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The map label for this gene is thiC

Identifier: 38232671

GI number: 38232671

Start: 27567

End: 29366

Strand: Direct

Name: thiC

Synonym: DIP0029

Alternate gene names: 38232671

Gene position: 27567-29366 (Clockwise)

Preceding gene: 38232669

Following gene: 38232672

Centisome position: 1.11

GC content: 55.28

Gene sequence:

>1800_bases
GTGTCGGCAGCCTCAGCTAATTCAGTAACCAATCCATCTGCTTGGGAAAACTCCGAGATCCATCCGAAGCACTCGTATTC
GCCGATCGTTTCCGGTGACCTTGAGGTCCCAGAAACAGAGATTCAGCTTGACGATTCTCCTACCGGCCCCAATGATCCAG
TTCGGATCTATCGCACCCGTGGCCCTGAGTGTGATCCCACGGTGGGATTGAAGCCACTGCGTGCACAGTGGATTGATAGT
CGTGAGGACACGGAAGAATATGCTGGCCGTGAGCGGAATTTGGCTGATGATGGCCGCTCCGCGCAGCGTCGTGGCGCAGC
TTCGCTGGAGTGGAAAGGCGTGAAACCCACACCGCGACGCGCCAAGCAGGGCAAGCGCGTGACTCAGATGCACTATGCAC
GCCAGGGCATTATTACGAAGGAAATGGAGTTTGTGGCGCTGCGCGAGCACATGGACCCAGAGTTTGTGCGCAGCGAGATC
GCCCGTGGCCGTGCGATCATTCCGAACAACATTAACCACCCAGAATCGGAGCCCATGATTATTGGTCGAAAGTTTTTGAC
CAAGATCAACGCCAATATCGGTAACTCCGCAGTGACCTCCAGTATTGAGGAAGAAGTATCCAAGCTGCGGTGGGCTACCC
GCTGGGGTGCAGATACCGTGATGGATTTGTCCACCGGCGATGATATTCACACCACCCGCGAATGGATTATCCGCAACTCC
CCAGTACCAATCGGTACGGTGCCGATCTATCAAGCGCTGGAAAAAGTCAATGGTGTAGCAGAAGACCTTACGTGGGAGAT
TTTCCGCGATACCGTCATTGAGCAGTGTGAACAAGGTGTGGACTACATGACCATTCATGCCGGTGTGTTGCTCGCCTACA
TTCCACTGACCACCAAACGCATAACCGGAATTGTATCCCGTGGTGGATCCATCATGGCGGGTTGGTGCCTAGCTCACCAT
AAGGAGTCCTTCCTTTACGAGCACTTTGATGAGCTGTGCGAGATTTTCGCGCAGTACGACGTCGCCTTCTCGTTGGGTGA
TGGCCTGCGCCCTGGTTCTGTTGCCGATGCGAATGATGCGGCCCAGTTCGCGGAGCTGAAAACCATTGGTGAGCTGGCTC
GTCGTGCGTGGGAATACGATGTGCAGGTCATGATCGAGGGCCCAGGTCACGTTCCGCTGAACATGGTGCAGGAAAACAAT
GAGCTGGAGCAAAAGTGGGCTCACGATGCGCCTTTCTACACTCTTGGCCCATTGGTGACCGATATTGCGCCAGGCTATGA
CCACATCACCTCGGCAATTGGAGCTGCTCATATCGCTATGGGTGGTACGGCGATGCTGTGTTATGTGACCCCGAAGGAGC
ACTTGGGTCTGCCCAACCGTGACGATGTAAAAACCGGTGTGATTACTTATAAACTCGCAGCTCACGCGGCCGATGTGGCT
AAGGGGCACCCTGGTGCTCGTGCGTGGGATGACGCGATGAGCAAGGCACGTTTTGAGTTCCGTTGGCACGATCAGTTCGC
GTTGTCGCTTGATCCAGATACCGCGATTGCTTACCATGACGAAACGCTTCCGGCGGAGCCGGCAAAGACCGCGCACTTCT
GTTCCATGTGTGGTCCGAAGTTCTGCTCCATGCGCATTAGCCAAGATATTCGTGACATGTTCGCAGATAAGATCGCCGAC
TTGGGTATCCCGCAGGTTGGCGGCGACGCAGAAGCTGGTATGGCGGCAAAGTCTGAGGAGTTTGTGGCCCAAGGATCGCA
GCTTTATAGCGAGGTGCGTGACAATGCTGCCCACGCCTAG

Upstream 100 bases:

>100_bases
CGGGCGCCCGAGCACGGGCTGAGATTGCGCTGATGCTGTGCAAGCACCGTTTGAACCTGTCTGGTTAACACCAGCGAAGG
AAGAGAGGAGCGCGAGCCTA

Downstream 100 bases:

>100_bases
GTGGGGTCGAGACTTTGATCCGCGGTGCTACTTTGTCACCGGCACGGGCTCTGTGGATCACATTGTCGACGTAGCGCGCC
AAGCAGCGCGCGCCGGCGCT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MSAASANSVTNPSAWENSEIHPKHSYSPIVSGDLEVPETEIQLDDSPTGPNDPVRIYRTRGPECDPTVGLKPLRAQWIDS
REDTEEYAGRERNLADDGRSAQRRGAASLEWKGVKPTPRRAKQGKRVTQMHYARQGIITKEMEFVALREHMDPEFVRSEI
ARGRAIIPNNINHPESEPMIIGRKFLTKINANIGNSAVTSSIEEEVSKLRWATRWGADTVMDLSTGDDIHTTREWIIRNS
PVPIGTVPIYQALEKVNGVAEDLTWEIFRDTVIEQCEQGVDYMTIHAGVLLAYIPLTTKRITGIVSRGGSIMAGWCLAHH
KESFLYEHFDELCEIFAQYDVAFSLGDGLRPGSVADANDAAQFAELKTIGELARRAWEYDVQVMIEGPGHVPLNMVQENN
ELEQKWAHDAPFYTLGPLVTDIAPGYDHITSAIGAAHIAMGGTAMLCYVTPKEHLGLPNRDDVKTGVITYKLAAHAADVA
KGHPGARAWDDAMSKARFEFRWHDQFALSLDPDTAIAYHDETLPAEPAKTAHFCSMCGPKFCSMRISQDIRDMFADKIAD
LGIPQVGGDAEAGMAAKSEEFVAQGSQLYSEVRDNAAHA

Sequences:

>Translated_599_residues
MSAASANSVTNPSAWENSEIHPKHSYSPIVSGDLEVPETEIQLDDSPTGPNDPVRIYRTRGPECDPTVGLKPLRAQWIDS
REDTEEYAGRERNLADDGRSAQRRGAASLEWKGVKPTPRRAKQGKRVTQMHYARQGIITKEMEFVALREHMDPEFVRSEI
ARGRAIIPNNINHPESEPMIIGRKFLTKINANIGNSAVTSSIEEEVSKLRWATRWGADTVMDLSTGDDIHTTREWIIRNS
PVPIGTVPIYQALEKVNGVAEDLTWEIFRDTVIEQCEQGVDYMTIHAGVLLAYIPLTTKRITGIVSRGGSIMAGWCLAHH
KESFLYEHFDELCEIFAQYDVAFSLGDGLRPGSVADANDAAQFAELKTIGELARRAWEYDVQVMIEGPGHVPLNMVQENN
ELEQKWAHDAPFYTLGPLVTDIAPGYDHITSAIGAAHIAMGGTAMLCYVTPKEHLGLPNRDDVKTGVITYKLAAHAADVA
KGHPGARAWDDAMSKARFEFRWHDQFALSLDPDTAIAYHDETLPAEPAKTAHFCSMCGPKFCSMRISQDIRDMFADKIAD
LGIPQVGGDAEAGMAAKSEEFVAQGSQLYSEVRDNAAHA
>Mature_598_residues
SAASANSVTNPSAWENSEIHPKHSYSPIVSGDLEVPETEIQLDDSPTGPNDPVRIYRTRGPECDPTVGLKPLRAQWIDSR
EDTEEYAGRERNLADDGRSAQRRGAASLEWKGVKPTPRRAKQGKRVTQMHYARQGIITKEMEFVALREHMDPEFVRSEIA
RGRAIIPNNINHPESEPMIIGRKFLTKINANIGNSAVTSSIEEEVSKLRWATRWGADTVMDLSTGDDIHTTREWIIRNSP
VPIGTVPIYQALEKVNGVAEDLTWEIFRDTVIEQCEQGVDYMTIHAGVLLAYIPLTTKRITGIVSRGGSIMAGWCLAHHK
ESFLYEHFDELCEIFAQYDVAFSLGDGLRPGSVADANDAAQFAELKTIGELARRAWEYDVQVMIEGPGHVPLNMVQENNE
LEQKWAHDAPFYTLGPLVTDIAPGYDHITSAIGAAHIAMGGTAMLCYVTPKEHLGLPNRDDVKTGVITYKLAAHAADVAK
GHPGARAWDDAMSKARFEFRWHDQFALSLDPDTAIAYHDETLPAEPAKTAHFCSMCGPKFCSMRISQDIRDMFADKIADL
GIPQVGGDAEAGMAAKSEEFVAQGSQLYSEVRDNAAHA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=603, Percent_Identity=63.681592039801, Blast_Score=761, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_CORDI (P61424)

Other databases:

- EMBL:   BX248354
- RefSeq:   NP_938438.1
- GeneID:   2650613
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0029
- NMPDR:   fig|257309.1.peg.29
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   P61424
- ProtClustDB:   PRK09284
- BioCyc:   CDIP257309:DIP0029-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 66204; Mature: 66073

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: BINDING 192-192 BINDING 221-221 BINDING 250-250 BINDING 286-286 BINDING 386-386 BINDING 413-413

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAASANSVTNPSAWENSEIHPKHSYSPIVSGDLEVPETEIQLDDSPTGPNDPVRIYRTR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECC
GPECDPTVGLKPLRAQWIDSREDTEEYAGRERNLADDGRSAQRRGAASLEWKGVKPTPRR
CCCCCCCCCCCHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHHCCCCCCEECCCCCCHHH
AKQGKRVTQMHYARQGIITKEMEFVALREHMDPEFVRSEIARGRAIIPNNINHPESEPMI
HHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEECCCCCCCCCCCCEE
IGRKFLTKINANIGNSAVTSSIEEEVSKLRWATRWGADTVMDLSTGDDIHTTREWIIRNS
EHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHEECC
PVPIGTVPIYQALEKVNGVAEDLTWEIFRDTVIEQCEQGVDYMTIHAGVLLAYIPLTTKR
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
ITGIVSRGGSIMAGWCLAHHKESFLYEHFDELCEIFAQYDVAFSLGDGLRPGSVADANDA
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHH
AQFAELKTIGELARRAWEYDVQVMIEGPGHVPLNMVQENNELEQKWAHDAPFYTLGPLVT
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHECCCCHHHHHHCCCCCCEEHHHHHH
DIAPGYDHITSAIGAAHIAMGGTAMLCYVTPKEHLGLPNRDDVKTGVITYKLAAHAADVA
HCCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCCCCCCCCEEEEHHHHHHHHHH
KGHPGARAWDDAMSKARFEFRWHDQFALSLDPDTAIAYHDETLPAEPAKTAHFCSMCGPK
CCCCCCHHHHHHHHHHEEEEEECCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHCCHH
FCSMRISQDIRDMFADKIADLGIPQVGGDAEAGMAAKSEEFVAQGSQLYSEVRDNAAHA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAASANSVTNPSAWENSEIHPKHSYSPIVSGDLEVPETEIQLDDSPTGPNDPVRIYRTR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECC
GPECDPTVGLKPLRAQWIDSREDTEEYAGRERNLADDGRSAQRRGAASLEWKGVKPTPRR
CCCCCCCCCCCHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHHCCCCCCEECCCCCCHHH
AKQGKRVTQMHYARQGIITKEMEFVALREHMDPEFVRSEIARGRAIIPNNINHPESEPMI
HHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEECCCCCCCCCCCCEE
IGRKFLTKINANIGNSAVTSSIEEEVSKLRWATRWGADTVMDLSTGDDIHTTREWIIRNS
EHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHEECC
PVPIGTVPIYQALEKVNGVAEDLTWEIFRDTVIEQCEQGVDYMTIHAGVLLAYIPLTTKR
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHH
ITGIVSRGGSIMAGWCLAHHKESFLYEHFDELCEIFAQYDVAFSLGDGLRPGSVADANDA
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCHHH
AQFAELKTIGELARRAWEYDVQVMIEGPGHVPLNMVQENNELEQKWAHDAPFYTLGPLVT
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHECCCCHHHHHHCCCCCCEEHHHHHH
DIAPGYDHITSAIGAAHIAMGGTAMLCYVTPKEHLGLPNRDDVKTGVITYKLAAHAADVA
HCCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHCCCCCCCCCCCCEEEEHHHHHHHHHH
KGHPGARAWDDAMSKARFEFRWHDQFALSLDPDTAIAYHDETLPAEPAKTAHFCSMCGPK
CCCCCCHHHHHHHHHHEEEEEECCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHCCHH
FCSMRISQDIRDMFADKIADLGIPQVGGDAEAGMAAKSEEFVAQGSQLYSEVRDNAAHA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910