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The map label for this gene is hsdM-2 [H]

Identifier: 21674693

GI number: 21674693

Start: 1780561

End: 1782117

Strand: Reverse

Name: hsdM-2 [H]

Synonym: CT1881

Alternate gene names: 21674693

Gene position: 1782117-1780561 (Counterclockwise)

Preceding gene: 21674694

Following gene: 21674692

Centisome position: 82.7

GC content: 59.34

Gene sequence:

>1557_bases
ATGACCAGCATCCAACAACGCGCCGAACTGCAACGCCGCATCTGGCAGATCGCCAACGATGTGCGTGGCACCGTCGATGG
CTGGGATTTCAAGCAATATGTGCTCGGGGCGCTGTTCTACCGCTTCATCAGCGAGAACTTTGCCGCCCACATGGAAGCCG
GGGACGACGGCATCCGCTACGCCGAGCTGCCCGACAGCGTCATCACCCCCGAACTCAAGGATGACGCCATCAAGACCAAG
GGCTACTTCATCTACCCCAGCCAGTTGTTCGCAAACGTCGTGGCCAGGGCCAACACCAACGACAGCCTCAACACCGACCT
CGCCGCCATCTTTACCGCCATTGAAAGCTCGGCCAACGGTTATCCGTCGGAGCAGGACATCAAGGGGCTGTTTGCCGACT
TCGACACCACCAGCAACCGCCTGGGCAATACGGTCAAAGACAAAAACCAACGTCTCGCCGCTGTGCTCAAGGGCGTGGCC
GAGCTGGATTTCGGCCCCTTCGACGATGCCCACATCGACCTGTTCGGCGACGCCTACGAATTCCTCATCTCCAACTACGC
CGCCAACGCGGGTAAATCGGGCGGCGAGTTCTTCACGCCGCAGCACGTCTCGCGGCTGATCGCACGGCTCGCCCTGCACG
GGCAAAAGAGCGTCAACAAGATTTATGACCCGGCCTGCGGCTCCGGCTCGCTGCTCTTGCAAGCCAAAAAACCGTTCGAT
GAACGCCTCATCGAAGACGGTTTTTTTGGGCAGGAAAGCAACCACACCACCTACAACCTGGCGCGGATGAACATGTTCCT
GCACAACATCAACTACGACAAGTTCAACATCCAGCTCGGCAACACCTTGCTGGAGCCGCACTTCGCCGACGAAAAGCCCT
TTGATGCCATCGTCTCCAACCCGCCGTATTCGGTGAAGTGGATCGGCAGCGACGACCCCACCCTCATCAACGACGAACGC
TTTGCCCCGGCGGGCGTGCTGGCCCCCAAATCCAAGGCCGACTTTGCCTTTGTGCTGCACGCGCTGCATTACCTCTCAGC
AAAAGGCAGAGCGGCCATCGTTTGCTTTCCGGGCATCTTCTACCGGGGCGGGGCGGAAGCCAAAATCCGCCAGTACCTGG
TGGACAACAACTACGTCGAGACGGTGATTGCGCTTGCCCCCAATCTCTTTTTTGGTACCACCATCGCCGTCAACATTCTG
GTGCTCTCCAAACACAAGCCCGACACCACCACCCAGTTCATCGACGCCAGCGCCCTGTTCAAGAAAGAAACCAACAACAA
CGTGCTGCTGGACGAGCACATCGAGCAGATCATGGCCGTCTTTGCCAGCAAGGAGGAGGTGCCGCACGTTGCCCAGTCCG
TGCCGCTGGAACGCATTGCCGCCAACAACTACAACCTGTCCGTCAGCAGCTACGTCGAAGCCCGAGACACCCGCGAGGTG
GTGGACATTGCCCAGCTCAACGCCGAGCTGAAGACCACCGTGGCGCGCATCGATGAGTTGCGCAAACAGATCGACGCCAT
CGTGGCCGAAATCGAGGGCGAGGAGGATGAGGCATGA

Upstream 100 bases:

>100_bases
GTTATCTTGAGCTGATCGCCGCCAAAGCCAGACGGTGAGCTGTAACTATTGTTATGCATTACATCAATCGCATATTCGAC
CGTACCCGGGGAGGAAATAA

Downstream 100 bases:

>100_bases
GCGGGCAAGTTGTGATAGGGGTCTGCTGGTGCGTTGCCAAATTTGTGCAACAGGTCGTTGCCCAATTGCCTTTGTTCTAC
AAACTGGCCGCGGCGCTTCC

Product: type I restriction system adenine methylase

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTSIQQRAELQRRIWQIANDVRGTVDGWDFKQYVLGALFYRFISENFAAHMEAGDDGIRYAELPDSVITPELKDDAIKTK
GYFIYPSQLFANVVARANTNDSLNTDLAAIFTAIESSANGYPSEQDIKGLFADFDTTSNRLGNTVKDKNQRLAAVLKGVA
ELDFGPFDDAHIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSRLIARLALHGQKSVNKIYDPACGSGSLLLQAKKPFD
ERLIEDGFFGQESNHTTYNLARMNMFLHNINYDKFNIQLGNTLLEPHFADEKPFDAIVSNPPYSVKWIGSDDPTLINDER
FAPAGVLAPKSKADFAFVLHALHYLSAKGRAAIVCFPGIFYRGGAEAKIRQYLVDNNYVETVIALAPNLFFGTTIAVNIL
VLSKHKPDTTTQFIDASALFKKETNNNVLLDEHIEQIMAVFASKEEVPHVAQSVPLERIAANNYNLSVSSYVEARDTREV
VDIAQLNAELKTTVARIDELRKQIDAIVAEIEGEEDEA

Sequences:

>Translated_518_residues
MTSIQQRAELQRRIWQIANDVRGTVDGWDFKQYVLGALFYRFISENFAAHMEAGDDGIRYAELPDSVITPELKDDAIKTK
GYFIYPSQLFANVVARANTNDSLNTDLAAIFTAIESSANGYPSEQDIKGLFADFDTTSNRLGNTVKDKNQRLAAVLKGVA
ELDFGPFDDAHIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSRLIARLALHGQKSVNKIYDPACGSGSLLLQAKKPFD
ERLIEDGFFGQESNHTTYNLARMNMFLHNINYDKFNIQLGNTLLEPHFADEKPFDAIVSNPPYSVKWIGSDDPTLINDER
FAPAGVLAPKSKADFAFVLHALHYLSAKGRAAIVCFPGIFYRGGAEAKIRQYLVDNNYVETVIALAPNLFFGTTIAVNIL
VLSKHKPDTTTQFIDASALFKKETNNNVLLDEHIEQIMAVFASKEEVPHVAQSVPLERIAANNYNLSVSSYVEARDTREV
VDIAQLNAELKTTVARIDELRKQIDAIVAEIEGEEDEA
>Mature_517_residues
TSIQQRAELQRRIWQIANDVRGTVDGWDFKQYVLGALFYRFISENFAAHMEAGDDGIRYAELPDSVITPELKDDAIKTKG
YFIYPSQLFANVVARANTNDSLNTDLAAIFTAIESSANGYPSEQDIKGLFADFDTTSNRLGNTVKDKNQRLAAVLKGVAE
LDFGPFDDAHIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSRLIARLALHGQKSVNKIYDPACGSGSLLLQAKKPFDE
RLIEDGFFGQESNHTTYNLARMNMFLHNINYDKFNIQLGNTLLEPHFADEKPFDAIVSNPPYSVKWIGSDDPTLINDERF
APAGVLAPKSKADFAFVLHALHYLSAKGRAAIVCFPGIFYRGGAEAKIRQYLVDNNYVETVIALAPNLFFGTTIAVNILV
LSKHKPDTTTQFIDASALFKKETNNNVLLDEHIEQIMAVFASKEEVPHVAQSVPLERIAANNYNLSVSSYVEARDTREVV
DIAQLNAELKTTVARIDELRKQIDAIVAEIEGEEDEA

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=330, Percent_Identity=26.969696969697, Blast_Score=93, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 57557; Mature: 57426

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIQQRAELQRRIWQIANDVRGTVDGWDFKQYVLGALFYRFISENFAAHMEAGDDGIRY
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
AELPDSVITPELKDDAIKTKGYFIYPSQLFANVVARANTNDSLNTDLAAIFTAIESSANG
ECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
YPSEQDIKGLFADFDTTSNRLGNTVKDKNQRLAAVLKGVAELDFGPFDDAHIDLFGDAYE
CCCHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHH
FLISNYAANAGKSGGEFFTPQHVSRLIARLALHGQKSVNKIYDPACGSGSLLLQAKKPFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCHH
ERLIEDGFFGQESNHTTYNLARMNMFLHNINYDKFNIQLGNTLLEPHFADEKPFDAIVSN
HHHHHCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEEEEECCEECCCCCCCCCCCHHHHCC
PPYSVKWIGSDDPTLINDERFAPAGVLAPKSKADFAFVLHALHYLSAKGRAAIVCFPGIF
CCCEEEEECCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCEE
YRGGAEAKIRQYLVDNNYVETVIALAPNLFFGTTIAVNILVLSKHKPDTTTQFIDASALF
ECCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHEEEEEEEEEECCCCCCHHHHHHHHHHH
KKETNNNVLLDEHIEQIMAVFASKEEVPHVAQSVPLERIAANNYNLSVSSYVEARDTREV
HHCCCCCEEHHHHHHHHHHHHHCHHCCCHHHHHCCHHHHHCCCCCEEHHHHHHHHHHHHH
VDIAQLNAELKTTVARIDELRKQIDAIVAEIEGEEDEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TSIQQRAELQRRIWQIANDVRGTVDGWDFKQYVLGALFYRFISENFAAHMEAGDDGIRY
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
AELPDSVITPELKDDAIKTKGYFIYPSQLFANVVARANTNDSLNTDLAAIFTAIESSANG
ECCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
YPSEQDIKGLFADFDTTSNRLGNTVKDKNQRLAAVLKGVAELDFGPFDDAHIDLFGDAYE
CCCHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHH
FLISNYAANAGKSGGEFFTPQHVSRLIARLALHGQKSVNKIYDPACGSGSLLLQAKKPFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCHH
ERLIEDGFFGQESNHTTYNLARMNMFLHNINYDKFNIQLGNTLLEPHFADEKPFDAIVSN
HHHHHCCCCCCCCCCCEEEHHHHHHHHHHCCCCEEEEEECCEECCCCCCCCCCCHHHHCC
PPYSVKWIGSDDPTLINDERFAPAGVLAPKSKADFAFVLHALHYLSAKGRAAIVCFPGIF
CCCEEEEECCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCEE
YRGGAEAKIRQYLVDNNYVETVIALAPNLFFGTTIAVNILVLSKHKPDTTTQFIDASALF
ECCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHEEEEEEEEEECCCCCCHHHHHHHHHHH
KKETNNNVLLDEHIEQIMAVFASKEEVPHVAQSVPLERIAANNYNLSVSSYVEARDTREV
HHCCCCCEEHHHHHHHHHHHHHCHHCCCHHHHHCCHHHHHCCCCCEEHHHHHHHHHHHHH
VDIAQLNAELKTTVARIDELRKQIDAIVAEIEGEEDEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]