Click here to switch to the map view.

The map label for this gene is moaA

Identifier: 21674155

GI number: 21674155

Start: 1254842

End: 1255834

Strand: Direct

Name: moaA

Synonym: CT1333

Alternate gene names: 21674155

Gene position: 1254842-1255834 (Clockwise)

Preceding gene: 21674154

Following gene: 21674166

Centisome position: 58.23

GC content: 60.93

Gene sequence:

>993_bases
ATGAATCGCGAATCCGCAAAACAGCTCGTGCTGACCGACCGCTTCGGGCGCACGGTTGATTACGTGCGTATCGCCGTCAC
CAGCGCCTGCAACCTGCGCTGTACCTACTGCCTGAAGGAGGACGCGCCCACGCAAACGCAACAACTCGACGTCGTCGAAA
CCTCAAAGCTCATCGCACTCTTAGCCGGAATGGGTGTCAGGAAAATCCGCTTCACCGGCGGTGAGCCGCTGCTCCATCCA
AGCATCCCGGAACTGGTGAGGATCGCCAAAGCGACGCCCGGCATCGACACCGTCTGCATCACCACCAACGGCGTGCTGCT
CGACCGGCAACTCGACGCGCTTGTCGAAGCCGGGCTGGACGGCGTCAACCTCAGCCTCGACACCCTCGACCGCGAAAAAT
TCACATCGATCACCCGGCGCGACCGCTTTGAACAAGTCAGCAAAGCGCTCGACCGCCTGCTCGCCACGCCCAGCCTCACA
GTCAAGCTCAACACGCTCATGCTCCGGGGCATCAACAACGACGAAATCCCGGCCTTCGTCGAACTGACGCGCGAACACGA
CCTGACCGTTCGCTTCATGGAACTGCAACCCTTCGACGACCACCAGATCTGGCGCACCGGACGCTTCATGGGCGCCGAGA
GGATCCGCGAGCGGCTCGCCGACGCCTACCCGGAACTCGAAGCCATCACAGGCCACTCGACCGAGCACTACAGCTTCAGC
CTGCCCGGCCACCGGGGATCGATCGCCATCATCCCGGCCTTCTCCCGCAACTTTTGCAGCAGCTGCTCGAAGCTCAGGAT
CACCGCCGACGCCCGGCTGATCAGCTGCCTCTACCACCACGAAAGCATCGACCTCGCGCCAGCCCTGAAGGGTGAAATGA
ATGAAGTGGAGCTGAAAAAGCGGATCATCGAAGCTGTGCAGCAAAAACCCAAAGACGGCCTGAAAAGCAGCCACGACACC
GCTGCATCAAGCATGTCGCAAATTGGAGGTTAA

Upstream 100 bases:

>100_bases
GGGTACAGTTCATCGAGCCGAATGATGCGTCGAAGCTGCGCAACGTGAACGACCCTGCGGCGATGGACGACGCACTCCGG
GCAATCAGCAAGGGCGGCCA

Downstream 100 bases:

>100_bases
ACTCCGAAGAATCGAACAGATCGGTCAGATCAGTCTGATCCGGCTGATCTGTCGGAAACCACCACCACCGGACTTCCCGG
TGCTACGTGGCCACCGGCGA

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MNRESAKQLVLTDRFGRTVDYVRIAVTSACNLRCTYCLKEDAPTQTQQLDVVETSKLIALLAGMGVRKIRFTGGEPLLHP
SIPELVRIAKATPGIDTVCITTNGVLLDRQLDALVEAGLDGVNLSLDTLDREKFTSITRRDRFEQVSKALDRLLATPSLT
VKLNTLMLRGINNDEIPAFVELTREHDLTVRFMELQPFDDHQIWRTGRFMGAERIRERLADAYPELEAITGHSTEHYSFS
LPGHRGSIAIIPAFSRNFCSSCSKLRITADARLISCLYHHESIDLAPALKGEMNEVELKKRIIEAVQQKPKDGLKSSHDT
AASSMSQIGG

Sequences:

>Translated_330_residues
MNRESAKQLVLTDRFGRTVDYVRIAVTSACNLRCTYCLKEDAPTQTQQLDVVETSKLIALLAGMGVRKIRFTGGEPLLHP
SIPELVRIAKATPGIDTVCITTNGVLLDRQLDALVEAGLDGVNLSLDTLDREKFTSITRRDRFEQVSKALDRLLATPSLT
VKLNTLMLRGINNDEIPAFVELTREHDLTVRFMELQPFDDHQIWRTGRFMGAERIRERLADAYPELEAITGHSTEHYSFS
LPGHRGSIAIIPAFSRNFCSSCSKLRITADARLISCLYHHESIDLAPALKGEMNEVELKKRIIEAVQQKPKDGLKSSHDT
AASSMSQIGG
>Mature_330_residues
MNRESAKQLVLTDRFGRTVDYVRIAVTSACNLRCTYCLKEDAPTQTQQLDVVETSKLIALLAGMGVRKIRFTGGEPLLHP
SIPELVRIAKATPGIDTVCITTNGVLLDRQLDALVEAGLDGVNLSLDTLDREKFTSITRRDRFEQVSKALDRLLATPSLT
VKLNTLMLRGINNDEIPAFVELTREHDLTVRFMELQPFDDHQIWRTGRFMGAERIRERLADAYPELEAITGHSTEHYSFS
LPGHRGSIAIIPAFSRNFCSSCSKLRITADARLISCLYHHESIDLAPALKGEMNEVELKKRIIEAVQQKPKDGLKSSHDT
AASSMSQIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI22027536, Length=326, Percent_Identity=36.1963190184049, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI116734668, Length=326, Percent_Identity=36.1963190184049, Blast_Score=210, Evalue=1e-54,
Organism=Escherichia coli, GI1786999, Length=332, Percent_Identity=29.5180722891566, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17561210, Length=337, Percent_Identity=35.9050445103858, Blast_Score=195, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI32566329, Length=327, Percent_Identity=36.085626911315, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI32566331, Length=304, Percent_Identity=37.171052631579, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI28574488, Length=327, Percent_Identity=37.6146788990826, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574490, Length=306, Percent_Identity=38.562091503268, Blast_Score=201, Evalue=6e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_CHLTE (P59038)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662220.1
- ProteinModelPortal:   P59038
- SMR:   P59038
- GeneID:   1006703
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1333
- NMPDR:   fig|194439.1.peg.1314
- TIGR:   CT1333
- HOGENOM:   HBG529885
- OMA:   WRTGRFM
- ProtClustDB:   CLSK637709
- BioCyc:   CTEP194439:CT_1333-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 36760; Mature: 36760

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 23-23 BINDING 36-36 BINDING 70-70 BINDING 74-74 BINDING 101-101 BINDING 125-125 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRESAKQLVLTDRFGRTVDYVRIAVTSACNLRCTYCLKEDAPTQTQQLDVVETSKLIAL
CCHHHHHHEEEHHHCCCHHHHHEEEEECCCCCEEEEEECCCCCCCHHHCCHHHHHHHHHH
LAGMGVRKIRFTGGEPLLHPSIPELVRIAKATPGIDTVCITTNGVLLDRQLDALVEAGLD
HHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHHHHHCCCC
GVNLSLDTLDREKFTSITRRDRFEQVSKALDRLLATPSLTVKLNTLMLRGINNDEIPAFV
CCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHE
ELTREHDLTVRFMELQPFDDHQIWRTGRFMGAERIRERLADAYPELEAITGHSTEHYSFS
EEECCCCCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEECCCCCCCEEEE
LPGHRGSIAIIPAFSRNFCSSCSKLRITADARLISCLYHHESIDLAPALKGEMNEVELKK
CCCCCCCEEEEECCHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHH
RIIEAVQQKPKDGLKSSHDTAASSMSQIGG
HHHHHHHHCCHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNRESAKQLVLTDRFGRTVDYVRIAVTSACNLRCTYCLKEDAPTQTQQLDVVETSKLIAL
CCHHHHHHEEEHHHCCCHHHHHEEEEECCCCCEEEEEECCCCCCCHHHCCHHHHHHHHHH
LAGMGVRKIRFTGGEPLLHPSIPELVRIAKATPGIDTVCITTNGVLLDRQLDALVEAGLD
HHCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHHHHHCCCC
GVNLSLDTLDREKFTSITRRDRFEQVSKALDRLLATPSLTVKLNTLMLRGINNDEIPAFV
CCEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCHHE
ELTREHDLTVRFMELQPFDDHQIWRTGRFMGAERIRERLADAYPELEAITGHSTEHYSFS
EEECCCCCEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEECCCCCCCEEEE
LPGHRGSIAIIPAFSRNFCSSCSKLRITADARLISCLYHHESIDLAPALKGEMNEVELKK
CCCCCCCEEEEECCHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHH
RIIEAVQQKPKDGLKSSHDTAASSMSQIGG
HHHHHHHHCCHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901