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The map label for this gene is rsmE [H]

Identifier: 21674816

GI number: 21674816

Start: 1900089

End: 1900835

Strand: Reverse

Name: rsmE [H]

Synonym: CT2005

Alternate gene names: 21674816

Gene position: 1900835-1900089 (Counterclockwise)

Preceding gene: 21674817

Following gene: 21674815

Centisome position: 88.21

GC content: 61.31

Gene sequence:

>747_bases
ATGGATCTTTTTTACGTTTTGCCGCACCAGCTCGATCTTGAACATGCCTGGGCGATCATCGACGGTGAAGAGTTCCACCA
TCTCGCCCGCGTGTTGCGCTGCCAGCCGGGCGATGTCGTCCCGATTACAGACGGCGCGGGCTTTGCTGCGGAGCTGATGG
TCGGGTCAATCGGCAAGCACTCGCTCGATGGCGCGATCTGCAACCCGCGCACGGTGCCGCCGCCGAAAACGCAGGTCACT
GTGGCGTTGTCGCTCCTGAAGTCGCCGCAGCGCTTCGATCTTTTCCTCGAAAAGGCGACTGAACTCGGCGCTGCCCGCAT
CGTGCCAATGATCACGAAGCGCACCGTTTCGACGCCCGATTCCGGAAAGGTTGGGCGCAAGCTCGAACGGTGGCGCGGCA
TCGTGCAGTCGGCAGCCCGCCAGAGCCGCCGGTACCATCTTCCCGAACTTATGAGTCCACTCACCTTCCGGGAGGCGCTT
GTTCTCGACGGATATGATCTGCGCCTGATTGCTCACGAAAGCGAGAATGCATTTCCTTCTTTCGAGCCAGCCGGGAAAAA
GATACTCTTTCTCGTTGGTGGCGAGGGGGGCTTTACCGACGCCGAAGTCGCAGATGCTGTCGAGGCCGGATTCACGCCAC
TCTCCTTCGGCGAATCGGTGCTGCGGGCCGAGACCGCTGGAATCTTCGCAGTTGCCCTTGTCCGGGCCAGGCTGCTGGCA
GAGGCCGATCCCGCAAAGCGTCTCTGA

Upstream 100 bases:

>100_bases
CGGCGCGACAATGGTGTCGGCGGCAAAGGTGCTGAAAGCCGCCGGCGCGGTCGAGGTGGCTTTTGCGACCGTTGCCCTCA
CCGAAAAGGAGTAGCCGGAG

Downstream 100 bases:

>100_bases
TCCAGGCCCGTTGCGGTCGAATCACTTTTTGCAGTGATGCGGCGAGCAGGTTAAATTGAGGGAGCTTTTGCAACGATGGA
TGCCCCTTATTGCCATGGTA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MDLFYVLPHQLDLEHAWAIIDGEEFHHLARVLRCQPGDVVPITDGAGFAAELMVGSIGKHSLDGAICNPRTVPPPKTQVT
VALSLLKSPQRFDLFLEKATELGAARIVPMITKRTVSTPDSGKVGRKLERWRGIVQSAARQSRRYHLPELMSPLTFREAL
VLDGYDLRLIAHESENAFPSFEPAGKKILFLVGGEGGFTDAEVADAVEAGFTPLSFGESVLRAETAGIFAVALVRARLLA
EADPAKRL

Sequences:

>Translated_248_residues
MDLFYVLPHQLDLEHAWAIIDGEEFHHLARVLRCQPGDVVPITDGAGFAAELMVGSIGKHSLDGAICNPRTVPPPKTQVT
VALSLLKSPQRFDLFLEKATELGAARIVPMITKRTVSTPDSGKVGRKLERWRGIVQSAARQSRRYHLPELMSPLTFREAL
VLDGYDLRLIAHESENAFPSFEPAGKKILFLVGGEGGFTDAEVADAVEAGFTPLSFGESVLRAETAGIFAVALVRARLLA
EADPAKRL
>Mature_248_residues
MDLFYVLPHQLDLEHAWAIIDGEEFHHLARVLRCQPGDVVPITDGAGFAAELMVGSIGKHSLDGAICNPRTVPPPKTQVT
VALSLLKSPQRFDLFLEKATELGAARIVPMITKRTVSTPDSGKVGRKLERWRGIVQSAARQSRRYHLPELMSPLTFREAL
VLDGYDLRLIAHESENAFPSFEPAGKKILFLVGGEGGFTDAEVADAVEAGFTPLSFGESVLRAETAGIFAVALVRARLLA
EADPAKRL

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=215, Percent_Identity=24.1860465116279, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001206
- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27019; Mature: 27019

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLFYVLPHQLDLEHAWAIIDGEEFHHLARVLRCQPGDVVPITDGAGFAAELMVGSIGKH
CCEEEECCCEECCCCEEEEECCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCCC
SLDGAICNPRTVPPPKTQVTVALSLLKSPQRFDLFLEKATELGAARIVPMITKRTVSTPD
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
SGKVGRKLERWRGIVQSAARQSRRYHLPELMSPLTFREALVLDGYDLRLIAHESENAFPS
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHEECCCEEEEEEECCCCCCCC
FEPAGKKILFLVGGEGGFTDAEVADAVEAGFTPLSFGESVLRAETAGIFAVALVRARLLA
CCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EADPAKRL
CCCCCCCC
>Mature Secondary Structure
MDLFYVLPHQLDLEHAWAIIDGEEFHHLARVLRCQPGDVVPITDGAGFAAELMVGSIGKH
CCEEEECCCEECCCCEEEEECCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHCCCCC
SLDGAICNPRTVPPPKTQVTVALSLLKSPQRFDLFLEKATELGAARIVPMITKRTVSTPD
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
SGKVGRKLERWRGIVQSAARQSRRYHLPELMSPLTFREALVLDGYDLRLIAHESENAFPS
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHEECCCEEEEEEECCCCCCCC
FEPAGKKILFLVGGEGGFTDAEVADAVEAGFTPLSFGESVLRAETAGIFAVALVRARLLA
CCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EADPAKRL
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]