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The map label for this gene is rpoA [H]

Identifier: 21674971

GI number: 21674971

Start: 2049746

End: 2050732

Strand: Reverse

Name: rpoA [H]

Synonym: CT2162

Alternate gene names: 21674971

Gene position: 2050732-2049746 (Counterclockwise)

Preceding gene: 21674972

Following gene: 21674970

Centisome position: 95.16

GC content: 52.08

Gene sequence:

>987_bases
ATGATATATCAGATGCAGATGCCTACAAAGATCGATGTGGACGAAGCGACCCACACCGGCAGCTTCGGGCGATTCATAGC
CCAGCCGCTGGAGAGGGGATACGGAGTTACGCTGGGCAATGCCATGAGAAGGGTTCTGCTTGCCTCTCTTCCTGGAACAG
CAATCACCGGGATCAAGATCGATGGTGTGTTTCACGAGTTTTCAACGATTGACGGCGTTCGCGAGGATGTGCCCGAAATT
GTACTCAATCTGAAAAAAGTGCGCTTCAAGTCGAACTGCAAGCGCAGCTGCAAAACAACCCTGACCCTTGCCGGGCCGAA
AGATTTCCTTGCTGGCGACATCGTTGCACAGGAAGGTGAATTCGAGGTGCTCAACAAGGATCTGCATATCGCGACGATCA
ACTCCGAGGCTACCGTGACAATCGATATCTATATCGGCCGCGGGCGTGGTTATGTGCCCGCCGAAGAGAATCGCAGCGAC
GGAATGCCGATTGGCTTCATCGCCATCGACTCGATTTATACACCGATCAAGAACGTCAAACTGACCGTCGAGAACACCCG
TGTCGGCCAGAAGACCGACTACGAAAAGATGATTCTCGATGTCGAAACCGACGGCTCGATCACTCCCGATGACGCGATCA
GCCTCGCCGGCAAGATCATCAACGATCATATCACCTTTTTTGCCAACTTCTCTCCGACTGAAGAGGAGTTCTCCGAGGAG
GAGTACAAACAGCTCGACGACGAGTTTGAAAGCATGCGCAAGCTCCTGCAGACTAAAATCGAGGACCTCGATCTTTCGGT
TCGCTCGCACAACTGCCTGAGGCTCGCCGAAATCGATTCGCTTGGTGACTTGGTCTCCAGAAGGGAAGAGGAGCTGCTCA
ACTACAAAAATTTTGGCAAGAAGTCGCTGACCGAACTGAAGGAACAGCTTGAAAAGTTTAACCTCAAGTTCGGGATGGAT
ATCACCCGTTACCAGCTAAAGGGTTGA

Upstream 100 bases:

>100_bases
CTATTCCTGAGCGCGAAGCCGTGCAGGAGCCGTTCAACGAACAGCTTGTCGTCGAGTTGTACTCCAAGTGATCTTTTTGA
ATTCCTAAGGGAGCAAGACT

Downstream 100 bases:

>100_bases
GAAGGGTTCTTTGTTTTATTGAACATCAATGAGAGCGAAAACAAGAAATGCGTAAAGTCAAGCCAGCAAGAAAACTCGGA
AGAACGAGTGCCCATAGAAA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MIYQMQMPTKIDVDEATHTGSFGRFIAQPLERGYGVTLGNAMRRVLLASLPGTAITGIKIDGVFHEFSTIDGVREDVPEI
VLNLKKVRFKSNCKRSCKTTLTLAGPKDFLAGDIVAQEGEFEVLNKDLHIATINSEATVTIDIYIGRGRGYVPAEENRSD
GMPIGFIAIDSIYTPIKNVKLTVENTRVGQKTDYEKMILDVETDGSITPDDAISLAGKIINDHITFFANFSPTEEEFSEE
EYKQLDDEFESMRKLLQTKIEDLDLSVRSHNCLRLAEIDSLGDLVSRREEELLNYKNFGKKSLTELKEQLEKFNLKFGMD
ITRYQLKG

Sequences:

>Translated_328_residues
MIYQMQMPTKIDVDEATHTGSFGRFIAQPLERGYGVTLGNAMRRVLLASLPGTAITGIKIDGVFHEFSTIDGVREDVPEI
VLNLKKVRFKSNCKRSCKTTLTLAGPKDFLAGDIVAQEGEFEVLNKDLHIATINSEATVTIDIYIGRGRGYVPAEENRSD
GMPIGFIAIDSIYTPIKNVKLTVENTRVGQKTDYEKMILDVETDGSITPDDAISLAGKIINDHITFFANFSPTEEEFSEE
EYKQLDDEFESMRKLLQTKIEDLDLSVRSHNCLRLAEIDSLGDLVSRREEELLNYKNFGKKSLTELKEQLEKFNLKFGMD
ITRYQLKG
>Mature_328_residues
MIYQMQMPTKIDVDEATHTGSFGRFIAQPLERGYGVTLGNAMRRVLLASLPGTAITGIKIDGVFHEFSTIDGVREDVPEI
VLNLKKVRFKSNCKRSCKTTLTLAGPKDFLAGDIVAQEGEFEVLNKDLHIATINSEATVTIDIYIGRGRGYVPAEENRSD
GMPIGFIAIDSIYTPIKNVKLTVENTRVGQKTDYEKMILDVETDGSITPDDAISLAGKIINDHITFFANFSPTEEEFSEE
EYKQLDDEFESMRKLLQTKIEDLDLSVRSHNCLRLAEIDSLGDLVSRREEELLNYKNFGKKSLTELKEQLEKFNLKFGMD
ITRYQLKG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=321, Percent_Identity=38.6292834890966, Blast_Score=228, Evalue=5e-61,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 36937; Mature: 36937

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYQMQMPTKIDVDEATHTGSFGRFIAQPLERGYGVTLGNAMRRVLLASLPGTAITGIKI
CEEEECCCCEECCCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCEEEEEEE
DGVFHEFSTIDGVREDVPEIVLNLKKVRFKSNCKRSCKTTLTLAGPKDFLAGDIVAQEGE
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHEECCCC
FEVLNKDLHIATINSEATVTIDIYIGRGRGYVPAEENRSDGMPIGFIAIDSIYTPIKNVK
CEEECCCEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCEE
LTVENTRVGQKTDYEKMILDVETDGSITPDDAISLAGKIINDHITFFANFSPTEEEFSEE
EEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHH
EYKQLDDEFESMRKLLQTKIEDLDLSVRSHNCLRLAEIDSLGDLVSRREEELLNYKNFGK
HHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCH
KSLTELKEQLEKFNLKFGMDITRYQLKG
HHHHHHHHHHHHCCCCCCCCEEEEEECC
>Mature Secondary Structure
MIYQMQMPTKIDVDEATHTGSFGRFIAQPLERGYGVTLGNAMRRVLLASLPGTAITGIKI
CEEEECCCCEECCCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCEEEEEEE
DGVFHEFSTIDGVREDVPEIVLNLKKVRFKSNCKRSCKTTLTLAGPKDFLAGDIVAQEGE
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHEECCCC
FEVLNKDLHIATINSEATVTIDIYIGRGRGYVPAEENRSDGMPIGFIAIDSIYTPIKNVK
CEEECCCEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCEE
LTVENTRVGQKTDYEKMILDVETDGSITPDDAISLAGKIINDHITFFANFSPTEEEFSEE
EEEECCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHH
EYKQLDDEFESMRKLLQTKIEDLDLSVRSHNCLRLAEIDSLGDLVSRREEELLNYKNFGK
HHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCH
KSLTELKEQLEKFNLKFGMDITRYQLKG
HHHHHHHHHHHHCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA