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The map label for this gene is ecoRIIR [H]

Identifier: 21674931

GI number: 21674931

Start: 2007573

End: 2007839

Strand: Reverse

Name: ecoRIIR [H]

Synonym: CT2122

Alternate gene names: 21674931

Gene position: 2007839-2007573 (Counterclockwise)

Preceding gene: 21674940

Following gene: 21674919

Centisome position: 93.17

GC content: 49.81

Gene sequence:

>267_bases
ATGCTGGCAGTCAAAACCACCTGCAAGGACAGGTGGCGGCAGGTACTCAACGAGGCGAACCGAATTGGCAAAAAACACCT
CCTGACCGTTCAGCAAGGTATTTCACTGAACCAGTTTCGTGAAATGCGTGCGCATGATGTGCAACTCGTCGTTCCTGCCG
ACATCATCAAATTGTACCACAAGGATATTCGTTCGGAGATCATGACGCTTGAGGGATTCCTGGGCGAAGTGAAAACTCTG
GTGGAAAAACCACGTAAAAGGAGCTGA

Upstream 100 bases:

>100_bases
TTCTTACCAGCCCATATCCGAACTCGGCAAGCAGCCAGACTTCATCTTTCCGTCTGCGAAGAACTCTCATGATGGGGCAT
TTCCATCTGAAAACCTGCGA

Downstream 100 bases:

>100_bases
AAATATCATTCAACAAACATCATCAAGCTTGCTCGTCAATTCTTGAAAATCAAGCAGAGATGAATCATAGCAGTAACCAG
AAAATCATGAACGTTCATCG

Product: restriction endonuclease-related protein

Products: NA

Alternate protein names: R.EcoRII; Endonuclease EcoRII; Type II restriction enzyme EcoRII [H]

Number of amino acids: Translated: 88; Mature: 88

Protein sequence:

>88_residues
MLAVKTTCKDRWRQVLNEANRIGKKHLLTVQQGISLNQFREMRAHDVQLVVPADIIKLYHKDIRSEIMTLEGFLGEVKTL
VEKPRKRS

Sequences:

>Translated_88_residues
MLAVKTTCKDRWRQVLNEANRIGKKHLLTVQQGISLNQFREMRAHDVQLVVPADIIKLYHKDIRSEIMTLEGFLGEVKTL
VEKPRKRS
>Mature_88_residues
MLAVKTTCKDRWRQVLNEANRIGKKHLLTVQQGISLNQFREMRAHDVQLVVPADIIKLYHKDIRSEIMTLEGFLGEVKTL
VEKPRKRS

Specific function: Recognizes the double-stranded sequence CCWGG and cleaves before C-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011335
- InterPro:   IPR015109
- InterPro:   IPR015300 [H]

Pfam domain/function: PF09019 EcoRII-C; PF09217 EcoRII-N [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 10268; Mature: 10268

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAVKTTCKDRWRQVLNEANRIGKKHLLTVQQGISLNQFREMRAHDVQLVVPADIIKLYH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEECHHHHHHHHH
KDIRSEIMTLEGFLGEVKTLVEKPRKRS
HHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLAVKTTCKDRWRQVLNEANRIGKKHLLTVQQGISLNQFREMRAHDVQLVVPADIIKLYH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEECHHHHHHHHH
KDIRSEIMTLEGFLGEVKTLVEKPRKRS
HHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2104830; 2597679; 2612358; 8392701 [H]