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The map label for this gene is yfiK [H]

Identifier: 38232721

GI number: 38232721

Start: 75820

End: 76479

Strand: Reverse

Name: yfiK [H]

Synonym: DIP0091

Alternate gene names: 38232721

Gene position: 76479-75820 (Counterclockwise)

Preceding gene: 38232722

Following gene: 38232720

Centisome position: 3.07

GC content: 58.03

Gene sequence:

>660_bases
ATGATTCGCATTGTGCTTGCCGACGACCAACCGCCCATCCTCAAAGCGTTCTCCACAATCATCCACTCCCGCGACACCAT
GCGCGTAGTGGCCACAGCCGCCAACGGCGCCGAAGCCATCAATGCGTGCATGAGCCACAACGTGGATGTTGTAGTCATGG
ATATCCGCATGCCCGTCATGGATGGCATCACCGCAGCACGCGCCATCCTCGCCCGGCACCCTCGCATCCGCATCGTCATG
CTCACCACTTTCGACGACCCCACGCTCGTCCGCGAAGCGCTCACCGCCGGAGTGCACGGATTTCTTCTTAAAGACGCCGA
CCCCGAAATCTTCCTCACCGCAATCGAGGCGATCCACTCAGGGGAATCCGTTCTAGCCAGCGGAGTAACCAAGCAGGTTA
TTGACACATTCCGCCACGCCTATGGCAGCGATCTCAGCCCTGAGCAGCGCCAAGGACTCAGCCTGATCACCCGGCGCGAA
ATGGATGTACTACTGTGCGTTGCACAAGGGCTCACCAACGCGGAGATCAGTGCTGACCTTGGGATTGCAGAAACGACCGT
AAAAACGCATATCACCGCACTACTAGCAAAGGTGCATGCCCGTGACCGCGTCGCTTTGGTGTTGTTTGTACATAAAGCTG
GGCTTCTCCTACCACGGTAG

Upstream 100 bases:

>100_bases
ACGAACGCGCAACCGCACTCGGCGGTCGTTTTAGTGCCCAAGGAACCGCATCCGATTTTCATGTTTGTGCCACCATTCCC
ATGCACCAAGAGGACCATTA

Downstream 100 bases:

>100_bases
GATCTGCGCTTGTGGTTGCTGGATAACAATCAACAACCATGAGTACAACTATTCGAGTCAATGAAGTCTCTAAAAGCTTC
CACGGCCAGCATGTTTTGAA

Product: putative two-component system response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MIRIVLADDQPPILKAFSTIIHSRDTMRVVATAANGAEAINACMSHNVDVVVMDIRMPVMDGITAARAILARHPRIRIVM
LTTFDDPTLVREALTAGVHGFLLKDADPEIFLTAIEAIHSGESVLASGVTKQVIDTFRHAYGSDLSPEQRQGLSLITRRE
MDVLLCVAQGLTNAEISADLGIAETTVKTHITALLAKVHARDRVALVLFVHKAGLLLPR

Sequences:

>Translated_219_residues
MIRIVLADDQPPILKAFSTIIHSRDTMRVVATAANGAEAINACMSHNVDVVVMDIRMPVMDGITAARAILARHPRIRIVM
LTTFDDPTLVREALTAGVHGFLLKDADPEIFLTAIEAIHSGESVLASGVTKQVIDTFRHAYGSDLSPEQRQGLSLITRRE
MDVLLCVAQGLTNAEISADLGIAETTVKTHITALLAKVHARDRVALVLFVHKAGLLLPR
>Mature_219_residues
MIRIVLADDQPPILKAFSTIIHSRDTMRVVATAANGAEAINACMSHNVDVVVMDIRMPVMDGITAARAILARHPRIRIVM
LTTFDDPTLVREALTAGVHGFLLKDADPEIFLTAIEAIHSGESVLASGVTKQVIDTFRHAYGSDLSPEQRQGLSLITRRE
MDVLLCVAQGLTNAEISADLGIAETTVKTHITALLAKVHARDRVALVLFVHKAGLLLPR

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=214, Percent_Identity=34.5794392523364, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1788222, Length=216, Percent_Identity=28.2407407407407, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787473, Length=209, Percent_Identity=31.1004784688995, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI1790102, Length=207, Percent_Identity=29.951690821256, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI1786747, Length=204, Percent_Identity=21.5686274509804, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1788546, Length=213, Percent_Identity=23.0046948356808, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788712, Length=208, Percent_Identity=22.1153846153846, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23740; Mature: 23740

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIVLADDQPPILKAFSTIIHSRDTMRVVATAANGAEAINACMSHNVDVVVMDIRMPVM
CEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHH
DGITAARAILARHPRIRIVMLTTFDDPTLVREALTAGVHGFLLKDADPEIFLTAIEAIHS
HHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHCHHEEEEECCCCHHHHHHHHHHHC
GESVLASGVTKQVIDTFRHAYGSDLSPEQRQGLSLITRREMDVLLCVAQGLTNAEISADL
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GIAETTVKTHITALLAKVHARDRVALVLFVHKAGLLLPR
CCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCC
>Mature Secondary Structure
MIRIVLADDQPPILKAFSTIIHSRDTMRVVATAANGAEAINACMSHNVDVVVMDIRMPVM
CEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHH
DGITAARAILARHPRIRIVMLTTFDDPTLVREALTAGVHGFLLKDADPEIFLTAIEAIHS
HHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHCHHEEEEECCCCHHHHHHHHHHHC
GESVLASGVTKQVIDTFRHAYGSDLSPEQRQGLSLITRREMDVLLCVAQGLTNAEISADL
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GIAETTVKTHITALLAKVHARDRVALVLFVHKAGLLLPR
CCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]