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The map label for this gene is pcaD [H]

Identifier: 21674720

GI number: 21674720

Start: 1804908

End: 1805678

Strand: Direct

Name: pcaD [H]

Synonym: CT1908

Alternate gene names: 21674720

Gene position: 1804908-1805678 (Clockwise)

Preceding gene: 21674719

Following gene: 21674723

Centisome position: 83.76

GC content: 62.52

Gene sequence:

>771_bases
ATGCTGACGTTCAATGGAGCAGCGGGCGGTGACGCCGGTAATGTTTTGCTATTGCACGCCTTCCCGGTCTCGTCGCAGAT
GTGGGAGCCGCAGCTCGCGCCGCTGGCCGAGTCGGGCTACCGGGTGATCGCCCCCGCCGTGTACGGATTCGAGTCAACGC
CGTCACGCCCCGGCTGGAGCATGGATGATTACGCGCACGACCTCGCCCGCCTCATGGAAGCGCTCGGCTGGAAGAGCGCG
ACCATCGTTGGCCTCTCGATGGGCGGCTATCAGGCGATGGCCTTCTACCGGCTCTACCCGGAGCTGACAAAATCGCTGGT
GCTCTGCGACACGCGCGCCAACGCCGACACGCCGCAGGCTTTCTCCGTACGGCAGGAGTTTCGGAAAGCGGTGATGGAGA
AAGGAGCCGAAGAGGCGGCGGCGAGGATGGTGCCGAACTTCTTCGCCAAGGAGACCTACGAATCGAACCCATCGCTCGTC
GAGAAAACGCGGGAGAGCATCGTCCGCCAGGCGCCGGAGGAGATCAGCGAAGCGATGCGGGCGATCGCCGAACGCGAAGA
CTCGACAGAAATGCTGACCGAAATCACCTGCCCGACGCTTATCGTCAACGGCATGGAAGACATCGTCACCACGCCGGAAA
TCGCCGCCACCATGCACGCCCTGATTCCCGGCTCGAAGCTCGAACTGATTCCCGACGCGGGCCACCTCTCAAACCTCGAC
CAACCCGCGATCTTCAACGGAATTCTGCTGGAGCATTTGCGGAGTTTGTAA

Upstream 100 bases:

>100_bases
CGACAGCGCCCGTGACGAGGCCCGCAAGATTCTCGATGAGGCCAACAACATCATCCGCGACATTCGCGGCAGCCAGGCCA
AAGCGCAGGAGAACTAAGTC

Downstream 100 bases:

>100_bases
AAAAGGAAACCCTCAACCGGTGCGAACTTGTTGGGGGGGGGGTTGATTTTCATCTCCTGTACAGCGAACATTCTAACGCT
AAAAATACTTATTTATTATA

Product: 3-oxoadipate enol-lactonase, putative

Products: NA

Alternate protein names: 3-oxoadipate enol-lactonase I; Beta-ketoadipate enol-lactone hydrolase I; Enol-lactone hydrolase I [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLTFNGAAGGDAGNVLLLHAFPVSSQMWEPQLAPLAESGYRVIAPAVYGFESTPSRPGWSMDDYAHDLARLMEALGWKSA
TIVGLSMGGYQAMAFYRLYPELTKSLVLCDTRANADTPQAFSVRQEFRKAVMEKGAEEAAARMVPNFFAKETYESNPSLV
EKTRESIVRQAPEEISEAMRAIAEREDSTEMLTEITCPTLIVNGMEDIVTTPEIAATMHALIPGSKLELIPDAGHLSNLD
QPAIFNGILLEHLRSL

Sequences:

>Translated_256_residues
MLTFNGAAGGDAGNVLLLHAFPVSSQMWEPQLAPLAESGYRVIAPAVYGFESTPSRPGWSMDDYAHDLARLMEALGWKSA
TIVGLSMGGYQAMAFYRLYPELTKSLVLCDTRANADTPQAFSVRQEFRKAVMEKGAEEAAARMVPNFFAKETYESNPSLV
EKTRESIVRQAPEEISEAMRAIAEREDSTEMLTEITCPTLIVNGMEDIVTTPEIAATMHALIPGSKLELIPDAGHLSNLD
QPAIFNGILLEHLRSL
>Mature_256_residues
MLTFNGAAGGDAGNVLLLHAFPVSSQMWEPQLAPLAESGYRVIAPAVYGFESTPSRPGWSMDDYAHDLARLMEALGWKSA
TIVGLSMGGYQAMAFYRLYPELTKSLVLCDTRANADTPQAFSVRQEFRKAVMEKGAEEAAARMVPNFFAKETYESNPSLV
EKTRESIVRQAPEEISEAMRAIAEREDSTEMLTEITCPTLIVNGMEDIVTTPEIAATMHALIPGSKLELIPDAGHLSNLD
QPAIFNGILLEHLRSL

Specific function: Unknown

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787244, Length=246, Percent_Identity=25.609756097561, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081721, Length=267, Percent_Identity=25.4681647940075, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR012790 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.24 [H]

Molecular weight: Translated: 28024; Mature: 28024

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTFNGAAGGDAGNVLLLHAFPVSSQMWEPQLAPLAESGYRVIAPAVYGFESTPSRPGWS
CEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCEEEEHHHHCCCCCCCCCCCC
MDDYAHDLARLMEALGWKSATIVGLSMGGYQAMAFYRLYPELTKSLVLCDTRANADTPQA
HHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCHH
FSVRQEFRKAVMEKGAEEAAARMVPNFFAKETYESNPSLVEKTRESIVRQAPEEISEAMR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHH
AIAEREDSTEMLTEITCPTLIVNGMEDIVTTPEIAATMHALIPGSKLELIPDAGHLSNLD
HHHHCCCHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCCCCC
QPAIFNGILLEHLRSL
CCHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTFNGAAGGDAGNVLLLHAFPVSSQMWEPQLAPLAESGYRVIAPAVYGFESTPSRPGWS
CEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHCCCEEEEHHHHCCCCCCCCCCCC
MDDYAHDLARLMEALGWKSATIVGLSMGGYQAMAFYRLYPELTKSLVLCDTRANADTPQA
HHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCHH
FSVRQEFRKAVMEKGAEEAAARMVPNFFAKETYESNPSLVEKTRESIVRQAPEEISEAMR
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHH
AIAEREDSTEMLTEITCPTLIVNGMEDIVTTPEIAATMHALIPGSKLELIPDAGHLSNLD
HHHHCCCHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCCCCC
QPAIFNGILLEHLRSL
CCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8181753 [H]