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The map label for this gene is trmE

Identifier: 21674894

GI number: 21674894

Start: 1974469

End: 1975890

Strand: Reverse

Name: trmE

Synonym: CT2084

Alternate gene names: 21674894

Gene position: 1975890-1974469 (Counterclockwise)

Preceding gene: 21674895

Following gene: 21674893

Centisome position: 91.69

GC content: 62.24

Gene sequence:

>1422_bases
ATGTCACCTTCCGATCTTCATCTTCCCGTTCCCGGCCACCCCATCGCCGCCATTGCCACGCCGGTCGGTGTCGGGGCGCT
CGCCATCGTGCGCATCAGCGGCGCGGGGGTACTCGACCTGGCCGACCGCGTGTTCCGCAAAGTGCACGGCAGCGGCAAGC
TCGCCGAGGCCGCCGGGTACACCGCGCACTTCGGACGGCTCTACGACGGCGAGGAGATGGTGGATGAGGTGATTGCGCTG
GTGTTCCGCGCGCCGCGCTCATTCACCGCCGAACAGATGGTGGAGTTTACTTGCCATGGCGGGCCGGTGGTGGTGGGGCG
CGTGCTGCGGCTGATGCTCGACAACGGCTGCCGCCTGGCCGAGCCGGGTGAGTTCACGCGCCGCGCCTTCCTCAACGGGC
GCATCGATCTGCTGCAGGCTGAAGCGATCGGCGAAATGATCCACGCCCGCACCGAATCGGCCTACCGCACGGCGGTCAGC
CAGATGAAGGGCGACCTCTCGGTGAGGCTCGGTGGATTGCGCGAACAGCTCATCCGCTCCTGCGCCCTCATTGAACTCGA
ACTCGACTTCAGCGAGGAAGATGTAGAATTCCAGAGCCGCGACGAACTGACCATGCAAATCGAAACGCTCCGTAGCGAAG
TGAACCGGCTCATCGACTCCTACCAGCACGGCCGGATTGTCAGCGAAGGGGTCTCGACGGTCATCGCCGGGAAGCCGAAC
GCCGGTAAGTCCACGCTGCTCAACACGTTACTTGGTCAGGAACGCGCCATCGTCAGCCACATGCCCGGCACCACGCGCGA
CTACATCGAGGAGTGTTTCATTCACGACAAGACCATGTTTCGCCTCACCGACACCGCCGGACTGCGCGAAGCGGGCGAAG
AGATCGAGCACGAAGGCATCCGCCGCAGCCGCATGAAGATGGCCGAAGCCGACCTGATTCTCTACCTGCTCGACCTCGGC
ACGGAGCGGCTCGACGATGAACTTACCGAAATCCGCGAACTGAAAGCGGCTCACCCCGCAGCGAAATTCCTCACCGTCGC
CAACAAGCTCGACCGCGCCGCAAACGCAGACGCCCTCATCCGCGCCATCGCCGACGGTACCGGCACGGAAGTCATCGGTA
TCTCCGCACTCAACGGAGACGGTATCGACACGCTCAAGCAGCACATGGGCGATCTCGTCAAAAACCTCGACAAACTCCAC
GAAGCGAGCGTGCTCGTTACCAGCCTCCGCCACTACGAAGCCCTGCGCAACGCCTCCGATGCTCTGCAAAACGCCCTCGA
ACTCATCGCGCATGAGAGCGAGACCGAACTCATCGCCTTCGAACTCCGAGCCGCCCTCGACTACGTCGGCCAAATCACTG
GAAAGGTGGTGAATGAAGAGGTCTTGAATACGATTTTTGACAAGTTTTGTATCGGGAAATAG

Upstream 100 bases:

>100_bases
CCTCGCCCAGCTGATCGTCTCGCTTGTTGCATTGCCAGACGGCATGCTTCCCGACGAAATGACCGTTCGCCCTTTGTAAC
TCATAATTTTGTTATGACTT

Downstream 100 bases:

>100_bases
ACAAAGAACATTTACGACTTTACCACCATGCTTGTTTGTGATTTCAAAACCACCTGCCTGAACTGGGTGAATGTCGATGA
TGTCGTGATGGGCGGTGTAT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSPSDLHLPVPGHPIAAIATPVGVGALAIVRISGAGVLDLADRVFRKVHGSGKLAEAAGYTAHFGRLYDGEEMVDEVIAL
VFRAPRSFTAEQMVEFTCHGGPVVVGRVLRLMLDNGCRLAEPGEFTRRAFLNGRIDLLQAEAIGEMIHARTESAYRTAVS
QMKGDLSVRLGGLREQLIRSCALIELELDFSEEDVEFQSRDELTMQIETLRSEVNRLIDSYQHGRIVSEGVSTVIAGKPN
AGKSTLLNTLLGQERAIVSHMPGTTRDYIEECFIHDKTMFRLTDTAGLREAGEEIEHEGIRRSRMKMAEADLILYLLDLG
TERLDDELTEIRELKAAHPAAKFLTVANKLDRAANADALIRAIADGTGTEVIGISALNGDGIDTLKQHMGDLVKNLDKLH
EASVLVTSLRHYEALRNASDALQNALELIAHESETELIAFELRAALDYVGQITGKVVNEEVLNTIFDKFCIGK

Sequences:

>Translated_473_residues
MSPSDLHLPVPGHPIAAIATPVGVGALAIVRISGAGVLDLADRVFRKVHGSGKLAEAAGYTAHFGRLYDGEEMVDEVIAL
VFRAPRSFTAEQMVEFTCHGGPVVVGRVLRLMLDNGCRLAEPGEFTRRAFLNGRIDLLQAEAIGEMIHARTESAYRTAVS
QMKGDLSVRLGGLREQLIRSCALIELELDFSEEDVEFQSRDELTMQIETLRSEVNRLIDSYQHGRIVSEGVSTVIAGKPN
AGKSTLLNTLLGQERAIVSHMPGTTRDYIEECFIHDKTMFRLTDTAGLREAGEEIEHEGIRRSRMKMAEADLILYLLDLG
TERLDDELTEIRELKAAHPAAKFLTVANKLDRAANADALIRAIADGTGTEVIGISALNGDGIDTLKQHMGDLVKNLDKLH
EASVLVTSLRHYEALRNASDALQNALELIAHESETELIAFELRAALDYVGQITGKVVNEEVLNTIFDKFCIGK
>Mature_472_residues
SPSDLHLPVPGHPIAAIATPVGVGALAIVRISGAGVLDLADRVFRKVHGSGKLAEAAGYTAHFGRLYDGEEMVDEVIALV
FRAPRSFTAEQMVEFTCHGGPVVVGRVLRLMLDNGCRLAEPGEFTRRAFLNGRIDLLQAEAIGEMIHARTESAYRTAVSQ
MKGDLSVRLGGLREQLIRSCALIELELDFSEEDVEFQSRDELTMQIETLRSEVNRLIDSYQHGRIVSEGVSTVIAGKPNA
GKSTLLNTLLGQERAIVSHMPGTTRDYIEECFIHDKTMFRLTDTAGLREAGEEIEHEGIRRSRMKMAEADLILYLLDLGT
ERLDDELTEIRELKAAHPAAKFLTVANKLDRAANADALIRAIADGTGTEVIGISALNGDGIDTLKQHMGDLVKNLDKLHE
ASVLVTSLRHYEALRNASDALQNALELIAHESETELIAFELRAALDYVGQITGKVVNEEVLNTIFDKFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=412, Percent_Identity=34.7087378640777, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI193082988, Length=444, Percent_Identity=32.2072072072072, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI193082993, Length=405, Percent_Identity=33.0864197530864, Blast_Score=186, Evalue=4e-47,
Organism=Escherichia coli, GI2367268, Length=462, Percent_Identity=36.5800865800866, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI87082120, Length=91, Percent_Identity=37.3626373626374, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=463, Percent_Identity=31.1015118790497, Blast_Score=195, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6323665, Length=506, Percent_Identity=30.0395256916996, Blast_Score=202, Evalue=9e-53,
Organism=Drosophila melanogaster, GI45550813, Length=471, Percent_Identity=29.2993630573248, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_CHLTE (Q8KAS1)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662959.1
- PDB:   3GEE
- PDB:   3GEI
- PDBsum:   3GEE
- PDBsum:   3GEI
- ProteinModelPortal:   Q8KAS1
- SMR:   Q8KAS1
- DIP:   DIP-48420N
- GeneID:   1007478
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT2084
- NMPDR:   fig|194439.1.peg.2053
- TIGR:   CT2084
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   CTEP194439:CT_2084-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51818; Mature: 51687

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: BINDING 31-31 BINDING 95-95 BINDING 134-134 BINDING 473-473

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSDLHLPVPGHPIAAIATPVGVGALAIVRISGAGVLDLADRVFRKVHGSGKLAEAAGY
CCCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHCCC
TAHFGRLYDGEEMVDEVIALVFRAPRSFTAEQMVEFTCHGGPVVVGRVLRLMLDNGCRLA
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
EPGEFTRRAFLNGRIDLLQAEAIGEMIHARTESAYRTAVSQMKGDLSVRLGGLREQLIRS
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECHHHHHHHHH
CALIELELDFSEEDVEFQSRDELTMQIETLRSEVNRLIDSYQHGRIVSEGVSTVIAGKPN
HHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHEEECCCCC
AGKSTLLNTLLGQERAIVSHMPGTTRDYIEECFIHDKTMFRLTDTAGLREAGEEIEHEGI
CCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCHHEEEECCHHHHHHHHHHHHHHH
RRSRMKMAEADLILYLLDLGTERLDDELTEIRELKAAHPAAKFLTVANKLDRAANADALI
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHH
RAIADGTGTEVIGISALNGDGIDTLKQHMGDLVKNLDKLHEASVLVTSLRHYEALRNASD
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALQNALELIAHESETELIAFELRAALDYVGQITGKVVNEEVLNTIFDKFCIGK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPSDLHLPVPGHPIAAIATPVGVGALAIVRISGAGVLDLADRVFRKVHGSGKLAEAAGY
CCCCCCCCCCCCCHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHCCC
TAHFGRLYDGEEMVDEVIALVFRAPRSFTAEQMVEFTCHGGPVVVGRVLRLMLDNGCRLA
HHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
EPGEFTRRAFLNGRIDLLQAEAIGEMIHARTESAYRTAVSQMKGDLSVRLGGLREQLIRS
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECHHHHHHHHH
CALIELELDFSEEDVEFQSRDELTMQIETLRSEVNRLIDSYQHGRIVSEGVSTVIAGKPN
HHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCHHEEECCCCC
AGKSTLLNTLLGQERAIVSHMPGTTRDYIEECFIHDKTMFRLTDTAGLREAGEEIEHEGI
CCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHCCCHHEEEECCHHHHHHHHHHHHHHH
RRSRMKMAEADLILYLLDLGTERLDDELTEIRELKAAHPAAKFLTVANKLDRAANADALI
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHH
RAIADGTGTEVIGISALNGDGIDTLKQHMGDLVKNLDKLHEASVLVTSLRHYEALRNASD
HHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALQNALELIAHESETELIAFELRAALDYVGQITGKVVNEEVLNTIFDKFCIGK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901