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The map label for this gene is pheS [H]

Identifier: 21674939

GI number: 21674939

Start: 2016639

End: 2017664

Strand: Direct

Name: pheS [H]

Synonym: CT2130

Alternate gene names: 21674939

Gene position: 2016639-2017664 (Clockwise)

Preceding gene: 21674938

Following gene: 21674948

Centisome position: 93.58

GC content: 60.53

Gene sequence:

>1026_bases
ATGGAGACAAGCATTCAGCAACTGCAGCAGGAGATTGACGGATACCAGATCCGGAACGCAAAGGAACTCGAGGCCTTCAA
GCTCGAGTTCACGGTGCGTAAGGGCAAAATCGCCGGCCTGTTCGGCCAGCTCAAAACCGTCGATTCCGCTGACAGGCCTC
GCATAGGGCAGTTGCTCAACGCGCTCAAGCTCTCGGCAGAAGCGAAAGTTGCCGATGCCGAAAGCCTCTTCGCGCAAAAC
GCGGAGACTGAGGCACCAGCACTCGACCTCTCCCTGCCGGGACGCCGCTCGTTCCTCGGCTCGGAACATCCCGTGCAGAA
GGTGCTGGGCGACATGAAGCGCATCTTCACCGCGATGGGGTTCGGCATCGCCACCGGCCCGGAGCTTGAACTCGACGAGT
ATAACTTCGACCGCCTGAACTTTCCGCCGAACCACCCGGCCCGCGACATGCAGGACACCTTCTTCATCACGCGAGGCCAG
GAAGAGGGGGACGTGCTGCTCCGCACGCACACCTCACCGGTACAGGTGCGGGTGATGCTCGACGAGAAGCCGCCGATTCG
GGTGATCTGCCCCGGCAAGGTGTATCGCAACGAAGCGATCAGCGCACGGAGCTACTGCGTCTTCCACCAGCTCGAAGGCC
TGTACATCGACAAAAACGTGTCGTTCGCCGACCTGAAGGCCACAATCTACTCGTTCGCCCGCCAGATGTTCGGCAGCGAC
GTGAAGCTCAGATTCCGACCGAGCTTTTTCCCCTTCACCGAACCATCGGCTGAAGTGGACGTCACCTGCTACCTCTGTGG
TGGTAAGGGATGCCGCGTCTGCAAGAAGTCCGGCTGGCTCGAAATCATGGGCTGCGGCATGGTGCATCCGAACGTGATGC
GCAACTGCGGCATCGATCCGGAGGAGTGGACCGGCTACGCCTTCGGCATGGGGGTCGATCGCACGGCGCTGTTGCGCTAC
AAGATCGACGACATCCGGCTCTTTTTCGAAAACGACGTCCGGATGCTCAGCCAGTTCATGGCGTGA

Upstream 100 bases:

>100_bases
ATCGACCGCAAGGCGCTGGCCGAAATCGCCGTGAAGGATCCGGCAGCATTCTCTCTGATCGTCAAAACGGCTCTCGATTA
ATATTTTGTACAACGGCCCT

Downstream 100 bases:

>100_bases
AGCCTGGTTTGATAGAATAAAAAAGCCTCGTGAACATTGCGCTCGCGAGGCTTTTTCATTTCCGTCAGACTCCTGAATCA
GGCGGACACGGGGTTTTTCA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
METSIQQLQQEIDGYQIRNAKELEAFKLEFTVRKGKIAGLFGQLKTVDSADRPRIGQLLNALKLSAEAKVADAESLFAQN
AETEAPALDLSLPGRRSFLGSEHPVQKVLGDMKRIFTAMGFGIATGPELELDEYNFDRLNFPPNHPARDMQDTFFITRGQ
EEGDVLLRTHTSPVQVRVMLDEKPPIRVICPGKVYRNEAISARSYCVFHQLEGLYIDKNVSFADLKATIYSFARQMFGSD
VKLRFRPSFFPFTEPSAEVDVTCYLCGGKGCRVCKKSGWLEIMGCGMVHPNVMRNCGIDPEEWTGYAFGMGVDRTALLRY
KIDDIRLFFENDVRMLSQFMA

Sequences:

>Translated_341_residues
METSIQQLQQEIDGYQIRNAKELEAFKLEFTVRKGKIAGLFGQLKTVDSADRPRIGQLLNALKLSAEAKVADAESLFAQN
AETEAPALDLSLPGRRSFLGSEHPVQKVLGDMKRIFTAMGFGIATGPELELDEYNFDRLNFPPNHPARDMQDTFFITRGQ
EEGDVLLRTHTSPVQVRVMLDEKPPIRVICPGKVYRNEAISARSYCVFHQLEGLYIDKNVSFADLKATIYSFARQMFGSD
VKLRFRPSFFPFTEPSAEVDVTCYLCGGKGCRVCKKSGWLEIMGCGMVHPNVMRNCGIDPEEWTGYAFGMGVDRTALLRY
KIDDIRLFFENDVRMLSQFMA
>Mature_341_residues
METSIQQLQQEIDGYQIRNAKELEAFKLEFTVRKGKIAGLFGQLKTVDSADRPRIGQLLNALKLSAEAKVADAESLFAQN
AETEAPALDLSLPGRRSFLGSEHPVQKVLGDMKRIFTAMGFGIATGPELELDEYNFDRLNFPPNHPARDMQDTFFITRGQ
EEGDVLLRTHTSPVQVRVMLDEKPPIRVICPGKVYRNEAISARSYCVFHQLEGLYIDKNVSFADLKATIYSFARQMFGSD
VKLRFRPSFFPFTEPSAEVDVTCYLCGGKGCRVCKKSGWLEIMGCGMVHPNVMRNCGIDPEEWTGYAFGMGVDRTALLRY
KIDDIRLFFENDVRMLSQFMA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=276, Percent_Identity=28.9855072463768, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI5729820, Length=240, Percent_Identity=29.5833333333333, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1788007, Length=328, Percent_Identity=45.4268292682927, Blast_Score=296, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI32563657, Length=275, Percent_Identity=28.7272727272727, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17508957, Length=275, Percent_Identity=28.7272727272727, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32566635, Length=286, Percent_Identity=27.6223776223776, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321087, Length=275, Percent_Identity=28.3636363636364, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=100, Percent_Identity=37, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18858079, Length=268, Percent_Identity=29.4776119402985, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17137424, Length=335, Percent_Identity=25.0746268656716, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 38500; Mature: 38500

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METSIQQLQQEIDGYQIRNAKELEAFKLEFTVRKGKIAGLFGQLKTVDSADRPRIGQLLN
CCHHHHHHHHHCCCEEECCCCCCEEEEEEEEEECCCEEHHHHHHHCCCCCCCCHHHHHHH
ALKLSAEAKVADAESLFAQNAETEAPALDLSLPGRRSFLGSEHPVQKVLGDMKRIFTAMG
HHHHCCCCCCCCHHHHHHCCCCCCCCEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHC
FGIATGPELELDEYNFDRLNFPPNHPARDMQDTFFITRGQEEGDVLLRTHTSPVQVRVML
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCEEEEEEE
DEKPPIRVICPGKVYRNEAISARSYCVFHQLEGLYIDKNVSFADLKATIYSFARQMFGSD
CCCCCEEEECCCHHHHCCCCCHHHEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCC
VKLRFRPSFFPFTEPSAEVDVTCYLCGGKGCRVCKKSGWLEIMGCGMVHPNVMRNCGIDP
EEEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHCCCCEEEEECCCCCHHHHHHCCCCH
EEWTGYAFGMGVDRTALLRYKIDDIRLFFENDVRMLSQFMA
HHCCCEEEECCCCCEEEEEEEEEEEEEEEHHHHHHHHHHCC
>Mature Secondary Structure
METSIQQLQQEIDGYQIRNAKELEAFKLEFTVRKGKIAGLFGQLKTVDSADRPRIGQLLN
CCHHHHHHHHHCCCEEECCCCCCEEEEEEEEEECCCEEHHHHHHHCCCCCCCCHHHHHHH
ALKLSAEAKVADAESLFAQNAETEAPALDLSLPGRRSFLGSEHPVQKVLGDMKRIFTAMG
HHHHCCCCCCCCHHHHHHCCCCCCCCEEEECCCCCHHHCCCCCCHHHHHHHHHHHHHHHC
FGIATGPELELDEYNFDRLNFPPNHPARDMQDTFFITRGQEEGDVLLRTHTSPVQVRVML
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCEEEEEEE
DEKPPIRVICPGKVYRNEAISARSYCVFHQLEGLYIDKNVSFADLKATIYSFARQMFGSD
CCCCCEEEECCCHHHHCCCCCHHHEEEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCC
VKLRFRPSFFPFTEPSAEVDVTCYLCGGKGCRVCKKSGWLEIMGCGMVHPNVMRNCGIDP
EEEEECCCCCCCCCCCCCEEEEEEEECCCCCCHHHCCCCEEEEECCCCCHHHHHHCCCCH
EEWTGYAFGMGVDRTALLRYKIDDIRLFFENDVRMLSQFMA
HHCCCEEEECCCCCEEEEEEEEEEEEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA