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The map label for this gene is lysS [H]

Identifier: 21674209

GI number: 21674209

Start: 1300998

End: 1302533

Strand: Direct

Name: lysS [H]

Synonym: CT1387

Alternate gene names: 21674209

Gene position: 1300998-1302533 (Clockwise)

Preceding gene: 21674208

Following gene: 21674212

Centisome position: 60.37

GC content: 58.72

Gene sequence:

>1536_bases
ATGTCCAACGCACCAGAACAGAAGAATCCGCAGAACGATCCGTCACCCGCAGTTTCGCTCAACGACCAGATGAAGCGCCG
CTTCGAGGAGCGCACGCACCTTGCCGAAGCAGGCATCAATCCCTATCCCTATAAATTCGACGTTACAACCACCTCGAAAG
CGATCATCGACAGCTTCAGCGATGAAAATCCCGCAGATGTTTCGGTGGCCGGACGCATCATGGCCATCCGCAGGATGGGC
AAGGCGTCGTTCCTGCATATTCAGGACTCCGAGGGTCGCATCCAGATTTACCTCAAAAAGGACGACGTCGGCGAGGCTTC
GTACAACACCTTCAAGCTGCTCGACATCGGCGACATCGTGGGCGTCAGCGGTTTCACGTTCAAGACGAAAACCGGTGAAA
TCTCGGTACACGCCAGGCAATTCGAGCTGCTTGCCAAGTCGCTCCGCCCGATTCCGATTGCCAAAGAGAAAGAGGTTGAC
GGCCAGAAGGTGATTTACGATGCTTTCAGCGACCGCGAGTTGCGCTACCGCCAGCGCTATGTCGATCTGATCGTCAACCC
GGAGGTGCGCGGCACCTTCATCAAGCGGACGAAGATCGTCGCCCTGATGCGCAACTACTTCGCCTCGAACGGCTGGCTCG
AAGTGGAAACGCCGATTCTCCAGCCGATCTACGGCGGCGCGGCGGCCCGCCCCTTCACGACGCACCACAACGCGCTCGAT
ATGCAGCTCTACCTGCGCATCGCCAACGAATTGTACCTCAAGCGGCTCATCGTCGGCGGCTTCGACGGCGTGTTCGAGTT
CGCCAAGGACTTCCGCAACGAGGGCATCGACCGCTTCCACAACCCGGAATTCACCCAGGTCGAGCTCTATGTCGCCTACA
AGGATTACATCTGGATGATGGAGCTGGTCGAAGATTTACTGCACAAGGCTTGCGTAGAAGTGAACGGCAAGGATTCGACG
ATGTTCCTCGGCAACGAAATCAACCTCAAGCCACCCTTCCGCCGCCTCACCATCGCCGACTCGATCAGGGAGTACACCGG
CATGGAGATTCGCGGCAAATCGGAAGCGCAGCTCCGCGACATTGCGAAAGATTTGGGACTCGAACTCGATCCCAAGATCA
GCAGCGGCAAGATCATCGATGAAATTTTCGGCGAATTCGTCGAGCCGAAGCTCATCCAGCCGACCTTCATCACCGACTAC
CCGGAGGAGATGTCGCCGCTCGCCAAGAAGCACCGCTCGGAGCCTGGACTCGTCGAGCGCTTCGAGCTGATCGTCGGCGG
CAAGGAGGTGTGCAACTCCTTCTCCGAGCTGAACGACCCGGTCATCCAGCGCGAACGCCTCGAAGAGCAGGCCCGCCTAC
GCCAGCGCGGCGACGACGAAGCGATGATCGTTGACGAGGATTTCCTTCGCGCGCTGGAATACGGCATGCCACCCTGCGCC
GGCCTCGGCATTGGCATCGACCGCATGGTGATGCTGCTGACCGGCCAGGACTCCATCCGCGACGTCATCTTCTTCCCGCA
CATGAAGCCCGAATGA

Upstream 100 bases:

>100_bases
TGAGCACCCTGCCGTCGATCTGGATCAAAACCATGATCTCGGCCTGACCCGCGCAAGCTTCAGATAACCCGTTTTACTTT
TATCACTTCAGACCATCAGC

Downstream 100 bases:

>100_bases
ATCACGGATTGATGGAATGAAAAGCAAAAGCCCCGGCTCGGATGAAGCGCCGGGGCTTTTGCTTTTTTGGGTGAAACAAC
ACTCTTGAATACTTGACAGA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MSNAPEQKNPQNDPSPAVSLNDQMKRRFEERTHLAEAGINPYPYKFDVTTTSKAIIDSFSDENPADVSVAGRIMAIRRMG
KASFLHIQDSEGRIQIYLKKDDVGEASYNTFKLLDIGDIVGVSGFTFKTKTGEISVHARQFELLAKSLRPIPIAKEKEVD
GQKVIYDAFSDRELRYRQRYVDLIVNPEVRGTFIKRTKIVALMRNYFASNGWLEVETPILQPIYGGAAARPFTTHHNALD
MQLYLRIANELYLKRLIVGGFDGVFEFAKDFRNEGIDRFHNPEFTQVELYVAYKDYIWMMELVEDLLHKACVEVNGKDST
MFLGNEINLKPPFRRLTIADSIREYTGMEIRGKSEAQLRDIAKDLGLELDPKISSGKIIDEIFGEFVEPKLIQPTFITDY
PEEMSPLAKKHRSEPGLVERFELIVGGKEVCNSFSELNDPVIQRERLEEQARLRQRGDDEAMIVDEDFLRALEYGMPPCA
GLGIGIDRMVMLLTGQDSIRDVIFFPHMKPE

Sequences:

>Translated_511_residues
MSNAPEQKNPQNDPSPAVSLNDQMKRRFEERTHLAEAGINPYPYKFDVTTTSKAIIDSFSDENPADVSVAGRIMAIRRMG
KASFLHIQDSEGRIQIYLKKDDVGEASYNTFKLLDIGDIVGVSGFTFKTKTGEISVHARQFELLAKSLRPIPIAKEKEVD
GQKVIYDAFSDRELRYRQRYVDLIVNPEVRGTFIKRTKIVALMRNYFASNGWLEVETPILQPIYGGAAARPFTTHHNALD
MQLYLRIANELYLKRLIVGGFDGVFEFAKDFRNEGIDRFHNPEFTQVELYVAYKDYIWMMELVEDLLHKACVEVNGKDST
MFLGNEINLKPPFRRLTIADSIREYTGMEIRGKSEAQLRDIAKDLGLELDPKISSGKIIDEIFGEFVEPKLIQPTFITDY
PEEMSPLAKKHRSEPGLVERFELIVGGKEVCNSFSELNDPVIQRERLEEQARLRQRGDDEAMIVDEDFLRALEYGMPPCA
GLGIGIDRMVMLLTGQDSIRDVIFFPHMKPE
>Mature_510_residues
SNAPEQKNPQNDPSPAVSLNDQMKRRFEERTHLAEAGINPYPYKFDVTTTSKAIIDSFSDENPADVSVAGRIMAIRRMGK
ASFLHIQDSEGRIQIYLKKDDVGEASYNTFKLLDIGDIVGVSGFTFKTKTGEISVHARQFELLAKSLRPIPIAKEKEVDG
QKVIYDAFSDRELRYRQRYVDLIVNPEVRGTFIKRTKIVALMRNYFASNGWLEVETPILQPIYGGAAARPFTTHHNALDM
QLYLRIANELYLKRLIVGGFDGVFEFAKDFRNEGIDRFHNPEFTQVELYVAYKDYIWMMELVEDLLHKACVEVNGKDSTM
FLGNEINLKPPFRRLTIADSIREYTGMEIRGKSEAQLRDIAKDLGLELDPKISSGKIIDEIFGEFVEPKLIQPTFITDYP
EEMSPLAKKHRSEPGLVERFELIVGGKEVCNSFSELNDPVIQRERLEEQARLRQRGDDEAMIVDEDFLRALEYGMPPCAG
LGIGIDRMVMLLTGQDSIRDVIFFPHMKPE

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=518, Percent_Identity=41.1196911196911, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI194272210, Length=518, Percent_Identity=41.1196911196911, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI40789249, Length=290, Percent_Identity=24.8275862068966, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1790571, Length=506, Percent_Identity=46.8379446640316, Blast_Score=460, Evalue=1e-131,
Organism=Escherichia coli, GI1789256, Length=504, Percent_Identity=47.6190476190476, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=31.6923076923077, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788173, Length=304, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=498, Percent_Identity=40.9638554216867, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17535925, Length=498, Percent_Identity=40.9638554216867, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71994340, Length=498, Percent_Identity=40.9638554216867, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32566633, Length=307, Percent_Identity=26.0586319218241, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=501, Percent_Identity=39.5209580838323, Blast_Score=363, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6324256, Length=489, Percent_Identity=28.2208588957055, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24640849, Length=521, Percent_Identity=40.3071017274472, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640851, Length=521, Percent_Identity=40.3071017274472, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17136276, Length=464, Percent_Identity=23.4913793103448, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19922416, Length=489, Percent_Identity=21.6768916155419, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24584738, Length=284, Percent_Identity=25, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 58340; Mature: 58208

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAPEQKNPQNDPSPAVSLNDQMKRRFEERTHLAEAGINPYPYKFDVTTTSKAIIDSFS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHCC
DENPADVSVAGRIMAIRRMGKASFLHIQDSEGRIQIYLKKDDVGEASYNTFKLLDIGDIV
CCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCCCCCCCCEEEEEECCHHH
GVSGFTFKTKTGEISVHARQFELLAKSLRPIPIAKEKEVDGQKVIYDAFSDRELRYRQRY
CCCCEEEEECCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHH
VDLIVNPEVRGTFIKRTKIVALMRNYFASNGWLEVETPILQPIYGGAAARPFTTHHNALD
HHEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCCCCCCCCCHH
MQLYLRIANELYLKRLIVGGFDGVFEFAKDFRNEGIDRFHNPEFTQVELYVAYKDYIWMM
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEEHHHHHHHH
ELVEDLLHKACVEVNGKDSTMFLGNEINLKPPFRRLTIADSIREYTGMEIRGKSEAQLRD
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCEECCCCHHHHHH
IAKDLGLELDPKISSGKIIDEIFGEFVEPKLIQPTFITDYPEEMSPLAKKHRSEPGLVER
HHHHCCCEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHH
FELIVGGKEVCNSFSELNDPVIQRERLEEQARLRQRGDDEAMIVDEDFLRALEYGMPPCA
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHCCCCCC
GLGIGIDRMVMLLTGQDSIRDVIFFPHMKPE
CCCCCHHHEEEEEECCCCCCCEEECCCCCCC
>Mature Secondary Structure 
SNAPEQKNPQNDPSPAVSLNDQMKRRFEERTHLAEAGINPYPYKFDVTTTSKAIIDSFS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHCC
DENPADVSVAGRIMAIRRMGKASFLHIQDSEGRIQIYLKKDDVGEASYNTFKLLDIGDIV
CCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCCCCCCCCEEEEEECCHHH
GVSGFTFKTKTGEISVHARQFELLAKSLRPIPIAKEKEVDGQKVIYDAFSDRELRYRQRY
CCCCEEEEECCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHH
VDLIVNPEVRGTFIKRTKIVALMRNYFASNGWLEVETPILQPIYGGAAARPFTTHHNALD
HHEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHCCCCCCCCCCCCCCCHH
MQLYLRIANELYLKRLIVGGFDGVFEFAKDFRNEGIDRFHNPEFTQVELYVAYKDYIWMM
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCCCCCEEEEEEEEHHHHHHHH
ELVEDLLHKACVEVNGKDSTMFLGNEINLKPPFRRLTIADSIREYTGMEIRGKSEAQLRD
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCEECCCCHHHHHH
IAKDLGLELDPKISSGKIIDEIFGEFVEPKLIQPTFITDYPEEMSPLAKKHRSEPGLVER
HHHHCCCEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHH
FELIVGGKEVCNSFSELNDPVIQRERLEEQARLRQRGDDEAMIVDEDFLRALEYGMPPCA
HHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHCCCCCC
GLGIGIDRMVMLLTGQDSIRDVIFFPHMKPE
CCCCCHHHEEEEEECCCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA