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The map label for this gene is oprM [H]

Identifier: 21674109

GI number: 21674109

Start: 1201394

End: 1202821

Strand: Reverse

Name: oprM [H]

Synonym: CT1286

Alternate gene names: 21674109

Gene position: 1202821-1201394 (Counterclockwise)

Preceding gene: 21674110

Following gene: 21674108

Centisome position: 55.82

GC content: 63.38

Gene sequence:

>1428_bases
ATGAAAAAACGCAATCAAGTGATGAAACGCGCGGTGCCGCTGTTGTTGATGACGCTTCTCGCCACCATCGGCCTGACGGC
GTGCAGCCCGGTCGGGGAGTATCGCCGTCCCGAAGTCAGGCTTCCGGAGAGCTATCCGGGGCAGACGGCAGCGGGCAAGG
AGATGGCGGCGGTGCCCTACCGGAAGTTTTTCGCCGATCCCGATCTGCTCGAACTGATCGATAGCGCCGTGGAGAATAAC
CACGATTTGCTGATCGCGATGAAGAACATCGATTACGCGGGTAAAACGCTCGATGTGGCGAAGTTGTGGTTTCTCCCGGA
GGTCGATCTGAGCGCCACCTACGACTACTCGCGGGGCTCCCGCAACAGCGCTTCGGCTCTGAAGGGGCAGGCTCGCACCA
GCCGGAACTATACGGCGCAGCTCAACGTCTCGTGGGAGGCGGACATCTGGGCGAAGCTGCGCAACGCCAAAAAGGCCGCC
GTCGCCGAGTATCTCCGCAGCGCCGATGTGGCGCGGGCCGTGCGCACAACCCTCGTTTCGAATGTCGCTCAGGGGTACTG
GAACCTCAAGATGCTCGACGAGCAGATTGATATCACAAAGCGCAATATCGCGCTGGCCGACACGACGCTCACCATGATGC
GCTTGCAGTTCGACGCGGGCAACGTCACCAGCCTTGCCGTCGATCAGCAGGAGTCGCAGTTGCTCTCGGCGAAGGCCACC
CTCCCGAAGCTGGAGGCATCGCGCACCGCACAGGAGAACGCGCTCTCGATTCTCGCCGGACGGATGCCTGGCACGCCGAT
CAGGCGGAGTACCGGATACGCGCCCTTCGTGATGCCCGATTCCCTCGGTGCGGGCGTGCCGCTCGCGCTGCTTTCGAACC
GCCCCGACGTTCGCGCTGCCGAGGAGTCGCTCATGGAGGCGCACGCGCTCACCGGTGTCAACAAGGCGATGATGTACCCG
TCGATCACCATTACGGCGCAGGGCGGGCTGAATGCCATCGAGTCGAGCAAGTGGTTCTCGACGCCCGGCTCGCTCTTCAA
CGCGCTTCAGGGTTCGCTTTTGCAGCCGATATTCCAGCACGGCCAGCTCAAGGCGCAGTACGAGCAGTCGAAGATCAAAC
GCGACCAGGCCGAGCTGACCTTCCGCAAAACGCTCCTGGTCGCCGTGGGCGAGGTCTCGAATGCGCTTGCGCAGGTCGAG
AAAACCGGCGAGCAGGAGACGTTCGCCAAGGCTCGCGTGGCTGCCTTGCGCAAGGCGGCGGGCAATTCGCGCCTGCTCTT
CAACAGCGGCATGGCGACCTACCTCGAAGTGATCGCCGCCGAAAGCTCGCTGCTTCAGTCGGAGCTGGAACTTGCCGACG
TCCAGCGCAGCCGCCTCTCCGCGATTGCCGACCTGTACCGTGCGGTTGGCGGCGGCTGGCGGGAGTGA

Upstream 100 bases:

>100_bases
CCTTCCAGGGCTTGCAGGAGCGCTTCACCGGCCCGGCGGCGGAGATTGTCGAAGCCGGAACCTTGCTTGAAAAGCAGTTG
AAGAAGGATGGTTCGCACGA

Downstream 100 bases:

>100_bases
TGGCGGGGAAGTCAGATGACGACCATCCGTACGGAAATCGTTATCGATGCGTTGCCGGAGCAGGTCTGGGCGGCGCTGAC
CGGCTTCGGCAGTTATGGTG

Product: multidrug resistance protein FusA/NodT family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MKKRNQVMKRAVPLLLMTLLATIGLTACSPVGEYRRPEVRLPESYPGQTAAGKEMAAVPYRKFFADPDLLELIDSAVENN
HDLLIAMKNIDYAGKTLDVAKLWFLPEVDLSATYDYSRGSRNSASALKGQARTSRNYTAQLNVSWEADIWAKLRNAKKAA
VAEYLRSADVARAVRTTLVSNVAQGYWNLKMLDEQIDITKRNIALADTTLTMMRLQFDAGNVTSLAVDQQESQLLSAKAT
LPKLEASRTAQENALSILAGRMPGTPIRRSTGYAPFVMPDSLGAGVPLALLSNRPDVRAAEESLMEAHALTGVNKAMMYP
SITITAQGGLNAIESSKWFSTPGSLFNALQGSLLQPIFQHGQLKAQYEQSKIKRDQAELTFRKTLLVAVGEVSNALAQVE
KTGEQETFAKARVAALRKAAGNSRLLFNSGMATYLEVIAAESSLLQSELELADVQRSRLSAIADLYRAVGGGWRE

Sequences:

>Translated_475_residues
MKKRNQVMKRAVPLLLMTLLATIGLTACSPVGEYRRPEVRLPESYPGQTAAGKEMAAVPYRKFFADPDLLELIDSAVENN
HDLLIAMKNIDYAGKTLDVAKLWFLPEVDLSATYDYSRGSRNSASALKGQARTSRNYTAQLNVSWEADIWAKLRNAKKAA
VAEYLRSADVARAVRTTLVSNVAQGYWNLKMLDEQIDITKRNIALADTTLTMMRLQFDAGNVTSLAVDQQESQLLSAKAT
LPKLEASRTAQENALSILAGRMPGTPIRRSTGYAPFVMPDSLGAGVPLALLSNRPDVRAAEESLMEAHALTGVNKAMMYP
SITITAQGGLNAIESSKWFSTPGSLFNALQGSLLQPIFQHGQLKAQYEQSKIKRDQAELTFRKTLLVAVGEVSNALAQVE
KTGEQETFAKARVAALRKAAGNSRLLFNSGMATYLEVIAAESSLLQSELELADVQRSRLSAIADLYRAVGGGWRE
>Mature_475_residues
MKKRNQVMKRAVPLLLMTLLATIGLTACSPVGEYRRPEVRLPESYPGQTAAGKEMAAVPYRKFFADPDLLELIDSAVENN
HDLLIAMKNIDYAGKTLDVAKLWFLPEVDLSATYDYSRGSRNSASALKGQARTSRNYTAQLNVSWEADIWAKLRNAKKAA
VAEYLRSADVARAVRTTLVSNVAQGYWNLKMLDEQIDITKRNIALADTTLTMMRLQFDAGNVTSLAVDQQESQLLSAKAT
LPKLEASRTAQENALSILAGRMPGTPIRRSTGYAPFVMPDSLGAGVPLALLSNRPDVRAAEESLMEAHALTGVNKAMMYP
SITITAQGGLNAIESSKWFSTPGSLFNALQGSLLQPIFQHGQLKAQYEQSKIKRDQAELTFRKTLLVAVGEVSNALAQVE
KTGEQETFAKARVAALRKAAGNSRLLFNSGMATYLEVIAAESSLLQSELELADVQRSRLSAIADLYRAVGGGWRE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=467, Percent_Identity=27.4089935760171, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI2367348, Length=430, Percent_Identity=23.2558139534884, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51904; Mature: 51904

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRNQVMKRAVPLLLMTLLATIGLTACSPVGEYRRPEVRLPESYPGQTAAGKEMAAVPY
CCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHCCH
RKFFADPDLLELIDSAVENNHDLLIAMKNIDYAGKTLDVAKLWFLPEVDLSATYDYSRGS
HHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCEEECCCCCC
RNSASALKGQARTSRNYTAQLNVSWEADIWAKLRNAKKAAVAEYLRSADVARAVRTTLVS
CCCHHHHHCHHHCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVAQGYWNLKMLDEQIDITKRNIALADTTLTMMRLQFDAGNVTSLAVDQQESQLLSAKAT
HHHHCCCCCEEEHHHHCHHHCCEEEEHHHEEEEEEEECCCCEEEEEECHHHHHHHHHHHC
LPKLEASRTAQENALSILAGRMPGTPIRRSTGYAPFVMPDSLGAGVPLALLSNRPDVRAA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHCCCCCCHHH
EESLMEAHALTGVNKAMMYPSITITAQGGLNAIESSKWFSTPGSLFNALQGSLLQPIFQH
HHHHHHHHHHHCCCHHHCCCCEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
GQLKAQYEQSKIKRDQAELTFRKTLLVAVGEVSNALAQVEKTGEQETFAKARVAALRKAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
GNSRLLFNSGMATYLEVIAAESSLLQSELELADVQRSRLSAIADLYRAVGGGWRE
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKRNQVMKRAVPLLLMTLLATIGLTACSPVGEYRRPEVRLPESYPGQTAAGKEMAAVPY
CCHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCHHHCCH
RKFFADPDLLELIDSAVENNHDLLIAMKNIDYAGKTLDVAKLWFLPEVDLSATYDYSRGS
HHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCEEECCCCCC
RNSASALKGQARTSRNYTAQLNVSWEADIWAKLRNAKKAAVAEYLRSADVARAVRTTLVS
CCCHHHHHCHHHCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NVAQGYWNLKMLDEQIDITKRNIALADTTLTMMRLQFDAGNVTSLAVDQQESQLLSAKAT
HHHHCCCCCEEEHHHHCHHHCCEEEEHHHEEEEEEEECCCCEEEEEECHHHHHHHHHHHC
LPKLEASRTAQENALSILAGRMPGTPIRRSTGYAPFVMPDSLGAGVPLALLSNRPDVRAA
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCCCHHHCCCCCCHHH
EESLMEAHALTGVNKAMMYPSITITAQGGLNAIESSKWFSTPGSLFNALQGSLLQPIFQH
HHHHHHHHHHHCCCHHHCCCCEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
GQLKAQYEQSKIKRDQAELTFRKTLLVAVGEVSNALAQVEKTGEQETFAKARVAALRKAA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
GNSRLLFNSGMATYLEVIAAESSLLQSELELADVQRSRLSAIADLYRAVGGGWRE
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]