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The map label for this gene is recF [H]

Identifier: 21675093

GI number: 21675093

Start: 2153826

End: 2154932

Strand: Reverse

Name: recF [H]

Synonym: CT2288

Alternate gene names: 21675093

Gene position: 2154932-2153826 (Counterclockwise)

Preceding gene: NA

Following gene: 21675092

Centisome position: 100.0

GC content: 44.35

Gene sequence:

>1107_bases
TTGCGACTCGACTCCATCAGTATCGCCAATTTCAGGAATCACACGCTTCTTGAATTCGAGCCAGGCCACTCCATTACCAA
TATTTATGGCAGAAATGGTTCGGGAAAAACATCGATTCTTGAGGCGATCCATTATTGCGCTCTGACAAGAGGCTTTTCCG
GAAATAACGATCGGGAGTATCTGAAGTTCGGGGAGGAGCTGTTTACCATCCGCTCCTCATTTACGAGTGGTCAGGGAATT
GCTACTAAGGTTTCGATCACCTATTCACCGAAGAGGGAGAAGAGAATCCTTGTTAACGAACAGGAGCTTCAAACCTTTTC
CAGCCATATCGGGACCATTCCGTGCGTGACCTTTACGCCACGTGAGATGGTGATCATCAACGGCGCACCGGCTGAGCGTC
GTCGATTCATCGATACGGCAATTTGTCAGTATGATCGGAAGTATTTATCCGATTTATTGCTGTATAGGCGTATTTTACAG
CAACGTAATGCCCTGCTTTCTTCGGAGCAGGATCCACGTGTTATTGATTCAGCTCTCGATGTTCTAACCGATCAACTCGT
TGCTATAGCAACGGAAATTGTTCTGGTAAGAAAGCGATTTATCGAGCATTTTACTTCCATGCTTGGTGGTGTCTATCAAT
GGATCCCTGAAGGAGCTGAGCCATCAATTTTATATCAAAGTTCTCTTGGTCATCACGAAAATCTGTATGAAAAAGATAAG
ATACAGCAGGTTTTTAGAGAGCGTTTTGAAACGTTAAAGCAGCAGGAGTTACAACGAAGGCAAACGCTTGCTGGACCACA
TCGGGATGATCTGCAATTTTATCTAAACAAAAGAGAAATACGAAAATATGCCTCTCAGGGACAGCAGCGTGCATTTCTTG
TTGCTATGAAAATGACACTGCAAGGCTATCTTTATGAAGCATCGGGTGAGATACCAATTACCCTTCTTGACGATCTGTTC
AGTGAGCTTGATGAGGTGGTTTCCGGTACCATGGTCGAGACGCTTGCAACCAAAGGACAGGTGATCATCACCTCAACGGA
GAAAAAAAAGGGGAAGGGTATAAGTTTTTTTTCTGTTGATGATTACAAGAGTAGTAAGGAGCCTTGA

Upstream 100 bases:

>100_bases
AACGGCTGTGTTGTTCAAACCGAAATACGAGGAGCGCCAGGACGATCTGATCATTCTGGTGATGCCGGTCAGAATCAACA
ATTGACGGAACAGGAGAGGC

Downstream 100 bases:

>100_bases
TCATGAGTAAAGCAAAACCACCGAGAGAGCTTGGCGGGATCATAGCTGATGTTTTCTGTAAGATCGGCATGACGGAAGCC
TATGATGAATACAAGACACT

Product: recombination/replication protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MRLDSISIANFRNHTLLEFEPGHSITNIYGRNGSGKTSILEAIHYCALTRGFSGNNDREYLKFGEELFTIRSSFTSGQGI
ATKVSITYSPKREKRILVNEQELQTFSSHIGTIPCVTFTPREMVIINGAPAERRRFIDTAICQYDRKYLSDLLLYRRILQ
QRNALLSSEQDPRVIDSALDVLTDQLVAIATEIVLVRKRFIEHFTSMLGGVYQWIPEGAEPSILYQSSLGHHENLYEKDK
IQQVFRERFETLKQQELQRRQTLAGPHRDDLQFYLNKREIRKYASQGQQRAFLVAMKMTLQGYLYEASGEIPITLLDDLF
SELDEVVSGTMVETLATKGQVIITSTEKKKGKGISFFSVDDYKSSKEP

Sequences:

>Translated_368_residues
MRLDSISIANFRNHTLLEFEPGHSITNIYGRNGSGKTSILEAIHYCALTRGFSGNNDREYLKFGEELFTIRSSFTSGQGI
ATKVSITYSPKREKRILVNEQELQTFSSHIGTIPCVTFTPREMVIINGAPAERRRFIDTAICQYDRKYLSDLLLYRRILQ
QRNALLSSEQDPRVIDSALDVLTDQLVAIATEIVLVRKRFIEHFTSMLGGVYQWIPEGAEPSILYQSSLGHHENLYEKDK
IQQVFRERFETLKQQELQRRQTLAGPHRDDLQFYLNKREIRKYASQGQQRAFLVAMKMTLQGYLYEASGEIPITLLDDLF
SELDEVVSGTMVETLATKGQVIITSTEKKKGKGISFFSVDDYKSSKEP
>Mature_368_residues
MRLDSISIANFRNHTLLEFEPGHSITNIYGRNGSGKTSILEAIHYCALTRGFSGNNDREYLKFGEELFTIRSSFTSGQGI
ATKVSITYSPKREKRILVNEQELQTFSSHIGTIPCVTFTPREMVIINGAPAERRRFIDTAICQYDRKYLSDLLLYRRILQ
QRNALLSSEQDPRVIDSALDVLTDQLVAIATEIVLVRKRFIEHFTSMLGGVYQWIPEGAEPSILYQSSLGHHENLYEKDK
IQQVFRERFETLKQQELQRRQTLAGPHRDDLQFYLNKREIRKYASQGQQRAFLVAMKMTLQGYLYEASGEIPITLLDDLF
SELDEVVSGTMVETLATKGQVIITSTEKKKGKGISFFSVDDYKSSKEP

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=359, Percent_Identity=24.5125348189415, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 42118; Mature: 42118

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00617 RECF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDSISIANFRNHTLLEFEPGHSITNIYGRNGSGKTSILEAIHYCALTRGFSGNNDREY
CCCCCEEECCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHH
LKFGEELFTIRSSFTSGQGIATKVSITYSPKREKRILVNEQELQTFSSHIGTIPCVTFTP
HHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEECCHHHHHHHHHHCCCCCEEEECC
REMVIINGAPAERRRFIDTAICQYDRKYLSDLLLYRRILQQRNALLSSEQDPRVIDSALD
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VLTDQLVAIATEIVLVRKRFIEHFTSMLGGVYQWIPEGAEPSILYQSSLGHHENLYEKDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHH
IQQVFRERFETLKQQELQRRQTLAGPHRDDLQFYLNKREIRKYASQGQQRAFLVAMKMTL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QGYLYEASGEIPITLLDDLFSELDEVVSGTMVETLATKGQVIITSTEKKKGKGISFFSVD
HHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEC
DYKSSKEP
CCCCCCCC
>Mature Secondary Structure
MRLDSISIANFRNHTLLEFEPGHSITNIYGRNGSGKTSILEAIHYCALTRGFSGNNDREY
CCCCCEEECCCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHH
LKFGEELFTIRSSFTSGQGIATKVSITYSPKREKRILVNEQELQTFSSHIGTIPCVTFTP
HHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEEECCHHHHHHHHHHCCCCCEEEECC
REMVIINGAPAERRRFIDTAICQYDRKYLSDLLLYRRILQQRNALLSSEQDPRVIDSALD
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
VLTDQLVAIATEIVLVRKRFIEHFTSMLGGVYQWIPEGAEPSILYQSSLGHHENLYEKDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHH
IQQVFRERFETLKQQELQRRQTLAGPHRDDLQFYLNKREIRKYASQGQQRAFLVAMKMTL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QGYLYEASGEIPITLLDDLFSELDEVVSGTMVETLATKGQVIITSTEKKKGKGISFFSVD
HHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEC
DYKSSKEP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA