERLMSQLEEAVAAIKSEVDIEVAASIGILTQEQVDRLKAAGV
HHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEHHHHCHHHHHHHHHHHHHHH
HRYNHNLETARSYFPNVVTTHSWESRRETLRMVGEAGMEVCSGGIIGMGETLEQRAEFAC
HHHCCCHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
DLAELNPTEVPMNFLDPRPGTPFADYEVLDTTDALRAIGAFRLALPKTILRFAGGRELTL
HHHHCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
GDLGTEQGLLGGINAVIVGNYLTTLGRPMEQDLDMLGKLRLPIKALNASV
CCCCCCCCHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910